Avibacterium endocarditidis
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1713 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S5AGJ4|A0A2S5AGJ4_9PAST 3-dehydroquinate synthase OS=Avibacterium endocarditidis OX=380674 GN=aroB PE=3 SV=1
MM1 pKa = 6.88 ITSPEE6 pKa = 4.18 DD7 pKa = 3.26 NAQPLQLAQTEE18 pKa = 4.66 LKK20 pKa = 10.88 NGTTEE25 pKa = 4.1 QVLLGWGFEE34 pKa = 4.36 SHH36 pKa = 7.22 TDD38 pKa = 3.77 TEE40 pKa = 4.87 WEE42 pKa = 4.86 GSCWWLLSNAAEE54 pKa = 4.28 EE55 pKa = 4.4 NTLTTSAVQNSPNFSTALSFSSISAQLPAEE85 pKa = 4.78 HH86 pKa = 6.6 YY87 pKa = 10.53 LQFGLDD93 pKa = 3.43 IGNLSDD99 pKa = 3.53
Molecular weight: 10.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.872
IPC_protein 3.706
Toseland 3.554
ProMoST 3.757
Dawson 3.681
Bjellqvist 3.923
Wikipedia 3.592
Rodwell 3.554
Grimsley 3.478
Solomon 3.643
Lehninger 3.592
Nozaki 3.821
DTASelect 3.897
Thurlkill 3.605
EMBOSS 3.605
Sillero 3.834
Patrickios 1.825
IPC_peptide 3.643
IPC2_peptide 3.808
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A2S5AKI3|A0A2S5AKI3_9PAST PTS trehalose transporter subunit IIBC OS=Avibacterium endocarditidis OX=380674 GN=C3Z13_01265 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 SRR14 pKa = 11.84 THH16 pKa = 6.4 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1713
0
1713
465782
21
2405
271.9
30.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.682 ± 0.063
1.063 ± 0.021
4.943 ± 0.045
6.232 ± 0.062
4.326 ± 0.047
6.733 ± 0.066
2.101 ± 0.031
6.743 ± 0.06
5.913 ± 0.047
10.593 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.031
4.713 ± 0.038
3.945 ± 0.037
5.369 ± 0.073
4.453 ± 0.045
5.641 ± 0.044
5.084 ± 0.04
6.611 ± 0.061
1.21 ± 0.025
3.204 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here