Streptococcus phage PH15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3GW21|B3GW21_9CAUD Uncharacterized protein OS=Streptococcus phage PH15 OX=537874 PE=4 SV=1
MM1 pKa = 7.76ADD3 pKa = 2.84IASWFEE9 pKa = 3.66ARR11 pKa = 11.84RR12 pKa = 11.84GAVTYY17 pKa = 11.44SMLGSRR23 pKa = 11.84NGTDD27 pKa = 3.23GTGDD31 pKa = 3.4CSGTISQALKK41 pKa = 10.89DD42 pKa = 3.8NGFAIQGLPSTVTLGSQLARR62 pKa = 11.84VGWVRR67 pKa = 11.84ISRR70 pKa = 11.84NEE72 pKa = 3.63DD73 pKa = 2.51WNAQRR78 pKa = 11.84NDD80 pKa = 3.11IVMMSWSADD89 pKa = 3.36MAGSGGAGGHH99 pKa = 5.44VGAMLDD105 pKa = 3.46SVNFISCDD113 pKa = 3.34YY114 pKa = 8.99STQGAPGQAINTYY127 pKa = 9.29PWDD130 pKa = 3.56YY131 pKa = 11.01YY132 pKa = 11.75YY133 pKa = 11.3NANNPTYY140 pKa = 10.46IEE142 pKa = 3.8VWRR145 pKa = 11.84YY146 pKa = 9.56NGNAPEE152 pKa = 4.38KK153 pKa = 9.19PLPNTAVAPSDD164 pKa = 3.51SRR166 pKa = 11.84KK167 pKa = 9.39PSSKK171 pKa = 10.57AYY173 pKa = 10.32YY174 pKa = 9.55LANDD178 pKa = 3.49VQLVNDD184 pKa = 4.68IYY186 pKa = 10.82QIKK189 pKa = 10.07CDD191 pKa = 3.85YY192 pKa = 9.68LCPVGFDD199 pKa = 3.08WTEE202 pKa = 3.5NGIPVSLVNWVDD214 pKa = 3.35EE215 pKa = 4.65NGNHH219 pKa = 5.92VPDD222 pKa = 5.84GEE224 pKa = 4.74DD225 pKa = 3.19KK226 pKa = 11.05DD227 pKa = 5.35FKK229 pKa = 11.21AGMYY233 pKa = 10.18FSFEE237 pKa = 3.82VDD239 pKa = 3.24EE240 pKa = 4.19VHH242 pKa = 6.66ITDD245 pKa = 4.07TGDD248 pKa = 2.6GGYY251 pKa = 10.72YY252 pKa = 9.71GGYY255 pKa = 8.98YY256 pKa = 9.64YY257 pKa = 10.9RR258 pKa = 11.84SFEE261 pKa = 3.95FGQFGTVWLSVWDD274 pKa = 4.1KK275 pKa = 11.71DD276 pKa = 5.27DD277 pKa = 4.24LVNYY281 pKa = 9.56YY282 pKa = 10.4NN283 pKa = 3.92

Molecular weight: 31.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3GW09|B3GW09_9CAUD Uncharacterized protein OS=Streptococcus phage PH15 OX=537874 PE=4 SV=1
MM1 pKa = 7.46TSIKK5 pKa = 10.66LKK7 pKa = 10.3IDD9 pKa = 3.43LSKK12 pKa = 11.23AKK14 pKa = 10.45EE15 pKa = 4.33KK16 pKa = 10.69INKK19 pKa = 8.31TNVKK23 pKa = 9.73KK24 pKa = 10.84GQFAIANQALLDD36 pKa = 3.95MDD38 pKa = 4.84PYY40 pKa = 10.82IPLRR44 pKa = 11.84KK45 pKa = 9.89GPLRR49 pKa = 11.84SSGHH53 pKa = 3.95VTGGGSQIVYY63 pKa = 6.37NTPYY67 pKa = 11.0ARR69 pKa = 11.84AQFYY73 pKa = 10.35GGAYY77 pKa = 9.45NKK79 pKa = 10.02HH80 pKa = 6.2KK81 pKa = 10.73SFSFSKK87 pKa = 10.61YY88 pKa = 7.6STPGTGKK95 pKa = 10.37RR96 pKa = 11.84WDD98 pKa = 3.99LKK100 pKa = 10.99AKK102 pKa = 9.69PLHH105 pKa = 5.6ANKK108 pKa = 9.21WAEE111 pKa = 3.99IGLKK115 pKa = 10.79AMGIKK120 pKa = 10.01

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

11738

23

1299

195.6

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.952 ± 0.695

0.52 ± 0.094

6.781 ± 0.262

7.846 ± 0.397

3.961 ± 0.18

6.458 ± 0.544

1.397 ± 0.193

6.798 ± 0.263

8.741 ± 0.421

8.102 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.16

5.495 ± 0.212

2.862 ± 0.23

4.072 ± 0.208

4.422 ± 0.264

6.645 ± 0.398

5.308 ± 0.369

6.185 ± 0.25

1.278 ± 0.134

4.047 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski