Jannaschia aquimarina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Jannaschia

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4049 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1EBF1|A0A0D1EBF1_9RHOB EamA-like transporter family protein OS=Jannaschia aquimarina OX=935700 GN=jaqu_40300 PE=4 SV=1
MM1 pKa = 7.79DD2 pKa = 5.44RR3 pKa = 11.84APVTGRR9 pKa = 11.84NITKK13 pKa = 9.43TIQLASASALALVGATFGTAAAAEE37 pKa = 4.28DD38 pKa = 4.73LTLCWAAWDD47 pKa = 3.79PANALVEE54 pKa = 3.93LSKK57 pKa = 11.18DD58 pKa = 3.39FEE60 pKa = 4.44EE61 pKa = 5.12QSGHH65 pKa = 4.04TMNFEE70 pKa = 4.18FVPWPNFADD79 pKa = 3.92RR80 pKa = 11.84MLNEE84 pKa = 4.6LNSGGQLCDD93 pKa = 4.12LMIGDD98 pKa = 4.62SQWIGGGAEE107 pKa = 3.67NGHH110 pKa = 5.09YY111 pKa = 10.7VKK113 pKa = 11.07LNDD116 pKa = 3.73FFEE119 pKa = 5.99ASDD122 pKa = 3.28ISMDD126 pKa = 3.96DD127 pKa = 3.39FADD130 pKa = 3.77AAVYY134 pKa = 9.81AYY136 pKa = 7.65STWPKK141 pKa = 9.25GTEE144 pKa = 3.61NYY146 pKa = 9.38YY147 pKa = 11.01ALPAMGDD154 pKa = 3.45ANGWFYY160 pKa = 11.3RR161 pKa = 11.84KK162 pKa = 9.89DD163 pKa = 3.1WFTDD167 pKa = 3.65PEE169 pKa = 3.79IMAAFEE175 pKa = 4.49AEE177 pKa = 3.94HH178 pKa = 6.27GRR180 pKa = 11.84PLGEE184 pKa = 4.04PQTQRR189 pKa = 11.84EE190 pKa = 4.3LMEE193 pKa = 3.96IAKK196 pKa = 9.06FFQGRR201 pKa = 11.84EE202 pKa = 3.34IDD204 pKa = 3.55GQEE207 pKa = 3.91RR208 pKa = 11.84YY209 pKa = 9.79GVSIFTEE216 pKa = 4.45RR217 pKa = 11.84GSEE220 pKa = 4.3GITMGVTGALYY231 pKa = 10.09AWGLEE236 pKa = 4.18YY237 pKa = 10.82EE238 pKa = 4.6NEE240 pKa = 4.13PGSYY244 pKa = 11.42DD245 pKa = 3.08MVGAMNSPEE254 pKa = 3.93AVEE257 pKa = 4.49ALEE260 pKa = 5.59FYY262 pKa = 10.67TEE264 pKa = 4.99LYY266 pKa = 9.22EE267 pKa = 4.6CCTPPGYY274 pKa = 9.33TNSYY278 pKa = 8.86MGEE281 pKa = 3.96SLDD284 pKa = 3.81AFKK287 pKa = 10.9SGQVAMAMNWFAFFPGLYY305 pKa = 9.69ADD307 pKa = 4.8PDD309 pKa = 3.51IGGDD313 pKa = 3.51KK314 pKa = 10.18IDD316 pKa = 4.49FFVNPPQNQEE326 pKa = 3.33ASTLGGQGISVVSYY340 pKa = 10.41PDD342 pKa = 3.31KK343 pKa = 10.94QDD345 pKa = 3.19AALEE349 pKa = 4.19YY350 pKa = 10.08IAWFAQPEE358 pKa = 4.37VQKK361 pKa = 10.64QWWALGGYY369 pKa = 8.97AVHH372 pKa = 7.39DD373 pKa = 4.21AVLEE377 pKa = 4.36DD378 pKa = 3.72PAFEE382 pKa = 4.1EE383 pKa = 4.49SQPFAGDD390 pKa = 3.59FLQAMNAVQDD400 pKa = 4.34FWQEE404 pKa = 3.47PTYY407 pKa = 11.4AEE409 pKa = 4.76LLLAMQEE416 pKa = 4.15RR417 pKa = 11.84VHH419 pKa = 7.17NYY421 pKa = 8.34VVADD425 pKa = 3.36QGTAQEE431 pKa = 4.86ALDD434 pKa = 4.46ALIEE438 pKa = 4.4DD439 pKa = 3.73WTEE442 pKa = 3.71VFEE445 pKa = 6.98DD446 pKa = 4.36DD447 pKa = 5.22GKK449 pKa = 11.47LL450 pKa = 3.4

Molecular weight: 49.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1EL05|A0A0D1EL05_9RHOB Uncharacterized protein OS=Jannaschia aquimarina OX=935700 GN=jaqu_05570 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4049

0

4049

1221941

29

2795

301.8

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.113 ± 0.062

0.844 ± 0.012

6.527 ± 0.037

6.065 ± 0.04

3.478 ± 0.022

9.123 ± 0.049

1.988 ± 0.021

4.721 ± 0.025

2.269 ± 0.033

10.155 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.022

2.104 ± 0.024

5.455 ± 0.034

2.724 ± 0.022

7.68 ± 0.045

4.78 ± 0.024

5.493 ± 0.036

7.406 ± 0.036

1.516 ± 0.016

1.967 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski