Vitis riparia x Vitis cinerea

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; rosids incertae sedis; Vitales; Vitaceae; Viteae; Vitis

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A654IBI0|A0A654IBI0_9ROSI 50S ribosomal protein L33 chloroplastic OS=Vitis riparia x Vitis cinerea OX=2018007 GN=rpl33 PE=3 SV=1
MM1 pKa = 6.86EE2 pKa = 4.58TATLVAISISGLLVSLTGYY21 pKa = 11.01ALYY24 pKa = 9.16TAFGQPSQQLRR35 pKa = 11.84DD36 pKa = 3.65PFEE39 pKa = 4.0EE40 pKa = 5.39HH41 pKa = 7.25GDD43 pKa = 3.49

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A654IBM9|A0A654IBM9_9ROSI Rps19 protein OS=Vitis riparia x Vitis cinerea OX=2018007 GN=rps19 PE=3 SV=1
MM1 pKa = 6.88VQLHH5 pKa = 6.19NSFFFITSMFVPRR18 pKa = 11.84GTASPALWKK27 pKa = 9.93WFVSRR32 pKa = 11.84EE33 pKa = 4.05IPTGAPSSNGTIIPIPIPSFPLLVYY58 pKa = 9.56LHH60 pKa = 6.06SRR62 pKa = 11.84KK63 pKa = 9.61FIRR66 pKa = 11.84FIDD69 pKa = 3.8RR70 pKa = 11.84AKK72 pKa = 10.84SGVLVRR78 pKa = 11.84ASHH81 pKa = 6.95PIILLSDD88 pKa = 4.14IIRR91 pKa = 11.84ISSSEE96 pKa = 4.06TIARR100 pKa = 11.84RR101 pKa = 11.84ASFRR105 pKa = 11.84FVPVLHH111 pKa = 6.6FLLLEE116 pKa = 4.31SKK118 pKa = 11.15GDD120 pKa = 3.44ISYY123 pKa = 11.02LEE125 pKa = 4.3SFCGVLRR132 pKa = 11.84LLFFRR137 pKa = 11.84TLLSLPRR144 pKa = 11.84DD145 pKa = 3.24RR146 pKa = 11.84SAKK149 pKa = 9.65RR150 pKa = 11.84EE151 pKa = 3.56RR152 pKa = 11.84ARR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84KK157 pKa = 9.5GQALRR162 pKa = 11.84PNGNEE167 pKa = 3.39QRR169 pKa = 11.84RR170 pKa = 11.84NDD172 pKa = 3.46KK173 pKa = 9.49MRR175 pKa = 11.84CPGHH179 pKa = 5.89PHH181 pKa = 6.52LEE183 pKa = 3.89RR184 pKa = 11.84RR185 pKa = 11.84VEE187 pKa = 4.14GFGPVAFPVPPSSGGACVGGVPPEE211 pKa = 4.18IGLEE215 pKa = 3.92ALALPTSRR223 pKa = 11.84QLRR226 pKa = 11.84AVGDD230 pKa = 3.89DD231 pKa = 4.18YY232 pKa = 11.59YY233 pKa = 11.63QKK235 pKa = 11.53APMKK239 pKa = 10.14MNISHH244 pKa = 6.86GGVCICMLGVLLSKK258 pKa = 9.33TKK260 pKa = 10.38KK261 pKa = 9.48IQFTQRR267 pKa = 11.84LPLGSEE273 pKa = 3.44LHH275 pKa = 5.92MGKK278 pKa = 9.29EE279 pKa = 4.39RR280 pKa = 11.84CCLRR284 pKa = 11.84GIDD287 pKa = 4.28HH288 pKa = 6.82LHH290 pKa = 6.57GPTSHH295 pKa = 6.86SICGNFMIYY304 pKa = 9.85KK305 pKa = 10.33RR306 pKa = 11.84SLTTDD311 pKa = 2.68RR312 pKa = 11.84LMFEE316 pKa = 4.38HH317 pKa = 7.53DD318 pKa = 3.13EE319 pKa = 4.18SLRR322 pKa = 11.84ADD324 pKa = 4.54LLPIKK329 pKa = 9.93NFPASYY335 pKa = 10.85EE336 pKa = 4.04NGKK339 pKa = 9.84LKK341 pKa = 10.73HH342 pKa = 6.17FLHH345 pKa = 6.92RR346 pKa = 11.84WMKK349 pKa = 10.66NRR351 pKa = 11.84IKK353 pKa = 11.09NNFWFTMFPEE363 pKa = 3.77KK364 pKa = 10.34RR365 pKa = 11.84YY366 pKa = 9.81FRR368 pKa = 11.84EE369 pKa = 4.23TTSTTEE375 pKa = 4.08VAIHH379 pKa = 6.02TNPLTDD385 pKa = 4.25LYY387 pKa = 11.56APIGTGNSRR396 pKa = 11.84TSGWYY401 pKa = 4.21TTIMKK406 pKa = 10.37LPFIFFIRR414 pKa = 11.84IGFMMASSGGSRR426 pKa = 11.84SLLRR430 pKa = 11.84QLQKK434 pKa = 11.34DD435 pKa = 3.29KK436 pKa = 11.26LRR438 pKa = 11.84WNRR441 pKa = 11.84EE442 pKa = 3.64SSVEE446 pKa = 4.14FIIAINARR454 pKa = 11.84SQSSGCDD461 pKa = 3.19ASRR464 pKa = 11.84HH465 pKa = 5.07FFDD468 pKa = 5.09GPRR471 pKa = 11.84PPGLPARR478 pKa = 11.84SEE480 pKa = 4.1EE481 pKa = 4.13FMGRR485 pKa = 11.84QAGEE489 pKa = 4.29TEE491 pKa = 3.96WAGTTNQAKK500 pKa = 9.82PSSRR504 pKa = 11.84RR505 pKa = 3.39

Molecular weight: 57.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

119

19

138

37120

13

2300

269.0

30.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.884 ± 0.43

1.272 ± 0.081

3.858 ± 0.192

5.0 ± 0.236

5.595 ± 0.238

6.915 ± 0.393

2.435 ± 0.14

8.314 ± 0.242

4.925 ± 0.386

10.291 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.077

4.34 ± 0.291

4.733 ± 0.221

3.44 ± 0.143

6.447 ± 0.268

7.977 ± 0.286

5.21 ± 0.141

5.722 ± 0.266

1.619 ± 0.124

3.559 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski