Tindallia californiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Tindallia

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3I875|A0A1H3I875_9CLOT Pyrimidine-specific ribonucleoside hydrolase OS=Tindallia californiensis OX=159292 GN=SAMN05192546_10145 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84KK3 pKa = 9.5RR4 pKa = 11.84KK5 pKa = 9.63NLFCMLLVVLLVFSLLAGCGSPAEE29 pKa = 4.04EE30 pKa = 4.28DD31 pKa = 3.31AVEE34 pKa = 4.18EE35 pKa = 4.21VSEE38 pKa = 4.18EE39 pKa = 4.47TITEE43 pKa = 4.0PSEE46 pKa = 4.15EE47 pKa = 3.95EE48 pKa = 4.15VVITVAAGAVGQEE61 pKa = 4.29LEE63 pKa = 4.35LTQLAAEE70 pKa = 5.06MYY72 pKa = 10.0MDD74 pKa = 5.79DD75 pKa = 4.18NPNVIVNVLDD85 pKa = 4.14TPDD88 pKa = 3.64LAQDD92 pKa = 3.47RR93 pKa = 11.84LGLYY97 pKa = 10.05LQFFEE102 pKa = 4.52AQSAEE107 pKa = 3.73VDD109 pKa = 3.17IYY111 pKa = 10.94QIDD114 pKa = 4.47VIWPGDD120 pKa = 3.8LEE122 pKa = 4.25EE123 pKa = 5.31HH124 pKa = 6.49FVDD127 pKa = 5.06LYY129 pKa = 11.18EE130 pKa = 4.42YY131 pKa = 10.88GADD134 pKa = 3.38AVVEE138 pKa = 4.15DD139 pKa = 4.75HH140 pKa = 7.13FPAIVEE146 pKa = 4.2NNTVNGRR153 pKa = 11.84LVAIPWFTDD162 pKa = 2.64AGLLYY167 pKa = 10.83YY168 pKa = 9.89RR169 pKa = 11.84TDD171 pKa = 3.53LLEE174 pKa = 5.54KK175 pKa = 10.72YY176 pKa = 10.36DD177 pKa = 4.05FAPPEE182 pKa = 3.92TWEE185 pKa = 4.12EE186 pKa = 3.97LVEE189 pKa = 4.0QAQTIQQGEE198 pKa = 4.16RR199 pKa = 11.84DD200 pKa = 3.9EE201 pKa = 5.15GNQDD205 pKa = 2.94FVGYY209 pKa = 9.37VWQGDD214 pKa = 3.81AYY216 pKa = 10.46EE217 pKa = 4.82GLTCNALEE225 pKa = 4.8WIASNNGGTIISPEE239 pKa = 3.88GVITVNNEE247 pKa = 3.13NAAEE251 pKa = 4.47IIDD254 pKa = 4.16MAATWVGTISPEE266 pKa = 4.11GVTGMAEE273 pKa = 3.67EE274 pKa = 4.45DD275 pKa = 4.81ARR277 pKa = 11.84AIWQGGNAAFMRR289 pKa = 11.84NWPYY293 pKa = 11.12AYY295 pKa = 10.66SLGQEE300 pKa = 4.35DD301 pKa = 4.54DD302 pKa = 4.07SAVKK306 pKa = 10.52DD307 pKa = 3.64IFDD310 pKa = 4.22VSPLPAGTNGEE321 pKa = 4.12PAATLGGWQLAVSKK335 pKa = 10.86YY336 pKa = 10.79SEE338 pKa = 4.42NPDD341 pKa = 3.11VAADD345 pKa = 3.56VALFMASYY353 pKa = 11.38DD354 pKa = 3.85MQKK357 pKa = 9.32MRR359 pKa = 11.84AVDD362 pKa = 3.9GSLNPTIMDD371 pKa = 4.47LYY373 pKa = 10.39EE374 pKa = 6.19DD375 pKa = 4.26EE376 pKa = 5.71DD377 pKa = 4.03VLEE380 pKa = 4.45AAPFFGSLYY389 pKa = 10.61DD390 pKa = 3.52VFINAVARR398 pKa = 11.84PSTASAPNYY407 pKa = 9.41NAVSTAFFRR416 pKa = 11.84AVHH419 pKa = 5.82NVLNGNTDD427 pKa = 2.96AATALEE433 pKa = 4.19EE434 pKa = 5.27LEE436 pKa = 5.98LDD438 pKa = 3.88LQDD441 pKa = 3.04ITGFDD446 pKa = 3.41IEE448 pKa = 4.35

Molecular weight: 49.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3MEV2|A0A1H3MEV2_9CLOT Predicted Fe-Mo cluster-binding protein NifX family OS=Tindallia californiensis OX=159292 GN=SAMN05192546_104110 PE=4 SV=1
MM1 pKa = 7.17YY2 pKa = 10.23NYY4 pKa = 10.26RR5 pKa = 11.84CRR7 pKa = 11.84DD8 pKa = 2.83RR9 pKa = 11.84WNGKK13 pKa = 9.0RR14 pKa = 11.84YY15 pKa = 7.78YY16 pKa = 10.38QRR18 pKa = 11.84TVKK21 pKa = 10.6KK22 pKa = 9.96IGKK25 pKa = 9.64DD26 pKa = 3.02SDD28 pKa = 3.48RR29 pKa = 11.84QGVYY33 pKa = 9.81NAGMDD38 pKa = 3.68RR39 pKa = 11.84RR40 pKa = 11.84ILSMMVIRR48 pKa = 5.67

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2832

0

2832

931524

29

3104

328.9

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.904 ± 0.046

0.928 ± 0.018

5.11 ± 0.036

8.206 ± 0.058

4.01 ± 0.035

6.906 ± 0.042

2.167 ± 0.022

7.947 ± 0.042

6.827 ± 0.053

9.564 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.306 ± 0.027

4.163 ± 0.033

3.596 ± 0.025

3.823 ± 0.033

4.407 ± 0.03

5.962 ± 0.035

5.13 ± 0.028

6.774 ± 0.037

0.888 ± 0.014

3.38 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski