Capybara microvirus Cap3_SP_319
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVS0|A0A4V1FVS0_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_319 OX=2585427 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.47 NQGSPILVRR11 pKa = 11.84 HH12 pKa = 5.76 YY13 pKa = 11.63 SDD15 pKa = 3.64 FHH17 pKa = 7.56 QNFISSPLSEE27 pKa = 4.43 FQNSLGSVDD36 pKa = 4.89 DD37 pKa = 4.36 YY38 pKa = 11.92 SFEE41 pKa = 4.29 AQLSSGLPLGSVNPTAFLGSPLSEE65 pKa = 3.71 EE66 pKa = 4.54 DD67 pKa = 3.4 ANKK70 pKa = 10.35 QITDD74 pKa = 3.14 IVEE77 pKa = 4.66 SINSS81 pKa = 3.49
Molecular weight: 8.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.313
IPC2_protein 4.304
IPC_protein 4.139
Toseland 3.973
ProMoST 4.291
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.05
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 3.541
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.158
Protein with the highest isoelectric point:
>tr|A0A4P8W8B3|A0A4P8W8B3_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_319 OX=2585427 PE=4 SV=1
MM1 pKa = 7.81 PCFTHH6 pKa = 6.14 KK7 pKa = 9.67 TLFNRR12 pKa = 11.84 VSRR15 pKa = 11.84 HH16 pKa = 5.27 LALTDD21 pKa = 3.62 KK22 pKa = 10.63 PFLEE26 pKa = 4.91 AGCGVCHH33 pKa = 7.41 DD34 pKa = 4.66 CLSKK38 pKa = 10.85 KK39 pKa = 10.29 RR40 pKa = 11.84 FSLYY44 pKa = 8.92 IRR46 pKa = 11.84 VWFEE50 pKa = 3.67 VEE52 pKa = 5.12 DD53 pKa = 3.46 ILKK56 pKa = 10.66 RR57 pKa = 11.84 GGFVLFPTYY66 pKa = 10.25 TYY68 pKa = 11.16 DD69 pKa = 5.19 DD70 pKa = 3.4 SHH72 pKa = 8.47 LPYY75 pKa = 10.03 FDD77 pKa = 4.64 SFSHH81 pKa = 5.0 QLSYY85 pKa = 11.42 GVVDD89 pKa = 3.75 SRR91 pKa = 11.84 DD92 pKa = 3.52 YY93 pKa = 11.35 KK94 pKa = 11.16 LNEE97 pKa = 4.08 VPCFDD102 pKa = 3.21 MSVFKK107 pKa = 10.81 GSKK110 pKa = 10.32 GLVKK114 pKa = 10.56 NLRR117 pKa = 11.84 NYY119 pKa = 10.69 AYY121 pKa = 10.28 RR122 pKa = 11.84 KK123 pKa = 8.62 YY124 pKa = 10.22 HH125 pKa = 5.99 VDD127 pKa = 4.04 GIKK130 pKa = 10.8 YY131 pKa = 8.44 LVCPEE136 pKa = 4.39 YY137 pKa = 11.02 GPSDD141 pKa = 3.68 GSTHH145 pKa = 6.43 RR146 pKa = 11.84 PHH148 pKa = 6.12 YY149 pKa = 10.3 HH150 pKa = 6.81 VMFFCPPCEE159 pKa = 4.02 GGEE162 pKa = 4.54 CVNSVYY168 pKa = 10.67 RR169 pKa = 11.84 QDD171 pKa = 3.66 LTRR174 pKa = 11.84 LALSLWQDD182 pKa = 2.84 KK183 pKa = 10.71 RR184 pKa = 11.84 KK185 pKa = 10.41 LGFADD190 pKa = 3.71 YY191 pKa = 10.74 SRR193 pKa = 11.84 EE194 pKa = 3.65 FGPYY198 pKa = 8.12 VTSLQAAKK206 pKa = 10.37 YY207 pKa = 8.61 ISKK210 pKa = 10.52 YY211 pKa = 6.51 CTKK214 pKa = 10.53 DD215 pKa = 2.79 ISEE218 pKa = 4.43 WLSVSPQLKK227 pKa = 9.5 SYY229 pKa = 10.91 LYY231 pKa = 8.62 GTSSVIDD238 pKa = 3.83 RR239 pKa = 11.84 NNLFLIRR246 pKa = 11.84 SYY248 pKa = 10.95 LPKK251 pKa = 10.21 PLWSDD256 pKa = 3.37 GLGISMVDD264 pKa = 3.39 YY265 pKa = 10.97 LKK267 pKa = 11.2 SLDD270 pKa = 3.52 GDD272 pKa = 4.15 SFVSAFTDD280 pKa = 4.62 GVHH283 pKa = 5.71 VPKK286 pKa = 10.65 DD287 pKa = 3.81 FKK289 pKa = 11.44 EE290 pKa = 4.37 DD291 pKa = 3.3 GSKK294 pKa = 10.78 NFYY297 pKa = 10.47 SVPRR301 pKa = 11.84 YY302 pKa = 9.56 IYY304 pKa = 11.1 NKK306 pKa = 9.67 VLYY309 pKa = 10.06 DD310 pKa = 3.76 KK311 pKa = 11.43 VGDD314 pKa = 3.77 SYY316 pKa = 11.79 LPNEE320 pKa = 4.45 FNHH323 pKa = 6.83 GYY325 pKa = 9.0 IKK327 pKa = 10.6 RR328 pKa = 11.84 IISRR332 pKa = 11.84 KK333 pKa = 9.33 LDD335 pKa = 3.48 LSFEE339 pKa = 4.33 RR340 pKa = 11.84 FNSDD344 pKa = 3.66 LLNASIVSDD353 pKa = 3.21 NGKK356 pKa = 10.19 NYY358 pKa = 10.51 NAFFNNLVSSYY369 pKa = 10.25 GKK371 pKa = 10.09 EE372 pKa = 3.55 YY373 pKa = 11.02 LFFSRR378 pKa = 11.84 YY379 pKa = 9.36 LYY381 pKa = 10.21 NVPLSWFSSHH391 pKa = 7.67 DD392 pKa = 3.08 IDD394 pKa = 5.69 VNVLDD399 pKa = 4.28 SSSIRR404 pKa = 11.84 DD405 pKa = 3.47 VVVSYY410 pKa = 11.01 LVDD413 pKa = 3.74 CSLKK417 pKa = 10.4 PRR419 pKa = 11.84 FIYY422 pKa = 9.63 PSKK425 pKa = 10.17 PWYY428 pKa = 10.2 KK429 pKa = 10.41 SDD431 pKa = 4.18 GFILGKK437 pKa = 10.15 NIHH440 pKa = 6.14 RR441 pKa = 11.84 LGYY444 pKa = 9.45 NVWVSDD450 pKa = 3.11 GKK452 pKa = 10.88 IFIRR456 pKa = 11.84 SPRR459 pKa = 11.84 NPQKK463 pKa = 11.01 LIFNRR468 pKa = 11.84 DD469 pKa = 3.18 SFNSLDD475 pKa = 3.72 YY476 pKa = 10.64 FRR478 pKa = 11.84 PPLIQHH484 pKa = 6.56 FFFIWKK490 pKa = 8.83 SYY492 pKa = 9.93 KK493 pKa = 10.18 VALQKK498 pKa = 11.17 ASLLKK503 pKa = 10.88 SKK505 pKa = 8.77 TLQDD509 pKa = 3.22 KK510 pKa = 10.75 GKK512 pKa = 10.56 AFYY515 pKa = 9.52 THH517 pKa = 5.97 VSTRR521 pKa = 11.84 KK522 pKa = 9.29 FRR524 pKa = 11.84 PLKK527 pKa = 10.43 FNSLTFTT534 pKa = 3.89
Molecular weight: 62.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.68
IPC2_protein 8.697
IPC_protein 8.565
Toseland 9.019
ProMoST 9.004
Dawson 9.414
Bjellqvist 9.268
Wikipedia 9.619
Rodwell 9.545
Grimsley 9.487
Solomon 9.428
Lehninger 9.385
Nozaki 9.282
DTASelect 9.18
Thurlkill 9.253
EMBOSS 9.516
Sillero 9.414
Patrickios 4.101
IPC_peptide 9.428
IPC2_peptide 8.009
IPC2.peptide.svr19 7.761
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1683
81
710
420.8
47.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.051 ± 2.056
1.307 ± 0.398
5.882 ± 0.63
4.04 ± 0.766
6.595 ± 1.259
6.298 ± 0.51
2.08 ± 0.556
5.169 ± 0.358
5.882 ± 0.941
9.269 ± 0.506
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.426 ± 0.59
7.605 ± 1.184
4.159 ± 0.651
3.09 ± 1.046
3.624 ± 0.77
11.23 ± 0.528
4.219 ± 1.02
6.358 ± 0.353
1.01 ± 0.214
5.704 ± 1.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here