Mesorhizobium ephedrae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5676 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P7SS06|A0A2P7SS06_9RHIZ Maleylacetate reductase OS=Mesorhizobium ephedrae OX=2116701 GN=C7I84_02655 PE=4 SV=1
MM1 pKa = 8.34CMMCQTVSRR10 pKa = 11.84FASEE14 pKa = 4.83EE15 pKa = 3.39IDD17 pKa = 3.51APMPSGSVSVAPPQFDD33 pKa = 3.58QMRR36 pKa = 11.84PPYY39 pKa = 10.63DD40 pKa = 3.27MRR42 pKa = 11.84EE43 pKa = 4.01QEE45 pKa = 4.38PGDD48 pKa = 3.55AYY50 pKa = 10.77GAGRR54 pKa = 11.84TALISQVFDD63 pKa = 3.65AADD66 pKa = 3.21GRR68 pKa = 11.84YY69 pKa = 9.17EE70 pKa = 3.81FSGNQDD76 pKa = 1.95IDD78 pKa = 3.3ATLIGSRR85 pKa = 11.84WTLSNLTFSFPTSGTFYY102 pKa = 11.06ADD104 pKa = 2.81EE105 pKa = 5.3GYY107 pKa = 9.37PAGTEE112 pKa = 4.04PAEE115 pKa = 3.9HH116 pKa = 6.07VAFNAMQQDD125 pKa = 3.7AVRR128 pKa = 11.84YY129 pKa = 8.95ALSLVQSYY137 pKa = 9.71TNLTFTEE144 pKa = 4.53VAEE147 pKa = 4.47SATTHH152 pKa = 6.11AEE154 pKa = 3.8LRR156 pKa = 11.84FSQTSYY162 pKa = 11.71DD163 pKa = 4.2DD164 pKa = 3.8VPSAYY169 pKa = 10.38ANFPSSSEE177 pKa = 3.79QAGDD181 pKa = 3.08VWFGMTGQPFYY192 pKa = 10.0DD193 pKa = 4.08TPAMGNWGMATILHH207 pKa = 6.74EE208 pKa = 4.78IGHH211 pKa = 6.45ALGLKK216 pKa = 9.85HH217 pKa = 6.41GHH219 pKa = 5.78QDD221 pKa = 3.35YY222 pKa = 8.54TAVDD226 pKa = 3.99LAGEE230 pKa = 5.19GYY232 pKa = 10.68LDD234 pKa = 3.99PPGGGGPRR242 pKa = 11.84YY243 pKa = 10.14GSAEE247 pKa = 3.92LPANHH252 pKa = 7.57DD253 pKa = 3.76GQDD256 pKa = 3.41WSLMTYY262 pKa = 10.25RR263 pKa = 11.84SDD265 pKa = 3.25PGAPVAFQGEE275 pKa = 5.11GFNQPQTYY283 pKa = 7.44MQNDD287 pKa = 3.39IAALQYY293 pKa = 11.06LYY295 pKa = 11.07GANFDD300 pKa = 4.42TNSSATVYY308 pKa = 10.17SWNPTTGEE316 pKa = 3.81MSIDD320 pKa = 3.58GVGQGAPTSNKK331 pKa = 9.63ISMTLWDD338 pKa = 4.28GNGVDD343 pKa = 5.02TYY345 pKa = 11.67DD346 pKa = 3.56LSNYY350 pKa = 8.51ATDD353 pKa = 4.73LSIDD357 pKa = 3.98LAPGAFSTFSAAQLVNHH374 pKa = 7.11RR375 pKa = 11.84AYY377 pKa = 10.89SGGVAPAVGNVANALLYY394 pKa = 11.32NNDD397 pKa = 3.06TRR399 pKa = 11.84SLIEE403 pKa = 3.94NATGGSGNDD412 pKa = 3.6SIVGNQANNTLRR424 pKa = 11.84GNDD427 pKa = 3.68GGDD430 pKa = 3.37TLVGSFGSDD439 pKa = 2.64KK440 pKa = 11.07LYY442 pKa = 11.1GGSAADD448 pKa = 3.54TLYY451 pKa = 11.39GDD453 pKa = 5.7GIPGIPPGVEE463 pKa = 3.62LGDD466 pKa = 3.72GVVVKK471 pKa = 9.53PAGAGNISLATAIDD485 pKa = 3.7VTKK488 pKa = 10.49EE489 pKa = 3.39FSLASNANIIDD500 pKa = 3.98STATPHH506 pKa = 4.98VTISGTGDD514 pKa = 3.26GNRR517 pKa = 11.84DD518 pKa = 3.5YY519 pKa = 11.89YY520 pKa = 11.38KK521 pKa = 10.15LTVNAGTTLTFDD533 pKa = 3.54IDD535 pKa = 3.66NTSGVDD541 pKa = 3.49SFLFLYY547 pKa = 10.51DD548 pKa = 3.55PTSTAVAFDD557 pKa = 4.89DD558 pKa = 4.87DD559 pKa = 4.39SQVTEE564 pKa = 4.21GAGGSTSSFDD574 pKa = 3.85SYY576 pKa = 10.88LTYY579 pKa = 10.18QVKK582 pKa = 7.91TAGTYY587 pKa = 9.1YY588 pKa = 9.98IEE590 pKa = 4.12VGAYY594 pKa = 9.6VSSTSNGPIGSGALYY609 pKa = 10.23DD610 pKa = 4.02LQISAGGTPGSGGGGTGNDD629 pKa = 3.74TLDD632 pKa = 3.99GGTGDD637 pKa = 5.02DD638 pKa = 4.32SMAGGAGSDD647 pKa = 3.82VYY649 pKa = 10.94FVDD652 pKa = 3.59SAADD656 pKa = 3.6VVTEE660 pKa = 3.97TAGNGTADD668 pKa = 3.28RR669 pKa = 11.84VAARR673 pKa = 11.84ASYY676 pKa = 10.89ALAAGVSIEE685 pKa = 4.45LLTTTSSAGTTAIDD699 pKa = 4.26LSGNALKK706 pKa = 8.77QTTTGNAGANTLKK719 pKa = 10.95DD720 pKa = 3.47GGGVADD726 pKa = 4.77ALNGLGGDD734 pKa = 4.64DD735 pKa = 3.7IYY737 pKa = 11.58LVYY740 pKa = 10.65AAGTTVAEE748 pKa = 4.37AAGQGSDD755 pKa = 3.12RR756 pKa = 11.84VAAAVDD762 pKa = 4.22FALGAGVHH770 pKa = 6.02IEE772 pKa = 4.04VLTTTSSGGTAAIDD786 pKa = 3.53LTGNALQQDD795 pKa = 3.64ITGNAGDD802 pKa = 4.93NILHH806 pKa = 7.22DD807 pKa = 4.72GGAGAADD814 pKa = 3.82TLKK817 pKa = 11.17GLGGNDD823 pKa = 3.31TYY825 pKa = 11.04RR826 pKa = 11.84VYY828 pKa = 11.02NAADD832 pKa = 3.57VVVEE836 pKa = 4.33TAAQGVYY843 pKa = 10.11DD844 pKa = 3.98VVMAAVDD851 pKa = 3.8YY852 pKa = 10.97VLTAGAHH859 pKa = 4.62VEE861 pKa = 3.97RR862 pKa = 11.84LATNGTAGTSGIDD875 pKa = 3.3LTGNEE880 pKa = 4.09IGQEE884 pKa = 4.14VVGNSGANKK893 pKa = 10.1LDD895 pKa = 3.62GKK897 pKa = 11.17GGDD900 pKa = 3.74DD901 pKa = 3.59TMKK904 pKa = 11.04GLAGKK909 pKa = 8.04DD910 pKa = 3.35TLTGGTGKK918 pKa = 10.36DD919 pKa = 2.99LFVFSTALSASTNVDD934 pKa = 2.94TVTDD938 pKa = 3.94FNVPNDD944 pKa = 4.15TIGLDD949 pKa = 3.69DD950 pKa = 6.12AIFAALSLGTLVASAFWASTTGLAHH975 pKa = 7.11DD976 pKa = 4.06ASDD979 pKa = 4.26RR980 pKa = 11.84ILYY983 pKa = 7.57EE984 pKa = 3.95TDD986 pKa = 2.82TGALFYY992 pKa = 11.14DD993 pKa = 4.17ADD995 pKa = 3.97GTGAGARR1002 pKa = 11.84IQFAVLTGLPALTNADD1018 pKa = 3.56FAVII1022 pKa = 3.94

Molecular weight: 104.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P7S8P3|A0A2P7S8P3_9RHIZ NAD(P)-dependent oxidoreductase OS=Mesorhizobium ephedrae OX=2116701 GN=C7I84_14455 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84GVVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5676

0

5676

1760207

29

3292

310.1

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.982 ± 0.05

0.781 ± 0.01

5.777 ± 0.03

5.743 ± 0.033

3.87 ± 0.023

9.027 ± 0.043

1.962 ± 0.017

5.116 ± 0.024

3.237 ± 0.03

9.888 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.016

2.465 ± 0.02

5.164 ± 0.029

2.801 ± 0.015

7.272 ± 0.037

5.162 ± 0.025

5.107 ± 0.027

7.661 ± 0.025

1.323 ± 0.012

2.213 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski