Kangiella koreensis (strain DSM 16069 / KCTC 12182 / SW-125)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Kangiellaceae; Kangiella; Kangiella koreensis

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2630 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7RB18|C7RB18_KANKD Helicase c2 OS=Kangiella koreensis (strain DSM 16069 / KCTC 12182 / SW-125) OX=523791 GN=Kkor_1041 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.36KK3 pKa = 10.32VIPFLASALAFLPQSVISQDD23 pKa = 3.6TILVDD28 pKa = 3.12TGEE31 pKa = 4.59VYY33 pKa = 10.94GHH35 pKa = 6.17GPYY38 pKa = 10.5YY39 pKa = 10.62GVEE42 pKa = 4.13AYY44 pKa = 10.56GNASYY49 pKa = 11.76VNDD52 pKa = 3.92GSKK55 pKa = 10.15EE56 pKa = 3.79IKK58 pKa = 10.76VFDD61 pKa = 3.91VSDD64 pKa = 3.69ASNITLSGEE73 pKa = 4.28VEE75 pKa = 4.43VGCTVDD81 pKa = 6.3DD82 pKa = 4.88LDD84 pKa = 6.25LDD86 pKa = 4.2GATLVAKK93 pKa = 10.36CPFEE97 pKa = 3.55IHH99 pKa = 6.81FYY101 pKa = 11.4DD102 pKa = 4.66LTDD105 pKa = 3.43SLNPEE110 pKa = 4.2LVGSYY115 pKa = 10.82DD116 pKa = 3.52STSYY120 pKa = 11.18AVNSVEE126 pKa = 4.59LSGDD130 pKa = 3.08RR131 pKa = 11.84LYY133 pKa = 10.63IGGGNTDD140 pKa = 2.58IVIFNASDD148 pKa = 3.57LSNIQEE154 pKa = 4.47LNSQTFDD161 pKa = 3.64SISTGEE167 pKa = 4.21LKK169 pKa = 10.65KK170 pKa = 10.76SANFVYY176 pKa = 10.45SISDD180 pKa = 3.54FDD182 pKa = 3.91TVKK185 pKa = 10.59IFDD188 pKa = 3.67VSNEE192 pKa = 3.68EE193 pKa = 4.35NIIKK197 pKa = 9.71TSEE200 pKa = 3.79INATATLFKK209 pKa = 10.8DD210 pKa = 3.42VLIIDD215 pKa = 3.64NTLYY219 pKa = 10.41IAATDD224 pKa = 3.67GLRR227 pKa = 11.84VYY229 pKa = 10.63DD230 pKa = 4.12VTNMASPSYY239 pKa = 11.0VKK241 pKa = 10.08TIDD244 pKa = 3.17SGSFFNTIDD253 pKa = 4.02LLNALHH259 pKa = 6.45VVGDD263 pKa = 3.49TLYY266 pKa = 10.89AGKK269 pKa = 10.46SNAWIFEE276 pKa = 3.89FDD278 pKa = 3.02ITTPLDD284 pKa = 3.49PAFLGQFRR292 pKa = 11.84YY293 pKa = 8.49STGTTNQITDD303 pKa = 3.09NGTNMFIAFGIDD315 pKa = 3.2GLATAEE321 pKa = 4.14MQPMDD326 pKa = 4.37KK327 pKa = 10.26LDD329 pKa = 3.97HH330 pKa = 6.39FLVSILPNDD339 pKa = 3.68LSMDD343 pKa = 3.56NGRR346 pKa = 11.84LLASDD351 pKa = 3.58EE352 pKa = 4.33TGLFHH357 pKa = 7.9VIDD360 pKa = 4.24VEE362 pKa = 4.24NSFIPKK368 pKa = 9.88SRR370 pKa = 11.84IPYY373 pKa = 9.49ISNTFSGEE381 pKa = 3.89IKK383 pKa = 11.07NNTAWLGTGIYY394 pKa = 10.37LEE396 pKa = 4.85TIDD399 pKa = 6.22LSTLNPSSQIDD410 pKa = 3.65IQTPDD415 pKa = 2.93ASAEE419 pKa = 3.93VTYY422 pKa = 10.86LNEE425 pKa = 4.97IGDD428 pKa = 4.14TLYY431 pKa = 11.24LGTSSGMVAMYY442 pKa = 9.96DD443 pKa = 3.3VSGNVPSLIASITFPVHH460 pKa = 7.09AEE462 pKa = 4.22TNSPQYY468 pKa = 9.3ITDD471 pKa = 3.48VVPYY475 pKa = 10.4GDD477 pKa = 4.17YY478 pKa = 11.3LLASSIEE485 pKa = 4.15SEE487 pKa = 4.33LLIADD492 pKa = 4.77FSNLASPAVVNVPEE506 pKa = 4.22LSEE509 pKa = 3.96VAEE512 pKa = 4.4ANLFVTGDD520 pKa = 3.77KK521 pKa = 10.8LLSASDD527 pKa = 3.13WGAFLIDD534 pKa = 3.99ISDD537 pKa = 3.89IEE539 pKa = 4.22NPTYY543 pKa = 10.89YY544 pKa = 9.68GTQLVEE550 pKa = 4.34LGLITSATRR559 pKa = 11.84LDD561 pKa = 3.84ADD563 pKa = 3.64QVLLSSTEE571 pKa = 3.84GLLTVNIADD580 pKa = 4.65PDD582 pKa = 4.14NITIVSKK589 pKa = 10.86IEE591 pKa = 3.9SAPEE595 pKa = 3.81FSSVATDD602 pKa = 4.13GSHH605 pKa = 6.07VVGALHH611 pKa = 6.64YY612 pKa = 9.88EE613 pKa = 4.38SEE615 pKa = 4.21LKK617 pKa = 10.5LYY619 pKa = 10.36KK620 pKa = 10.2FNKK623 pKa = 10.14SPTTSDD629 pKa = 3.54YY630 pKa = 11.16EE631 pKa = 4.17FSVDD635 pKa = 3.37EE636 pKa = 4.72DD637 pKa = 4.18SSIVEE642 pKa = 4.26YY643 pKa = 11.4VEE645 pKa = 4.62ATDD648 pKa = 3.98PDD650 pKa = 3.62GDD652 pKa = 4.09EE653 pKa = 4.05IVFSIVSQPDD663 pKa = 3.27SGSVSITSAGQFTYY677 pKa = 10.79EE678 pKa = 4.36PNADD682 pKa = 3.81FNGQDD687 pKa = 2.88TFTFEE692 pKa = 5.73AEE694 pKa = 3.96DD695 pKa = 4.21TYY697 pKa = 11.62GGSSEE702 pKa = 4.22GSVSITINSVNDD714 pKa = 3.28APTASSVSLTTTVDD728 pKa = 3.27KK729 pKa = 11.08AVSGSFNASDD739 pKa = 3.53VDD741 pKa = 3.96GDD743 pKa = 3.69EE744 pKa = 4.16LTYY747 pKa = 11.28EE748 pKa = 4.11NTNPSNGSLSVSGSGFTYY766 pKa = 9.99TPAAGFSGQDD776 pKa = 2.99SFQYY780 pKa = 10.43TVTDD784 pKa = 3.25SGGEE788 pKa = 4.16SVTANVTITVNKK800 pKa = 8.43PASSGGGGGSSDD812 pKa = 3.67LWLLALLLLGFTFRR826 pKa = 11.84RR827 pKa = 11.84HH828 pKa = 4.52IRR830 pKa = 3.38

Molecular weight: 88.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7RAG2|C7RAG2_KANKD Uncharacterized protein OS=Kangiella koreensis (strain DSM 16069 / KCTC 12182 / SW-125) OX=523791 GN=Kkor_0834 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSIIKK11 pKa = 10.36RR12 pKa = 11.84KK13 pKa = 7.45RR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84QVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2630

0

2630

852378

30

2213

324.1

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.079 ± 0.048

0.89 ± 0.014

5.778 ± 0.037

6.798 ± 0.052

4.187 ± 0.033

6.815 ± 0.044

2.236 ± 0.025

6.621 ± 0.044

5.745 ± 0.04

10.195 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.027

4.264 ± 0.036

3.941 ± 0.031

4.581 ± 0.042

4.442 ± 0.036

6.551 ± 0.041

5.082 ± 0.031

6.754 ± 0.034

1.301 ± 0.019

3.243 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski