Amino acid dipepetide frequency for Pseudomonas phage vB_PaeM_PAO1_Ab03

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.299AlaAla: 8.299 ± 0.676
1.057AlaCys: 1.057 ± 0.22
5.363AlaAsp: 5.363 ± 0.456
5.676AlaGlu: 5.676 ± 0.471
2.31AlaPhe: 2.31 ± 0.282
6.498AlaGly: 6.498 ± 0.61
1.762AlaHis: 1.762 ± 0.249
4.032AlaIle: 4.032 ± 0.326
4.893AlaLys: 4.893 ± 0.551
7.908AlaLeu: 7.908 ± 0.636
3.445AlaMet: 3.445 ± 0.327
3.367AlaAsn: 3.367 ± 0.413
3.014AlaPro: 3.014 ± 0.384
4.15AlaGln: 4.15 ± 0.404
4.854AlaArg: 4.854 ± 0.436
5.011AlaSer: 5.011 ± 0.503
5.05AlaThr: 5.05 ± 0.579
6.146AlaVal: 6.146 ± 0.587
2.153AlaTrp: 2.153 ± 0.261
2.153AlaTyr: 2.153 ± 0.27
0.0AlaXaa: 0.0 ± 0.0
Cys
0.9CysAla: 0.9 ± 0.191
0.313CysCys: 0.313 ± 0.106
0.822CysAsp: 0.822 ± 0.192
1.174CysGlu: 1.174 ± 0.221
0.587CysPhe: 0.587 ± 0.158
1.174CysGly: 1.174 ± 0.222
0.431CysHis: 0.431 ± 0.127
0.587CysIle: 0.587 ± 0.143
0.626CysLys: 0.626 ± 0.163
0.861CysLeu: 0.861 ± 0.182
0.431CysMet: 0.431 ± 0.128
0.548CysAsn: 0.548 ± 0.133
0.861CysPro: 0.861 ± 0.17
0.783CysGln: 0.783 ± 0.197
1.214CysArg: 1.214 ± 0.205
0.822CysSer: 0.822 ± 0.199
0.509CysThr: 0.509 ± 0.133
0.391CysVal: 0.391 ± 0.123
0.509CysTrp: 0.509 ± 0.161
0.391CysTyr: 0.391 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.737AspAla: 4.737 ± 0.401
1.057AspCys: 1.057 ± 0.24
3.327AspAsp: 3.327 ± 0.34
4.737AspGlu: 4.737 ± 0.416
2.74AspPhe: 2.74 ± 0.372
4.502AspGly: 4.502 ± 0.458
1.292AspHis: 1.292 ± 0.21
4.15AspIle: 4.15 ± 0.369
2.975AspLys: 2.975 ± 0.345
5.206AspLeu: 5.206 ± 0.433
1.683AspMet: 1.683 ± 0.258
2.271AspAsn: 2.271 ± 0.326
2.075AspPro: 2.075 ± 0.259
1.957AspGln: 1.957 ± 0.242
3.719AspArg: 3.719 ± 0.403
2.662AspSer: 2.662 ± 0.342
3.367AspThr: 3.367 ± 0.418
4.306AspVal: 4.306 ± 0.345
1.292AspTrp: 1.292 ± 0.158
1.84AspTyr: 1.84 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
7.829GluAla: 7.829 ± 0.494
1.057GluCys: 1.057 ± 0.195
4.267GluAsp: 4.267 ± 0.439
6.342GluGlu: 6.342 ± 0.603
3.171GluPhe: 3.171 ± 0.36
5.481GluGly: 5.481 ± 0.518
1.292GluHis: 1.292 ± 0.223
3.484GluIle: 3.484 ± 0.335
4.619GluLys: 4.619 ± 0.501
6.42GluLeu: 6.42 ± 0.538
2.74GluMet: 2.74 ± 0.291
2.623GluAsn: 2.623 ± 0.353
2.349GluPro: 2.349 ± 0.33
3.406GluGln: 3.406 ± 0.378
3.836GluArg: 3.836 ± 0.349
3.523GluSer: 3.523 ± 0.364
2.74GluThr: 2.74 ± 0.303
6.42GluVal: 6.42 ± 0.47
1.488GluTrp: 1.488 ± 0.227
2.466GluTyr: 2.466 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
2.349PheAla: 2.349 ± 0.303
0.548PheCys: 0.548 ± 0.157
2.623PheAsp: 2.623 ± 0.346
3.484PheGlu: 3.484 ± 0.385
1.253PhePhe: 1.253 ± 0.199
2.466PheGly: 2.466 ± 0.302
0.783PheHis: 0.783 ± 0.193
1.605PheIle: 1.605 ± 0.272
1.996PheLys: 1.996 ± 0.315
3.367PheLeu: 3.367 ± 0.417
0.822PheMet: 0.822 ± 0.174
1.409PheAsn: 1.409 ± 0.201
2.271PhePro: 2.271 ± 0.302
1.722PheGln: 1.722 ± 0.264
1.762PheArg: 1.762 ± 0.221
2.623PheSer: 2.623 ± 0.275
1.996PheThr: 1.996 ± 0.309
1.918PheVal: 1.918 ± 0.281
0.391PheTrp: 0.391 ± 0.148
0.861PheTyr: 0.861 ± 0.144
0.0PheXaa: 0.0 ± 0.0
Gly
5.363GlyAla: 5.363 ± 0.546
0.861GlyCys: 0.861 ± 0.156
4.11GlyAsp: 4.11 ± 0.344
4.306GlyGlu: 4.306 ± 0.458
3.014GlyPhe: 3.014 ± 0.317
4.932GlyGly: 4.932 ± 0.434
1.37GlyHis: 1.37 ± 0.226
3.758GlyIle: 3.758 ± 0.413
4.11GlyLys: 4.11 ± 0.423
5.676GlyLeu: 5.676 ± 0.557
2.505GlyMet: 2.505 ± 0.35
3.171GlyAsn: 3.171 ± 0.381
2.192GlyPro: 2.192 ± 0.297
2.545GlyGln: 2.545 ± 0.376
3.562GlyArg: 3.562 ± 0.38
4.071GlySer: 4.071 ± 0.423
3.641GlyThr: 3.641 ± 0.529
5.598GlyVal: 5.598 ± 0.517
1.605GlyTrp: 1.605 ± 0.279
3.562GlyTyr: 3.562 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.762HisAla: 1.762 ± 0.243
0.274HisCys: 0.274 ± 0.091
1.488HisAsp: 1.488 ± 0.25
1.527HisGlu: 1.527 ± 0.21
0.587HisPhe: 0.587 ± 0.16
1.722HisGly: 1.722 ± 0.244
0.313HisHis: 0.313 ± 0.104
0.979HisIle: 0.979 ± 0.18
0.783HisLys: 0.783 ± 0.183
2.075HisLeu: 2.075 ± 0.311
0.626HisMet: 0.626 ± 0.144
0.665HisAsn: 0.665 ± 0.149
1.448HisPro: 1.448 ± 0.232
0.744HisGln: 0.744 ± 0.187
1.292HisArg: 1.292 ± 0.211
1.018HisSer: 1.018 ± 0.182
1.018HisThr: 1.018 ± 0.2
1.292HisVal: 1.292 ± 0.208
0.509HisTrp: 0.509 ± 0.124
0.352HisTyr: 0.352 ± 0.108
0.0HisXaa: 0.0 ± 0.0
Ile
3.601IleAla: 3.601 ± 0.326
0.783IleCys: 0.783 ± 0.164
2.74IleAsp: 2.74 ± 0.302
3.876IleGlu: 3.876 ± 0.431
1.331IlePhe: 1.331 ± 0.267
3.014IleGly: 3.014 ± 0.393
0.979IleHis: 0.979 ± 0.203
2.192IleIle: 2.192 ± 0.276
2.349IleLys: 2.349 ± 0.242
4.345IleLeu: 4.345 ± 0.404
0.94IleMet: 0.94 ± 0.207
2.231IleAsn: 2.231 ± 0.388
3.132IlePro: 3.132 ± 0.337
2.075IleGln: 2.075 ± 0.252
2.74IleArg: 2.74 ± 0.379
2.466IleSer: 2.466 ± 0.279
3.445IleThr: 3.445 ± 0.383
2.858IleVal: 2.858 ± 0.392
0.665IleTrp: 0.665 ± 0.147
1.488IleTyr: 1.488 ± 0.265
0.0IleXaa: 0.0 ± 0.0
Lys
6.42LysAla: 6.42 ± 0.576
0.509LysCys: 0.509 ± 0.135
3.367LysAsp: 3.367 ± 0.388
4.619LysGlu: 4.619 ± 0.423
1.722LysPhe: 1.722 ± 0.246
4.228LysGly: 4.228 ± 0.337
1.37LysHis: 1.37 ± 0.265
2.388LysIle: 2.388 ± 0.301
3.719LysLys: 3.719 ± 0.432
4.972LysLeu: 4.972 ± 0.465
2.349LysMet: 2.349 ± 0.352
2.584LysAsn: 2.584 ± 0.295
1.722LysPro: 1.722 ± 0.236
2.036LysGln: 2.036 ± 0.272
3.21LysArg: 3.21 ± 0.388
2.584LysSer: 2.584 ± 0.311
2.505LysThr: 2.505 ± 0.337
4.658LysVal: 4.658 ± 0.487
0.822LysTrp: 0.822 ± 0.173
1.918LysTyr: 1.918 ± 0.319
0.0LysXaa: 0.0 ± 0.0
Leu
8.691LeuAla: 8.691 ± 0.598
1.214LeuCys: 1.214 ± 0.224
6.068LeuAsp: 6.068 ± 0.483
6.812LeuGlu: 6.812 ± 0.587
2.936LeuPhe: 2.936 ± 0.333
5.755LeuGly: 5.755 ± 0.462
1.488LeuHis: 1.488 ± 0.212
3.719LeuIle: 3.719 ± 0.39
5.363LeuLys: 5.363 ± 0.473
6.381LeuLeu: 6.381 ± 0.626
2.192LeuMet: 2.192 ± 0.251
3.445LeuAsn: 3.445 ± 0.357
4.345LeuPro: 4.345 ± 0.489
3.445LeuGln: 3.445 ± 0.321
5.872LeuArg: 5.872 ± 0.535
5.441LeuSer: 5.441 ± 0.486
4.932LeuThr: 4.932 ± 0.372
4.893LeuVal: 4.893 ± 0.478
1.057LeuTrp: 1.057 ± 0.178
3.171LeuTyr: 3.171 ± 0.404
0.0LeuXaa: 0.0 ± 0.0
Met
3.093MetAla: 3.093 ± 0.409
0.431MetCys: 0.431 ± 0.127
1.722MetAsp: 1.722 ± 0.225
2.466MetGlu: 2.466 ± 0.309
0.783MetPhe: 0.783 ± 0.171
1.801MetGly: 1.801 ± 0.273
0.509MetHis: 0.509 ± 0.127
1.527MetIle: 1.527 ± 0.279
2.192MetLys: 2.192 ± 0.304
2.192MetLeu: 2.192 ± 0.307
0.705MetMet: 0.705 ± 0.175
1.135MetAsn: 1.135 ± 0.206
1.448MetPro: 1.448 ± 0.242
1.214MetGln: 1.214 ± 0.21
1.527MetArg: 1.527 ± 0.219
2.114MetSer: 2.114 ± 0.298
1.918MetThr: 1.918 ± 0.244
1.801MetVal: 1.801 ± 0.261
0.391MetTrp: 0.391 ± 0.114
0.861MetTyr: 0.861 ± 0.179
0.0MetXaa: 0.0 ± 0.0
Asn
3.014AsnAla: 3.014 ± 0.32
0.352AsnCys: 0.352 ± 0.124
1.762AsnAsp: 1.762 ± 0.214
2.584AsnGlu: 2.584 ± 0.31
1.409AsnPhe: 1.409 ± 0.226
3.132AsnGly: 3.132 ± 0.38
1.096AsnHis: 1.096 ± 0.193
2.427AsnIle: 2.427 ± 0.29
2.31AsnLys: 2.31 ± 0.304
3.523AsnLeu: 3.523 ± 0.443
0.822AsnMet: 0.822 ± 0.176
1.605AsnAsn: 1.605 ± 0.289
2.545AsnPro: 2.545 ± 0.302
1.644AsnGln: 1.644 ± 0.293
1.996AsnArg: 1.996 ± 0.256
2.779AsnSer: 2.779 ± 0.384
2.075AsnThr: 2.075 ± 0.322
2.858AsnVal: 2.858 ± 0.273
0.626AsnTrp: 0.626 ± 0.182
1.253AsnTyr: 1.253 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
3.758ProAla: 3.758 ± 0.433
0.665ProCys: 0.665 ± 0.21
2.349ProAsp: 2.349 ± 0.301
4.306ProGlu: 4.306 ± 0.352
1.762ProPhe: 1.762 ± 0.243
3.093ProGly: 3.093 ± 0.389
0.94ProHis: 0.94 ± 0.17
1.448ProIle: 1.448 ± 0.221
2.662ProLys: 2.662 ± 0.338
3.641ProLeu: 3.641 ± 0.454
0.9ProMet: 0.9 ± 0.166
1.801ProAsn: 1.801 ± 0.224
1.84ProPro: 1.84 ± 0.323
1.566ProGln: 1.566 ± 0.266
1.879ProArg: 1.879 ± 0.329
2.623ProSer: 2.623 ± 0.347
2.662ProThr: 2.662 ± 0.3
3.406ProVal: 3.406 ± 0.346
0.783ProTrp: 0.783 ± 0.182
1.527ProTyr: 1.527 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
4.893GlnAla: 4.893 ± 0.425
0.352GlnCys: 0.352 ± 0.142
2.036GlnAsp: 2.036 ± 0.262
3.367GlnGlu: 3.367 ± 0.364
1.448GlnPhe: 1.448 ± 0.237
2.74GlnGly: 2.74 ± 0.307
1.174GlnHis: 1.174 ± 0.257
1.84GlnIle: 1.84 ± 0.295
1.762GlnLys: 1.762 ± 0.282
3.053GlnLeu: 3.053 ± 0.319
1.683GlnMet: 1.683 ± 0.305
1.448GlnAsn: 1.448 ± 0.243
1.253GlnPro: 1.253 ± 0.244
2.114GlnGln: 2.114 ± 0.269
2.271GlnArg: 2.271 ± 0.28
1.722GlnSer: 1.722 ± 0.238
2.114GlnThr: 2.114 ± 0.282
3.523GlnVal: 3.523 ± 0.378
0.9GlnTrp: 0.9 ± 0.187
1.37GlnTyr: 1.37 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
4.815ArgAla: 4.815 ± 0.448
0.979ArgCys: 0.979 ± 0.206
3.445ArgAsp: 3.445 ± 0.389
4.541ArgGlu: 4.541 ± 0.464
2.466ArgPhe: 2.466 ± 0.306
3.014ArgGly: 3.014 ± 0.27
1.214ArgHis: 1.214 ± 0.205
2.858ArgIle: 2.858 ± 0.278
3.601ArgLys: 3.601 ± 0.434
5.794ArgLeu: 5.794 ± 0.514
1.683ArgMet: 1.683 ± 0.227
2.192ArgAsn: 2.192 ± 0.238
2.075ArgPro: 2.075 ± 0.266
2.858ArgGln: 2.858 ± 0.265
3.327ArgArg: 3.327 ± 0.424
2.075ArgSer: 2.075 ± 0.226
3.406ArgThr: 3.406 ± 0.384
3.836ArgVal: 3.836 ± 0.408
0.822ArgTrp: 0.822 ± 0.175
1.84ArgTyr: 1.84 ± 0.243
0.0ArgXaa: 0.0 ± 0.0
Ser
3.719SerAla: 3.719 ± 0.464
1.018SerCys: 1.018 ± 0.188
2.975SerAsp: 2.975 ± 0.272
3.484SerGlu: 3.484 ± 0.33
2.192SerPhe: 2.192 ± 0.326
4.306SerGly: 4.306 ± 0.367
0.626SerHis: 0.626 ± 0.129
2.075SerIle: 2.075 ± 0.27
3.719SerLys: 3.719 ± 0.361
4.815SerLeu: 4.815 ± 0.438
1.605SerMet: 1.605 ± 0.275
2.153SerAsn: 2.153 ± 0.285
3.093SerPro: 3.093 ± 0.42
2.153SerGln: 2.153 ± 0.243
3.327SerArg: 3.327 ± 0.309
3.21SerSer: 3.21 ± 0.409
3.249SerThr: 3.249 ± 0.357
4.071SerVal: 4.071 ± 0.494
1.214SerTrp: 1.214 ± 0.201
2.388SerTyr: 2.388 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
4.58ThrAla: 4.58 ± 0.461
0.705ThrCys: 0.705 ± 0.185
3.406ThrAsp: 3.406 ± 0.418
3.053ThrGlu: 3.053 ± 0.336
1.918ThrPhe: 1.918 ± 0.294
4.071ThrGly: 4.071 ± 0.486
1.096ThrHis: 1.096 ± 0.207
2.819ThrIle: 2.819 ± 0.365
2.192ThrLys: 2.192 ± 0.3
6.107ThrLeu: 6.107 ± 0.464
1.605ThrMet: 1.605 ± 0.265
2.075ThrAsn: 2.075 ± 0.362
3.014ThrPro: 3.014 ± 0.316
2.114ThrGln: 2.114 ± 0.309
2.819ThrArg: 2.819 ± 0.305
3.562ThrSer: 3.562 ± 0.37
2.936ThrThr: 2.936 ± 0.376
4.345ThrVal: 4.345 ± 0.383
0.861ThrTrp: 0.861 ± 0.178
1.762ThrTyr: 1.762 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
5.559ValAla: 5.559 ± 0.578
0.744ValCys: 0.744 ± 0.15
3.797ValAsp: 3.797 ± 0.384
6.068ValGlu: 6.068 ± 0.516
3.093ValPhe: 3.093 ± 0.389
4.502ValGly: 4.502 ± 0.429
1.488ValHis: 1.488 ± 0.28
3.445ValIle: 3.445 ± 0.316
4.424ValLys: 4.424 ± 0.49
6.146ValLeu: 6.146 ± 0.438
2.153ValMet: 2.153 ± 0.24
2.74ValAsn: 2.74 ± 0.312
3.014ValPro: 3.014 ± 0.299
2.505ValGln: 2.505 ± 0.284
3.915ValArg: 3.915 ± 0.407
4.228ValSer: 4.228 ± 0.432
3.836ValThr: 3.836 ± 0.38
5.402ValVal: 5.402 ± 0.547
1.214ValTrp: 1.214 ± 0.28
2.662ValTyr: 2.662 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
1.409TrpAla: 1.409 ± 0.224
0.235TrpCys: 0.235 ± 0.086
1.331TrpAsp: 1.331 ± 0.198
1.253TrpGlu: 1.253 ± 0.213
0.509TrpPhe: 0.509 ± 0.14
1.253TrpGly: 1.253 ± 0.264
0.431TrpHis: 0.431 ± 0.112
0.744TrpIle: 0.744 ± 0.176
1.488TrpLys: 1.488 ± 0.267
1.996TrpLeu: 1.996 ± 0.268
0.47TrpMet: 0.47 ± 0.174
0.783TrpAsn: 0.783 ± 0.16
0.352TrpPro: 0.352 ± 0.114
0.47TrpGln: 0.47 ± 0.133
1.135TrpArg: 1.135 ± 0.249
1.096TrpSer: 1.096 ± 0.185
1.174TrpThr: 1.174 ± 0.222
0.861TrpVal: 0.861 ± 0.173
0.47TrpTrp: 0.47 ± 0.157
0.822TrpTyr: 0.822 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.349TyrAla: 2.349 ± 0.306
0.783TyrCys: 0.783 ± 0.19
2.897TyrAsp: 2.897 ± 0.325
1.879TyrGlu: 1.879 ± 0.267
1.018TyrPhe: 1.018 ± 0.181
2.271TyrGly: 2.271 ± 0.291
0.822TyrHis: 0.822 ± 0.164
1.174TyrIle: 1.174 ± 0.228
1.918TyrLys: 1.918 ± 0.253
2.74TyrLeu: 2.74 ± 0.33
0.431TyrMet: 0.431 ± 0.129
1.566TyrAsn: 1.566 ± 0.224
1.566TyrPro: 1.566 ± 0.224
1.566TyrGln: 1.566 ± 0.219
2.584TyrArg: 2.584 ± 0.322
1.762TyrSer: 1.762 ± 0.263
2.466TyrThr: 2.466 ± 0.317
2.349TyrVal: 2.349 ± 0.289
0.509TyrTrp: 0.509 ± 0.13
1.174TyrTyr: 1.174 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 155 proteins (25546 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski