Siansivirga zeaxanthinifaciens CC-SAMT-1
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2744 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5WCM4|A0A0C5WCM4_9FLAO Uncharacterized protein OS=Siansivirga zeaxanthinifaciens CC-SAMT-1 OX=1454006 GN=AW14_03220 PE=4 SV=1
MM1 pKa = 7.11 GHH3 pKa = 5.59 NTGALTEE10 pKa = 4.21 QTAEE14 pKa = 4.4 VPASLPGAKK23 pKa = 9.56 RR24 pKa = 11.84 IGRR27 pKa = 11.84 EE28 pKa = 3.65 WLIKK32 pKa = 9.51 RR33 pKa = 11.84 TGDD36 pKa = 3.15 VGTVDD41 pKa = 4.93 LSFDD45 pKa = 3.67 VTGLTLSGSLFIDD58 pKa = 4.51 FKK60 pKa = 11.62 LLIDD64 pKa = 4.14 ADD66 pKa = 4.35 GDD68 pKa = 3.97 FSSGATIINASSFAGGKK85 pKa = 9.87 VSFTGVTLPDD95 pKa = 3.15 ANYY98 pKa = 8.03 ITFVTDD104 pKa = 3.18 VPLFLSSNLWLKK116 pKa = 10.58 GNQGVTNSGSNLAGWVDD133 pKa = 3.38 QTGINTFTVSGNPQTGVSKK152 pKa = 10.91 INFNNAIDD160 pKa = 3.86 FDD162 pKa = 4.35 GAGDD166 pKa = 4.05 YY167 pKa = 9.24 LTGNAAITFQNVYY180 pKa = 10.91 AVIKK184 pKa = 10.56 RR185 pKa = 11.84 EE186 pKa = 4.01 NTGDD190 pKa = 3.83 LTVLSASSGSANRR203 pKa = 11.84 GYY205 pKa = 11.33 VMKK208 pKa = 10.22 SNNMSTGNNDD218 pKa = 2.47 GSNYY222 pKa = 8.6 FRR224 pKa = 11.84 SVGTLGTDD232 pKa = 3.31 KK233 pKa = 11.18 ARR235 pKa = 11.84 IGHH238 pKa = 4.96 VEE240 pKa = 3.92 IVAGQPATNQKK251 pKa = 10.76 SYY253 pKa = 11.33 IDD255 pKa = 3.66 GQQFNTVSLVGSGNFVNYY273 pKa = 10.42 SDD275 pKa = 3.76 VPYY278 pKa = 10.69 VGRR281 pKa = 11.84 SQDD284 pKa = 3.39 NAQPDD289 pKa = 4.04 YY290 pKa = 11.18 FNGKK294 pKa = 7.51 IAEE297 pKa = 4.43 IIMYY301 pKa = 8.35 PAAHH305 pKa = 6.79 TSRR308 pKa = 11.84 EE309 pKa = 3.71 RR310 pKa = 11.84 SKK312 pKa = 10.39 IQSYY316 pKa = 9.87 LAIKK320 pKa = 10.02 YY321 pKa = 8.94 GISLDD326 pKa = 4.02 PSASAYY332 pKa = 10.44 LSSSEE337 pKa = 3.87 ALIWTDD343 pKa = 2.95 LTYY346 pKa = 10.71 WNDD349 pKa = 2.87 VFGIGKK355 pKa = 9.82 DD356 pKa = 3.57 DD357 pKa = 4.28 ASSLNQTQSNSINTGSGDD375 pKa = 3.48 GTGQSGKK382 pKa = 11.05 GNIIISNPSSLNNGDD397 pKa = 3.9 FLMIGHH403 pKa = 7.51 DD404 pKa = 3.55 NGALTEE410 pKa = 4.09 QATDD414 pKa = 3.6 LPTNYY419 pKa = 9.55 IGAARR424 pKa = 11.84 LARR427 pKa = 11.84 EE428 pKa = 4.44 WKK430 pKa = 9.79 VKK432 pKa = 8.94 HH433 pKa = 6.03 TGDD436 pKa = 4.05 VGTVDD441 pKa = 5.21 LKK443 pKa = 10.86 IDD445 pKa = 3.65 LTGLNLSGVPLNNFQLLLDD464 pKa = 4.23 ADD466 pKa = 4.53 GDD468 pKa = 4.15 FSSGATAVIPASFSSNILTFTSISLIDD495 pKa = 3.53 GTVFALVTKK504 pKa = 9.32 TAGPGVAGASLWLKK518 pKa = 9.56 TDD520 pKa = 3.82 DD521 pKa = 5.39 GITASGSNLTGWTDD535 pKa = 3.27 QTGTNTFSLNGTPTVVNNSVNFNPIVNFTNPTSPGVPVNGLIGDD579 pKa = 4.01 TEE581 pKa = 4.18 ISGVEE586 pKa = 3.56 AFGVFKK592 pKa = 11.27 YY593 pKa = 10.74 KK594 pKa = 10.72 DD595 pKa = 3.25 IVNQGTIVGNTTGDD609 pKa = 3.29 GGFFAGLLNVMKK621 pKa = 10.92 NRR623 pKa = 11.84 DD624 pKa = 3.7 TNGNDD629 pKa = 3.09 QTFANANLSTVFSISNIDD647 pKa = 3.84 LSPTLAQATVNGLAASVTQEE667 pKa = 3.96 KK668 pKa = 10.69 GVDD671 pKa = 3.99 FASVNLTPAIGGGANVGGLNGDD693 pKa = 3.68 LAEE696 pKa = 4.12 LVVYY700 pKa = 10.4 SKK702 pKa = 11.13 SLSALEE708 pKa = 4.0 KK709 pKa = 10.77 QKK711 pKa = 10.44 VQSYY715 pKa = 9.68 LAVKK719 pKa = 10.27 YY720 pKa = 10.44 GITLDD725 pKa = 3.7 QSISSYY731 pKa = 10.54 VSSNGTVIWDD741 pKa = 3.48 NTSYY745 pKa = 10.64 WNDD748 pKa = 2.68 VFGIGRR754 pKa = 11.84 EE755 pKa = 4.11 DD756 pKa = 3.76 ASGLNQTQSNSINSGSGDD774 pKa = 3.41 GTGQSGKK781 pKa = 10.96 GNIVISNPSSLEE793 pKa = 4.2 DD794 pKa = 4.15 GDD796 pKa = 4.36 FLLIGHH802 pKa = 7.28 DD803 pKa = 3.51 NGALTEE809 pKa = 4.13 QVTDD813 pKa = 4.7 LPADD817 pKa = 3.52 YY818 pKa = 10.82 AGRR821 pKa = 11.84 TRR823 pKa = 11.84 LTRR826 pKa = 11.84 EE827 pKa = 3.93 WKK829 pKa = 9.95 VKK831 pKa = 8.86 HH832 pKa = 6.03 TGDD835 pKa = 4.05 VGTVDD840 pKa = 5.21 LKK842 pKa = 10.86 IDD844 pKa = 3.65 LTGLNLSGVPLNNFQLLLDD863 pKa = 4.23 ADD865 pKa = 4.53 GDD867 pKa = 4.15 FSSGATAVIPASFSSNILTFTGISLTDD894 pKa = 3.31 GTVFALVTKK903 pKa = 9.32 TAGPGVAGASLWLKK917 pKa = 9.41 TDD919 pKa = 3.36 AGLTSSGSNLTGWTDD934 pKa = 3.28 QVGINTFNVNGSPEE948 pKa = 4.16 VVDD951 pKa = 4.08 NNINFNPSVYY961 pKa = 9.87 FANAPGSGLPEE972 pKa = 5.17 IGLIGNTEE980 pKa = 4.15 IIGVEE985 pKa = 3.54 AFGIFRR991 pKa = 11.84 HH992 pKa = 6.26 ADD994 pKa = 3.3 LVNQGTILGNSTGEE1008 pKa = 4.05 GGFFSSTLGIISTGDD1023 pKa = 3.32 SNGNDD1028 pKa = 2.99 QTFEE1032 pKa = 4.03 NPNVGLEE1039 pKa = 3.85 FSIVTIDD1046 pKa = 4.05 VSPTLADD1053 pKa = 3.56 AKK1055 pKa = 10.52 IDD1057 pKa = 3.65 GLAASVTQDD1066 pKa = 2.65 SGTDD1070 pKa = 3.72 FSSVSLIPVIGGGSLMTKK1088 pKa = 10.58 LNGEE1092 pKa = 4.22 LVEE1095 pKa = 4.77 LIVYY1099 pKa = 8.4 PSSLSAIDD1107 pKa = 3.4 KK1108 pKa = 10.67 RR1109 pKa = 11.84 KK1110 pKa = 8.01 VQSYY1114 pKa = 9.66 LAIKK1118 pKa = 10.06 YY1119 pKa = 8.97 GISLDD1124 pKa = 3.83 QSVTSYY1130 pKa = 9.68 MSSSGTVIWNNTTYY1144 pKa = 10.31 WNDD1147 pKa = 2.77 VFGIGKK1153 pKa = 9.94 DD1154 pKa = 3.57 DD1155 pKa = 3.73 VSGLNQSQSNSINTGSGDD1173 pKa = 3.52 GTGQTGKK1180 pKa = 11.13 GNIVISNPSSLEE1192 pKa = 4.21 DD1193 pKa = 4.02 GDD1195 pKa = 4.36 FLMIGHH1201 pKa = 7.47 DD1202 pKa = 3.37 NGALVEE1208 pKa = 4.14 QSTDD1212 pKa = 3.23 LPTSLSCFSRR1222 pKa = 11.84 IRR1224 pKa = 11.84 RR1225 pKa = 11.84 EE1226 pKa = 3.71 WKK1228 pKa = 9.8 VNHH1231 pKa = 6.35 TNDD1234 pKa = 3.12 VGTVDD1239 pKa = 5.01 LSFDD1243 pKa = 3.44 IKK1245 pKa = 10.96 GLTVGGTLKK1254 pKa = 10.75 EE1255 pKa = 4.11 DD1256 pKa = 3.24 FRR1258 pKa = 11.84 LVIDD1262 pKa = 3.94 QDD1264 pKa = 3.37 GDD1266 pKa = 4.17 GNFTTGTVTVITPNSLTSGVLLFQGVTLNSGAVFTFVTGPEE1307 pKa = 4.45 TISPTITCVSDD1318 pKa = 3.26 LTQPSDD1324 pKa = 3.68 AGNCSAVVNNIAPTASDD1341 pKa = 3.13 NCAVTTQTWTLTGATVKK1358 pKa = 10.8 NSATTGINDD1367 pKa = 3.81 ASGEE1371 pKa = 4.28 TFNLGVTTVTYY1382 pKa = 9.24 TVSDD1386 pKa = 3.39 AAGNTNSCSFDD1397 pKa = 3.25 VTITDD1402 pKa = 3.78 TEE1404 pKa = 4.54 LPTIATASNITVSSDD1419 pKa = 2.75 TGTCTYY1425 pKa = 10.92 ASSQLTPPTTDD1436 pKa = 3.77 DD1437 pKa = 3.51 NCGVASVVASPSSLAIGTNTVTWTVTDD1464 pKa = 3.57 NSGNTATTTQTVTVEE1479 pKa = 4.03 DD1480 pKa = 4.22 TEE1482 pKa = 4.78 LPTIATASNITVSSDD1497 pKa = 2.75 TGTCTYY1503 pKa = 10.92 ASSQLTPPTVNDD1515 pKa = 3.34 NCGVASVVASPSSLAIGTNTVTWTVTDD1542 pKa = 3.57 NSGNTATTTQTVTVEE1557 pKa = 4.22 DD1558 pKa = 3.87 NQLPTIATVGAVTVPADD1575 pKa = 3.88 AGVCTYY1581 pKa = 9.18 STSQLPPPSTDD1592 pKa = 3.54 DD1593 pKa = 3.51 NCGVASVVVSPSSLALGAHH1612 pKa = 5.18 TVTWTVTDD1620 pKa = 3.57 NSGNTATTTEE1630 pKa = 4.4 TVTVVDD1636 pKa = 4.19 TEE1638 pKa = 4.57 LPTIATVGAVTVPADD1653 pKa = 3.88 AGVCTYY1659 pKa = 9.18 STSQLPPPSTDD1670 pKa = 3.54 DD1671 pKa = 3.51 NCGVASVVGIPSTLNAGSNTVTWIVVDD1698 pKa = 4.9 DD1699 pKa = 4.32 SGNQNRR1705 pKa = 11.84 SIQTVIVVEE1714 pKa = 4.24 NEE1716 pKa = 3.71 LPTISNLGPITVNSDD1731 pKa = 3.29 PGVCTYY1737 pKa = 11.2 ASSQLTPPTTNDD1749 pKa = 2.9 NCGVASVVASPSSLVLGDD1767 pKa = 5.01 NLVTWTVTDD1776 pKa = 3.43 NAGNVANSLQTVTVVDD1792 pKa = 3.76 NEE1794 pKa = 4.16 RR1795 pKa = 11.84 PTIVIAGPVTVSSDD1809 pKa = 3.43 PGVCTYY1815 pKa = 11.2 ASSQLTPPTTNDD1827 pKa = 2.9 NCGVASVVVSPSSLAIGNNTVTWTVTDD1854 pKa = 4.05 DD1855 pKa = 3.75 SGNTATATQIVTVEE1869 pKa = 4.12 DD1870 pKa = 4.04 TEE1872 pKa = 4.46 NPTIASVGSVTVNVDD1887 pKa = 3.5 AGLCTYY1893 pKa = 7.63 DD1894 pKa = 3.82 TSQLPPPAADD1904 pKa = 4.17 DD1905 pKa = 3.72 NCGVLFVISDD1915 pKa = 3.65 KK1916 pKa = 9.14 TTLNVGVNTLTWYY1929 pKa = 10.69 AFDD1932 pKa = 4.23 FEE1934 pKa = 6.33 LNFSSTTQTVIVVEE1948 pKa = 4.39 NEE1950 pKa = 3.71 APTIATLGPITVSSDD1965 pKa = 3.31 LGVCTYY1971 pKa = 10.96 DD1972 pKa = 4.13 SSQLPPPATSDD1983 pKa = 3.34 NCGVASVVASPSTLVLGPNTVTWTVTDD2010 pKa = 3.55 NAGNIANSIQTVTVEE2025 pKa = 4.49 DD2026 pKa = 3.85 NQAPTIATVSAVTVSADD2043 pKa = 4.19 AGVCTYY2049 pKa = 8.07 NTSQLPPPATSDD2061 pKa = 3.34 NCGVASVVASPSTLVLGSNTVTWTVTDD2088 pKa = 3.33 NAGNIKK2094 pKa = 7.79 TTIQGVTVQDD2104 pKa = 3.69 NEE2106 pKa = 4.35 APTFTSVSAITVSADD2121 pKa = 3.33 AGVCTYY2127 pKa = 11.08 ASSQLTPPSVTDD2139 pKa = 3.26 NCGVAGILRR2148 pKa = 11.84 NPGVLSVGINTVTWTAIDD2166 pKa = 3.7 DD2167 pKa = 4.03 SGNQKK2172 pKa = 10.75 SIIQTVTVEE2181 pKa = 4.23 DD2182 pKa = 4.26 NEE2184 pKa = 4.69 DD2185 pKa = 3.4 PTIASASNITVSSDD2199 pKa = 3.27 AGTCTYY2205 pKa = 11.02 ASSQLTPPTVNDD2217 pKa = 3.34 NCGVASVVASPSTLVLGPNTVTWTVTDD2244 pKa = 3.63 NAGNTASTTQTVTVEE2259 pKa = 4.21 DD2260 pKa = 4.36 NEE2262 pKa = 4.72 DD2263 pKa = 3.42 PTIAAASNITVSSDD2277 pKa = 3.27 AGTCTYY2283 pKa = 11.35 ASAQLTPPTVNDD2295 pKa = 3.34 NCGVASVVASPSTIVLGPNTVTWTVTDD2322 pKa = 3.63 NAGNTASTTQTVTVEE2337 pKa = 4.21 DD2338 pKa = 4.36 NEE2340 pKa = 4.69 DD2341 pKa = 3.4 PTIASASNITVSSDD2355 pKa = 3.27 AGTCTYY2361 pKa = 11.02 ASSQLTPPTVNDD2373 pKa = 3.34 NCGVASVVASPSSLALGANTVTWTVTDD2400 pKa = 3.63 NAGNTASTTQTVTVEE2415 pKa = 4.21 DD2416 pKa = 4.36 NEE2418 pKa = 4.72 DD2419 pKa = 3.42 PTIAAASNITVSSDD2433 pKa = 3.27 AGTCTYY2439 pKa = 11.35 ASAQLTPPTVNDD2451 pKa = 3.34 NCGVASVVASPSSLALGANTVTWTVTDD2478 pKa = 3.63 NAGNTASTTQTVTVEE2493 pKa = 4.2 DD2494 pKa = 3.84 NQLPTVSCPANQTVVADD2511 pKa = 3.31 VSGFYY2516 pKa = 10.01 TLPDD2520 pKa = 3.58 YY2521 pKa = 11.03 FGTGAGSATDD2531 pKa = 3.63 TCSGTIAVYY2540 pKa = 10.21 SQSPTVGTQLGIGVYY2555 pKa = 9.79 TITLTAEE2562 pKa = 4.26 DD2563 pKa = 3.76 ASGNIGSCDD2572 pKa = 3.53 FEE2574 pKa = 4.66 LTVSAPLGLEE2584 pKa = 3.91 DD2585 pKa = 5.21 LNRR2588 pKa = 11.84 DD2589 pKa = 2.81 IKK2591 pKa = 10.14 TIQLYY2596 pKa = 9.65 PNPSSNLVYY2605 pKa = 10.52 LSNPSRR2611 pKa = 11.84 INLQEE2616 pKa = 3.62 VSIYY2620 pKa = 10.95 DD2621 pKa = 3.32 VTGRR2625 pKa = 11.84 LIEE2628 pKa = 4.21 TVKK2631 pKa = 10.83 LKK2633 pKa = 10.85 SEE2635 pKa = 4.24 EE2636 pKa = 4.03 KK2637 pKa = 10.58 QEE2639 pKa = 4.28 VIDD2642 pKa = 3.81 MSHH2645 pKa = 7.08 WDD2647 pKa = 3.05 SAMYY2651 pKa = 10.61 LFVIKK2656 pKa = 10.44 SDD2658 pKa = 3.49 LGQVTKK2664 pKa = 10.9 SVVKK2668 pKa = 10.13 EE2669 pKa = 3.84
Molecular weight: 274.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.674
IPC2_protein 3.745
IPC_protein 3.808
Toseland 3.567
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.757
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.923
Patrickios 1.443
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.83
Protein with the highest isoelectric point:
>tr|A0A0C5VWL3|A0A0C5VWL3_9FLAO Membrane protein OS=Siansivirga zeaxanthinifaciens CC-SAMT-1 OX=1454006 GN=AW14_07575 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 7.97 KK42 pKa = 10.6 LSVSSEE48 pKa = 4.04 SRR50 pKa = 11.84 HH51 pKa = 5.63 KK52 pKa = 10.69 KK53 pKa = 9.55
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2744
0
2744
966228
49
4610
352.1
39.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.409 ± 0.049
0.77 ± 0.017
5.583 ± 0.038
6.275 ± 0.052
5.257 ± 0.039
6.323 ± 0.046
1.75 ± 0.023
8.132 ± 0.045
7.891 ± 0.07
9.228 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.039 ± 0.025
6.723 ± 0.05
3.378 ± 0.026
3.269 ± 0.022
3.16 ± 0.029
6.462 ± 0.037
6.033 ± 0.065
6.213 ± 0.043
1.048 ± 0.016
4.059 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here