Acidibacillus ferrooxidans
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162TKE9|A0A162TKE9_9BACL Fe-S cluster assembly protein SufB OS=Acidibacillus ferrooxidans OX=1765683 GN=AYJ22_01715 PE=3 SV=1
MM1 pKa = 7.48 AAFVSVGAFQVASVANDD18 pKa = 2.89 VGFFYY23 pKa = 10.59 GQNVQNAWDD32 pKa = 3.64 SHH34 pKa = 7.11 APLQMGAGFTMGNYY48 pKa = 10.73 DD49 pKa = 3.94 LMNNAGIVSNTPAAVLQPINDD70 pKa = 3.52 QDD72 pKa = 3.85 VKK74 pKa = 11.71 DD75 pKa = 4.06 NFSPSWQGPP84 pKa = 3.36
Molecular weight: 8.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.757
IPC_protein 3.617
Toseland 3.414
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.732
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.579
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.541
EMBOSS 3.732
Sillero 3.77
Patrickios 1.914
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A1V4EVF0|A0A1V4EVF0_9BACL Flagellar hook-associated protein 1 OS=Acidibacillus ferrooxidans OX=1765683 GN=flgK PE=3 SV=1
MM1 pKa = 7.52 KK2 pKa = 8.58 PTYY5 pKa = 9.95 NPNVRR10 pKa = 11.84 KK11 pKa = 9.7 RR12 pKa = 11.84 KK13 pKa = 8.69 KK14 pKa = 8.48 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTKK25 pKa = 9.61 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.29 GRR39 pKa = 11.84 KK40 pKa = 8.93 VISAA44 pKa = 4.05
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2881
0
2881
885484
25
2363
307.4
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.632 ± 0.05
0.851 ± 0.015
4.906 ± 0.043
5.946 ± 0.057
3.998 ± 0.034
7.413 ± 0.047
2.554 ± 0.022
5.958 ± 0.041
3.763 ± 0.035
10.525 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.023
2.99 ± 0.034
4.262 ± 0.03
3.884 ± 0.03
6.505 ± 0.05
6.215 ± 0.043
5.844 ± 0.039
7.917 ± 0.04
1.165 ± 0.02
2.899 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here