Acidibacillus ferrooxidans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillales incertae sedis; Acidibacillus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A162TKE9|A0A162TKE9_9BACL Fe-S cluster assembly protein SufB OS=Acidibacillus ferrooxidans OX=1765683 GN=AYJ22_01715 PE=3 SV=1
MM1 pKa = 7.48AAFVSVGAFQVASVANDD18 pKa = 2.89VGFFYY23 pKa = 10.59GQNVQNAWDD32 pKa = 3.64SHH34 pKa = 7.11APLQMGAGFTMGNYY48 pKa = 10.73DD49 pKa = 3.94LMNNAGIVSNTPAAVLQPINDD70 pKa = 3.52QDD72 pKa = 3.85VKK74 pKa = 11.71DD75 pKa = 4.06NFSPSWQGPP84 pKa = 3.36

Molecular weight:
8.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1V4EVF0|A0A1V4EVF0_9BACL Flagellar hook-associated protein 1 OS=Acidibacillus ferrooxidans OX=1765683 GN=flgK PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 8.58PTYY5 pKa = 9.95NPNVRR10 pKa = 11.84KK11 pKa = 9.7RR12 pKa = 11.84KK13 pKa = 8.69KK14 pKa = 8.48VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.61NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.29GRR39 pKa = 11.84KK40 pKa = 8.93VISAA44 pKa = 4.05

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2881

0

2881

885484

25

2363

307.4

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.632 ± 0.05

0.851 ± 0.015

4.906 ± 0.043

5.946 ± 0.057

3.998 ± 0.034

7.413 ± 0.047

2.554 ± 0.022

5.958 ± 0.041

3.763 ± 0.035

10.525 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.023

2.99 ± 0.034

4.262 ± 0.03

3.884 ± 0.03

6.505 ± 0.05

6.215 ± 0.043

5.844 ± 0.039

7.917 ± 0.04

1.165 ± 0.02

2.899 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski