Amino acid dipepetide frequency for Methylobacterium gossipiicola

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.909AlaAla: 20.909 ± 0.175	1.33AlaCys: 1.33 ± 0.042	7.404AlaAsp: 7.404 ± 0.083	8.507AlaGlu: 8.507 ± 0.1	4.75AlaPhe: 4.75 ± 0.074	12.341AlaGly: 12.341 ± 0.13	2.464AlaHis: 2.464 ± 0.046	6.021AlaIle: 6.021 ± 0.07	3.873AlaLys: 3.873 ± 0.064	15.667AlaLeu: 15.667 ± 0.149	3.446AlaMet: 3.446 ± 0.061	2.736AlaAsn: 2.736 ± 0.054	7.355AlaPro: 7.355 ± 0.102	4.135AlaGln: 4.135 ± 0.063	11.443AlaArg: 11.443 ± 0.113	6.554AlaSer: 6.554 ± 0.075	7.358AlaThr: 7.358 ± 0.084	10.383AlaVal: 10.383 ± 0.103	1.847AlaTrp: 1.847 ± 0.04	2.781AlaTyr: 2.781 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.011CysAla: 1.011 ± 0.03	0.088CysCys: 0.088 ± 0.009	0.479CysAsp: 0.479 ± 0.019	0.428CysGlu: 0.428 ± 0.02	0.262CysPhe: 0.262 ± 0.014	0.897CysGly: 0.897 ± 0.031	0.206CysHis: 0.206 ± 0.015	0.308CysIle: 0.308 ± 0.015	0.155CysLys: 0.155 ± 0.011	0.905CysLeu: 0.905 ± 0.027	0.137CysMet: 0.137 ± 0.011	0.176CysAsn: 0.176 ± 0.011	0.487CysPro: 0.487 ± 0.023	0.173CysGln: 0.173 ± 0.012	0.681CysArg: 0.681 ± 0.025	0.327CysSer: 0.327 ± 0.018	0.425CysThr: 0.425 ± 0.016	0.595CysVal: 0.595 ± 0.019	0.092CysTrp: 0.092 ± 0.007	0.137CysTyr: 0.137 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.019AspAla: 8.019 ± 0.089	0.395AspCys: 0.395 ± 0.019	2.952AspAsp: 2.952 ± 0.054	3.098AspGlu: 3.098 ± 0.059	1.875AspPhe: 1.875 ± 0.043	5.76AspGly: 5.76 ± 0.08	1.172AspHis: 1.172 ± 0.031	2.354AspIle: 2.354 ± 0.046	1.324AspLys: 1.324 ± 0.034	6.856AspLeu: 6.856 ± 0.085	0.964AspMet: 0.964 ± 0.027	0.974AspAsn: 0.974 ± 0.031	4.045AspPro: 4.045 ± 0.063	1.295AspGln: 1.295 ± 0.032	4.992AspArg: 4.992 ± 0.068	1.848AspSer: 1.848 ± 0.035	2.733AspThr: 2.733 ± 0.05	4.095AspVal: 4.095 ± 0.059	0.866AspTrp: 0.866 ± 0.031	1.24AspTyr: 1.24 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	9.352GluAla: 9.352 ± 0.111	0.307GluCys: 0.307 ± 0.016	2.423GluAsp: 2.423 ± 0.051	2.631GluGlu: 2.631 ± 0.055	1.366GluPhe: 1.366 ± 0.031	4.557GluGly: 4.557 ± 0.062	1.072GluHis: 1.072 ± 0.03	3.046GluIle: 3.046 ± 0.051	1.559GluLys: 1.559 ± 0.039	4.01GluLeu: 4.01 ± 0.055	1.198GluMet: 1.198 ± 0.035	1.144GluAsn: 1.144 ± 0.029	3.014GluPro: 3.014 ± 0.058	1.492GluGln: 1.492 ± 0.039	5.428GluArg: 5.428 ± 0.078	2.177GluSer: 2.177 ± 0.042	3.908GluThr: 3.908 ± 0.059	3.633GluVal: 3.633 ± 0.052	0.514GluTrp: 0.514 ± 0.021	0.735GluTyr: 0.735 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.627PheAla: 4.627 ± 0.073	0.331PheCys: 0.331 ± 0.015	2.394PheAsp: 2.394 ± 0.05	1.939PheGlu: 1.939 ± 0.043	1.167PhePhe: 1.167 ± 0.031	3.533PheGly: 3.533 ± 0.063	0.659PheHis: 0.659 ± 0.025	1.174PheIle: 1.174 ± 0.031	0.854PheLys: 0.854 ± 0.025	3.216PheLeu: 3.216 ± 0.055	0.588PheMet: 0.588 ± 0.023	0.783PheAsn: 0.783 ± 0.024	1.528PhePro: 1.528 ± 0.037	0.87PheGln: 0.87 ± 0.027	2.317PheArg: 2.317 ± 0.043	1.897PheSer: 1.897 ± 0.043	2.052PheThr: 2.052 ± 0.039	2.724PheVal: 2.724 ± 0.043	0.433PheTrp: 0.433 ± 0.023	0.764PheTyr: 0.764 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	11.096GlyAla: 11.096 ± 0.118	0.807GlyCys: 0.807 ± 0.025	4.609GlyAsp: 4.609 ± 0.07	4.62GlyGlu: 4.62 ± 0.06	3.674GlyPhe: 3.674 ± 0.059	7.884GlyGly: 7.884 ± 0.13	1.927GlyHis: 1.927 ± 0.045	4.302GlyIle: 4.302 ± 0.071	2.475GlyLys: 2.475 ± 0.051	10.766GlyLeu: 10.766 ± 0.117	1.851GlyMet: 1.851 ± 0.04	1.812GlyAsn: 1.812 ± 0.048	4.465GlyPro: 4.465 ± 0.064	2.831GlyGln: 2.831 ± 0.052	7.663GlyArg: 7.663 ± 0.081	4.564GlySer: 4.564 ± 0.072	5.431GlyThr: 5.431 ± 0.117	6.188GlyVal: 6.188 ± 0.073	1.274GlyTrp: 1.274 ± 0.036	2.193GlyTyr: 2.193 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.655HisAla: 2.655 ± 0.055	0.196HisCys: 0.196 ± 0.013	1.227HisAsp: 1.227 ± 0.034	0.932HisGlu: 0.932 ± 0.028	0.697HisPhe: 0.697 ± 0.021	2.02HisGly: 2.02 ± 0.042	0.565HisHis: 0.565 ± 0.023	0.69HisIle: 0.69 ± 0.021	0.348HisLys: 0.348 ± 0.017	2.184HisLeu: 2.184 ± 0.043	0.356HisMet: 0.356 ± 0.017	0.38HisAsn: 0.38 ± 0.019	1.451HisPro: 1.451 ± 0.035	0.452HisGln: 0.452 ± 0.02	1.64HisArg: 1.64 ± 0.038	0.676HisSer: 0.676 ± 0.022	0.836HisThr: 0.836 ± 0.027	1.427HisVal: 1.427 ± 0.034	0.305HisTrp: 0.305 ± 0.018	0.476HisTyr: 0.476 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.718IleAla: 6.718 ± 0.086	0.377IleCys: 0.377 ± 0.019	3.063IleAsp: 3.063 ± 0.041	3.077IleGlu: 3.077 ± 0.047	1.139IlePhe: 1.139 ± 0.036	4.5IleGly: 4.5 ± 0.068	0.79IleHis: 0.79 ± 0.027	1.399IleIle: 1.399 ± 0.039	1.078IleLys: 1.078 ± 0.033	4.332IleLeu: 4.332 ± 0.061	0.634IleMet: 0.634 ± 0.023	0.972IleAsn: 0.972 ± 0.033	2.216IlePro: 2.216 ± 0.041	1.115IleGln: 1.115 ± 0.028	3.228IleArg: 3.228 ± 0.055	1.808IleSer: 1.808 ± 0.045	2.361IleThr: 2.361 ± 0.047	3.938IleVal: 3.938 ± 0.059	0.437IleTrp: 0.437 ± 0.018	0.823IleTyr: 0.823 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.042LysAla: 4.042 ± 0.072	0.114LysCys: 0.114 ± 0.009	1.418LysAsp: 1.418 ± 0.037	1.123LysGlu: 1.123 ± 0.036	0.675LysPhe: 0.675 ± 0.023	2.345LysGly: 2.345 ± 0.047	0.404LysHis: 0.404 ± 0.018	1.231LysIle: 1.231 ± 0.032	0.903LysLys: 0.903 ± 0.036	2.467LysLeu: 2.467 ± 0.048	0.489LysMet: 0.489 ± 0.018	0.648LysAsn: 0.648 ± 0.027	2.044LysPro: 2.044 ± 0.042	0.733LysGln: 0.733 ± 0.027	2.114LysArg: 2.114 ± 0.041	1.408LysSer: 1.408 ± 0.037	1.649LysThr: 1.649 ± 0.042	1.998LysVal: 1.998 ± 0.042	0.257LysTrp: 0.257 ± 0.015	0.449LysTyr: 0.449 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	16.164LeuAla: 16.164 ± 0.142	0.944LeuCys: 0.944 ± 0.027	6.819LeuAsp: 6.819 ± 0.089	4.495LeuGlu: 4.495 ± 0.063	3.44LeuPhe: 3.44 ± 0.055	9.247LeuGly: 9.247 ± 0.108	1.95LeuHis: 1.95 ± 0.044	4.187LeuIle: 4.187 ± 0.066	3.093LeuLys: 3.093 ± 0.055	9.131LeuLeu: 9.131 ± 0.113	2.059LeuMet: 2.059 ± 0.038	2.264LeuAsn: 2.264 ± 0.043	5.945LeuPro: 5.945 ± 0.072	2.376LeuGln: 2.376 ± 0.045	7.872LeuArg: 7.872 ± 0.093	6.059LeuSer: 6.059 ± 0.081	6.208LeuThr: 6.208 ± 0.082	8.645LeuVal: 8.645 ± 0.098	1.104LeuTrp: 1.104 ± 0.03	2.053LeuTyr: 2.053 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.814MetAla: 2.814 ± 0.049	0.118MetCys: 0.118 ± 0.01	0.872MetAsp: 0.872 ± 0.026	0.904MetGlu: 0.904 ± 0.027	0.486MetPhe: 0.486 ± 0.019	1.561MetGly: 1.561 ± 0.038	0.341MetHis: 0.341 ± 0.017	1.026MetIle: 1.026 ± 0.027	0.684MetLys: 0.684 ± 0.022	1.985MetLeu: 1.985 ± 0.044	0.485MetMet: 0.485 ± 0.019	0.6MetAsn: 0.6 ± 0.021	1.456MetPro: 1.456 ± 0.037	0.631MetGln: 0.631 ± 0.019	1.785MetArg: 1.785 ± 0.036	1.333MetSer: 1.333 ± 0.03	1.622MetThr: 1.622 ± 0.038	1.362MetVal: 1.362 ± 0.041	0.135MetTrp: 0.135 ± 0.01	0.207MetTyr: 0.207 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.97AsnAla: 2.97 ± 0.049	0.181AsnCys: 0.181 ± 0.011	1.154AsnAsp: 1.154 ± 0.032	0.958AsnGlu: 0.958 ± 0.026	0.758AsnPhe: 0.758 ± 0.024	2.079AsnGly: 2.079 ± 0.047	0.428AsnHis: 0.428 ± 0.019	0.919AsnIle: 0.919 ± 0.03	0.477AsnLys: 0.477 ± 0.023	2.344AsnLeu: 2.344 ± 0.048	0.423AsnMet: 0.423 ± 0.02	0.511AsnAsn: 0.511 ± 0.024	1.67AsnPro: 1.67 ± 0.035	0.603AsnGln: 0.603 ± 0.024	1.79AsnArg: 1.79 ± 0.043	0.872AsnSer: 0.872 ± 0.032	1.145AsnThr: 1.145 ± 0.035	1.597AsnVal: 1.597 ± 0.038	0.293AsnTrp: 0.293 ± 0.015	0.492AsnTyr: 0.492 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	7.703ProAla: 7.703 ± 0.103	0.369ProCys: 0.369 ± 0.019	4.204ProAsp: 4.204 ± 0.064	4.101ProGlu: 4.101 ± 0.065	1.988ProPhe: 1.988 ± 0.038	5.457ProGly: 5.457 ± 0.072	1.122ProHis: 1.122 ± 0.028	2.267ProIle: 2.267 ± 0.038	1.696ProLys: 1.696 ± 0.04	5.164ProLeu: 5.164 ± 0.066	1.244ProMet: 1.244 ± 0.034	1.348ProAsn: 1.348 ± 0.037	3.355ProPro: 3.355 ± 0.066	1.499ProGln: 1.499 ± 0.031	3.821ProArg: 3.821 ± 0.065	2.901ProSer: 2.901 ± 0.057	2.943ProThr: 2.943 ± 0.044	4.877ProVal: 4.877 ± 0.064	0.787ProTrp: 0.787 ± 0.026	1.138ProTyr: 1.138 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.532GlnAla: 4.532 ± 0.063	0.172GlnCys: 0.172 ± 0.01	1.323GlnAsp: 1.323 ± 0.035	1.152GlnGlu: 1.152 ± 0.034	0.835GlnPhe: 0.835 ± 0.025	2.427GlnGly: 2.427 ± 0.048	0.505GlnHis: 0.505 ± 0.021	1.514GlnIle: 1.514 ± 0.039	0.774GlnLys: 0.774 ± 0.028	2.126GlnLeu: 2.126 ± 0.048	0.53GlnMet: 0.53 ± 0.019	0.692GlnAsn: 0.692 ± 0.025	1.597GlnPro: 1.597 ± 0.034	0.841GlnGln: 0.841 ± 0.032	2.225GlnArg: 2.225 ± 0.044	1.418GlnSer: 1.418 ± 0.036	1.707GlnThr: 1.707 ± 0.033	2.028GlnVal: 2.028 ± 0.044	0.284GlnTrp: 0.284 ± 0.018	0.463GlnTyr: 0.463 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.393ArgAla: 10.393 ± 0.11	0.502ArgCys: 0.502 ± 0.021	4.773ArgAsp: 4.773 ± 0.058	4.241ArgGlu: 4.241 ± 0.063	3.172ArgPhe: 3.172 ± 0.054	5.689ArgGly: 5.689 ± 0.073	1.864ArgHis: 1.864 ± 0.038	4.266ArgIle: 4.266 ± 0.058	1.903ArgLys: 1.903 ± 0.045	9.3ArgLeu: 9.3 ± 0.102	1.831ArgMet: 1.831 ± 0.04	1.735ArgAsn: 1.735 ± 0.035	4.728ArgPro: 4.728 ± 0.08	2.301ArgGln: 2.301 ± 0.045	7.095ArgArg: 7.095 ± 0.104	3.97ArgSer: 3.97 ± 0.058	4.369ArgThr: 4.369 ± 0.067	5.558ArgVal: 5.558 ± 0.059	1.014ArgTrp: 1.014 ± 0.031	1.717ArgTyr: 1.717 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.064SerAla: 6.064 ± 0.085	0.317SerCys: 0.317 ± 0.018	2.778SerAsp: 2.778 ± 0.046	2.523SerGlu: 2.523 ± 0.047	1.848SerPhe: 1.848 ± 0.039	5.274SerGly: 5.274 ± 0.069	0.917SerHis: 0.917 ± 0.032	2.091SerIle: 2.091 ± 0.044	1.22SerLys: 1.22 ± 0.036	5.41SerLeu: 5.41 ± 0.068	0.951SerMet: 0.951 ± 0.027	1.104SerAsn: 1.104 ± 0.028	2.903SerPro: 2.903 ± 0.047	1.341SerGln: 1.341 ± 0.031	3.559SerArg: 3.559 ± 0.05	2.255SerSer: 2.255 ± 0.048	2.423SerThr: 2.423 ± 0.048	3.732SerVal: 3.732 ± 0.061	0.559SerTrp: 0.559 ± 0.022	1.055SerTyr: 1.055 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	7.227ThrAla: 7.227 ± 0.101	0.451ThrCys: 0.451 ± 0.02	3.018ThrAsp: 3.018 ± 0.048	2.702ThrGlu: 2.702 ± 0.051	1.934ThrPhe: 1.934 ± 0.039	5.843ThrGly: 5.843 ± 0.098	1.025ThrHis: 1.025 ± 0.029	2.598ThrIle: 2.598 ± 0.049	1.413ThrLys: 1.413 ± 0.034	6.861ThrLeu: 6.861 ± 0.105	1.007ThrMet: 1.007 ± 0.029	1.233ThrAsn: 1.233 ± 0.04	3.693ThrPro: 3.693 ± 0.058	1.544ThrGln: 1.544 ± 0.033	3.926ThrArg: 3.926 ± 0.054	2.461ThrSer: 2.461 ± 0.052	3.096ThrThr: 3.096 ± 0.057	5.129ThrVal: 5.129 ± 0.074	0.69ThrTrp: 0.69 ± 0.025	1.241ThrTyr: 1.241 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	11.011ValAla: 11.011 ± 0.095	0.637ValCys: 0.637 ± 0.023	4.039ValAsp: 4.039 ± 0.062	4.561ValGlu: 4.561 ± 0.065	2.586ValPhe: 2.586 ± 0.041	5.946ValGly: 5.946 ± 0.066	1.393ValHis: 1.393 ± 0.033	3.395ValIle: 3.395 ± 0.057	1.921ValLys: 1.921 ± 0.039	7.692ValLeu: 7.692 ± 0.09	1.619ValMet: 1.619 ± 0.036	1.737ValAsn: 1.737 ± 0.041	4.424ValPro: 4.424 ± 0.064	1.988ValGln: 1.988 ± 0.041	5.734ValArg: 5.734 ± 0.082	4.241ValSer: 4.241 ± 0.059	4.964ValThr: 4.964 ± 0.084	6.463ValVal: 6.463 ± 0.085	0.894ValTrp: 0.894 ± 0.025	1.37ValTyr: 1.37 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.263TrpAla: 1.263 ± 0.036	0.143TrpCys: 0.143 ± 0.011	0.572TrpAsp: 0.572 ± 0.023	0.478TrpGlu: 0.478 ± 0.023	0.489TrpPhe: 0.489 ± 0.02	0.843TrpGly: 0.843 ± 0.028	0.305TrpHis: 0.305 ± 0.015	0.588TrpIle: 0.588 ± 0.022	0.325TrpLys: 0.325 ± 0.017	1.52TrpLeu: 1.52 ± 0.038	0.295TrpMet: 0.295 ± 0.016	0.349TrpAsn: 0.349 ± 0.016	0.629TrpPro: 0.629 ± 0.023	0.414TrpGln: 0.414 ± 0.017	1.266TrpArg: 1.266 ± 0.033	0.745TrpSer: 0.745 ± 0.024	0.845TrpThr: 0.845 ± 0.027	0.754TrpVal: 0.754 ± 0.024	0.202TrpTrp: 0.202 ± 0.012	0.229TrpTyr: 0.229 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.735TyrAla: 2.735 ± 0.05	0.191TyrCys: 0.191 ± 0.012	1.337TyrAsp: 1.337 ± 0.033	1.099TyrGlu: 1.099 ± 0.031	0.708TyrPhe: 0.708 ± 0.026	2.228TyrGly: 2.228 ± 0.052	0.405TyrHis: 0.405 ± 0.019	0.626TyrIle: 0.626 ± 0.021	0.434TyrLys: 0.434 ± 0.023	2.116TyrLeu: 2.116 ± 0.047	0.3TyrMet: 0.3 ± 0.016	0.467TyrAsn: 0.467 ± 0.02	0.977TyrPro: 0.977 ± 0.031	0.514TyrGln: 0.514 ± 0.019	1.841TyrArg: 1.841 ± 0.043	0.81TyrSer: 0.81 ± 0.028	1.017TyrThr: 1.017 ± 0.031	1.491TyrVal: 1.491 ± 0.037	0.267TyrTrp: 0.267 ± 0.015	0.459TyrTyr: 0.459 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4215 proteins (1312095 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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