Amino acid dipepetide frequency for Streptococcus phage PH15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.215AlaAla: 2.215 ± 0.97
0.341AlaCys: 0.341 ± 0.149
4.175AlaAsp: 4.175 ± 0.553
5.027AlaGlu: 5.027 ± 0.608
2.386AlaPhe: 2.386 ± 0.485
5.453AlaGly: 5.453 ± 1.89
0.852AlaHis: 0.852 ± 0.307
4.345AlaIle: 4.345 ± 0.433
6.56AlaLys: 6.56 ± 0.989
7.327AlaLeu: 7.327 ± 0.838
1.534AlaMet: 1.534 ± 0.423
3.578AlaAsn: 3.578 ± 0.549
1.96AlaPro: 1.96 ± 0.372
2.897AlaGln: 2.897 ± 0.696
2.897AlaArg: 2.897 ± 0.55
6.475AlaSer: 6.475 ± 1.521
3.919AlaThr: 3.919 ± 0.573
4.601AlaVal: 4.601 ± 0.744
0.596AlaTrp: 0.596 ± 0.219
2.386AlaTyr: 2.386 ± 0.545
0.0AlaXaa: 0.0 ± 0.0
Cys
0.256CysAla: 0.256 ± 0.14
0.0CysCys: 0.0 ± 0.0
0.767CysAsp: 0.767 ± 0.279
0.17CysGlu: 0.17 ± 0.109
0.256CysPhe: 0.256 ± 0.143
0.17CysGly: 0.17 ± 0.11
0.256CysHis: 0.256 ± 0.154
0.085CysIle: 0.085 ± 0.081
0.341CysLys: 0.341 ± 0.168
1.108CysLeu: 1.108 ± 0.305
0.0CysMet: 0.0 ± 0.0
0.256CysAsn: 0.256 ± 0.131
0.511CysPro: 0.511 ± 0.241
0.0CysGln: 0.0 ± 0.0
0.085CysArg: 0.085 ± 0.074
0.341CysSer: 0.341 ± 0.19
0.0CysThr: 0.0 ± 0.0
0.341CysVal: 0.341 ± 0.161
0.0CysTrp: 0.0 ± 0.0
0.256CysTyr: 0.256 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
3.578AspAla: 3.578 ± 0.646
0.511AspCys: 0.511 ± 0.239
4.345AspAsp: 4.345 ± 0.673
5.368AspGlu: 5.368 ± 0.539
4.004AspPhe: 4.004 ± 0.553
6.134AspGly: 6.134 ± 0.751
0.682AspHis: 0.682 ± 0.198
5.112AspIle: 5.112 ± 0.904
6.986AspLys: 6.986 ± 0.611
5.368AspLeu: 5.368 ± 0.563
1.363AspMet: 1.363 ± 0.334
3.493AspAsn: 3.493 ± 0.568
1.96AspPro: 1.96 ± 0.479
1.448AspGln: 1.448 ± 0.277
3.323AspArg: 3.323 ± 0.635
3.578AspSer: 3.578 ± 0.627
2.812AspThr: 2.812 ± 0.472
3.749AspVal: 3.749 ± 0.513
1.108AspTrp: 1.108 ± 0.419
2.897AspTyr: 2.897 ± 0.573
0.0AspXaa: 0.0 ± 0.0
Glu
5.453GluAla: 5.453 ± 0.638
0.341GluCys: 0.341 ± 0.167
4.43GluAsp: 4.43 ± 0.544
6.986GluGlu: 6.986 ± 0.925
3.408GluPhe: 3.408 ± 0.517
3.578GluGly: 3.578 ± 0.685
1.193GluHis: 1.193 ± 0.285
5.538GluIle: 5.538 ± 0.724
7.242GluLys: 7.242 ± 0.857
7.327GluLeu: 7.327 ± 0.855
2.726GluMet: 2.726 ± 0.513
3.749GluAsn: 3.749 ± 0.651
1.363GluPro: 1.363 ± 0.349
3.834GluGln: 3.834 ± 0.621
3.664GluArg: 3.664 ± 0.515
4.345GluSer: 4.345 ± 0.734
3.749GluThr: 3.749 ± 0.564
5.197GluVal: 5.197 ± 0.653
0.852GluTrp: 0.852 ± 0.26
3.493GluTyr: 3.493 ± 0.682
0.0GluXaa: 0.0 ± 0.0
Phe
2.13PheAla: 2.13 ± 0.402
0.426PheCys: 0.426 ± 0.252
4.175PheAsp: 4.175 ± 0.457
3.919PheGlu: 3.919 ± 0.67
1.108PhePhe: 1.108 ± 0.282
3.238PheGly: 3.238 ± 0.494
0.511PheHis: 0.511 ± 0.164
2.3PheIle: 2.3 ± 0.369
3.067PheLys: 3.067 ± 0.593
2.726PheLeu: 2.726 ± 0.373
0.767PheMet: 0.767 ± 0.203
2.13PheAsn: 2.13 ± 0.415
1.022PhePro: 1.022 ± 0.345
1.022PheGln: 1.022 ± 0.265
1.704PheArg: 1.704 ± 0.336
2.641PheSer: 2.641 ± 0.322
1.704PheThr: 1.704 ± 0.496
2.556PheVal: 2.556 ± 0.395
0.426PheTrp: 0.426 ± 0.181
2.045PheTyr: 2.045 ± 0.473
0.0PheXaa: 0.0 ± 0.0
Gly
4.771GlyAla: 4.771 ± 1.296
0.256GlyCys: 0.256 ± 0.139
4.516GlyAsp: 4.516 ± 0.599
4.09GlyGlu: 4.09 ± 0.494
4.345GlyPhe: 4.345 ± 1.118
4.09GlyGly: 4.09 ± 0.686
0.682GlyHis: 0.682 ± 0.233
6.475GlyIle: 6.475 ± 1.17
4.09GlyLys: 4.09 ± 0.484
5.197GlyLeu: 5.197 ± 0.842
1.278GlyMet: 1.278 ± 0.303
3.238GlyAsn: 3.238 ± 0.527
0.767GlyPro: 0.767 ± 0.251
3.238GlyGln: 3.238 ± 0.498
2.641GlyArg: 2.641 ± 0.467
3.578GlySer: 3.578 ± 0.727
3.919GlyThr: 3.919 ± 0.748
3.834GlyVal: 3.834 ± 0.502
0.937GlyTrp: 0.937 ± 0.257
2.641GlyTyr: 2.641 ± 0.64
0.0GlyXaa: 0.0 ± 0.0
His
0.682HisAla: 0.682 ± 0.247
0.0HisCys: 0.0 ± 0.0
1.022HisAsp: 1.022 ± 0.28
0.852HisGlu: 0.852 ± 0.262
0.511HisPhe: 0.511 ± 0.196
0.937HisGly: 0.937 ± 0.336
0.341HisHis: 0.341 ± 0.21
0.682HisIle: 0.682 ± 0.232
2.045HisLys: 2.045 ± 0.426
1.108HisLeu: 1.108 ± 0.323
0.085HisMet: 0.085 ± 0.097
0.256HisAsn: 0.256 ± 0.139
0.937HisPro: 0.937 ± 0.298
0.767HisGln: 0.767 ± 0.206
0.682HisArg: 0.682 ± 0.235
0.852HisSer: 0.852 ± 0.213
0.682HisThr: 0.682 ± 0.243
0.767HisVal: 0.767 ± 0.283
0.17HisTrp: 0.17 ± 0.143
0.596HisTyr: 0.596 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.686IleAla: 4.686 ± 0.759
0.256IleCys: 0.256 ± 0.157
5.623IleAsp: 5.623 ± 0.713
6.901IleGlu: 6.901 ± 0.724
1.874IlePhe: 1.874 ± 0.466
4.09IleGly: 4.09 ± 0.689
0.341IleHis: 0.341 ± 0.174
3.493IleIle: 3.493 ± 0.715
5.964IleLys: 5.964 ± 0.536
4.26IleLeu: 4.26 ± 0.826
1.278IleMet: 1.278 ± 0.352
4.516IleAsn: 4.516 ± 0.545
2.471IlePro: 2.471 ± 0.325
3.152IleGln: 3.152 ± 0.497
3.238IleArg: 3.238 ± 0.449
5.197IleSer: 5.197 ± 0.787
3.493IleThr: 3.493 ± 0.582
3.834IleVal: 3.834 ± 0.574
0.682IleTrp: 0.682 ± 0.224
2.641IleTyr: 2.641 ± 0.388
0.0IleXaa: 0.0 ± 0.0
Lys
7.327LysAla: 7.327 ± 1.051
0.426LysCys: 0.426 ± 0.166
5.879LysAsp: 5.879 ± 0.853
6.986LysGlu: 6.986 ± 0.683
2.812LysPhe: 2.812 ± 0.487
4.601LysGly: 4.601 ± 0.561
1.448LysHis: 1.448 ± 0.315
6.22LysIle: 6.22 ± 0.643
6.986LysLys: 6.986 ± 1.056
7.242LysLeu: 7.242 ± 0.723
1.789LysMet: 1.789 ± 0.406
5.112LysAsn: 5.112 ± 0.618
2.726LysPro: 2.726 ± 0.448
3.493LysGln: 3.493 ± 0.478
4.516LysArg: 4.516 ± 0.579
6.305LysSer: 6.305 ± 0.606
4.175LysThr: 4.175 ± 0.658
4.771LysVal: 4.771 ± 0.623
1.619LysTrp: 1.619 ± 0.299
2.897LysTyr: 2.897 ± 0.502
0.0LysXaa: 0.0 ± 0.0
Leu
6.986LeuAla: 6.986 ± 0.874
0.511LeuCys: 0.511 ± 0.204
5.879LeuAsp: 5.879 ± 0.663
6.475LeuGlu: 6.475 ± 0.7
2.641LeuPhe: 2.641 ± 0.507
6.305LeuGly: 6.305 ± 0.562
0.937LeuHis: 0.937 ± 0.353
4.856LeuIle: 4.856 ± 0.753
8.264LeuLys: 8.264 ± 0.87
4.686LeuLeu: 4.686 ± 0.718
1.448LeuMet: 1.448 ± 0.359
5.368LeuAsn: 5.368 ± 0.641
2.386LeuPro: 2.386 ± 0.4
3.152LeuGln: 3.152 ± 0.482
3.238LeuArg: 3.238 ± 0.499
6.049LeuSer: 6.049 ± 0.652
4.004LeuThr: 4.004 ± 0.583
4.856LeuVal: 4.856 ± 0.624
0.682LeuTrp: 0.682 ± 0.238
2.3LeuTyr: 2.3 ± 0.492
0.0LeuXaa: 0.0 ± 0.0
Met
1.96MetAla: 1.96 ± 0.323
0.0MetCys: 0.0 ± 0.0
1.278MetAsp: 1.278 ± 0.301
1.534MetGlu: 1.534 ± 0.425
0.596MetPhe: 0.596 ± 0.165
0.256MetGly: 0.256 ± 0.14
0.341MetHis: 0.341 ± 0.174
1.193MetIle: 1.193 ± 0.33
2.13MetLys: 2.13 ± 0.433
1.789MetLeu: 1.789 ± 0.444
0.426MetMet: 0.426 ± 0.198
1.108MetAsn: 1.108 ± 0.319
1.108MetPro: 1.108 ± 0.313
0.682MetGln: 0.682 ± 0.188
1.619MetArg: 1.619 ± 0.385
1.619MetSer: 1.619 ± 0.335
2.13MetThr: 2.13 ± 0.411
0.767MetVal: 0.767 ± 0.247
0.17MetTrp: 0.17 ± 0.095
0.596MetTyr: 0.596 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
4.26AsnAla: 4.26 ± 0.59
0.426AsnCys: 0.426 ± 0.216
3.323AsnAsp: 3.323 ± 0.586
4.09AsnGlu: 4.09 ± 0.54
2.045AsnPhe: 2.045 ± 0.434
4.601AsnGly: 4.601 ± 0.769
1.108AsnHis: 1.108 ± 0.312
2.982AsnIle: 2.982 ± 0.499
4.601AsnLys: 4.601 ± 0.753
3.834AsnLeu: 3.834 ± 0.545
1.193AsnMet: 1.193 ± 0.272
3.664AsnAsn: 3.664 ± 0.456
2.386AsnPro: 2.386 ± 0.435
2.215AsnGln: 2.215 ± 0.314
2.556AsnArg: 2.556 ± 0.569
3.408AsnSer: 3.408 ± 0.601
2.641AsnThr: 2.641 ± 0.424
3.238AsnVal: 3.238 ± 0.512
0.937AsnTrp: 0.937 ± 0.265
1.448AsnTyr: 1.448 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
1.874ProAla: 1.874 ± 0.486
0.085ProCys: 0.085 ± 0.089
1.789ProAsp: 1.789 ± 0.354
2.897ProGlu: 2.897 ± 0.352
1.022ProPhe: 1.022 ± 0.271
1.108ProGly: 1.108 ± 0.351
0.426ProHis: 0.426 ± 0.184
2.3ProIle: 2.3 ± 0.363
1.96ProLys: 1.96 ± 0.313
2.045ProLeu: 2.045 ± 0.462
0.767ProMet: 0.767 ± 0.286
1.619ProAsn: 1.619 ± 0.45
0.852ProPro: 0.852 ± 0.271
1.108ProGln: 1.108 ± 0.303
1.022ProArg: 1.022 ± 0.337
1.789ProSer: 1.789 ± 0.421
1.448ProThr: 1.448 ± 0.288
3.152ProVal: 3.152 ± 0.548
0.17ProTrp: 0.17 ± 0.115
1.193ProTyr: 1.193 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
3.152GlnAla: 3.152 ± 0.654
0.085GlnCys: 0.085 ± 0.088
1.874GlnAsp: 1.874 ± 0.451
2.812GlnGlu: 2.812 ± 0.682
1.96GlnPhe: 1.96 ± 0.357
2.812GlnGly: 2.812 ± 0.85
0.682GlnHis: 0.682 ± 0.304
2.3GlnIle: 2.3 ± 0.398
3.664GlnLys: 3.664 ± 0.717
3.919GlnLeu: 3.919 ± 0.478
1.022GlnMet: 1.022 ± 0.278
1.96GlnAsn: 1.96 ± 0.423
0.682GlnPro: 0.682 ± 0.262
1.789GlnGln: 1.789 ± 0.325
1.704GlnArg: 1.704 ± 0.39
2.641GlnSer: 2.641 ± 0.502
2.215GlnThr: 2.215 ± 0.411
2.897GlnVal: 2.897 ± 0.569
0.256GlnTrp: 0.256 ± 0.168
2.3GlnTyr: 2.3 ± 0.411
0.0GlnXaa: 0.0 ± 0.0
Arg
2.641ArgAla: 2.641 ± 0.573
0.17ArgCys: 0.17 ± 0.127
2.897ArgAsp: 2.897 ± 0.474
3.664ArgGlu: 3.664 ± 0.456
2.215ArgPhe: 2.215 ± 0.352
2.215ArgGly: 2.215 ± 0.372
0.596ArgHis: 0.596 ± 0.214
3.152ArgIle: 3.152 ± 0.64
4.345ArgLys: 4.345 ± 0.54
3.664ArgLeu: 3.664 ± 0.536
1.278ArgMet: 1.278 ± 0.377
3.408ArgAsn: 3.408 ± 0.59
0.767ArgPro: 0.767 ± 0.285
2.386ArgGln: 2.386 ± 0.359
1.96ArgArg: 1.96 ± 0.366
2.641ArgSer: 2.641 ± 0.434
2.215ArgThr: 2.215 ± 0.53
1.704ArgVal: 1.704 ± 0.344
0.596ArgTrp: 0.596 ± 0.223
1.704ArgTyr: 1.704 ± 0.404
0.0ArgXaa: 0.0 ± 0.0
Ser
5.708SerAla: 5.708 ± 1.462
0.426SerCys: 0.426 ± 0.179
4.004SerAsp: 4.004 ± 0.481
4.686SerGlu: 4.686 ± 0.706
2.471SerPhe: 2.471 ± 0.341
5.453SerGly: 5.453 ± 1.084
1.022SerHis: 1.022 ± 0.272
5.368SerIle: 5.368 ± 0.737
6.475SerLys: 6.475 ± 0.679
5.027SerLeu: 5.027 ± 0.714
1.363SerMet: 1.363 ± 0.28
2.812SerAsn: 2.812 ± 0.521
1.448SerPro: 1.448 ± 0.284
2.812SerGln: 2.812 ± 0.426
2.386SerArg: 2.386 ± 0.429
3.408SerSer: 3.408 ± 0.554
3.749SerThr: 3.749 ± 0.613
4.26SerVal: 4.26 ± 0.519
1.193SerTrp: 1.193 ± 0.374
2.386SerTyr: 2.386 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
4.345ThrAla: 4.345 ± 0.774
0.256ThrCys: 0.256 ± 0.197
3.152ThrAsp: 3.152 ± 0.478
4.601ThrGlu: 4.601 ± 0.825
2.045ThrPhe: 2.045 ± 0.349
3.664ThrGly: 3.664 ± 0.461
1.022ThrHis: 1.022 ± 0.282
4.43ThrIle: 4.43 ± 0.599
3.834ThrLys: 3.834 ± 0.535
4.26ThrLeu: 4.26 ± 0.563
0.767ThrMet: 0.767 ± 0.21
2.386ThrAsn: 2.386 ± 0.435
1.96ThrPro: 1.96 ± 0.424
1.789ThrGln: 1.789 ± 0.336
1.704ThrArg: 1.704 ± 0.388
3.067ThrSer: 3.067 ± 0.533
2.897ThrThr: 2.897 ± 0.647
3.578ThrVal: 3.578 ± 0.577
0.767ThrTrp: 0.767 ± 0.291
2.556ThrTyr: 2.556 ± 0.516
0.0ThrXaa: 0.0 ± 0.0
Val
3.919ValAla: 3.919 ± 0.653
0.341ValCys: 0.341 ± 0.173
5.027ValAsp: 5.027 ± 0.612
3.749ValGlu: 3.749 ± 0.512
1.704ValPhe: 1.704 ± 0.335
2.982ValGly: 2.982 ± 0.502
0.426ValHis: 0.426 ± 0.215
4.175ValIle: 4.175 ± 0.762
5.282ValLys: 5.282 ± 0.527
4.516ValLeu: 4.516 ± 0.788
1.448ValMet: 1.448 ± 0.364
3.408ValAsn: 3.408 ± 0.547
1.534ValPro: 1.534 ± 0.3
2.045ValGln: 2.045 ± 0.386
2.471ValArg: 2.471 ± 0.423
5.282ValSer: 5.282 ± 0.706
4.686ValThr: 4.686 ± 0.488
4.856ValVal: 4.856 ± 0.826
1.193ValTrp: 1.193 ± 0.421
2.812ValTyr: 2.812 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
1.278TrpAla: 1.278 ± 0.333
0.085TrpCys: 0.085 ± 0.084
1.193TrpAsp: 1.193 ± 0.401
1.108TrpGlu: 1.108 ± 0.324
0.596TrpPhe: 0.596 ± 0.231
0.767TrpGly: 0.767 ± 0.254
0.17TrpHis: 0.17 ± 0.123
0.426TrpIle: 0.426 ± 0.17
0.767TrpLys: 0.767 ± 0.308
1.363TrpLeu: 1.363 ± 0.262
0.17TrpMet: 0.17 ± 0.125
0.596TrpAsn: 0.596 ± 0.185
0.085TrpPro: 0.085 ± 0.078
0.937TrpGln: 0.937 ± 0.282
0.341TrpArg: 0.341 ± 0.172
0.852TrpSer: 0.852 ± 0.224
0.596TrpThr: 0.596 ± 0.216
0.767TrpVal: 0.767 ± 0.253
0.085TrpTrp: 0.085 ± 0.084
0.596TrpTyr: 0.596 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.3TyrAla: 2.3 ± 0.383
0.256TyrCys: 0.256 ± 0.175
2.471TyrAsp: 2.471 ± 0.383
2.556TyrGlu: 2.556 ± 0.366
1.619TyrPhe: 1.619 ± 0.349
2.13TyrGly: 2.13 ± 0.389
0.937TyrHis: 0.937 ± 0.265
2.556TyrIle: 2.556 ± 0.482
2.812TyrLys: 2.812 ± 0.566
4.26TyrLeu: 4.26 ± 0.812
0.511TyrMet: 0.511 ± 0.189
2.3TyrAsn: 2.3 ± 0.566
1.704TyrPro: 1.704 ± 0.338
1.96TyrGln: 1.96 ± 0.417
2.471TyrArg: 2.471 ± 0.442
2.386TyrSer: 2.386 ± 0.433
2.045TyrThr: 2.045 ± 0.364
2.13TyrVal: 2.13 ± 0.376
0.341TyrTrp: 0.341 ± 0.147
2.726TyrTyr: 2.726 ± 0.742
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (11738 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski