Lactococcus phage 949

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; Lactococcus virus 949

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0YIT9|E0YIT9_9CAUD Putative nucleotidase OS=Lactococcus phage 949 OX=881953 PE=4 SV=1
MM1 pKa = 7.58LMQYY5 pKa = 8.86TGRR8 pKa = 11.84DD9 pKa = 3.03GDD11 pKa = 4.8LGLKK15 pKa = 9.16TNEE18 pKa = 3.82NYY20 pKa = 10.57YY21 pKa = 10.82VVATTDD27 pKa = 3.01HH28 pKa = 6.42NMWSICVYY36 pKa = 9.94IYY38 pKa = 9.76EE39 pKa = 4.58INSTYY44 pKa = 10.49PIFCPYY50 pKa = 9.83DD51 pKa = 3.39TFEE54 pKa = 4.38GFFMNWNPHH63 pKa = 5.07PKK65 pKa = 9.17IEE67 pKa = 4.09EE68 pKa = 4.08EE69 pKa = 4.28EE70 pKa = 4.17IEE72 pKa = 5.43EE73 pKa = 4.08EE74 pKa = 4.99DD75 pKa = 4.06DD76 pKa = 3.53WSDD79 pKa = 3.41FF80 pKa = 3.83

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0YIY1|E0YIY1_9CAUD DNA topoisomerase (ATP-hydrolyzing) OS=Lactococcus phage 949 OX=881953 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84KK3 pKa = 8.96MSDD6 pKa = 2.86SHH8 pKa = 8.05IKK10 pKa = 7.63TTHH13 pKa = 5.68QNTTLYY19 pKa = 10.74SILAGNLGGNRR30 pKa = 11.84YY31 pKa = 9.38RR32 pKa = 11.84DD33 pKa = 3.33GKK35 pKa = 10.6SEE37 pKa = 3.88NDD39 pKa = 3.48RR40 pKa = 11.84EE41 pKa = 4.48GVVCLSARR49 pKa = 11.84VFKK52 pKa = 10.82KK53 pKa = 10.58LSFYY57 pKa = 9.91TGWVARR63 pKa = 11.84RR64 pKa = 11.84HH65 pKa = 5.61ASFISS70 pKa = 3.33

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

33506

29

3276

217.6

24.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.999 ± 0.379

0.606 ± 0.091

6.82 ± 0.17

7.915 ± 0.264

4.507 ± 0.237

5.715 ± 0.258

1.456 ± 0.112

7.682 ± 0.194

9.3 ± 0.203

8.148 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.118

6.408 ± 0.17

2.253 ± 0.107

3.378 ± 0.279

3.501 ± 0.163

6.802 ± 0.286

6.053 ± 0.224

6.259 ± 0.153

1.027 ± 0.088

4.614 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski