Streptococcus phage Javan213

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A326|A0A4D6A326_9CAUD Lysozyme OS=Streptococcus phage Javan213 OX=2548049 GN=Javan213_0005 PE=3 SV=1
MM1 pKa = 7.87AYY3 pKa = 10.08NWQQFKK9 pKa = 11.2NGLFGFSYY17 pKa = 10.42DD18 pKa = 3.82YY19 pKa = 11.2EE20 pKa = 5.08DD21 pKa = 4.93NGLLDD26 pKa = 3.75SLRR29 pKa = 11.84NDD31 pKa = 3.06IADD34 pKa = 3.44NKK36 pKa = 9.82IGNKK40 pKa = 9.26IVVIRR45 pKa = 11.84YY46 pKa = 9.05RR47 pKa = 11.84RR48 pKa = 11.84DD49 pKa = 3.18DD50 pKa = 3.16TDD52 pKa = 3.12YY53 pKa = 11.51RR54 pKa = 11.84PIRR57 pKa = 11.84DD58 pKa = 3.58YY59 pKa = 10.99LYY61 pKa = 9.93EE62 pKa = 5.4GEE64 pKa = 4.8AVSEE68 pKa = 4.05DD69 pKa = 3.43EE70 pKa = 4.97EE71 pKa = 5.09YY72 pKa = 11.26SFEE75 pKa = 4.06NTADD79 pKa = 3.47VLEE82 pKa = 4.57EE83 pKa = 4.11MEE85 pKa = 4.18AMTRR89 pKa = 11.84IFF91 pKa = 4.21

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A352|A0A4D6A352_9CAUD Uncharacterized protein OS=Streptococcus phage Javan213 OX=2548049 GN=Javan213_0037 PE=4 SV=1
MM1 pKa = 7.28LASAIGLYY9 pKa = 10.22GVSSLTEE16 pKa = 4.04AKK18 pKa = 10.47RR19 pKa = 11.84MTIEE23 pKa = 3.76EE24 pKa = 4.17FNVRR28 pKa = 11.84KK29 pKa = 9.78RR30 pKa = 11.84GYY32 pKa = 10.75LMQRR36 pKa = 11.84LDD38 pKa = 3.91RR39 pKa = 11.84EE40 pKa = 4.22RR41 pKa = 11.84EE42 pKa = 4.12LYY44 pKa = 9.53LQAYY48 pKa = 8.89LNRR51 pKa = 11.84LVKK54 pKa = 10.21ATDD57 pKa = 3.34KK58 pKa = 11.17SGKK61 pKa = 9.2QYY63 pKa = 11.24VYY65 pKa = 11.17GKK67 pKa = 10.85FEE69 pKa = 4.51DD70 pKa = 4.97FYY72 pKa = 11.98NEE74 pKa = 3.67AKK76 pKa = 10.0QRR78 pKa = 11.84NAVLGNGHH86 pKa = 6.32GNAVNSDD93 pKa = 3.41LVAIARR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84QKK103 pKa = 10.4FLKK106 pKa = 10.59EE107 pKa = 4.17GGNKK111 pKa = 9.45

Molecular weight: 12.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11531

43

1137

209.7

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.152 ± 0.528

0.572 ± 0.094

6.409 ± 0.338

7.241 ± 0.64

3.894 ± 0.233

6.287 ± 0.427

1.188 ± 0.12

6.175 ± 0.349

7.909 ± 0.492

8.057 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.199

5.888 ± 0.258

2.506 ± 0.14

4.544 ± 0.315

4.137 ± 0.353

6.339 ± 0.457

6.591 ± 0.545

6.374 ± 0.304

1.327 ± 0.174

4.076 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski