Methylobacterium gossipiicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4215 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2UQZ4|A0A1I2UQZ4_9RHIZ Uncharacterized protein OS=Methylobacterium gossipiicola OX=582675 GN=SAMN05192565_11113 PE=4 SV=1
MM1 pKa = 7.26ATDD4 pKa = 4.55LSLANSYY11 pKa = 10.46AHH13 pKa = 6.57SLSLSLMVPVTVFRR27 pKa = 11.84SDD29 pKa = 2.44VGYY32 pKa = 10.99AVVTSDD38 pKa = 4.4EE39 pKa = 4.47FEE41 pKa = 4.67GDD43 pKa = 3.25PASVVTEE50 pKa = 3.97FDD52 pKa = 3.32PYY54 pKa = 10.74EE55 pKa = 3.92AA56 pKa = 5.44

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2R2Y9|A0A1I2R2Y9_9RHIZ LemA protein OS=Methylobacterium gossipiicola OX=582675 GN=SAMN05192565_10230 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.15VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.62GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.66LSAA44 pKa = 3.99

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4215

0

4215

1312095

24

3248

311.3

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.131 ± 0.06

0.797 ± 0.011

5.686 ± 0.03

5.357 ± 0.039

3.385 ± 0.025

9.043 ± 0.048

1.951 ± 0.02

4.4 ± 0.026

2.651 ± 0.033

10.405 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.02

2.166 ± 0.022

5.798 ± 0.037

2.715 ± 0.023

8.042 ± 0.045

4.832 ± 0.026

5.709 ± 0.043

7.662 ± 0.029

1.225 ± 0.016

2.002 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski