Amino acid dipepetide frequency for Bacillus phage Bp8p-T

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.152AlaAla: 6.152 ± 0.679
0.421AlaCys: 0.421 ± 0.111
4.515AlaAsp: 4.515 ± 0.3
5.263AlaGlu: 5.263 ± 0.451
2.55AlaPhe: 2.55 ± 0.234
4.07AlaGly: 4.07 ± 0.419
1.006AlaHis: 1.006 ± 0.143
4.398AlaIle: 4.398 ± 0.266
4.023AlaLys: 4.023 ± 0.35
5.497AlaLeu: 5.497 ± 0.398
1.614AlaMet: 1.614 ± 0.208
3.018AlaAsn: 3.018 ± 0.277
2.129AlaPro: 2.129 ± 0.603
2.667AlaGln: 2.667 ± 0.336
2.667AlaArg: 2.667 ± 0.267
3.696AlaSer: 3.696 ± 0.388
3.93AlaThr: 3.93 ± 0.486
5.287AlaVal: 5.287 ± 0.393
0.632AlaTrp: 0.632 ± 0.114
2.643AlaTyr: 2.643 ± 0.249
0.0AlaXaa: 0.0 ± 0.0
Cys
0.304CysAla: 0.304 ± 0.072
0.117CysCys: 0.117 ± 0.05
0.421CysAsp: 0.421 ± 0.112
0.515CysGlu: 0.515 ± 0.11
0.444CysPhe: 0.444 ± 0.093
0.351CysGly: 0.351 ± 0.098
0.257CysHis: 0.257 ± 0.079
0.257CysIle: 0.257 ± 0.076
0.702CysLys: 0.702 ± 0.127
0.632CysLeu: 0.632 ± 0.122
0.281CysMet: 0.281 ± 0.083
0.234CysAsn: 0.234 ± 0.076
0.398CysPro: 0.398 ± 0.107
0.094CysGln: 0.094 ± 0.041
0.304CysArg: 0.304 ± 0.097
0.561CysSer: 0.561 ± 0.13
0.374CysThr: 0.374 ± 0.103
0.421CysVal: 0.421 ± 0.099
0.094CysTrp: 0.094 ± 0.043
0.374CysTyr: 0.374 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.023AspAla: 4.023 ± 0.361
0.772AspCys: 0.772 ± 0.174
4.047AspAsp: 4.047 ± 0.324
4.889AspGlu: 4.889 ± 0.416
2.947AspPhe: 2.947 ± 0.294
3.79AspGly: 3.79 ± 0.444
1.263AspHis: 1.263 ± 0.194
4.749AspIle: 4.749 ± 0.297
4.538AspLys: 4.538 ± 0.394
5.216AspLeu: 5.216 ± 0.371
2.129AspMet: 2.129 ± 0.235
3.345AspAsn: 3.345 ± 0.293
1.895AspPro: 1.895 ± 0.215
2.199AspGln: 2.199 ± 0.208
2.924AspArg: 2.924 ± 0.271
3.883AspSer: 3.883 ± 0.344
3.649AspThr: 3.649 ± 0.272
5.029AspVal: 5.029 ± 0.314
0.725AspTrp: 0.725 ± 0.131
3.368AspTyr: 3.368 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
5.287GluAla: 5.287 ± 0.368
0.561GluCys: 0.561 ± 0.113
5.544GluAsp: 5.544 ± 0.489
9.521GluGlu: 9.521 ± 0.87
3.368GluPhe: 3.368 ± 0.325
4.866GluGly: 4.866 ± 0.386
1.591GluHis: 1.591 ± 0.207
5.076GluIle: 5.076 ± 0.403
5.755GluLys: 5.755 ± 0.425
8.164GluLeu: 8.164 ± 0.567
2.246GluMet: 2.246 ± 0.224
3.93GluAsn: 3.93 ± 0.301
1.661GluPro: 1.661 ± 0.217
2.994GluGln: 2.994 ± 0.266
3.579GluArg: 3.579 ± 0.321
4.491GluSer: 4.491 ± 0.316
3.602GluThr: 3.602 ± 0.304
5.988GluVal: 5.988 ± 0.387
1.076GluTrp: 1.076 ± 0.149
3.252GluTyr: 3.252 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
2.082PheAla: 2.082 ± 0.216
0.281PheCys: 0.281 ± 0.07
2.784PheAsp: 2.784 ± 0.231
3.018PheGlu: 3.018 ± 0.256
1.731PhePhe: 1.731 ± 0.218
2.152PheGly: 2.152 ± 0.256
1.029PheHis: 1.029 ± 0.17
2.784PheIle: 2.784 ± 0.296
2.924PheLys: 2.924 ± 0.248
3.018PheLeu: 3.018 ± 0.317
1.053PheMet: 1.053 ± 0.147
2.807PheAsn: 2.807 ± 0.254
1.287PhePro: 1.287 ± 0.196
1.333PheGln: 1.333 ± 0.2
1.684PheArg: 1.684 ± 0.24
3.158PheSer: 3.158 ± 0.294
2.714PheThr: 2.714 ± 0.283
2.737PheVal: 2.737 ± 0.268
0.257PheTrp: 0.257 ± 0.094
1.731PheTyr: 1.731 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
4.023GlyAla: 4.023 ± 0.546
0.538GlyCys: 0.538 ± 0.127
3.462GlyAsp: 3.462 ± 0.328
4.234GlyGlu: 4.234 ± 0.308
2.643GlyPhe: 2.643 ± 0.212
4.866GlyGly: 4.866 ± 0.781
1.216GlyHis: 1.216 ± 0.151
4.047GlyIle: 4.047 ± 0.417
4.678GlyLys: 4.678 ± 0.335
4.14GlyLeu: 4.14 ± 0.334
1.708GlyMet: 1.708 ± 0.231
3.252GlyAsn: 3.252 ± 0.241
0.281GlyPro: 0.281 ± 0.066
2.059GlyGln: 2.059 ± 0.248
2.737GlyArg: 2.737 ± 0.233
3.79GlySer: 3.79 ± 0.425
3.696GlyThr: 3.696 ± 0.398
4.585GlyVal: 4.585 ± 0.31
0.749GlyTrp: 0.749 ± 0.162
2.69GlyTyr: 2.69 ± 0.247
0.0GlyXaa: 0.0 ± 0.0
His
1.193HisAla: 1.193 ± 0.17
0.117HisCys: 0.117 ± 0.051
1.333HisAsp: 1.333 ± 0.188
1.357HisGlu: 1.357 ± 0.2
0.912HisPhe: 0.912 ± 0.145
1.123HisGly: 1.123 ± 0.16
0.515HisHis: 0.515 ± 0.124
1.637HisIle: 1.637 ± 0.225
1.474HisLys: 1.474 ± 0.237
1.731HisLeu: 1.731 ± 0.202
0.374HisMet: 0.374 ± 0.094
0.982HisAsn: 0.982 ± 0.162
0.585HisPro: 0.585 ± 0.108
0.327HisGln: 0.327 ± 0.08
0.889HisArg: 0.889 ± 0.119
1.333HisSer: 1.333 ± 0.172
1.099HisThr: 1.099 ± 0.178
1.333HisVal: 1.333 ± 0.152
0.281HisTrp: 0.281 ± 0.078
1.287HisTyr: 1.287 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.515IleAla: 4.515 ± 0.284
0.561IleCys: 0.561 ± 0.143
5.006IleAsp: 5.006 ± 0.304
5.146IleGlu: 5.146 ± 0.397
2.152IlePhe: 2.152 ± 0.216
3.579IleGly: 3.579 ± 0.335
1.123IleHis: 1.123 ± 0.174
3.532IleIle: 3.532 ± 0.297
4.491IleLys: 4.491 ± 0.336
4.795IleLeu: 4.795 ± 0.29
1.357IleMet: 1.357 ± 0.171
3.228IleAsn: 3.228 ± 0.281
2.059IlePro: 2.059 ± 0.202
2.573IleGln: 2.573 ± 0.238
3.228IleArg: 3.228 ± 0.255
4.257IleSer: 4.257 ± 0.297
4.398IleThr: 4.398 ± 0.396
4.304IleVal: 4.304 ± 0.28
0.327IleTrp: 0.327 ± 0.079
2.292IleTyr: 2.292 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
5.333LysAla: 5.333 ± 0.366
0.351LysCys: 0.351 ± 0.118
5.053LysAsp: 5.053 ± 0.36
7.556LysGlu: 7.556 ± 0.554
2.947LysPhe: 2.947 ± 0.273
3.836LysGly: 3.836 ± 0.294
1.357LysHis: 1.357 ± 0.179
4.211LysIle: 4.211 ± 0.354
6.035LysLys: 6.035 ± 0.482
5.45LysLeu: 5.45 ± 0.386
2.082LysMet: 2.082 ± 0.239
3.345LysAsn: 3.345 ± 0.26
2.199LysPro: 2.199 ± 0.243
2.994LysGln: 2.994 ± 0.271
3.836LysArg: 3.836 ± 0.331
4.257LysSer: 4.257 ± 0.355
3.439LysThr: 3.439 ± 0.305
4.585LysVal: 4.585 ± 0.362
0.655LysTrp: 0.655 ± 0.134
2.807LysTyr: 2.807 ± 0.264
0.0LysXaa: 0.0 ± 0.0
Leu
5.708LeuAla: 5.708 ± 0.359
0.444LeuCys: 0.444 ± 0.109
5.357LeuAsp: 5.357 ± 0.305
6.924LeuGlu: 6.924 ± 0.525
3.018LeuPhe: 3.018 ± 0.281
4.374LeuGly: 4.374 ± 0.371
2.035LeuHis: 2.035 ± 0.266
4.398LeuIle: 4.398 ± 0.321
6.222LeuLys: 6.222 ± 0.358
6.784LeuLeu: 6.784 ± 0.496
2.246LeuMet: 2.246 ± 0.203
4.678LeuAsn: 4.678 ± 0.355
3.228LeuPro: 3.228 ± 0.26
3.556LeuGln: 3.556 ± 0.338
4.14LeuArg: 4.14 ± 0.299
5.708LeuSer: 5.708 ± 0.335
5.755LeuThr: 5.755 ± 0.419
5.193LeuVal: 5.193 ± 0.299
0.585LeuTrp: 0.585 ± 0.111
3.088LeuTyr: 3.088 ± 0.273
0.0LeuXaa: 0.0 ± 0.0
Met
1.333MetAla: 1.333 ± 0.191
0.14MetCys: 0.14 ± 0.051
1.661MetAsp: 1.661 ± 0.218
1.965MetGlu: 1.965 ± 0.215
0.795MetPhe: 0.795 ± 0.146
1.24MetGly: 1.24 ± 0.174
0.468MetHis: 0.468 ± 0.093
1.661MetIle: 1.661 ± 0.189
2.129MetLys: 2.129 ± 0.261
2.082MetLeu: 2.082 ± 0.221
0.795MetMet: 0.795 ± 0.121
1.731MetAsn: 1.731 ± 0.193
1.029MetPro: 1.029 ± 0.182
1.146MetGln: 1.146 ± 0.186
1.17MetArg: 1.17 ± 0.16
1.848MetSer: 1.848 ± 0.239
1.801MetThr: 1.801 ± 0.155
1.333MetVal: 1.333 ± 0.191
0.374MetTrp: 0.374 ± 0.087
1.216MetTyr: 1.216 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
2.737AsnAla: 2.737 ± 0.245
0.257AsnCys: 0.257 ± 0.069
3.088AsnAsp: 3.088 ± 0.251
3.556AsnGlu: 3.556 ± 0.313
2.059AsnPhe: 2.059 ± 0.249
3.93AsnGly: 3.93 ± 0.438
1.006AsnHis: 1.006 ± 0.198
3.415AsnIle: 3.415 ± 0.302
3.626AsnLys: 3.626 ± 0.246
4.468AsnLeu: 4.468 ± 0.363
1.24AsnMet: 1.24 ± 0.144
3.088AsnAsn: 3.088 ± 0.322
2.597AsnPro: 2.597 ± 0.228
1.871AsnGln: 1.871 ± 0.252
2.714AsnArg: 2.714 ± 0.278
3.579AsnSer: 3.579 ± 0.274
2.924AsnThr: 2.924 ± 0.251
3.252AsnVal: 3.252 ± 0.284
0.538AsnTrp: 0.538 ± 0.095
2.105AsnTyr: 2.105 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
2.854ProAla: 2.854 ± 0.646
0.117ProCys: 0.117 ± 0.057
1.801ProAsp: 1.801 ± 0.236
2.292ProGlu: 2.292 ± 0.223
1.497ProPhe: 1.497 ± 0.213
0.982ProGly: 0.982 ± 0.144
0.795ProHis: 0.795 ± 0.156
1.965ProIle: 1.965 ± 0.186
1.731ProLys: 1.731 ± 0.195
2.924ProLeu: 2.924 ± 0.274
0.702ProMet: 0.702 ± 0.154
1.754ProAsn: 1.754 ± 0.182
1.123ProPro: 1.123 ± 0.243
1.17ProGln: 1.17 ± 0.188
1.053ProArg: 1.053 ± 0.181
2.105ProSer: 2.105 ± 0.248
2.246ProThr: 2.246 ± 0.289
1.754ProVal: 1.754 ± 0.2
0.117ProTrp: 0.117 ± 0.05
1.684ProTyr: 1.684 ± 0.206
0.0ProXaa: 0.0 ± 0.0
Gln
2.409GlnAla: 2.409 ± 0.223
0.187GlnCys: 0.187 ± 0.066
2.222GlnAsp: 2.222 ± 0.265
3.719GlnGlu: 3.719 ± 0.288
1.38GlnPhe: 1.38 ± 0.167
2.175GlnGly: 2.175 ± 0.266
0.585GlnHis: 0.585 ± 0.099
2.199GlnIle: 2.199 ± 0.236
2.48GlnLys: 2.48 ± 0.288
3.977GlnLeu: 3.977 ± 0.262
1.146GlnMet: 1.146 ± 0.167
1.731GlnAsn: 1.731 ± 0.22
1.123GlnPro: 1.123 ± 0.239
2.012GlnGln: 2.012 ± 0.264
1.637GlnArg: 1.637 ± 0.218
2.456GlnSer: 2.456 ± 0.242
1.871GlnThr: 1.871 ± 0.242
2.456GlnVal: 2.456 ± 0.27
0.491GlnTrp: 0.491 ± 0.102
1.497GlnTyr: 1.497 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
2.737ArgAla: 2.737 ± 0.227
0.327ArgCys: 0.327 ± 0.084
3.158ArgAsp: 3.158 ± 0.287
3.907ArgGlu: 3.907 ± 0.344
1.825ArgPhe: 1.825 ± 0.162
2.667ArgGly: 2.667 ± 0.303
1.006ArgHis: 1.006 ± 0.131
3.322ArgIle: 3.322 ± 0.281
3.883ArgLys: 3.883 ± 0.365
4.211ArgLeu: 4.211 ± 0.335
1.38ArgMet: 1.38 ± 0.207
2.222ArgAsn: 2.222 ± 0.228
0.982ArgPro: 0.982 ± 0.135
1.825ArgGln: 1.825 ± 0.217
2.246ArgArg: 2.246 ± 0.227
2.526ArgSer: 2.526 ± 0.259
2.526ArgThr: 2.526 ± 0.239
3.626ArgVal: 3.626 ± 0.308
0.281ArgTrp: 0.281 ± 0.083
2.012ArgTyr: 2.012 ± 0.224
0.0ArgXaa: 0.0 ± 0.0
Ser
4.187SerAla: 4.187 ± 0.417
0.538SerCys: 0.538 ± 0.122
3.743SerAsp: 3.743 ± 0.368
4.211SerGlu: 4.211 ± 0.317
3.111SerPhe: 3.111 ± 0.303
4.211SerGly: 4.211 ± 0.496
0.936SerHis: 0.936 ± 0.166
4.164SerIle: 4.164 ± 0.307
4.678SerLys: 4.678 ± 0.363
5.871SerLeu: 5.871 ± 0.374
1.965SerMet: 1.965 ± 0.199
3.439SerAsn: 3.439 ± 0.354
1.942SerPro: 1.942 ± 0.234
2.386SerGln: 2.386 ± 0.241
3.509SerArg: 3.509 ± 0.295
5.076SerSer: 5.076 ± 0.425
4.094SerThr: 4.094 ± 0.409
3.883SerVal: 3.883 ± 0.28
0.702SerTrp: 0.702 ± 0.128
2.62SerTyr: 2.62 ± 0.258
0.0SerXaa: 0.0 ± 0.0
Thr
3.93ThrAla: 3.93 ± 0.428
0.491ThrCys: 0.491 ± 0.114
3.907ThrAsp: 3.907 ± 0.304
4.749ThrGlu: 4.749 ± 0.365
2.386ThrPhe: 2.386 ± 0.248
4.023ThrGly: 4.023 ± 0.42
1.053ThrHis: 1.053 ± 0.155
3.532ThrIle: 3.532 ± 0.366
4.117ThrLys: 4.117 ± 0.269
4.351ThrLeu: 4.351 ± 0.315
1.006ThrMet: 1.006 ± 0.153
2.877ThrAsn: 2.877 ± 0.313
2.269ThrPro: 2.269 ± 0.293
2.339ThrGln: 2.339 ± 0.244
2.69ThrArg: 2.69 ± 0.231
3.836ThrSer: 3.836 ± 0.408
3.556ThrThr: 3.556 ± 0.382
5.497ThrVal: 5.497 ± 0.521
0.398ThrTrp: 0.398 ± 0.095
2.503ThrTyr: 2.503 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
4.047ValAla: 4.047 ± 0.322
0.491ValCys: 0.491 ± 0.106
4.725ValAsp: 4.725 ± 0.352
5.404ValGlu: 5.404 ± 0.44
3.111ValPhe: 3.111 ± 0.356
3.696ValGly: 3.696 ± 0.278
1.567ValHis: 1.567 ± 0.176
4.211ValIle: 4.211 ± 0.286
5.31ValLys: 5.31 ± 0.393
5.24ValLeu: 5.24 ± 0.376
1.404ValMet: 1.404 ± 0.188
3.322ValAsn: 3.322 ± 0.285
2.643ValPro: 2.643 ± 0.259
2.456ValGln: 2.456 ± 0.237
3.345ValArg: 3.345 ± 0.329
5.216ValSer: 5.216 ± 0.378
4.678ValThr: 4.678 ± 0.398
5.123ValVal: 5.123 ± 0.451
0.655ValTrp: 0.655 ± 0.131
3.415ValTyr: 3.415 ± 0.323
0.0ValXaa: 0.0 ± 0.0
Trp
0.632TrpAla: 0.632 ± 0.114
0.047TrpCys: 0.047 ± 0.031
0.678TrpAsp: 0.678 ± 0.127
0.936TrpGlu: 0.936 ± 0.139
0.351TrpPhe: 0.351 ± 0.095
0.608TrpGly: 0.608 ± 0.114
0.281TrpHis: 0.281 ± 0.081
0.561TrpIle: 0.561 ± 0.113
0.866TrpLys: 0.866 ± 0.134
0.819TrpLeu: 0.819 ± 0.155
0.187TrpMet: 0.187 ± 0.065
0.678TrpAsn: 0.678 ± 0.138
0.0TrpPro: 0.0 ± 0.0
0.234TrpGln: 0.234 ± 0.063
0.211TrpArg: 0.211 ± 0.066
0.561TrpSer: 0.561 ± 0.119
0.468TrpThr: 0.468 ± 0.097
0.749TrpVal: 0.749 ± 0.129
0.234TrpTrp: 0.234 ± 0.087
0.515TrpTyr: 0.515 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.69TyrAla: 2.69 ± 0.204
0.374TyrCys: 0.374 ± 0.094
2.877TyrAsp: 2.877 ± 0.26
3.392TyrGlu: 3.392 ± 0.237
1.333TyrPhe: 1.333 ± 0.167
2.854TyrGly: 2.854 ± 0.309
0.795TyrHis: 0.795 ± 0.133
2.807TyrIle: 2.807 ± 0.251
2.667TyrLys: 2.667 ± 0.211
4.07TyrLeu: 4.07 ± 0.3
0.982TyrMet: 0.982 ± 0.132
2.409TyrAsn: 2.409 ± 0.238
1.24TyrPro: 1.24 ± 0.16
1.521TyrGln: 1.521 ± 0.16
2.105TyrArg: 2.105 ± 0.199
2.924TyrSer: 2.924 ± 0.229
2.69TyrThr: 2.69 ± 0.26
2.924TyrVal: 2.924 ± 0.303
0.444TyrTrp: 0.444 ± 0.102
1.614TyrTyr: 1.614 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 211 proteins (42750 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski