Alteriqipengyuania halimionae
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2656 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4U1N4|A0A6I4U1N4_9SPHN Uncharacterized protein OS=Alteriqipengyuania halimionae OX=1926630 GN=GRI68_01335 PE=4 SV=1
MM1 pKa = 6.72 YY2 pKa = 10.55 SKK4 pKa = 11.12 YY5 pKa = 10.47 LVAAAGLLVVPPSGVAAQQAEE26 pKa = 4.32 QVDD29 pKa = 4.11 EE30 pKa = 4.97 RR31 pKa = 11.84 EE32 pKa = 4.11 TGAQSVADD40 pKa = 4.07 EE41 pKa = 4.66 TPPEE45 pKa = 4.39 SVPLADD51 pKa = 5.79 DD52 pKa = 4.55 GSDD55 pKa = 3.35 DD56 pKa = 3.82 HH57 pKa = 9.18 VSVSFGASVVSQYY70 pKa = 10.71 ISRR73 pKa = 11.84 GVAFAEE79 pKa = 4.13 PVSYY83 pKa = 10.46 QPYY86 pKa = 7.74 VTVSVALPEE95 pKa = 4.21 LTGGAITDD103 pKa = 3.34 ASVFVGNWNSMQSGSPGLGQPANGSLPGWYY133 pKa = 9.45 EE134 pKa = 3.41 ADD136 pKa = 4.04 LYY138 pKa = 11.49 VGGAIEE144 pKa = 3.96 IADD147 pKa = 4.16 RR148 pKa = 11.84 VAVSATYY155 pKa = 10.7 YY156 pKa = 10.41 RR157 pKa = 11.84 YY158 pKa = 9.78 FSPAGSFAEE167 pKa = 4.29 YY168 pKa = 10.85 NDD170 pKa = 3.47 FDD172 pKa = 4.16 LTISYY177 pKa = 10.48 DD178 pKa = 4.48 DD179 pKa = 3.61 SGLWGSAAQEE189 pKa = 4.21 RR190 pKa = 11.84 GFSIQPALRR199 pKa = 11.84 MVQEE203 pKa = 4.63 AGQPNRR209 pKa = 11.84 PDD211 pKa = 3.3 ALYY214 pKa = 9.17 VQPSIAPSVRR224 pKa = 11.84 FGPQDD229 pKa = 3.13 GGVTVSVPMAIGLSDD244 pKa = 3.48 SFYY247 pKa = 11.2 SGVNGDD253 pKa = 3.59 RR254 pKa = 11.84 PTFGFFRR261 pKa = 11.84 VGAGLAANPFGGPASNIRR279 pKa = 11.84 LNAGVDD285 pKa = 3.4 YY286 pKa = 11.09 WMLNDD291 pKa = 4.8 DD292 pKa = 4.39 VANGLDD298 pKa = 3.45 DD299 pKa = 4.3 TEE301 pKa = 4.28 WVARR305 pKa = 11.84 IGFSTGFF312 pKa = 3.39
Molecular weight: 32.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.163
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A6I4U4G4|A0A6I4U4G4_9SPHN Uncharacterized protein OS=Alteriqipengyuania halimionae OX=1926630 GN=GRI68_03965 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.99 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.0 KK41 pKa = 10.54 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2656
0
2656
856341
31
1671
322.4
35.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.554 ± 0.064
0.777 ± 0.014
6.643 ± 0.036
6.525 ± 0.046
3.606 ± 0.031
8.793 ± 0.049
1.845 ± 0.024
5.031 ± 0.027
3.239 ± 0.041
9.616 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.022
2.593 ± 0.028
5.058 ± 0.033
3.067 ± 0.024
7.098 ± 0.041
5.328 ± 0.037
5.21 ± 0.039
6.955 ± 0.036
1.392 ± 0.022
2.261 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here