Amino acid dipepetide frequency for Arthrobacter phage MargaretKali

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.323AlaAla: 17.323 ± 1.464
0.833AlaCys: 0.833 ± 0.312
6.08AlaAsp: 6.08 ± 0.859
8.079AlaGlu: 8.079 ± 1.15
3.998AlaPhe: 3.998 ± 1.039
10.077AlaGly: 10.077 ± 1.47
1.582AlaHis: 1.582 ± 0.298
4.581AlaIle: 4.581 ± 0.616
5.913AlaLys: 5.913 ± 0.845
10.077AlaLeu: 10.077 ± 1.145
3.581AlaMet: 3.581 ± 0.448
3.331AlaAsn: 3.331 ± 0.589
4.997AlaPro: 4.997 ± 0.696
5.164AlaGln: 5.164 ± 0.728
6.413AlaArg: 6.413 ± 0.908
5.913AlaSer: 5.913 ± 0.814
6.413AlaThr: 6.413 ± 0.637
8.328AlaVal: 8.328 ± 0.697
2.332AlaTrp: 2.332 ± 0.341
2.249AlaTyr: 2.249 ± 0.499
0.0AlaXaa: 0.0 ± 0.0
Cys
0.5CysAla: 0.5 ± 0.202
0.0CysCys: 0.0 ± 0.0
0.75CysAsp: 0.75 ± 0.267
0.666CysGlu: 0.666 ± 0.256
0.416CysPhe: 0.416 ± 0.189
1.083CysGly: 1.083 ± 0.34
0.25CysHis: 0.25 ± 0.151
0.167CysIle: 0.167 ± 0.106
0.75CysLys: 0.75 ± 0.258
0.5CysLeu: 0.5 ± 0.235
0.0CysMet: 0.0 ± 0.0
0.75CysAsn: 0.75 ± 0.258
0.75CysPro: 0.75 ± 0.317
0.333CysGln: 0.333 ± 0.145
0.833CysArg: 0.833 ± 0.304
0.583CysSer: 0.583 ± 0.221
0.666CysThr: 0.666 ± 0.276
0.5CysVal: 0.5 ± 0.267
0.333CysTrp: 0.333 ± 0.201
0.083CysTyr: 0.083 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
6.163AspAla: 6.163 ± 0.862
0.666AspCys: 0.666 ± 0.24
3.665AspAsp: 3.665 ± 0.613
4.497AspGlu: 4.497 ± 0.532
2.082AspPhe: 2.082 ± 0.403
6.163AspGly: 6.163 ± 0.607
2.082AspHis: 2.082 ± 0.384
3.082AspIle: 3.082 ± 0.473
2.332AspLys: 2.332 ± 0.4
4.914AspLeu: 4.914 ± 0.593
1.083AspMet: 1.083 ± 0.277
1.333AspAsn: 1.333 ± 0.314
3.331AspPro: 3.331 ± 0.494
2.332AspGln: 2.332 ± 0.391
2.332AspArg: 2.332 ± 0.514
2.332AspSer: 2.332 ± 0.598
3.165AspThr: 3.165 ± 0.537
3.914AspVal: 3.914 ± 0.464
1.582AspTrp: 1.582 ± 0.404
1.832AspTyr: 1.832 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
9.494GluAla: 9.494 ± 1.016
0.999GluCys: 0.999 ± 0.287
4.331GluAsp: 4.331 ± 0.747
3.165GluGlu: 3.165 ± 0.731
1.832GluPhe: 1.832 ± 0.385
4.331GluGly: 4.331 ± 0.593
1.166GluHis: 1.166 ± 0.287
3.165GluIle: 3.165 ± 0.429
1.999GluLys: 1.999 ± 0.476
5.08GluLeu: 5.08 ± 0.611
1.083GluMet: 1.083 ± 0.33
2.332GluAsn: 2.332 ± 0.466
3.998GluPro: 3.998 ± 0.643
2.499GluGln: 2.499 ± 0.544
4.831GluArg: 4.831 ± 0.82
3.498GluSer: 3.498 ± 0.572
3.914GluThr: 3.914 ± 0.595
3.581GluVal: 3.581 ± 0.504
1.499GluTrp: 1.499 ± 0.336
1.749GluTyr: 1.749 ± 0.408
0.0GluXaa: 0.0 ± 0.0
Phe
2.915PheAla: 2.915 ± 0.568
0.5PheCys: 0.5 ± 0.222
2.082PheAsp: 2.082 ± 0.415
1.582PheGlu: 1.582 ± 0.395
1.083PhePhe: 1.083 ± 0.242
3.165PheGly: 3.165 ± 0.633
0.916PheHis: 0.916 ± 0.251
2.249PheIle: 2.249 ± 0.531
1.416PheLys: 1.416 ± 0.258
1.666PheLeu: 1.666 ± 0.332
0.833PheMet: 0.833 ± 0.249
0.416PheAsn: 0.416 ± 0.155
0.999PhePro: 0.999 ± 0.28
1.333PheGln: 1.333 ± 0.366
1.999PheArg: 1.999 ± 0.553
2.415PheSer: 2.415 ± 0.433
2.249PheThr: 2.249 ± 0.526
2.499PheVal: 2.499 ± 0.451
0.833PheTrp: 0.833 ± 0.291
0.416PheTyr: 0.416 ± 0.156
0.0PheXaa: 0.0 ± 0.0
Gly
6.746GlyAla: 6.746 ± 1.262
0.416GlyCys: 0.416 ± 0.194
4.664GlyAsp: 4.664 ± 0.567
4.997GlyGlu: 4.997 ± 0.617
2.415GlyPhe: 2.415 ± 0.451
8.662GlyGly: 8.662 ± 1.383
2.415GlyHis: 2.415 ± 0.468
4.497GlyIle: 4.497 ± 0.771
4.414GlyLys: 4.414 ± 0.512
7.496GlyLeu: 7.496 ± 1.161
2.415GlyMet: 2.415 ± 0.371
2.332GlyAsn: 2.332 ± 0.423
4.581GlyPro: 4.581 ± 0.483
2.998GlyGln: 2.998 ± 0.549
4.747GlyArg: 4.747 ± 0.715
5.247GlySer: 5.247 ± 0.826
4.747GlyThr: 4.747 ± 0.591
6.413GlyVal: 6.413 ± 0.726
3.082GlyTrp: 3.082 ± 0.551
2.915GlyTyr: 2.915 ± 0.478
0.0GlyXaa: 0.0 ± 0.0
His
1.999HisAla: 1.999 ± 0.431
0.416HisCys: 0.416 ± 0.169
1.249HisAsp: 1.249 ± 0.358
1.249HisGlu: 1.249 ± 0.395
0.416HisPhe: 0.416 ± 0.184
1.832HisGly: 1.832 ± 0.332
0.916HisHis: 0.916 ± 0.319
0.999HisIle: 0.999 ± 0.341
0.75HisLys: 0.75 ± 0.293
2.332HisLeu: 2.332 ± 0.433
0.416HisMet: 0.416 ± 0.174
0.833HisAsn: 0.833 ± 0.216
1.666HisPro: 1.666 ± 0.368
1.416HisGln: 1.416 ± 0.33
1.333HisArg: 1.333 ± 0.32
0.75HisSer: 0.75 ± 0.248
0.999HisThr: 0.999 ± 0.295
1.499HisVal: 1.499 ± 0.298
0.416HisTrp: 0.416 ± 0.178
0.333HisTyr: 0.333 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.08IleAla: 5.08 ± 0.624
0.416IleCys: 0.416 ± 0.218
2.249IleAsp: 2.249 ± 0.392
3.665IleGlu: 3.665 ± 0.713
1.749IlePhe: 1.749 ± 0.442
5.663IleGly: 5.663 ± 0.812
1.333IleHis: 1.333 ± 0.356
1.749IleIle: 1.749 ± 0.415
1.832IleLys: 1.832 ± 0.363
3.165IleLeu: 3.165 ± 0.597
0.833IleMet: 0.833 ± 0.252
1.083IleAsn: 1.083 ± 0.289
2.332IlePro: 2.332 ± 0.374
2.082IleGln: 2.082 ± 0.389
2.082IleArg: 2.082 ± 0.458
2.582IleSer: 2.582 ± 0.415
3.831IleThr: 3.831 ± 0.663
3.248IleVal: 3.248 ± 0.441
0.416IleTrp: 0.416 ± 0.22
0.916IleTyr: 0.916 ± 0.272
0.0IleXaa: 0.0 ± 0.0
Lys
7.329LysAla: 7.329 ± 0.788
0.75LysCys: 0.75 ± 0.326
2.665LysAsp: 2.665 ± 0.635
2.665LysGlu: 2.665 ± 0.531
1.083LysPhe: 1.083 ± 0.302
2.832LysGly: 2.832 ± 0.558
1.249LysHis: 1.249 ± 0.292
1.416LysIle: 1.416 ± 0.334
2.748LysLys: 2.748 ± 0.485
3.248LysLeu: 3.248 ± 0.5
1.333LysMet: 1.333 ± 0.281
1.249LysAsn: 1.249 ± 0.308
2.499LysPro: 2.499 ± 0.536
1.999LysGln: 1.999 ± 0.529
3.415LysArg: 3.415 ± 0.507
2.249LysSer: 2.249 ± 0.432
3.914LysThr: 3.914 ± 0.574
2.832LysVal: 2.832 ± 0.431
0.999LysTrp: 0.999 ± 0.32
1.083LysTyr: 1.083 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
9.828LeuAla: 9.828 ± 0.811
0.5LeuCys: 0.5 ± 0.206
4.164LeuAsp: 4.164 ± 0.624
5.497LeuGlu: 5.497 ± 0.641
2.249LeuPhe: 2.249 ± 0.407
5.497LeuGly: 5.497 ± 0.769
1.749LeuHis: 1.749 ± 0.358
3.498LeuIle: 3.498 ± 0.459
2.915LeuLys: 2.915 ± 0.412
4.914LeuLeu: 4.914 ± 0.758
1.666LeuMet: 1.666 ± 0.368
2.415LeuAsn: 2.415 ± 0.65
4.581LeuPro: 4.581 ± 0.64
2.915LeuGln: 2.915 ± 0.535
3.914LeuArg: 3.914 ± 0.682
3.665LeuSer: 3.665 ± 0.497
6.496LeuThr: 6.496 ± 0.817
5.247LeuVal: 5.247 ± 0.591
1.416LeuTrp: 1.416 ± 0.353
1.749LeuTyr: 1.749 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
2.832MetAla: 2.832 ± 0.455
0.167MetCys: 0.167 ± 0.121
1.333MetAsp: 1.333 ± 0.269
0.666MetGlu: 0.666 ± 0.281
0.75MetPhe: 0.75 ± 0.209
1.999MetGly: 1.999 ± 0.264
0.333MetHis: 0.333 ± 0.145
0.75MetIle: 0.75 ± 0.261
1.166MetLys: 1.166 ± 0.277
1.666MetLeu: 1.666 ± 0.405
0.25MetMet: 0.25 ± 0.132
0.583MetAsn: 0.583 ± 0.189
1.416MetPro: 1.416 ± 0.427
0.583MetGln: 0.583 ± 0.205
1.832MetArg: 1.832 ± 0.427
2.332MetSer: 2.332 ± 0.341
1.999MetThr: 1.999 ± 0.428
1.582MetVal: 1.582 ± 0.392
0.083MetTrp: 0.083 ± 0.077
0.333MetTyr: 0.333 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
2.499AsnAla: 2.499 ± 0.447
0.25AsnCys: 0.25 ± 0.137
1.832AsnAsp: 1.832 ± 0.318
1.832AsnGlu: 1.832 ± 0.352
0.75AsnPhe: 0.75 ± 0.239
3.914AsnGly: 3.914 ± 0.576
0.75AsnHis: 0.75 ± 0.299
1.083AsnIle: 1.083 ± 0.297
0.999AsnLys: 0.999 ± 0.268
2.582AsnLeu: 2.582 ± 0.502
0.75AsnMet: 0.75 ± 0.209
1.333AsnAsn: 1.333 ± 0.309
1.999AsnPro: 1.999 ± 0.431
1.499AsnGln: 1.499 ± 0.324
2.082AsnArg: 2.082 ± 0.363
1.166AsnSer: 1.166 ± 0.288
1.666AsnThr: 1.666 ± 0.474
1.916AsnVal: 1.916 ± 0.419
0.5AsnTrp: 0.5 ± 0.167
0.5AsnTyr: 0.5 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
6.413ProAla: 6.413 ± 0.743
0.75ProCys: 0.75 ± 0.229
3.748ProAsp: 3.748 ± 0.684
4.331ProGlu: 4.331 ± 0.532
2.082ProPhe: 2.082 ± 0.41
5.08ProGly: 5.08 ± 0.74
0.833ProHis: 0.833 ± 0.23
2.499ProIle: 2.499 ± 0.476
2.665ProLys: 2.665 ± 0.633
3.748ProLeu: 3.748 ± 0.685
1.333ProMet: 1.333 ± 0.329
1.916ProAsn: 1.916 ± 0.353
2.582ProPro: 2.582 ± 0.477
1.416ProGln: 1.416 ± 0.292
2.165ProArg: 2.165 ± 0.312
3.082ProSer: 3.082 ± 0.528
4.248ProThr: 4.248 ± 0.676
3.914ProVal: 3.914 ± 0.539
1.166ProTrp: 1.166 ± 0.277
1.499ProTyr: 1.499 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
4.664GlnAla: 4.664 ± 0.68
0.5GlnCys: 0.5 ± 0.215
1.499GlnAsp: 1.499 ± 0.378
2.499GlnGlu: 2.499 ± 0.457
1.166GlnPhe: 1.166 ± 0.297
1.916GlnGly: 1.916 ± 0.393
0.833GlnHis: 0.833 ± 0.324
1.832GlnIle: 1.832 ± 0.431
1.832GlnLys: 1.832 ± 0.398
3.665GlnLeu: 3.665 ± 0.52
0.833GlnMet: 0.833 ± 0.311
0.333GlnAsn: 0.333 ± 0.183
2.832GlnPro: 2.832 ± 0.407
1.333GlnGln: 1.333 ± 0.341
3.165GlnArg: 3.165 ± 0.512
1.832GlnSer: 1.832 ± 0.349
2.249GlnThr: 2.249 ± 0.531
2.415GlnVal: 2.415 ± 0.52
0.916GlnTrp: 0.916 ± 0.217
0.916GlnTyr: 0.916 ± 0.307
0.0GlnXaa: 0.0 ± 0.0
Arg
6.996ArgAla: 6.996 ± 0.893
0.916ArgCys: 0.916 ± 0.338
4.414ArgAsp: 4.414 ± 0.503
4.414ArgGlu: 4.414 ± 0.732
1.749ArgPhe: 1.749 ± 0.319
3.331ArgGly: 3.331 ± 0.442
1.166ArgHis: 1.166 ± 0.336
3.748ArgIle: 3.748 ± 0.719
3.998ArgLys: 3.998 ± 0.557
3.331ArgLeu: 3.331 ± 0.621
1.666ArgMet: 1.666 ± 0.393
1.916ArgAsn: 1.916 ± 0.429
3.748ArgPro: 3.748 ± 0.659
2.082ArgGln: 2.082 ± 0.432
3.831ArgArg: 3.831 ± 0.808
2.665ArgSer: 2.665 ± 0.423
4.414ArgThr: 4.414 ± 0.656
3.914ArgVal: 3.914 ± 0.588
1.333ArgTrp: 1.333 ± 0.357
1.166ArgTyr: 1.166 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
7.079SerAla: 7.079 ± 0.862
0.5SerCys: 0.5 ± 0.219
2.665SerAsp: 2.665 ± 0.383
2.748SerGlu: 2.748 ± 0.399
2.249SerPhe: 2.249 ± 0.519
5.08SerGly: 5.08 ± 0.599
1.166SerHis: 1.166 ± 0.305
2.249SerIle: 2.249 ± 0.39
2.748SerLys: 2.748 ± 0.387
3.498SerLeu: 3.498 ± 0.532
0.999SerMet: 0.999 ± 0.278
1.499SerAsn: 1.499 ± 0.348
2.582SerPro: 2.582 ± 0.512
1.582SerGln: 1.582 ± 0.334
4.497SerArg: 4.497 ± 0.71
2.748SerSer: 2.748 ± 0.591
3.415SerThr: 3.415 ± 0.563
3.331SerVal: 3.331 ± 0.505
0.833SerTrp: 0.833 ± 0.343
1.582SerTyr: 1.582 ± 0.383
0.0SerXaa: 0.0 ± 0.0
Thr
7.079ThrAla: 7.079 ± 0.831
0.416ThrCys: 0.416 ± 0.207
4.248ThrAsp: 4.248 ± 0.752
4.664ThrGlu: 4.664 ± 0.615
2.249ThrPhe: 2.249 ± 0.477
6.246ThrGly: 6.246 ± 1.043
1.083ThrHis: 1.083 ± 0.335
3.748ThrIle: 3.748 ± 0.588
3.082ThrLys: 3.082 ± 0.569
4.914ThrLeu: 4.914 ± 0.634
1.582ThrMet: 1.582 ± 0.366
2.499ThrAsn: 2.499 ± 0.55
4.248ThrPro: 4.248 ± 0.658
1.999ThrGln: 1.999 ± 0.405
2.998ThrArg: 2.998 ± 0.481
3.581ThrSer: 3.581 ± 0.459
5.164ThrThr: 5.164 ± 0.791
5.414ThrVal: 5.414 ± 0.729
1.166ThrTrp: 1.166 ± 0.308
2.249ThrTyr: 2.249 ± 0.426
0.0ThrXaa: 0.0 ± 0.0
Val
7.329ValAla: 7.329 ± 0.924
0.333ValCys: 0.333 ± 0.17
3.914ValAsp: 3.914 ± 0.559
4.414ValGlu: 4.414 ± 0.556
2.165ValPhe: 2.165 ± 0.476
5.33ValGly: 5.33 ± 0.871
0.999ValHis: 0.999 ± 0.247
3.248ValIle: 3.248 ± 0.428
4.164ValLys: 4.164 ± 0.662
4.414ValLeu: 4.414 ± 0.479
1.249ValMet: 1.249 ± 0.268
2.499ValAsn: 2.499 ± 0.443
3.665ValPro: 3.665 ± 0.662
1.582ValGln: 1.582 ± 0.398
5.497ValArg: 5.497 ± 0.571
3.665ValSer: 3.665 ± 0.452
5.997ValThr: 5.997 ± 0.798
4.997ValVal: 4.997 ± 0.762
1.499ValTrp: 1.499 ± 0.489
1.499ValTyr: 1.499 ± 0.362
0.0ValXaa: 0.0 ± 0.0
Trp
1.916TrpAla: 1.916 ± 0.535
0.167TrpCys: 0.167 ± 0.119
1.916TrpAsp: 1.916 ± 0.441
1.249TrpGlu: 1.249 ± 0.277
0.583TrpPhe: 0.583 ± 0.191
1.416TrpGly: 1.416 ± 0.335
0.583TrpHis: 0.583 ± 0.22
0.583TrpIle: 0.583 ± 0.199
0.833TrpLys: 0.833 ± 0.265
1.749TrpLeu: 1.749 ± 0.443
0.25TrpMet: 0.25 ± 0.139
0.916TrpAsn: 0.916 ± 0.252
1.666TrpPro: 1.666 ± 0.363
0.999TrpGln: 0.999 ± 0.247
1.582TrpArg: 1.582 ± 0.359
1.166TrpSer: 1.166 ± 0.315
1.166TrpThr: 1.166 ± 0.381
1.333TrpVal: 1.333 ± 0.253
0.416TrpTrp: 0.416 ± 0.163
0.5TrpTyr: 0.5 ± 0.187
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.082TyrAla: 3.082 ± 0.575
0.416TyrCys: 0.416 ± 0.227
1.416TyrAsp: 1.416 ± 0.297
1.666TyrGlu: 1.666 ± 0.312
0.5TyrPhe: 0.5 ± 0.204
2.665TyrGly: 2.665 ± 0.427
0.416TyrHis: 0.416 ± 0.204
1.166TyrIle: 1.166 ± 0.305
0.999TyrLys: 0.999 ± 0.293
1.916TyrLeu: 1.916 ± 0.375
0.416TyrMet: 0.416 ± 0.18
0.333TyrAsn: 0.333 ± 0.179
0.999TyrPro: 0.999 ± 0.266
1.083TyrGln: 1.083 ± 0.331
1.249TyrArg: 1.249 ± 0.361
1.582TyrSer: 1.582 ± 0.382
1.916TyrThr: 1.916 ± 0.33
1.582TyrVal: 1.582 ± 0.314
0.167TyrTrp: 0.167 ± 0.11
0.333TyrTyr: 0.333 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (12008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski