Amino acid dipepetide frequency for Vibrio phage 2E1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.769AlaAla: 6.769 ± 1.507
0.615AlaCys: 0.615 ± 0.241
5.011AlaAsp: 5.011 ± 0.738
4.044AlaGlu: 4.044 ± 0.714
2.198AlaPhe: 2.198 ± 0.474
5.274AlaGly: 5.274 ± 0.642
1.406AlaHis: 1.406 ± 0.383
5.802AlaIle: 5.802 ± 0.94
5.011AlaLys: 5.011 ± 0.91
6.153AlaLeu: 6.153 ± 0.755
3.34AlaMet: 3.34 ± 0.71
3.34AlaAsn: 3.34 ± 0.515
2.549AlaPro: 2.549 ± 0.477
3.252AlaGln: 3.252 ± 0.447
3.692AlaArg: 3.692 ± 0.514
5.098AlaSer: 5.098 ± 0.734
4.747AlaThr: 4.747 ± 1.052
4.659AlaVal: 4.659 ± 0.632
0.527AlaTrp: 0.527 ± 0.222
2.637AlaTyr: 2.637 ± 0.646
0.0AlaXaa: 0.0 ± 0.0
Cys
0.527CysAla: 0.527 ± 0.222
0.352CysCys: 0.352 ± 0.162
0.791CysAsp: 0.791 ± 0.333
1.846CysGlu: 1.846 ± 0.433
0.879CysPhe: 0.879 ± 0.288
1.055CysGly: 1.055 ± 0.331
0.176CysHis: 0.176 ± 0.113
0.791CysIle: 0.791 ± 0.267
1.319CysLys: 1.319 ± 0.391
0.703CysLeu: 0.703 ± 0.214
0.264CysMet: 0.264 ± 0.149
0.352CysAsn: 0.352 ± 0.177
0.527CysPro: 0.527 ± 0.219
0.703CysGln: 0.703 ± 0.27
0.615CysArg: 0.615 ± 0.245
1.143CysSer: 1.143 ± 0.371
0.703CysThr: 0.703 ± 0.298
0.791CysVal: 0.791 ± 0.349
0.176CysTrp: 0.176 ± 0.129
0.352CysTyr: 0.352 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
5.362AspAla: 5.362 ± 0.752
1.143AspCys: 1.143 ± 0.418
3.956AspAsp: 3.956 ± 0.537
5.274AspGlu: 5.274 ± 0.723
2.461AspPhe: 2.461 ± 0.36
5.626AspGly: 5.626 ± 0.816
1.67AspHis: 1.67 ± 0.365
3.78AspIle: 3.78 ± 0.604
4.307AspLys: 4.307 ± 0.61
4.132AspLeu: 4.132 ± 0.677
1.231AspMet: 1.231 ± 0.353
2.725AspAsn: 2.725 ± 0.482
1.582AspPro: 1.582 ± 0.453
1.846AspGln: 1.846 ± 0.368
2.813AspArg: 2.813 ± 0.45
3.868AspSer: 3.868 ± 0.626
3.34AspThr: 3.34 ± 0.5
4.307AspVal: 4.307 ± 0.607
1.143AspTrp: 1.143 ± 0.416
2.549AspTyr: 2.549 ± 0.459
0.0AspXaa: 0.0 ± 0.0
Glu
4.483GluAla: 4.483 ± 0.635
1.055GluCys: 1.055 ± 0.287
3.34GluAsp: 3.34 ± 0.492
4.395GluGlu: 4.395 ± 0.779
3.604GluPhe: 3.604 ± 0.653
3.692GluGly: 3.692 ± 0.532
1.231GluHis: 1.231 ± 0.399
4.044GluIle: 4.044 ± 0.552
4.044GluLys: 4.044 ± 0.679
7.384GluLeu: 7.384 ± 1.028
2.549GluMet: 2.549 ± 0.412
2.989GluAsn: 2.989 ± 0.546
1.67GluPro: 1.67 ± 0.329
2.373GluGln: 2.373 ± 0.416
3.077GluArg: 3.077 ± 0.444
6.153GluSer: 6.153 ± 0.713
2.901GluThr: 2.901 ± 0.51
3.956GluVal: 3.956 ± 0.553
1.406GluTrp: 1.406 ± 0.358
3.428GluTyr: 3.428 ± 0.486
0.0GluXaa: 0.0 ± 0.0
Phe
2.725PheAla: 2.725 ± 0.716
0.791PheCys: 0.791 ± 0.321
3.165PheAsp: 3.165 ± 0.489
3.428PheGlu: 3.428 ± 0.613
0.967PhePhe: 0.967 ± 0.305
3.252PheGly: 3.252 ± 0.8
0.352PheHis: 0.352 ± 0.16
2.286PheIle: 2.286 ± 0.407
2.989PheLys: 2.989 ± 0.518
2.637PheLeu: 2.637 ± 0.517
0.879PheMet: 0.879 ± 0.287
1.934PheAsn: 1.934 ± 0.44
0.967PhePro: 0.967 ± 0.334
1.494PheGln: 1.494 ± 0.372
1.934PheArg: 1.934 ± 0.4
2.373PheSer: 2.373 ± 0.353
2.725PheThr: 2.725 ± 0.517
2.373PheVal: 2.373 ± 0.476
0.527PheTrp: 0.527 ± 0.221
1.846PheTyr: 1.846 ± 0.407
0.0PheXaa: 0.0 ± 0.0
Gly
4.044GlyAla: 4.044 ± 0.658
1.143GlyCys: 1.143 ± 0.421
4.923GlyAsp: 4.923 ± 0.877
4.483GlyGlu: 4.483 ± 0.467
2.989GlyPhe: 2.989 ± 0.525
5.714GlyGly: 5.714 ± 0.822
0.703GlyHis: 0.703 ± 0.232
3.34GlyIle: 3.34 ± 0.513
4.835GlyLys: 4.835 ± 0.429
6.065GlyLeu: 6.065 ± 0.564
1.934GlyMet: 1.934 ± 0.482
3.956GlyAsn: 3.956 ± 0.657
0.879GlyPro: 0.879 ± 0.263
2.901GlyGln: 2.901 ± 0.461
2.022GlyArg: 2.022 ± 0.379
5.802GlySer: 5.802 ± 1.022
3.604GlyThr: 3.604 ± 0.824
4.307GlyVal: 4.307 ± 0.613
1.494GlyTrp: 1.494 ± 0.376
3.956GlyTyr: 3.956 ± 0.628
0.0GlyXaa: 0.0 ± 0.0
His
1.231HisAla: 1.231 ± 0.362
0.0HisCys: 0.0 ± 0.0
0.791HisAsp: 0.791 ± 0.313
0.879HisGlu: 0.879 ± 0.267
1.319HisPhe: 1.319 ± 0.348
1.055HisGly: 1.055 ± 0.406
0.703HisHis: 0.703 ± 0.274
1.406HisIle: 1.406 ± 0.351
1.582HisLys: 1.582 ± 0.44
1.67HisLeu: 1.67 ± 0.528
0.176HisMet: 0.176 ± 0.162
0.879HisAsn: 0.879 ± 0.286
0.703HisPro: 0.703 ± 0.288
0.615HisGln: 0.615 ± 0.244
1.231HisArg: 1.231 ± 0.314
1.319HisSer: 1.319 ± 0.403
0.791HisThr: 0.791 ± 0.275
1.143HisVal: 1.143 ± 0.295
0.176HisTrp: 0.176 ± 0.113
0.615HisTyr: 0.615 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
6.329IleAla: 6.329 ± 0.817
1.055IleCys: 1.055 ± 0.335
5.538IleAsp: 5.538 ± 0.616
5.362IleGlu: 5.362 ± 0.84
1.758IlePhe: 1.758 ± 0.438
3.428IleGly: 3.428 ± 0.812
0.967IleHis: 0.967 ± 0.297
3.692IleIle: 3.692 ± 0.685
4.923IleLys: 4.923 ± 0.589
3.428IleLeu: 3.428 ± 0.56
1.143IleMet: 1.143 ± 0.311
3.692IleAsn: 3.692 ± 0.507
2.11IlePro: 2.11 ± 0.443
2.198IleGln: 2.198 ± 0.377
2.11IleArg: 2.11 ± 0.37
4.395IleSer: 4.395 ± 0.628
4.571IleThr: 4.571 ± 0.616
3.516IleVal: 3.516 ± 0.517
0.44IleTrp: 0.44 ± 0.182
2.022IleTyr: 2.022 ± 0.535
0.0IleXaa: 0.0 ± 0.0
Lys
4.307LysAla: 4.307 ± 0.548
0.879LysCys: 0.879 ± 0.285
3.428LysAsp: 3.428 ± 0.511
3.78LysGlu: 3.78 ± 0.797
3.165LysPhe: 3.165 ± 0.494
3.604LysGly: 3.604 ± 0.47
1.231LysHis: 1.231 ± 0.344
4.483LysIle: 4.483 ± 0.464
5.45LysLys: 5.45 ± 0.786
6.153LysLeu: 6.153 ± 0.798
2.022LysMet: 2.022 ± 0.365
2.549LysAsn: 2.549 ± 0.429
2.813LysPro: 2.813 ± 0.473
3.077LysGln: 3.077 ± 0.594
4.044LysArg: 4.044 ± 0.599
4.044LysSer: 4.044 ± 0.719
4.132LysThr: 4.132 ± 0.61
5.274LysVal: 5.274 ± 0.661
0.879LysTrp: 0.879 ± 0.26
2.725LysTyr: 2.725 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
7.032LeuAla: 7.032 ± 0.998
1.406LeuCys: 1.406 ± 0.403
5.45LeuAsp: 5.45 ± 0.572
4.219LeuGlu: 4.219 ± 0.563
2.725LeuPhe: 2.725 ± 0.398
5.274LeuGly: 5.274 ± 1.138
1.846LeuHis: 1.846 ± 0.475
4.571LeuIle: 4.571 ± 0.764
5.45LeuLys: 5.45 ± 0.809
4.571LeuLeu: 4.571 ± 0.589
1.67LeuMet: 1.67 ± 0.404
5.626LeuAsn: 5.626 ± 0.643
2.373LeuPro: 2.373 ± 0.482
2.022LeuGln: 2.022 ± 0.373
3.428LeuArg: 3.428 ± 0.712
5.274LeuSer: 5.274 ± 0.853
5.538LeuThr: 5.538 ± 0.632
5.538LeuVal: 5.538 ± 0.591
0.791LeuTrp: 0.791 ± 0.274
2.022LeuTyr: 2.022 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
2.373MetAla: 2.373 ± 0.412
0.264MetCys: 0.264 ± 0.193
1.758MetAsp: 1.758 ± 0.379
1.055MetGlu: 1.055 ± 0.287
1.67MetPhe: 1.67 ± 0.388
1.582MetGly: 1.582 ± 0.404
0.352MetHis: 0.352 ± 0.192
2.286MetIle: 2.286 ± 0.526
1.67MetLys: 1.67 ± 0.406
1.67MetLeu: 1.67 ± 0.405
0.703MetMet: 0.703 ± 0.242
2.11MetAsn: 2.11 ± 0.401
0.967MetPro: 0.967 ± 0.346
1.055MetGln: 1.055 ± 0.357
1.846MetArg: 1.846 ± 0.429
3.165MetSer: 3.165 ± 0.576
2.11MetThr: 2.11 ± 0.378
2.022MetVal: 2.022 ± 0.459
0.44MetTrp: 0.44 ± 0.207
0.615MetTyr: 0.615 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
4.219AsnAla: 4.219 ± 0.802
0.703AsnCys: 0.703 ± 0.252
2.373AsnAsp: 2.373 ± 0.325
3.077AsnGlu: 3.077 ± 0.607
1.67AsnPhe: 1.67 ± 0.365
4.044AsnGly: 4.044 ± 0.583
1.143AsnHis: 1.143 ± 0.399
3.252AsnIle: 3.252 ± 0.621
3.604AsnLys: 3.604 ± 0.615
4.395AsnLeu: 4.395 ± 0.831
1.934AsnMet: 1.934 ± 0.338
3.165AsnAsn: 3.165 ± 0.495
2.989AsnPro: 2.989 ± 0.43
2.637AsnGln: 2.637 ± 0.423
1.406AsnArg: 1.406 ± 0.254
4.044AsnSer: 4.044 ± 0.672
2.989AsnThr: 2.989 ± 0.713
2.989AsnVal: 2.989 ± 0.446
0.615AsnTrp: 0.615 ± 0.241
2.198AsnTyr: 2.198 ± 0.41
0.0AsnXaa: 0.0 ± 0.0
Pro
2.11ProAla: 2.11 ± 0.468
0.352ProCys: 0.352 ± 0.179
2.637ProAsp: 2.637 ± 0.595
2.637ProGlu: 2.637 ± 0.5
1.319ProPhe: 1.319 ± 0.398
0.879ProGly: 0.879 ± 0.398
0.264ProHis: 0.264 ± 0.128
1.846ProIle: 1.846 ± 0.514
1.758ProLys: 1.758 ± 0.389
1.758ProLeu: 1.758 ± 0.363
0.879ProMet: 0.879 ± 0.316
1.231ProAsn: 1.231 ± 0.369
0.791ProPro: 0.791 ± 0.234
1.67ProGln: 1.67 ± 0.607
1.406ProArg: 1.406 ± 0.403
2.461ProSer: 2.461 ± 0.427
2.461ProThr: 2.461 ± 0.607
2.549ProVal: 2.549 ± 0.519
0.176ProTrp: 0.176 ± 0.124
1.406ProTyr: 1.406 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
3.252GlnAla: 3.252 ± 0.586
0.791GlnCys: 0.791 ± 0.253
1.846GlnAsp: 1.846 ± 0.361
2.022GlnGlu: 2.022 ± 0.393
2.022GlnPhe: 2.022 ± 0.402
3.34GlnGly: 3.34 ± 0.538
0.44GlnHis: 0.44 ± 0.236
2.989GlnIle: 2.989 ± 0.559
1.494GlnLys: 1.494 ± 0.3
2.901GlnLeu: 2.901 ± 0.472
1.319GlnMet: 1.319 ± 0.275
2.549GlnAsn: 2.549 ± 0.412
0.703GlnPro: 0.703 ± 0.325
2.901GlnGln: 2.901 ± 1.503
1.406GlnArg: 1.406 ± 0.323
2.725GlnSer: 2.725 ± 0.458
1.758GlnThr: 1.758 ± 0.35
2.461GlnVal: 2.461 ± 0.549
0.615GlnTrp: 0.615 ± 0.228
1.582GlnTyr: 1.582 ± 0.344
0.0GlnXaa: 0.0 ± 0.0
Arg
3.78ArgAla: 3.78 ± 0.628
0.615ArgCys: 0.615 ± 0.235
2.549ArgAsp: 2.549 ± 0.399
3.077ArgGlu: 3.077 ± 0.497
1.934ArgPhe: 1.934 ± 0.459
2.11ArgGly: 2.11 ± 0.448
0.703ArgHis: 0.703 ± 0.244
2.286ArgIle: 2.286 ± 0.407
2.286ArgLys: 2.286 ± 0.562
4.483ArgLeu: 4.483 ± 0.698
1.758ArgMet: 1.758 ± 0.471
2.11ArgAsn: 2.11 ± 0.337
0.703ArgPro: 0.703 ± 0.351
1.143ArgGln: 1.143 ± 0.384
1.582ArgArg: 1.582 ± 0.491
3.077ArgSer: 3.077 ± 0.568
1.934ArgThr: 1.934 ± 0.382
3.604ArgVal: 3.604 ± 0.494
0.615ArgTrp: 0.615 ± 0.221
1.231ArgTyr: 1.231 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
5.098SerAla: 5.098 ± 0.738
0.527SerCys: 0.527 ± 0.165
4.307SerAsp: 4.307 ± 0.592
4.307SerGlu: 4.307 ± 0.607
2.286SerPhe: 2.286 ± 0.409
6.241SerGly: 6.241 ± 0.961
1.406SerHis: 1.406 ± 0.445
5.362SerIle: 5.362 ± 0.685
5.89SerLys: 5.89 ± 0.813
4.659SerLeu: 4.659 ± 0.691
2.725SerMet: 2.725 ± 0.468
4.395SerAsn: 4.395 ± 0.48
2.198SerPro: 2.198 ± 0.459
2.813SerGln: 2.813 ± 0.473
2.286SerArg: 2.286 ± 0.449
4.923SerSer: 4.923 ± 0.902
5.098SerThr: 5.098 ± 0.921
4.923SerVal: 4.923 ± 0.813
1.143SerTrp: 1.143 ± 0.283
2.461SerTyr: 2.461 ± 0.535
0.0SerXaa: 0.0 ± 0.0
Thr
4.659ThrAla: 4.659 ± 0.931
0.527ThrCys: 0.527 ± 0.192
3.428ThrAsp: 3.428 ± 0.665
5.011ThrGlu: 5.011 ± 0.628
2.286ThrPhe: 2.286 ± 0.562
4.923ThrGly: 4.923 ± 0.631
1.143ThrHis: 1.143 ± 0.351
3.78ThrIle: 3.78 ± 0.508
3.868ThrLys: 3.868 ± 0.557
5.098ThrLeu: 5.098 ± 0.639
1.582ThrMet: 1.582 ± 0.556
2.373ThrAsn: 2.373 ± 0.449
3.077ThrPro: 3.077 ± 0.482
3.165ThrGln: 3.165 ± 0.544
1.582ThrArg: 1.582 ± 0.388
4.044ThrSer: 4.044 ± 0.538
4.132ThrThr: 4.132 ± 0.699
2.901ThrVal: 2.901 ± 0.556
0.615ThrTrp: 0.615 ± 0.214
2.373ThrTyr: 2.373 ± 0.672
0.0ThrXaa: 0.0 ± 0.0
Val
4.307ValAla: 4.307 ± 0.592
0.879ValCys: 0.879 ± 0.26
4.923ValAsp: 4.923 ± 0.635
5.186ValGlu: 5.186 ± 0.655
2.11ValPhe: 2.11 ± 0.507
4.219ValGly: 4.219 ± 0.752
1.143ValHis: 1.143 ± 0.321
3.868ValIle: 3.868 ± 0.699
4.835ValLys: 4.835 ± 0.684
4.044ValLeu: 4.044 ± 0.771
1.934ValMet: 1.934 ± 0.384
4.307ValAsn: 4.307 ± 0.471
1.406ValPro: 1.406 ± 0.338
1.055ValGln: 1.055 ± 0.324
2.461ValArg: 2.461 ± 0.46
5.89ValSer: 5.89 ± 0.66
4.307ValThr: 4.307 ± 0.63
4.483ValVal: 4.483 ± 0.752
0.352ValTrp: 0.352 ± 0.182
2.813ValTyr: 2.813 ± 0.482
0.0ValXaa: 0.0 ± 0.0
Trp
0.615TrpAla: 0.615 ± 0.201
0.088TrpCys: 0.088 ± 0.081
0.791TrpAsp: 0.791 ± 0.249
0.967TrpGlu: 0.967 ± 0.312
0.176TrpPhe: 0.176 ± 0.131
0.879TrpGly: 0.879 ± 0.296
0.44TrpHis: 0.44 ± 0.184
0.967TrpIle: 0.967 ± 0.272
0.967TrpLys: 0.967 ± 0.321
1.934TrpLeu: 1.934 ± 0.381
0.44TrpMet: 0.44 ± 0.168
0.527TrpAsn: 0.527 ± 0.225
0.615TrpPro: 0.615 ± 0.211
0.264TrpGln: 0.264 ± 0.143
1.055TrpArg: 1.055 ± 0.266
0.44TrpSer: 0.44 ± 0.208
0.264TrpThr: 0.264 ± 0.151
0.703TrpVal: 0.703 ± 0.273
0.264TrpTrp: 0.264 ± 0.166
0.527TrpTyr: 0.527 ± 0.233
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.901TyrAla: 2.901 ± 0.445
0.879TyrCys: 0.879 ± 0.347
2.11TyrAsp: 2.11 ± 0.442
2.725TyrGlu: 2.725 ± 0.548
2.11TyrPhe: 2.11 ± 0.486
2.901TyrGly: 2.901 ± 0.49
0.967TyrHis: 0.967 ± 0.285
1.846TyrIle: 1.846 ± 0.472
1.758TyrLys: 1.758 ± 0.361
2.989TyrLeu: 2.989 ± 0.39
1.143TyrMet: 1.143 ± 0.331
2.637TyrAsn: 2.637 ± 0.388
1.055TyrPro: 1.055 ± 0.312
1.934TyrGln: 1.934 ± 0.436
1.582TyrArg: 1.582 ± 0.257
2.813TyrSer: 2.813 ± 0.543
2.461TyrThr: 2.461 ± 0.323
2.022TyrVal: 2.022 ± 0.633
0.527TyrTrp: 0.527 ± 0.274
1.494TyrTyr: 1.494 ± 0.371
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (11377 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski