Salmonella phage 103203_sal5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192Y6J9|A0A192Y6J9_9CAUD Arc domain-containing protein OS=Salmonella phage 103203_sal5 OX=1813779 PE=4 SV=1
MM1 pKa = 7.65CEE3 pKa = 3.94DD4 pKa = 3.59EE5 pKa = 5.33EE6 pKa = 4.43GRR8 pKa = 11.84EE9 pKa = 4.1YY10 pKa = 11.29NSSNEE15 pKa = 3.93FSCGRR20 pKa = 11.84FGVPLYY26 pKa = 10.16ASPPAPVVPEE36 pKa = 4.84DD37 pKa = 3.09ISGIIEE43 pKa = 4.34RR44 pKa = 11.84FQYY47 pKa = 10.14QADD50 pKa = 3.85HH51 pKa = 6.52LSDD54 pKa = 2.88WRR56 pKa = 11.84HH57 pKa = 5.7IDD59 pKa = 3.31EE60 pKa = 5.3HH61 pKa = 6.31SCKK64 pKa = 10.33VNRR67 pKa = 11.84RR68 pKa = 11.84DD69 pKa = 4.65LMTALEE75 pKa = 4.57FMDD78 pKa = 3.65SCRR81 pKa = 11.84AAMLQGKK88 pKa = 8.65FRR90 pKa = 11.84DD91 pKa = 3.59LSQPVDD97 pKa = 3.76PQVADD102 pKa = 3.53YY103 pKa = 10.02EE104 pKa = 4.51KK105 pKa = 10.69TMLQAGNSPVTPGGWISCSEE125 pKa = 4.02RR126 pKa = 11.84MPAQDD131 pKa = 4.19DD132 pKa = 3.67WVLIYY137 pKa = 10.75SKK139 pKa = 10.8YY140 pKa = 10.76GEE142 pKa = 4.23YY143 pKa = 10.67LAGQVQGEE151 pKa = 4.38YY152 pKa = 10.95VEE154 pKa = 5.85LNDD157 pKa = 5.49GTLSWLGAALHH168 pKa = 6.09WMPLPEE174 pKa = 5.3PPQQQ178 pKa = 3.34

Molecular weight: 20.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192Y629|A0A192Y629_9CAUD Repressor OS=Salmonella phage 103203_sal5 OX=1813779 PE=4 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84TQFKK8 pKa = 7.85GTSRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.84RR18 pKa = 11.84LKK20 pKa = 11.2AKK22 pKa = 10.38ALANGVLARR31 pKa = 11.84EE32 pKa = 4.08EE33 pKa = 4.91AITSEE38 pKa = 4.28VLHH41 pKa = 6.71RR42 pKa = 11.84PTLSRR47 pKa = 11.84AQIQAKK53 pKa = 9.67GKK55 pKa = 9.64HH56 pKa = 4.86EE57 pKa = 4.14TPKK60 pKa = 10.42RR61 pKa = 11.84IEE63 pKa = 4.21DD64 pKa = 3.59AKK66 pKa = 11.04SLQFMAKK73 pKa = 9.92DD74 pKa = 3.48AFWQLEE80 pKa = 4.22EE81 pKa = 3.9YY82 pKa = 10.34RR83 pKa = 11.84RR84 pKa = 11.84NLEE87 pKa = 3.59RR88 pKa = 11.84AAIVYY93 pKa = 10.58ANEE96 pKa = 4.43FGHH99 pKa = 6.59KK100 pKa = 9.71PPEE103 pKa = 4.36TGVCLPDD110 pKa = 3.32VALYY114 pKa = 10.48AAGHH118 pKa = 6.21RR119 pKa = 11.84KK120 pKa = 9.32SKK122 pKa = 10.8QITARR127 pKa = 3.53

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12172

36

725

202.9

22.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.029 ± 0.513

1.06 ± 0.128

5.825 ± 0.297

6.342 ± 0.351

3.713 ± 0.336

7.172 ± 0.404

1.635 ± 0.154

6.416 ± 0.503

6.186 ± 0.294

7.969 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.073 ± 0.169

4.831 ± 0.285

4.083 ± 0.177

4.527 ± 0.407

5.891 ± 0.302

6.236 ± 0.273

5.02 ± 0.297

6.17 ± 0.264

1.528 ± 0.171

3.294 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski