Vibrio phage vB_VchM-138

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9C150|H9C150_9CAUD Uncharacterized protein OS=Vibrio phage vB_VchM-138 OX=1127518 GN=VchM-138_0067 PE=4 SV=1
MM1 pKa = 7.68IDD3 pKa = 3.45VLFVALVAVAIYY15 pKa = 10.87NPTLTLWILVSMVVVSDD32 pKa = 3.93ALFGAVDD39 pKa = 3.48GWVYY43 pKa = 10.46FGLMALIDD51 pKa = 4.3LLCDD55 pKa = 5.2RR56 pKa = 11.84ITPHH60 pKa = 5.44NWGVIIIQDD69 pKa = 4.03PFPYY73 pKa = 9.86EE74 pKa = 4.21CNRR77 pKa = 11.84GLSTT81 pKa = 4.63

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9C0Y8|H9C0Y8_9CAUD Uncharacterized protein OS=Vibrio phage vB_VchM-138 OX=1127518 GN=VchM-138_0005 PE=4 SV=1
MM1 pKa = 7.1FQRR4 pKa = 11.84IIASLMAQANHH15 pKa = 5.95TAANSIGNQLARR27 pKa = 11.84PVSDD31 pKa = 3.6NAWGNRR37 pKa = 11.84KK38 pKa = 9.46GGRR41 pKa = 11.84KK42 pKa = 7.17QAHH45 pKa = 6.34RR46 pKa = 11.84FSGVAAQRR54 pKa = 11.84RR55 pKa = 11.84AARR58 pKa = 11.84KK59 pKa = 8.91IRR61 pKa = 11.84NQRR64 pKa = 11.84KK65 pKa = 9.16SKK67 pKa = 10.81

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13960

39

826

208.4

23.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.865 ± 0.289

1.34 ± 0.139

6.862 ± 0.189

5.487 ± 0.186

3.632 ± 0.224

7.092 ± 0.259

1.819 ± 0.156

6.533 ± 0.266

5.143 ± 0.236

7.615 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.129

5.365 ± 0.244

4.42 ± 0.324

3.797 ± 0.213

5.745 ± 0.25

6.304 ± 0.211

6.533 ± 0.261

6.884 ± 0.244

1.418 ± 0.116

3.546 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski