Arenibacter nanhaiticus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3553 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6I4C6|A0A1M6I4C6_9FLAO Glycerol kinase OS=Arenibacter nanhaiticus OX=558155 GN=glpK PE=3 SV=1
MM1 pKa = 7.18 EE2 pKa = 5.06 HH3 pKa = 6.91 SISIFAFKK11 pKa = 10.44 KK12 pKa = 9.57 SSCSGTLGKK21 pKa = 10.68 LFFLGFFLLTSLSFAQQADD40 pKa = 3.79 LRR42 pKa = 11.84 SHH44 pKa = 4.52 VTEE47 pKa = 4.79 GQDD50 pKa = 3.28 CTSNDD55 pKa = 3.21 VQLISAQLSSEE66 pKa = 4.05 EE67 pKa = 4.55 CTTCSPGDD75 pKa = 3.64 ILVVDD80 pKa = 4.26 LLITVRR86 pKa = 11.84 HH87 pKa = 5.12 NTNSVRR93 pKa = 11.84 PSLAVFGDD101 pKa = 4.24 LTTTLPDD108 pKa = 3.78 GSSTTSVFTEE118 pKa = 4.22 CGGPIIPKK126 pKa = 10.31 DD127 pKa = 3.41 KK128 pKa = 10.41 VLNGIPVDD136 pKa = 3.4 KK137 pKa = 10.43 GGNGIQTIKK146 pKa = 10.22 YY147 pKa = 7.94 GQVSYY152 pKa = 10.95 ICGSKK157 pKa = 11.03 LEE159 pKa = 4.02 VDD161 pKa = 4.45 NILVAWTVPPDD172 pKa = 4.12 GGCPIADD179 pKa = 3.89 ASPHH183 pKa = 5.99 PKK185 pKa = 10.21 CGFQEE190 pKa = 4.52 GSLIIIPPLHH200 pKa = 6.91 AIAEE204 pKa = 4.41 AFCTPEE210 pKa = 3.78 NYY212 pKa = 9.38 IDD214 pKa = 4.19 LEE216 pKa = 4.72 AIGGSAPYY224 pKa = 9.69 TYY226 pKa = 10.54 DD227 pKa = 2.71 WKK229 pKa = 11.13 GPNGFTSTDD238 pKa = 3.08 EE239 pKa = 4.44 DD240 pKa = 4.51 LFGVLPGNYY249 pKa = 9.29 DD250 pKa = 3.46 VTVTDD255 pKa = 5.25 SKK257 pKa = 11.33 GCSINTSVTKK267 pKa = 10.52 IKK269 pKa = 10.5 CCEE272 pKa = 3.94 FFATPNLDD280 pKa = 3.17 SSEE283 pKa = 4.03 QNIEE287 pKa = 3.95 GCDD290 pKa = 3.78 VSDD293 pKa = 4.52 LPSPFTDD300 pKa = 3.58 PEE302 pKa = 4.31 NVFTDD307 pKa = 3.28 ITEE310 pKa = 4.33 NPCGNLIMHH319 pKa = 6.68 QSDD322 pKa = 3.38 ILNGNLCTGGLIINRR337 pKa = 11.84 TYY339 pKa = 11.2 TLFDD343 pKa = 4.46 DD344 pKa = 5.12 LNNNNTLDD352 pKa = 3.68 SGEE355 pKa = 4.23 EE356 pKa = 3.93 FAVSYY361 pKa = 10.7 QNFRR365 pKa = 11.84 IIDD368 pKa = 3.63 TTLPTFNTPLPEE380 pKa = 5.17 DD381 pKa = 3.42 STAAFDD387 pKa = 5.21 NIPSPPTLSANDD399 pKa = 3.55 NCDD402 pKa = 3.79 DD403 pKa = 4.03 NVSVTLVEE411 pKa = 4.38 TYY413 pKa = 10.37 IGDD416 pKa = 4.07 STSTTYY422 pKa = 10.61 TIVRR426 pKa = 11.84 TWTASDD432 pKa = 3.68 CAGNEE437 pKa = 4.35 TVHH440 pKa = 5.34 TQYY443 pKa = 10.95 IYY445 pKa = 9.79 VTEE448 pKa = 4.34 NGDD451 pKa = 4.23 PIGLKK456 pKa = 10.35 INDD459 pKa = 3.69 VTVDD463 pKa = 3.47 EE464 pKa = 5.08 ADD466 pKa = 3.28 NTAVFTVTLTGRR478 pKa = 11.84 VSGGFNVDD486 pKa = 3.65 YY487 pKa = 11.36 NSVNQTAIAPNDD499 pKa = 3.4 FTAIGITSLSFSGNHH514 pKa = 5.81 GEE516 pKa = 4.3 KK517 pKa = 9.17 KK518 pKa = 9.17 TISVSIKK525 pKa = 9.3 EE526 pKa = 4.17 DD527 pKa = 3.24 QFVEE531 pKa = 4.08 DD532 pKa = 5.52 DD533 pKa = 3.55 EE534 pKa = 4.98 TFTIDD539 pKa = 5.04 LSNLSTTEE547 pKa = 3.51 IPINKK552 pKa = 8.49 ATGTGTIIDD561 pKa = 4.32 NDD563 pKa = 3.92 SATISITDD571 pKa = 3.43 MEE573 pKa = 4.84 INEE576 pKa = 4.22 GTGIFNIQVTLIGKK590 pKa = 6.38 TQDD593 pKa = 2.98 PFTINFSTADD603 pKa = 3.37 GSAIKK608 pKa = 10.59 VDD610 pKa = 4.58 DD611 pKa = 3.82 YY612 pKa = 12.01 VEE614 pKa = 5.17 AIGQITFPANSTNGTVQNISLEE636 pKa = 4.34 VIDD639 pKa = 5.5 DD640 pKa = 4.31 NIIEE644 pKa = 4.26 FTEE647 pKa = 4.01 NFLVNLNSISGFGDD661 pKa = 2.87 ISFLDD666 pKa = 3.53 NQANIDD672 pKa = 4.31 IIDD675 pKa = 3.76 NDD677 pKa = 4.88 AITGTGISFDD687 pKa = 3.63 NTNVIVNEE695 pKa = 4.23 DD696 pKa = 3.32 AGTAIFTVRR705 pKa = 11.84 LTGQVFGGFTLDD717 pKa = 3.54 YY718 pKa = 8.29 TTEE721 pKa = 4.11 NVTAMAGSDD730 pKa = 3.47 YY731 pKa = 10.31 TAEE734 pKa = 4.03 SGKK737 pKa = 10.94 LNFAGNDD744 pKa = 3.64 NEE746 pKa = 4.08 FHH748 pKa = 6.45 TIEE751 pKa = 4.52 VPIIDD756 pKa = 4.45 DD757 pKa = 3.69 TLIEE761 pKa = 4.14 PTEE764 pKa = 4.15 TFVVNLSNIDD774 pKa = 3.64 PAIVSINTAQARR786 pKa = 11.84 GTIKK790 pKa = 11.15 DD791 pKa = 3.48 NDD793 pKa = 3.54 ATAGTGVSFKK803 pKa = 10.4 NTDD806 pKa = 2.61 ITVNEE811 pKa = 4.38 DD812 pKa = 2.66 AGTATFKK819 pKa = 11.02 VVLSGNVQGGFTLDD833 pKa = 3.46 YY834 pKa = 8.43 TTDD837 pKa = 3.31 NGSAVQPDD845 pKa = 5.42 DD846 pKa = 3.41 YY847 pKa = 11.33 TLTYY851 pKa = 9.08 DD852 pKa = 3.25 TLTFVGNDD860 pKa = 3.79 GEE862 pKa = 4.75 TKK864 pKa = 10.52 DD865 pKa = 3.37 ITVPIIDD872 pKa = 5.17 DD873 pKa = 3.97 LLIEE877 pKa = 4.11 PTEE880 pKa = 3.96 NFFVKK885 pKa = 10.59 LSNLSTSLIGINTPQAKK902 pKa = 10.32 GNIIDD907 pKa = 3.93 NDD909 pKa = 4.38 AIAGTGIAFTNTNVTVTEE927 pKa = 4.22 GTDD930 pKa = 2.75 AFAIFEE936 pKa = 4.33 VTLTGDD942 pKa = 2.61 ISEE945 pKa = 4.57 NVSVDD950 pKa = 3.13 YY951 pKa = 8.25 TTIDD955 pKa = 3.3 GTALDD960 pKa = 4.3 PSDD963 pKa = 4.27 YY964 pKa = 10.57 LTTTSTITFTPTIKK978 pKa = 10.32 SYY980 pKa = 10.36 EE981 pKa = 4.0 IQVPIMDD988 pKa = 4.72 DD989 pKa = 3.97 AIIEE993 pKa = 4.13 PSEE996 pKa = 4.08 AFTVEE1001 pKa = 4.12 LSNIQSNLGIGFVDD1015 pKa = 4.74 GNATNTATGNILDD1028 pKa = 4.78 DD1029 pKa = 4.54 DD1030 pKa = 4.55 AVGGTGIAFTYY1041 pKa = 10.23 TNVTVTEE1048 pKa = 4.24 GTDD1051 pKa = 2.99 AFAA1054 pKa = 6.04
Molecular weight: 112.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.63
IPC_protein 3.694
Toseland 3.452
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.363
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.05
Thurlkill 3.503
EMBOSS 3.63
Sillero 3.808
Patrickios 1.329
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A1M6A0A3|A0A1M6A0A3_9FLAO Glutamate--tRNA ligase OS=Arenibacter nanhaiticus OX=558155 GN=gltX PE=3 SV=1
MM1 pKa = 7.04 TMYY4 pKa = 8.87 RR5 pKa = 11.84 TSYY8 pKa = 10.4 EE9 pKa = 3.91 VAQVLEE15 pKa = 4.38 RR16 pKa = 11.84 NRR18 pKa = 11.84 EE19 pKa = 3.89 QLAGLCANSWQSRR32 pKa = 11.84 TLHH35 pKa = 6.62 ALRR38 pKa = 11.84 KK39 pKa = 9.5 CRR41 pKa = 11.84 TAALGGHH48 pKa = 7.01 IDD50 pKa = 3.39 RR51 pKa = 11.84 CDD53 pKa = 3.33 NPGCHH58 pKa = 6.95 KK59 pKa = 10.79 LHH61 pKa = 6.88 LSYY64 pKa = 11.02 NSCRR68 pKa = 11.84 NRR70 pKa = 11.84 HH71 pKa = 5.34 CPKK74 pKa = 10.24 CQGHH78 pKa = 6.77 KK79 pKa = 9.45 RR80 pKa = 11.84 EE81 pKa = 3.69 QWIRR85 pKa = 11.84 ARR87 pKa = 11.84 EE88 pKa = 4.01 AEE90 pKa = 4.5 LLNVTYY96 pKa = 10.04 FHH98 pKa = 6.75 MVFTLPSEE106 pKa = 4.28 LNRR109 pKa = 11.84 MCLYY113 pKa = 10.38 DD114 pKa = 3.75 PKK116 pKa = 10.77 SIYY119 pKa = 10.89 GLLFGTAWQVIKK131 pKa = 10.87 GFSSNPKK138 pKa = 10.02 FLGADD143 pKa = 3.32 PGMVAILHH151 pKa = 6.06 SWGQNLSLHH160 pKa = 6.08 PHH162 pKa = 5.95 LHH164 pKa = 6.6 CIVPGGGTTRR174 pKa = 11.84 SGKK177 pKa = 9.11 WKK179 pKa = 9.77 FARR182 pKa = 11.84 NKK184 pKa = 9.96 GKK186 pKa = 9.61 PGCRR190 pKa = 11.84 KK191 pKa = 9.43 ARR193 pKa = 11.84 YY194 pKa = 8.26 LFPVKK199 pKa = 10.55 AMSKK203 pKa = 8.25 VFRR206 pKa = 11.84 ARR208 pKa = 11.84 FVASLRR214 pKa = 11.84 KK215 pKa = 9.22 EE216 pKa = 4.31 FPPHH220 pKa = 5.26 PTAFYY225 pKa = 11.01 EE226 pKa = 4.95 NLFKK230 pKa = 11.12 HH231 pKa = 5.64 NWVIYY236 pKa = 8.98 CKK238 pKa = 10.32 RR239 pKa = 11.84 PFLGPEE245 pKa = 3.69 QVVEE249 pKa = 3.68 YY250 pKa = 10.34 LGRR253 pKa = 11.84 YY254 pKa = 3.88 THH256 pKa = 7.36 KK257 pKa = 10.47 IAISNHH263 pKa = 5.96 RR264 pKa = 11.84 IKK266 pKa = 11.04 SLDD269 pKa = 3.48 NNNVTFTVKK278 pKa = 10.4 DD279 pKa = 3.68 YY280 pKa = 11.17 RR281 pKa = 11.84 HH282 pKa = 6.47 GGRR285 pKa = 11.84 KK286 pKa = 9.05 SLLRR290 pKa = 11.84 LTDD293 pKa = 3.21 AEE295 pKa = 4.29 FVRR298 pKa = 11.84 RR299 pKa = 11.84 FALHH303 pKa = 6.73 ILPKK307 pKa = 8.86 GFVRR311 pKa = 11.84 IRR313 pKa = 11.84 HH314 pKa = 5.2 YY315 pKa = 11.2 GILSSSRR322 pKa = 11.84 KK323 pKa = 9.3 KK324 pKa = 10.78 ALLPLLQQVLGGPVPNGRR342 pKa = 11.84 PPLKK346 pKa = 10.12 HH347 pKa = 6.24 RR348 pKa = 11.84 RR349 pKa = 11.84 CPYY352 pKa = 8.31 CQIGIMVTVAVFDD365 pKa = 3.83 SRR367 pKa = 11.84 GPPKK371 pKa = 10.42 AWSDD375 pKa = 3.52 RR376 pKa = 11.84 LKK378 pKa = 11.13 NSAPRR383 pKa = 11.84 RR384 pKa = 11.84 TPQKK388 pKa = 10.74 AA389 pKa = 2.8
Molecular weight: 44.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.75
IPC_protein 10.335
Toseland 10.628
ProMoST 10.262
Dawson 10.73
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 11.082
Grimsley 10.789
Solomon 10.804
Lehninger 10.774
Nozaki 10.628
DTASelect 10.409
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.657
Patrickios 10.76
IPC_peptide 10.804
IPC2_peptide 9.516
IPC2.peptide.svr19 8.524
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3553
0
3553
1230055
34
2809
346.2
38.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.643 ± 0.034
0.728 ± 0.012
5.5 ± 0.029
6.582 ± 0.037
5.077 ± 0.031
6.738 ± 0.043
1.905 ± 0.024
7.545 ± 0.035
7.606 ± 0.047
9.488 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.275 ± 0.02
5.786 ± 0.039
3.597 ± 0.024
3.434 ± 0.024
3.611 ± 0.024
6.437 ± 0.034
5.66 ± 0.044
6.195 ± 0.034
1.141 ± 0.018
4.055 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here