SAR202 cluster bacterium AD-802-F09_MRT_200m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 5.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YNQ9|A0A5N8YNQ9_9CHLR DUF5681 domain-containing protein OS=SAR202 cluster bacterium AD-802-F09_MRT_200m OX=2587839 GN=FIM07_02610 PE=4 SV=1
MM1 pKa = 7.35LPDD4 pKa = 3.24ARR6 pKa = 11.84SRR8 pKa = 11.84LTHH11 pKa = 6.37CFSVVFPEE19 pKa = 5.32LEE21 pKa = 3.97EE22 pKa = 4.03QEE24 pKa = 4.5IPVSSIASVGAWDD37 pKa = 3.94SLASINLYY45 pKa = 10.66SLVEE49 pKa = 3.99EE50 pKa = 4.32EE51 pKa = 5.23FGVEE55 pKa = 3.9INMDD59 pKa = 3.85DD60 pKa = 4.32LEE62 pKa = 4.23NLISFEE68 pKa = 4.99LILGYY73 pKa = 10.03IEE75 pKa = 5.01GGNDD79 pKa = 2.99ASS81 pKa = 3.87

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YP99|A0A5N8YP99_9CHLR Xanthine dehydrogenase family protein molybdopterin-binding subunit OS=SAR202 cluster bacterium AD-802-F09_MRT_200m OX=2587839 GN=FIM07_03505 PE=4 SV=1
MM1 pKa = 6.78GAEE4 pKa = 4.18YY5 pKa = 10.29RR6 pKa = 11.84RR7 pKa = 11.84PGYY10 pKa = 8.69STSSAADD17 pKa = 3.19RR18 pKa = 11.84RR19 pKa = 11.84LRR21 pKa = 11.84RR22 pKa = 11.84NTLTWFKK29 pKa = 10.48DD30 pKa = 2.98ATIIYY35 pKa = 10.43SLGNLLARR43 pKa = 11.84FCFRR47 pKa = 11.84TFGRR51 pKa = 11.84LHH53 pKa = 5.87VSGAEE58 pKa = 4.06GVPKK62 pKa = 10.54FGPLIVVSNHH72 pKa = 6.08LSLNDD77 pKa = 3.71PPLLVATIPRR87 pKa = 11.84PLYY90 pKa = 10.5FIGKK94 pKa = 9.28KK95 pKa = 9.07EE96 pKa = 3.95LFGNPITRR104 pKa = 11.84LGMRR108 pKa = 11.84LFHH111 pKa = 6.3VSSFNRR117 pKa = 11.84SAAGIDD123 pKa = 3.44AVRR126 pKa = 11.84VLMQNLEE133 pKa = 3.78RR134 pKa = 11.84DD135 pKa = 3.46RR136 pKa = 11.84AVVIFPEE143 pKa = 5.18GTRR146 pKa = 11.84SPDD149 pKa = 3.3GALHH153 pKa = 6.76RR154 pKa = 11.84GMLGVVYY161 pKa = 10.26LALKK165 pKa = 9.53SQAAILPVGVTGTEE179 pKa = 4.17KK180 pKa = 10.64FPLWRR185 pKa = 11.84IPFPFRR191 pKa = 11.84KK192 pKa = 8.47MKK194 pKa = 10.79ASIGQPFTLPVIEE207 pKa = 4.39GTPSKK212 pKa = 10.93EE213 pKa = 4.13VMSSLTDD220 pKa = 3.28MVMSRR225 pKa = 11.84IADD228 pKa = 3.63QLPQEE233 pKa = 4.23YY234 pKa = 9.38RR235 pKa = 11.84GVYY238 pKa = 9.67ARR240 pKa = 11.84EE241 pKa = 3.77IKK243 pKa = 10.0QRR245 pKa = 11.84PAEE248 pKa = 4.02QAVV251 pKa = 3.17

Molecular weight: 27.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1047

0

1047

312120

36

1854

298.1

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.067 ± 0.084

0.962 ± 0.024

5.932 ± 0.064

6.333 ± 0.066

3.888 ± 0.051

8.712 ± 0.071

2.114 ± 0.03

5.833 ± 0.056

3.386 ± 0.044

9.479 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.049

3.36 ± 0.041

5.041 ± 0.05

3.378 ± 0.04

5.647 ± 0.064

6.543 ± 0.052

5.487 ± 0.048

7.771 ± 0.072

1.495 ± 0.029

2.811 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski