Arthrobacter phage MargaretKali

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345KN27|A0A345KN27_9CAUD Uncharacterized protein OS=Arthrobacter phage MargaretKali OX=2250414 GN=49 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 4.82EE3 pKa = 4.41FGEE6 pKa = 4.57TPSIYY11 pKa = 10.06VWPAGHH17 pKa = 6.69EE18 pKa = 4.08EE19 pKa = 4.68GEE21 pKa = 4.25PFPDD25 pKa = 4.16DD26 pKa = 3.16FWGRR30 pKa = 11.84VRR32 pKa = 11.84DD33 pKa = 3.7GLKK36 pKa = 10.56AVGIEE41 pKa = 4.33CEE43 pKa = 4.47TVV45 pKa = 2.72

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345KN33|A0A345KN33_9CAUD Uncharacterized protein OS=Arthrobacter phage MargaretKali OX=2250414 GN=55 PE=4 SV=1
MM1 pKa = 7.39CRR3 pKa = 11.84SATGVCLTRR12 pKa = 11.84FQCDD16 pKa = 2.92HH17 pKa = 7.0HH18 pKa = 8.01KK19 pKa = 11.09DD20 pKa = 3.05ADD22 pKa = 3.72KK23 pKa = 10.96RR24 pKa = 11.84DD25 pKa = 3.99EE26 pKa = 4.69ANHH29 pKa = 4.93QARR32 pKa = 11.84RR33 pKa = 11.84TYY35 pKa = 11.12NDD37 pKa = 3.17PTSRR41 pKa = 11.84QAVNNVLAAQRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84KK55 pKa = 9.18PRR57 pKa = 3.32

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12008

39

1143

166.8

18.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.726 ± 0.428

0.991 ± 0.193

5.888 ± 0.183

6.454 ± 0.42

3.156 ± 0.265

8.628 ± 0.527

2.182 ± 0.171

4.63 ± 0.233

4.755 ± 0.275

7.487 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.097

2.99 ± 0.165

5.546 ± 0.225

3.723 ± 0.2

6.079 ± 0.468

5.305 ± 0.209

6.862 ± 0.36

6.845 ± 0.273

2.099 ± 0.125

2.39 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski