Ralstonia phage Bakoly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bakolyvirus; Ralstonia virus Bakoly

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B8H5|A0A7G5B8H5_9CAUD Uncharacterized protein OS=Ralstonia phage Bakoly OX=2759709 GN=2B_00025 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.71TLLLDD7 pKa = 3.81TSTWDD12 pKa = 3.36LTTDD16 pKa = 3.19ASGNLATVGDD26 pKa = 4.47ADD28 pKa = 4.71RR29 pKa = 11.84DD30 pKa = 3.98ATNGGALRR38 pKa = 11.84LAQDD42 pKa = 3.25VAARR46 pKa = 11.84VSSWRR51 pKa = 11.84GEE53 pKa = 4.1VYY55 pKa = 10.89YY56 pKa = 9.65DD57 pKa = 3.35TTQGIPYY64 pKa = 8.49DD65 pKa = 4.38TILGEE70 pKa = 4.18YY71 pKa = 8.8PNLVVLQQLYY81 pKa = 10.57SEE83 pKa = 4.84EE84 pKa = 4.2SLNVPGCATALAEE97 pKa = 4.16LTYY100 pKa = 11.24DD101 pKa = 3.22RR102 pKa = 11.84AGRR105 pKa = 11.84LLGGTINVSDD115 pKa = 3.62ISGNPAVVTLL125 pKa = 4.35

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B8K3|A0A7G5B8K3_9CAUD Uncharacterized protein OS=Ralstonia phage Bakoly OX=2759709 GN=2B_00053 PE=4 SV=1
MM1 pKa = 7.73GIIKK5 pKa = 10.14LAIGSMFGSTEE16 pKa = 3.83QPSQIGTTKK25 pKa = 10.69RR26 pKa = 11.84NGGSTARR33 pKa = 11.84DD34 pKa = 2.92KK35 pKa = 11.08RR36 pKa = 11.84AARR39 pKa = 11.84KK40 pKa = 8.97ARR42 pKa = 11.84NVKK45 pKa = 8.49CHH47 pKa = 5.61KK48 pKa = 9.74RR49 pKa = 11.84ASKK52 pKa = 10.73

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13332

47

917

215.0

23.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.186 ± 0.521

0.923 ± 0.131

5.663 ± 0.269

5.258 ± 0.388

3.3 ± 0.192

8.333 ± 0.626

1.68 ± 0.169

4.298 ± 0.203

3.668 ± 0.305

8.303 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.175

3.615 ± 0.225

5.446 ± 0.239

4.193 ± 0.193

6.068 ± 0.351

5.566 ± 0.369

6.721 ± 0.504

7.036 ± 0.294

1.583 ± 0.148

2.858 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski