Flavobacterium haoranii
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2604 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6IW77|A0A1M6IW77_9FLAO L-threonine dehydratase OS=Flavobacterium haoranii OX=683124 GN=ilvA PE=3 SV=1
MM1 pKa = 7.96 RR2 pKa = 11.84 KK3 pKa = 10.09 NNMNKK8 pKa = 9.44 ILMSSLLALALGFVSCDD25 pKa = 3.54 NQDD28 pKa = 3.58 DD29 pKa = 4.04 ATGDD33 pKa = 3.67 SVFEE37 pKa = 4.06 VTEE40 pKa = 4.23 GVVASVTTDD49 pKa = 3.78 FDD51 pKa = 3.67 NSATITLNEE60 pKa = 3.91 YY61 pKa = 10.75 DD62 pKa = 3.88 EE63 pKa = 5.27 EE64 pKa = 4.29 EE65 pKa = 4.45 FPFTITLNKK74 pKa = 8.92 PSSIATRR81 pKa = 11.84 FRR83 pKa = 11.84 IVQIGGDD90 pKa = 3.51 AVLGEE95 pKa = 4.9 DD96 pKa = 4.43 YY97 pKa = 10.9 EE98 pKa = 4.31 ILNLSDD104 pKa = 3.64 EE105 pKa = 4.84 SIEE108 pKa = 4.14 FLDD111 pKa = 3.64 IPARR115 pKa = 11.84 EE116 pKa = 4.15 TTVSGKK122 pKa = 10.08 IKK124 pKa = 10.16 ILSDD128 pKa = 3.34 IYY130 pKa = 11.63 VEE132 pKa = 4.17 DD133 pKa = 4.03 TKK135 pKa = 11.01 TLSIQIGNQSLANATMQYY153 pKa = 10.34 EE154 pKa = 4.59 VVNFAIEE161 pKa = 5.01 DD162 pKa = 3.77 YY163 pKa = 10.6 DD164 pKa = 4.11 SSEE167 pKa = 4.53 MIFEE171 pKa = 4.65 LNYY174 pKa = 10.77 GKK176 pKa = 10.28 EE177 pKa = 4.11 FYY179 pKa = 10.49 YY180 pKa = 10.21 QGEE183 pKa = 4.38 MTTTCDD189 pKa = 3.29 FEE191 pKa = 6.11 YY192 pKa = 11.32 DD193 pKa = 2.9 MDD195 pKa = 4.32 YY196 pKa = 11.48 LVFDD200 pKa = 4.88 SNFDD204 pKa = 3.35 DD205 pKa = 3.48 TGIYY209 pKa = 10.54 DD210 pKa = 4.45 GATGACPTEE219 pKa = 4.21 SLSVSVDD226 pKa = 3.37 PSHH229 pKa = 7.6 PNYY232 pKa = 10.74 LPDD235 pKa = 3.07 GTYY238 pKa = 10.74 YY239 pKa = 10.28 IFYY242 pKa = 10.5 SVWDD246 pKa = 4.27 DD247 pKa = 3.66 GGLSGLYY254 pKa = 9.6 HH255 pKa = 7.51 DD256 pKa = 5.34 VFTIPTSVEE265 pKa = 4.36 FYY267 pKa = 10.86 RR268 pKa = 11.84 PGLLNPLNFDD278 pKa = 3.4 QEE280 pKa = 4.26 SAYY283 pKa = 9.99 IPDD286 pKa = 3.53 STYY289 pKa = 10.95 GSGAEE294 pKa = 4.49 DD295 pKa = 3.64 YY296 pKa = 11.41 VLTIEE301 pKa = 4.57 INSGVISVLDD311 pKa = 3.8 SNNSVLGSARR321 pKa = 11.84 FAVNHH326 pKa = 6.51 DD327 pKa = 3.94 KK328 pKa = 10.98 IKK330 pKa = 10.89 AAFANARR337 pKa = 11.84 AKK339 pKa = 10.37 SLKK342 pKa = 10.14 KK343 pKa = 10.57
Molecular weight: 37.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 0.884
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.895
Protein with the highest isoelectric point:
>tr|A0A1M6IW98|A0A1M6IW98_9FLAO Ketol-acid reductoisomerase OS=Flavobacterium haoranii OX=683124 GN=SAMN05444337_1891 PE=3 SV=1
MM1 pKa = 6.53 VTNLLHH7 pKa = 6.76 FFEE10 pKa = 4.38 KK11 pKa = 10.48 HH12 pKa = 5.13 GFFVAQRR19 pKa = 11.84 LAEE22 pKa = 4.05 RR23 pKa = 11.84 LGIRR27 pKa = 11.84 ATNVRR32 pKa = 11.84 LFFIYY37 pKa = 10.25 ISFITIGLGFGFYY50 pKa = 9.4 LTLAFLLRR58 pKa = 11.84 LKK60 pKa = 11.2 DD61 pKa = 3.61 MIHH64 pKa = 6.41 TKK66 pKa = 10.26 RR67 pKa = 11.84 SSVFDD72 pKa = 3.68 LL73 pKa = 4.35
Molecular weight: 8.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.794
IPC_protein 10.628
Toseland 10.687
ProMoST 10.394
Dawson 10.789
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.008
Grimsley 10.847
Solomon 10.906
Lehninger 10.877
Nozaki 10.643
DTASelect 10.511
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.833
IPC_peptide 10.921
IPC2_peptide 9.326
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2604
0
2604
857636
31
2560
329.4
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.045 ± 0.049
0.771 ± 0.018
5.25 ± 0.039
6.901 ± 0.049
5.562 ± 0.046
6.125 ± 0.045
1.675 ± 0.021
8.135 ± 0.053
8.163 ± 0.066
9.12 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.028
6.719 ± 0.061
3.212 ± 0.028
3.476 ± 0.025
3.04 ± 0.026
6.386 ± 0.041
5.776 ± 0.06
6.371 ± 0.041
0.97 ± 0.017
4.159 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here