Pseudomonas phage vB_PaeM_PAO1_Ab03

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Nankokuvirus; Pseudomonas virus Ab03

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1IU87|A0A0A1IU87_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_PAO1_Ab03 OX=1548901 GN=ORF153 PE=4 SV=1
MM1 pKa = 7.5ISLVLTLCTALACDD15 pKa = 5.17DD16 pKa = 3.96YY17 pKa = 11.93VIAQDD22 pKa = 3.94SDD24 pKa = 3.75ADD26 pKa = 4.2IINHH30 pKa = 5.03TLYY33 pKa = 11.04VEE35 pKa = 4.31SEE37 pKa = 4.26EE38 pKa = 6.27LGDD41 pKa = 4.1AWVAPNANDD50 pKa = 4.45RR51 pKa = 11.84IGRR54 pKa = 11.84YY55 pKa = 8.0LARR58 pKa = 11.84FNISTPPAMVVDD70 pKa = 3.46YY71 pKa = 10.75DD72 pKa = 3.97YY73 pKa = 11.35TVEE76 pKa = 4.2GNN78 pKa = 3.28

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1IVH0|A0A0A1IVH0_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeM_PAO1_Ab03 OX=1548901 GN=ORF150 PE=4 SV=1
MM1 pKa = 7.38QIHH4 pKa = 6.41FSSRR8 pKa = 11.84PAARR12 pKa = 11.84AFVKK16 pKa = 10.61GSAVPRR22 pKa = 11.84QVADD26 pKa = 3.55NGAEE30 pKa = 3.89ASRR33 pKa = 11.84RR34 pKa = 11.84WGVKK38 pKa = 10.03LSISPCVRR46 pKa = 11.84VLGCDD51 pKa = 3.09HH52 pKa = 7.16FATGKK57 pKa = 7.43TGNMVPVYY65 pKa = 10.29IKK67 pKa = 10.9

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

155

0

155

25546

37

885

164.8

18.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.714 ± 0.294

1.394 ± 0.113

5.989 ± 0.178

7.555 ± 0.231

3.625 ± 0.123

6.995 ± 0.192

2.047 ± 0.107

4.725 ± 0.125

5.77 ± 0.238

8.722 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.103

3.981 ± 0.18

4.361 ± 0.179

4.106 ± 0.134

5.684 ± 0.143

5.539 ± 0.163

5.504 ± 0.218

7.093 ± 0.194

1.828 ± 0.102

3.386 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski