Bacillus phage Bp8p-T

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Agatevirus; Bacillus virus Bp8pC

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0PUM5|A0A0A0PUM5_9CAUD Uncharacterized protein OS=Bacillus phage Bp8p-T OX=1445811 GN=Bp8pT_040 PE=4 SV=1
MM1 pKa = 7.5SNSLVLKK8 pKa = 9.7MYY10 pKa = 10.53VDD12 pKa = 4.26KK13 pKa = 11.15SDD15 pKa = 3.19YY16 pKa = 9.78WDD18 pKa = 4.23YY19 pKa = 11.33ISEE22 pKa = 4.22NKK24 pKa = 9.91VISDD28 pKa = 4.55DD29 pKa = 5.05LYY31 pKa = 11.46CSDD34 pKa = 5.2IKK36 pKa = 11.44DD37 pKa = 3.52EE38 pKa = 4.64GDD40 pKa = 3.75DD41 pKa = 3.69YY42 pKa = 11.48TIAVYY47 pKa = 10.8AEE49 pKa = 4.44DD50 pKa = 4.79LSFAYY55 pKa = 10.31LVGNSACYY63 pKa = 10.38NIAKK67 pKa = 9.78GAEE70 pKa = 3.83ILDD73 pKa = 3.47RR74 pKa = 11.84SLRR77 pKa = 11.84EE78 pKa = 4.08DD79 pKa = 3.45EE80 pKa = 4.25

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0PUQ0|A0A0A0PUQ0_9CAUD Putative helicase OS=Bacillus phage Bp8p-T OX=1445811 GN=Bp8pT_095 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84KK3 pKa = 9.12PRR5 pKa = 11.84MRR7 pKa = 11.84AILKK11 pKa = 10.48AKK13 pKa = 9.42GCRR16 pKa = 11.84RR17 pKa = 11.84TSNSLFPRR25 pKa = 11.84VYY27 pKa = 10.9SNTKK31 pKa = 10.1RR32 pKa = 11.84VDD34 pKa = 3.34GEE36 pKa = 3.73IATIVQGGKK45 pKa = 9.77IINLFWVPKK54 pKa = 9.98ASLRR58 pKa = 11.84QHH60 pKa = 5.03YY61 pKa = 10.31RR62 pKa = 11.84EE63 pKa = 3.98VAKK66 pKa = 10.83ASTKK70 pKa = 10.59RR71 pKa = 11.84KK72 pKa = 9.83IGDD75 pKa = 3.27SRR77 pKa = 11.84LLKK80 pKa = 10.48KK81 pKa = 10.26RR82 pKa = 11.84VARR85 pKa = 11.84QMVYY89 pKa = 11.0LLVSEE94 pKa = 5.21DD95 pKa = 3.61SVEE98 pKa = 4.04RR99 pKa = 11.84CQLRR103 pKa = 11.84VKK105 pKa = 10.46NHH107 pKa = 5.9IYY109 pKa = 10.85EE110 pKa = 4.35NTEE113 pKa = 3.3EE114 pKa = 4.17AANKK118 pKa = 10.13LSQLNNKK125 pKa = 8.26EE126 pKa = 4.24RR127 pKa = 11.84LNKK130 pKa = 10.11

Molecular weight: 15.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

211

0

211

42750

37

1250

202.6

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.618 ± 0.211

0.713 ± 0.055

6.538 ± 0.149

8.255 ± 0.284

4.056 ± 0.096

5.939 ± 0.274

1.988 ± 0.107

6.292 ± 0.147

7.233 ± 0.214

8.344 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.098

5.207 ± 0.132

3.067 ± 0.18

3.775 ± 0.142

4.667 ± 0.127

6.526 ± 0.168

5.951 ± 0.228

7.006 ± 0.157

0.961 ± 0.072

4.271 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski