Sulfurimonas sp. UBA12504
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2274 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D3VWR2|A0A2D3VWR2_9PROT Flagellin_D0/D1 domain-containing protein OS=Sulfurimonas sp. UBA12504 OX=2015903 GN=CFH84_01480 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.4 LLKK5 pKa = 10.43 LSLAAAVLATGILAAEE21 pKa = 4.28 EE22 pKa = 4.37 SEE24 pKa = 4.13 IGISANVAATSNYY37 pKa = 6.57 VWRR40 pKa = 11.84 GMTQSADD47 pKa = 3.16 KK48 pKa = 10.22 PAIQGGFDD56 pKa = 3.73 LEE58 pKa = 4.46 YY59 pKa = 10.65 KK60 pKa = 10.34 GAYY63 pKa = 9.34 LGTWGSNVDD72 pKa = 3.48 WGNDD76 pKa = 2.95 ASMEE80 pKa = 4.12 ADD82 pKa = 4.96 FYY84 pKa = 11.68 AGYY87 pKa = 10.56 SNSLYY92 pKa = 10.7 GVTYY96 pKa = 10.32 DD97 pKa = 3.41 IGAIKK102 pKa = 9.87 IVYY105 pKa = 8.23 PGEE108 pKa = 4.1 MTSSNFAEE116 pKa = 4.83 VYY118 pKa = 10.53 LGLGYY123 pKa = 10.63 DD124 pKa = 3.93 FEE126 pKa = 4.92 IFSIGAKK133 pKa = 9.13 YY134 pKa = 10.91 SNGIKK139 pKa = 9.63 TDD141 pKa = 4.08 DD142 pKa = 3.98 FDD144 pKa = 6.68 APDD147 pKa = 3.64 NMEE150 pKa = 4.01 GTISVPLPVEE160 pKa = 3.94 ISLDD164 pKa = 3.36 AAYY167 pKa = 9.91 GVYY170 pKa = 10.61 GKK172 pKa = 10.31 DD173 pKa = 3.35 GDD175 pKa = 4.25 DD176 pKa = 3.63 NGVGKK181 pKa = 10.5 YY182 pKa = 9.74 YY183 pKa = 10.87 SVGLSKK189 pKa = 11.05 SFGKK193 pKa = 10.42 FDD195 pKa = 3.22 FTLAYY200 pKa = 9.31 TAMDD204 pKa = 4.49 YY205 pKa = 8.75 FTDD208 pKa = 4.59 GADD211 pKa = 3.67 SEE213 pKa = 5.21 DD214 pKa = 4.39 NIIGTIGTSFF224 pKa = 3.21
Molecular weight: 23.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.846
IPC_protein 3.846
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.973
Patrickios 0.515
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|A0A2D3VI56|A0A2D3VI56_9PROT RNA-binding protein OS=Sulfurimonas sp. UBA12504 OX=2015903 GN=CFH84_07940 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 6.05 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.26 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.64 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2274
0
2274
638461
26
1451
280.8
31.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.31 ± 0.059
0.898 ± 0.022
5.445 ± 0.038
7.195 ± 0.056
5.061 ± 0.048
5.713 ± 0.056
2.095 ± 0.029
8.19 ± 0.048
7.941 ± 0.057
10.017 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.719 ± 0.025
5.108 ± 0.04
2.859 ± 0.031
3.3 ± 0.029
3.418 ± 0.031
6.698 ± 0.041
5.139 ± 0.046
6.146 ± 0.043
0.751 ± 0.018
3.964 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here