Pseudomonas agarici

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0X1T0X6|A0A0X1T0X6_PSEAA FixH family protein OS=Pseudomonas agarici OX=46677 GN=AWM79_10475 PE=4 SV=1
MM1 pKa = 7.3PALYY5 pKa = 11.13VMIPAALLIVAIAVYY20 pKa = 10.34IFFWAVDD27 pKa = 3.27SGQYY31 pKa = 10.95DD32 pKa = 4.11DD33 pKa = 5.98LDD35 pKa = 4.98GPAHH39 pKa = 6.91SILFDD44 pKa = 4.13DD45 pKa = 5.22QDD47 pKa = 4.38PNHH50 pKa = 5.87QAAIEE55 pKa = 4.09EE56 pKa = 4.6ANGTPRR62 pKa = 11.84DD63 pKa = 4.01HH64 pKa = 7.38DD65 pKa = 3.87QQAPPHH71 pKa = 5.84AA72 pKa = 5.16

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X1T2U0|A0A0X1T2U0_PSEAA Isocitrate dehydrogenase [NADP] OS=Pseudomonas agarici OX=46677 GN=AWM79_14340 PE=3 SV=1
MM1 pKa = 7.76AKK3 pKa = 10.15PGFRR7 pKa = 11.84LAVFATLLALIVVLLGAYY25 pKa = 7.48TRR27 pKa = 11.84LTHH30 pKa = 7.16AGLGCPDD37 pKa = 3.03WPGCYY42 pKa = 10.27GFISVPKK49 pKa = 10.4SEE51 pKa = 4.52AQLAHH56 pKa = 6.93AEE58 pKa = 4.11LHH60 pKa = 6.64FPDD63 pKa = 4.62TPVEE67 pKa = 3.92AHH69 pKa = 6.58KK70 pKa = 10.61GWNEE74 pKa = 3.63MIHH77 pKa = 6.8RR78 pKa = 11.84YY79 pKa = 8.38FAGTLGLLIVLLASRR94 pKa = 11.84SWMEE98 pKa = 3.89RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84EE102 pKa = 4.02GQPLKK107 pKa = 11.12LPLLLLAVVFAQAAFGMWTVTLKK130 pKa = 10.75LWPQVVTGHH139 pKa = 6.85LLGGFATLSLLFLLALRR156 pKa = 11.84LSGVLPALAVPRR168 pKa = 11.84RR169 pKa = 11.84LQRR172 pKa = 11.84WASAGLLLVILQIALGGWVSSNYY195 pKa = 10.3AAVACIDD202 pKa = 4.28LPTCHH207 pKa = 6.0GQWWPSADD215 pKa = 3.48FANGFHH221 pKa = 6.71LTQHH225 pKa = 6.83IGPNYY230 pKa = 10.4LGGQLDD236 pKa = 3.67SDD238 pKa = 3.86ARR240 pKa = 11.84TAIHH244 pKa = 5.82LTHH247 pKa = 7.0RR248 pKa = 11.84VGALLVTLVLLGLAWQLRR266 pKa = 11.84VVGMSRR272 pKa = 11.84LAGLLLIALVAQISLGLSNVYY293 pKa = 9.5FHH295 pKa = 7.24LPLPVAVAHH304 pKa = 6.02NAGGAALLLTLVLINYY320 pKa = 7.83HH321 pKa = 6.4ARR323 pKa = 11.84TALVRR328 pKa = 11.84VRR330 pKa = 11.84QRR332 pKa = 11.84TPVRR336 pKa = 11.84WRR338 pKa = 11.84LSPRR342 pKa = 11.84KK343 pKa = 9.14SVHH346 pKa = 5.78GPITIKK352 pKa = 11.17GEE354 pKa = 4.27MPWRR358 pKa = 11.84LL359 pKa = 3.22

Molecular weight: 39.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4626

0

4626

1550874

24

5589

335.3

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.592 ± 0.041

1.001 ± 0.012

5.352 ± 0.026

5.672 ± 0.038

3.667 ± 0.02

7.787 ± 0.038

2.318 ± 0.016

5.017 ± 0.029

3.56 ± 0.035

11.894 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.018

3.107 ± 0.025

4.84 ± 0.025

4.614 ± 0.028

6.48 ± 0.038

6.007 ± 0.026

4.872 ± 0.025

7.001 ± 0.03

1.402 ± 0.013

2.572 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski