Amylolactobacillus amylophilus DSM 20533 = JCM 1125

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Amylolactobacillus; Amylolactobacillus amylophilus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1YJW0|A0A0R1YJW0_9LACO Bac_transf domain-containing protein OS=Amylolactobacillus amylophilus DSM 20533 = JCM 1125 OX=1423721 GN=FD40_GL000441 PE=3 SV=1
MM1 pKa = 6.1TTAKK5 pKa = 9.67IVYY8 pKa = 10.54ASMTGNNEE16 pKa = 3.8EE17 pKa = 4.18IANILDD23 pKa = 3.6EE24 pKa = 4.55EE25 pKa = 4.4LTDD28 pKa = 4.14RR29 pKa = 11.84GVTTSLSDD37 pKa = 3.35ISFTPASEE45 pKa = 3.99YY46 pKa = 11.19LNYY49 pKa = 10.68DD50 pKa = 3.36LTLMVVYY57 pKa = 9.06TYY59 pKa = 11.57GEE61 pKa = 4.21GEE63 pKa = 4.41MPDD66 pKa = 3.81EE67 pKa = 4.44VADD70 pKa = 4.76FYY72 pKa = 11.91DD73 pKa = 5.4DD74 pKa = 4.86LAEE77 pKa = 4.88LDD79 pKa = 3.93LTGKK83 pKa = 10.28VYY85 pKa = 11.08AVMGSGDD92 pKa = 3.8LFYY95 pKa = 11.06EE96 pKa = 3.83EE97 pKa = 5.72HH98 pKa = 6.15YY99 pKa = 10.9CEE101 pKa = 4.01TVDD104 pKa = 4.44YY105 pKa = 10.89FEE107 pKa = 5.34QLFTKK112 pKa = 10.5IGATKK117 pKa = 10.09GAEE120 pKa = 3.75PLKK123 pKa = 10.85INLEE127 pKa = 3.95ADD129 pKa = 3.53EE130 pKa = 6.51ADD132 pKa = 3.73MDD134 pKa = 4.73NIAAVADD141 pKa = 3.88SVLAKK146 pKa = 10.67LNEE149 pKa = 4.17KK150 pKa = 10.28AA151 pKa = 4.25

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1YNW8|A0A0R1YNW8_9LACO Cell wall-associated glycoside hydrolase (NLP P60 protein) OS=Amylolactobacillus amylophilus DSM 20533 = JCM 1125 OX=1423721 GN=FD40_GL001316 PE=3 SV=1
MM1 pKa = 6.05TTKK4 pKa = 9.88RR5 pKa = 11.84TYY7 pKa = 9.64QPKK10 pKa = 8.58KK11 pKa = 7.47RR12 pKa = 11.84HH13 pKa = 5.71RR14 pKa = 11.84EE15 pKa = 3.79RR16 pKa = 11.84VHH18 pKa = 7.7GFMKK22 pKa = 10.56RR23 pKa = 11.84MSTKK27 pKa = 9.52NGRR30 pKa = 11.84RR31 pKa = 11.84VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.05GRR41 pKa = 11.84KK42 pKa = 8.7VLSAA46 pKa = 4.05

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1532

0

1532

452247

46

1524

295.2

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.807 ± 0.054

0.325 ± 0.012

5.705 ± 0.059

6.167 ± 0.068

4.494 ± 0.049

6.485 ± 0.059

1.949 ± 0.028

7.367 ± 0.066

6.492 ± 0.059

9.921 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.03

5.014 ± 0.046

3.486 ± 0.034

4.493 ± 0.047

4.172 ± 0.046

5.802 ± 0.049

6.149 ± 0.046

7.271 ± 0.049

0.869 ± 0.02

3.513 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski