Amino acid dipepetide frequency for Ralstonia phage Bakoly

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.453AlaAla: 18.453 ± 1.361
0.9AlaCys: 0.9 ± 0.33
7.351AlaAsp: 7.351 ± 0.819
7.276AlaGlu: 7.276 ± 0.823
4.576AlaPhe: 4.576 ± 0.658
13.577AlaGly: 13.577 ± 2.126
1.725AlaHis: 1.725 ± 0.315
5.101AlaIle: 5.101 ± 0.579
3.826AlaLys: 3.826 ± 0.58
11.552AlaLeu: 11.552 ± 1.047
3.451AlaMet: 3.451 ± 0.481
4.726AlaAsn: 4.726 ± 0.723
6.601AlaPro: 6.601 ± 0.871
6.601AlaGln: 6.601 ± 0.681
9.152AlaArg: 9.152 ± 0.996
6.976AlaSer: 6.976 ± 0.732
7.876AlaThr: 7.876 ± 1.114
7.576AlaVal: 7.576 ± 0.615
1.125AlaTrp: 1.125 ± 0.325
3.376AlaTyr: 3.376 ± 0.6
0.0AlaXaa: 0.0 ± 0.0
Cys
1.05CysAla: 1.05 ± 0.229
0.225CysCys: 0.225 ± 0.134
0.9CysAsp: 0.9 ± 0.3
0.375CysGlu: 0.375 ± 0.167
0.0CysPhe: 0.0 ± 0.0
1.2CysGly: 1.2 ± 0.408
0.375CysHis: 0.375 ± 0.176
0.45CysIle: 0.45 ± 0.197
0.45CysLys: 0.45 ± 0.176
0.525CysLeu: 0.525 ± 0.274
0.075CysMet: 0.075 ± 0.077
0.3CysAsn: 0.3 ± 0.137
0.45CysPro: 0.45 ± 0.224
0.075CysGln: 0.075 ± 0.08
0.75CysArg: 0.75 ± 0.257
0.375CysSer: 0.375 ± 0.15
0.675CysThr: 0.675 ± 0.245
0.75CysVal: 0.75 ± 0.211
0.0CysTrp: 0.0 ± 0.0
0.225CysTyr: 0.225 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
7.951AspAla: 7.951 ± 0.68
0.675AspCys: 0.675 ± 0.23
3.676AspAsp: 3.676 ± 0.546
3.376AspGlu: 3.376 ± 0.579
1.8AspPhe: 1.8 ± 0.304
4.726AspGly: 4.726 ± 0.76
0.45AspHis: 0.45 ± 0.215
2.55AspIle: 2.55 ± 0.592
2.025AspLys: 2.025 ± 0.457
4.876AspLeu: 4.876 ± 0.536
1.575AspMet: 1.575 ± 0.387
1.8AspAsn: 1.8 ± 0.414
4.051AspPro: 4.051 ± 0.672
1.575AspGln: 1.575 ± 0.356
2.25AspArg: 2.25 ± 0.459
3.151AspSer: 3.151 ± 0.45
3.301AspThr: 3.301 ± 0.505
3.901AspVal: 3.901 ± 0.482
1.275AspTrp: 1.275 ± 0.283
1.65AspTyr: 1.65 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
7.501GluAla: 7.501 ± 1.109
0.45GluCys: 0.45 ± 0.234
2.175GluAsp: 2.175 ± 0.522
3.226GluGlu: 3.226 ± 0.583
1.725GluPhe: 1.725 ± 0.335
4.501GluGly: 4.501 ± 0.879
1.2GluHis: 1.2 ± 0.311
2.475GluIle: 2.475 ± 0.445
2.25GluLys: 2.25 ± 0.365
5.101GluLeu: 5.101 ± 0.616
1.05GluMet: 1.05 ± 0.267
2.025GluAsn: 2.025 ± 0.357
1.65GluPro: 1.65 ± 0.414
3.451GluGln: 3.451 ± 0.564
4.276GluArg: 4.276 ± 0.621
2.7GluSer: 2.7 ± 0.489
2.7GluThr: 2.7 ± 0.508
2.4GluVal: 2.4 ± 0.424
0.525GluTrp: 0.525 ± 0.198
1.2GluTyr: 1.2 ± 0.315
0.0GluXaa: 0.0 ± 0.0
Phe
4.201PheAla: 4.201 ± 0.571
0.6PheCys: 0.6 ± 0.221
2.775PheAsp: 2.775 ± 0.541
1.65PheGlu: 1.65 ± 0.351
1.125PhePhe: 1.125 ± 0.366
2.325PheGly: 2.325 ± 0.433
0.45PheHis: 0.45 ± 0.173
1.05PheIle: 1.05 ± 0.27
1.05PheLys: 1.05 ± 0.329
2.7PheLeu: 2.7 ± 0.542
0.6PheMet: 0.6 ± 0.24
1.65PheAsn: 1.65 ± 0.321
1.725PhePro: 1.725 ± 0.457
0.9PheGln: 0.9 ± 0.292
1.65PheArg: 1.65 ± 0.299
1.875PheSer: 1.875 ± 0.396
2.475PheThr: 2.475 ± 0.42
2.625PheVal: 2.625 ± 0.419
0.375PheTrp: 0.375 ± 0.158
1.2PheTyr: 1.2 ± 0.305
0.0PheXaa: 0.0 ± 0.0
Gly
11.252GlyAla: 11.252 ± 1.472
1.05GlyCys: 1.05 ± 0.384
3.451GlyAsp: 3.451 ± 0.438
4.876GlyGlu: 4.876 ± 1.175
4.051GlyPhe: 4.051 ± 0.58
7.951GlyGly: 7.951 ± 1.824
1.125GlyHis: 1.125 ± 0.286
4.276GlyIle: 4.276 ± 1.444
3.301GlyLys: 3.301 ± 0.456
6.301GlyLeu: 6.301 ± 0.657
1.275GlyMet: 1.275 ± 0.306
2.7GlyAsn: 2.7 ± 0.521
2.85GlyPro: 2.85 ± 0.525
2.625GlyGln: 2.625 ± 0.522
4.201GlyArg: 4.201 ± 0.6
3.976GlySer: 3.976 ± 0.763
5.926GlyThr: 5.926 ± 1.007
7.201GlyVal: 7.201 ± 0.635
2.025GlyTrp: 2.025 ± 0.505
2.926GlyTyr: 2.926 ± 0.589
0.0GlyXaa: 0.0 ± 0.0
His
2.25HisAla: 2.25 ± 0.351
0.15HisCys: 0.15 ± 0.113
1.05HisAsp: 1.05 ± 0.294
0.975HisGlu: 0.975 ± 0.329
0.3HisPhe: 0.3 ± 0.145
1.95HisGly: 1.95 ± 0.518
0.225HisHis: 0.225 ± 0.129
0.75HisIle: 0.75 ± 0.243
0.375HisLys: 0.375 ± 0.146
1.65HisLeu: 1.65 ± 0.298
0.225HisMet: 0.225 ± 0.119
0.75HisAsn: 0.75 ± 0.239
0.6HisPro: 0.6 ± 0.21
0.675HisGln: 0.675 ± 0.211
1.125HisArg: 1.125 ± 0.26
1.125HisSer: 1.125 ± 0.233
0.6HisThr: 0.6 ± 0.227
1.35HisVal: 1.35 ± 0.415
0.15HisTrp: 0.15 ± 0.103
0.525HisTyr: 0.525 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
5.551IleAla: 5.551 ± 0.672
0.225IleCys: 0.225 ± 0.148
2.926IleAsp: 2.926 ± 0.462
3.376IleGlu: 3.376 ± 0.797
1.35IlePhe: 1.35 ± 0.323
2.625IleGly: 2.625 ± 0.392
0.825IleHis: 0.825 ± 0.25
1.125IleIle: 1.125 ± 0.325
2.55IleLys: 2.55 ± 0.814
2.475IleLeu: 2.475 ± 0.541
0.975IleMet: 0.975 ± 0.307
1.425IleAsn: 1.425 ± 0.364
2.55IlePro: 2.55 ± 0.368
1.125IleGln: 1.125 ± 0.271
2.325IleArg: 2.325 ± 0.453
2.1IleSer: 2.1 ± 0.428
3.676IleThr: 3.676 ± 0.577
3.601IleVal: 3.601 ± 0.563
0.975IleTrp: 0.975 ± 0.24
1.2IleTyr: 1.2 ± 0.334
0.0IleXaa: 0.0 ± 0.0
Lys
4.426LysAla: 4.426 ± 0.8
0.225LysCys: 0.225 ± 0.128
1.575LysAsp: 1.575 ± 0.37
1.425LysGlu: 1.425 ± 0.275
1.05LysPhe: 1.05 ± 0.244
2.025LysGly: 2.025 ± 0.361
1.125LysHis: 1.125 ± 0.305
1.875LysIle: 1.875 ± 0.34
1.875LysLys: 1.875 ± 0.548
3.976LysLeu: 3.976 ± 0.501
0.9LysMet: 0.9 ± 0.27
1.05LysAsn: 1.05 ± 0.281
2.1LysPro: 2.1 ± 0.386
1.275LysGln: 1.275 ± 0.26
2.4LysArg: 2.4 ± 0.582
1.95LysSer: 1.95 ± 0.318
2.926LysThr: 2.926 ± 0.48
2.625LysVal: 2.625 ± 0.529
0.9LysTrp: 0.9 ± 0.264
0.975LysTyr: 0.975 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
10.352LeuAla: 10.352 ± 0.985
0.9LeuCys: 0.9 ± 0.32
5.626LeuAsp: 5.626 ± 0.605
3.976LeuGlu: 3.976 ± 0.641
2.025LeuPhe: 2.025 ± 0.443
5.626LeuGly: 5.626 ± 0.631
2.175LeuHis: 2.175 ± 0.553
3.001LeuIle: 3.001 ± 0.375
2.4LeuLys: 2.4 ± 0.435
6.301LeuLeu: 6.301 ± 0.819
1.875LeuMet: 1.875 ± 0.295
3.226LeuAsn: 3.226 ± 0.559
5.101LeuPro: 5.101 ± 0.658
3.301LeuGln: 3.301 ± 0.611
6.601LeuArg: 6.601 ± 0.906
5.251LeuSer: 5.251 ± 0.455
5.776LeuThr: 5.776 ± 0.988
6.226LeuVal: 6.226 ± 0.78
1.35LeuTrp: 1.35 ± 0.331
1.95LeuTyr: 1.95 ± 0.48
0.0LeuXaa: 0.0 ± 0.0
Met
3.151MetAla: 3.151 ± 0.483
0.075MetCys: 0.075 ± 0.077
0.825MetAsp: 0.825 ± 0.25
0.6MetGlu: 0.6 ± 0.21
0.45MetPhe: 0.45 ± 0.192
1.35MetGly: 1.35 ± 0.288
0.3MetHis: 0.3 ± 0.132
0.825MetIle: 0.825 ± 0.258
1.5MetLys: 1.5 ± 0.319
1.65MetLeu: 1.65 ± 0.356
0.45MetMet: 0.45 ± 0.195
0.6MetAsn: 0.6 ± 0.247
1.875MetPro: 1.875 ± 0.314
1.2MetGln: 1.2 ± 0.35
2.025MetArg: 2.025 ± 0.404
2.55MetSer: 2.55 ± 0.439
2.325MetThr: 2.325 ± 0.413
0.6MetVal: 0.6 ± 0.191
0.375MetTrp: 0.375 ± 0.152
0.3MetTyr: 0.3 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
4.951AsnAla: 4.951 ± 0.663
0.075AsnCys: 0.075 ± 0.059
2.1AsnAsp: 2.1 ± 0.418
1.725AsnGlu: 1.725 ± 0.404
1.2AsnPhe: 1.2 ± 0.338
2.775AsnGly: 2.775 ± 0.5
0.375AsnHis: 0.375 ± 0.153
1.8AsnIle: 1.8 ± 0.338
1.35AsnLys: 1.35 ± 0.303
2.25AsnLeu: 2.25 ± 0.381
1.275AsnMet: 1.275 ± 0.301
1.575AsnAsn: 1.575 ± 0.368
2.926AsnPro: 2.926 ± 0.557
0.6AsnGln: 0.6 ± 0.213
1.575AsnArg: 1.575 ± 0.34
2.4AsnSer: 2.4 ± 0.468
1.875AsnThr: 1.875 ± 0.386
2.625AsnVal: 2.625 ± 0.513
0.825AsnTrp: 0.825 ± 0.224
1.875AsnTyr: 1.875 ± 0.462
0.0AsnXaa: 0.0 ± 0.0
Pro
7.951ProAla: 7.951 ± 0.843
0.225ProCys: 0.225 ± 0.118
2.625ProAsp: 2.625 ± 0.453
3.376ProGlu: 3.376 ± 0.526
1.575ProPhe: 1.575 ± 0.408
5.401ProGly: 5.401 ± 0.603
0.825ProHis: 0.825 ± 0.254
1.875ProIle: 1.875 ± 0.349
2.175ProLys: 2.175 ± 0.469
3.676ProLeu: 3.676 ± 0.539
0.6ProMet: 0.6 ± 0.254
2.025ProAsn: 2.025 ± 0.311
3.376ProPro: 3.376 ± 0.598
2.55ProGln: 2.55 ± 0.416
1.8ProArg: 1.8 ± 0.336
3.226ProSer: 3.226 ± 0.471
5.251ProThr: 5.251 ± 0.918
3.826ProVal: 3.826 ± 0.448
0.525ProTrp: 0.525 ± 0.239
1.575ProTyr: 1.575 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
6.301GlnAla: 6.301 ± 0.655
0.075GlnCys: 0.075 ± 0.073
2.025GlnAsp: 2.025 ± 0.357
1.575GlnGlu: 1.575 ± 0.492
1.2GlnPhe: 1.2 ± 0.245
2.775GlnGly: 2.775 ± 0.463
0.975GlnHis: 0.975 ± 0.331
3.301GlnIle: 3.301 ± 0.469
1.65GlnLys: 1.65 ± 0.327
3.376GlnLeu: 3.376 ± 0.558
1.425GlnMet: 1.425 ± 0.351
1.5GlnAsn: 1.5 ± 0.343
2.4GlnPro: 2.4 ± 0.333
2.775GlnGln: 2.775 ± 0.62
2.475GlnArg: 2.475 ± 0.434
2.25GlnSer: 2.25 ± 0.382
2.4GlnThr: 2.4 ± 0.449
2.175GlnVal: 2.175 ± 0.417
0.45GlnTrp: 0.45 ± 0.217
0.825GlnTyr: 0.825 ± 0.257
0.0GlnXaa: 0.0 ± 0.0
Arg
8.551ArgAla: 8.551 ± 0.93
0.9ArgCys: 0.9 ± 0.265
4.426ArgAsp: 4.426 ± 0.628
3.901ArgGlu: 3.901 ± 0.594
2.25ArgPhe: 2.25 ± 0.408
3.601ArgGly: 3.601 ± 0.572
1.2ArgHis: 1.2 ± 0.31
2.025ArgIle: 2.025 ± 0.382
2.175ArgLys: 2.175 ± 0.452
4.876ArgLeu: 4.876 ± 0.54
1.875ArgMet: 1.875 ± 0.352
1.875ArgAsn: 1.875 ± 0.387
3.226ArgPro: 3.226 ± 0.501
3.001ArgGln: 3.001 ± 0.478
3.076ArgArg: 3.076 ± 0.544
1.8ArgSer: 1.8 ± 0.48
3.076ArgThr: 3.076 ± 0.483
5.176ArgVal: 5.176 ± 0.701
1.425ArgTrp: 1.425 ± 0.28
2.25ArgTyr: 2.25 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
6.676SerAla: 6.676 ± 0.727
0.45SerCys: 0.45 ± 0.183
3.076SerAsp: 3.076 ± 0.519
2.7SerGlu: 2.7 ± 0.501
2.1SerPhe: 2.1 ± 0.355
6.376SerGly: 6.376 ± 0.994
0.375SerHis: 0.375 ± 0.185
2.85SerIle: 2.85 ± 0.463
1.8SerLys: 1.8 ± 0.448
4.576SerLeu: 4.576 ± 0.584
1.575SerMet: 1.575 ± 0.305
2.1SerAsn: 2.1 ± 0.38
2.325SerPro: 2.325 ± 0.386
2.4SerGln: 2.4 ± 0.385
2.775SerArg: 2.775 ± 0.422
3.301SerSer: 3.301 ± 0.599
3.751SerThr: 3.751 ± 0.51
4.126SerVal: 4.126 ± 0.501
0.75SerTrp: 0.75 ± 0.244
1.575SerTyr: 1.575 ± 0.38
0.0SerXaa: 0.0 ± 0.0
Thr
7.351ThrAla: 7.351 ± 0.791
0.375ThrCys: 0.375 ± 0.134
3.301ThrAsp: 3.301 ± 0.495
2.7ThrGlu: 2.7 ± 0.516
2.25ThrPhe: 2.25 ± 0.391
5.926ThrGly: 5.926 ± 1.031
0.675ThrHis: 0.675 ± 0.273
3.901ThrIle: 3.901 ± 0.592
2.4ThrLys: 2.4 ± 0.39
5.326ThrLeu: 5.326 ± 0.526
1.425ThrMet: 1.425 ± 0.25
2.55ThrAsn: 2.55 ± 0.445
4.651ThrPro: 4.651 ± 0.668
2.7ThrGln: 2.7 ± 0.524
4.576ThrArg: 4.576 ± 0.621
3.601ThrSer: 3.601 ± 0.683
5.401ThrThr: 5.401 ± 0.997
5.026ThrVal: 5.026 ± 0.768
1.05ThrTrp: 1.05 ± 0.306
2.025ThrTyr: 2.025 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
8.476ValAla: 8.476 ± 0.638
0.75ValCys: 0.75 ± 0.241
4.126ValAsp: 4.126 ± 0.637
3.676ValGlu: 3.676 ± 0.525
2.475ValPhe: 2.475 ± 0.431
4.576ValGly: 4.576 ± 0.529
1.275ValHis: 1.275 ± 0.272
2.25ValIle: 2.25 ± 0.355
2.55ValLys: 2.55 ± 0.415
7.876ValLeu: 7.876 ± 0.606
1.5ValMet: 1.5 ± 0.376
2.25ValAsn: 2.25 ± 0.461
4.126ValPro: 4.126 ± 0.574
3.226ValGln: 3.226 ± 0.57
3.826ValArg: 3.826 ± 0.476
4.201ValSer: 4.201 ± 0.702
4.426ValThr: 4.426 ± 0.782
5.851ValVal: 5.851 ± 0.662
1.05ValTrp: 1.05 ± 0.287
1.875ValTyr: 1.875 ± 0.367
0.0ValXaa: 0.0 ± 0.0
Trp
1.575TrpAla: 1.575 ± 0.427
0.45TrpCys: 0.45 ± 0.171
0.6TrpAsp: 0.6 ± 0.219
0.6TrpGlu: 0.6 ± 0.229
0.675TrpPhe: 0.675 ± 0.216
1.5TrpGly: 1.5 ± 0.446
0.525TrpHis: 0.525 ± 0.26
0.375TrpIle: 0.375 ± 0.16
0.3TrpLys: 0.3 ± 0.161
1.65TrpLeu: 1.65 ± 0.358
0.3TrpMet: 0.3 ± 0.136
0.45TrpAsn: 0.45 ± 0.191
0.45TrpPro: 0.45 ± 0.192
0.75TrpGln: 0.75 ± 0.259
1.725TrpArg: 1.725 ± 0.367
1.05TrpSer: 1.05 ± 0.389
0.975TrpThr: 0.975 ± 0.368
1.05TrpVal: 1.05 ± 0.298
0.375TrpTrp: 0.375 ± 0.155
0.45TrpTyr: 0.45 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.901TyrAla: 3.901 ± 0.472
0.45TyrCys: 0.45 ± 0.166
2.025TyrAsp: 2.025 ± 0.451
1.2TyrGlu: 1.2 ± 0.247
0.825TyrPhe: 0.825 ± 0.252
2.55TyrGly: 2.55 ± 0.459
0.6TyrHis: 0.6 ± 0.242
1.125TyrIle: 1.125 ± 0.288
0.675TyrLys: 0.675 ± 0.252
2.325TyrLeu: 2.325 ± 0.381
0.525TyrMet: 0.525 ± 0.212
1.575TyrAsn: 1.575 ± 0.264
1.425TyrPro: 1.425 ± 0.318
1.125TyrGln: 1.125 ± 0.26
2.1TyrArg: 2.1 ± 0.555
1.8TyrSer: 1.8 ± 0.351
1.8TyrThr: 1.8 ± 0.375
1.65TyrVal: 1.65 ± 0.314
0.3TyrTrp: 0.3 ± 0.141
0.6TyrTyr: 0.6 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski