Amino acid dipepetide frequency for Lactococcus phage 949

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.149AlaAla: 0.149 ± 0.071
0.239AlaCys: 0.239 ± 0.082
2.865AlaAsp: 2.865 ± 0.336
2.865AlaGlu: 2.865 ± 0.282
1.85AlaPhe: 1.85 ± 0.203
3.79AlaGly: 3.79 ± 0.706
0.686AlaHis: 0.686 ± 0.146
4.089AlaIle: 4.089 ± 0.412
5.551AlaLys: 5.551 ± 0.578
4.686AlaLeu: 4.686 ± 0.533
1.433AlaMet: 1.433 ± 0.323
3.044AlaAsn: 3.044 ± 0.369
1.134AlaPro: 1.134 ± 0.228
2.716AlaGln: 2.716 ± 0.452
1.731AlaArg: 1.731 ± 0.26
3.432AlaSer: 3.432 ± 0.454
3.074AlaThr: 3.074 ± 0.4
3.462AlaVal: 3.462 ± 0.36
0.507AlaTrp: 0.507 ± 0.115
2.686AlaTyr: 2.686 ± 0.298
0.0AlaXaa: 0.0 ± 0.0
Cys
0.209CysAla: 0.209 ± 0.084
0.06CysCys: 0.06 ± 0.043
0.597CysAsp: 0.597 ± 0.159
0.239CysGlu: 0.239 ± 0.101
0.448CysPhe: 0.448 ± 0.117
0.597CysGly: 0.597 ± 0.172
0.03CysHis: 0.03 ± 0.03
0.388CysIle: 0.388 ± 0.115
0.507CysLys: 0.507 ± 0.188
0.418CysLeu: 0.418 ± 0.112
0.06CysMet: 0.06 ± 0.037
0.388CysAsn: 0.388 ± 0.099
0.269CysPro: 0.269 ± 0.11
0.149CysGln: 0.149 ± 0.066
0.298CysArg: 0.298 ± 0.085
0.418CysSer: 0.418 ± 0.11
0.239CysThr: 0.239 ± 0.085
0.388CysVal: 0.388 ± 0.113
0.09CysTrp: 0.09 ± 0.052
0.269CysTyr: 0.269 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
2.656AspAla: 2.656 ± 0.41
0.269AspCys: 0.269 ± 0.088
4.626AspAsp: 4.626 ± 0.371
5.91AspGlu: 5.91 ± 0.473
3.97AspPhe: 3.97 ± 0.253
4.596AspGly: 4.596 ± 0.45
0.567AspHis: 0.567 ± 0.151
5.91AspIle: 5.91 ± 0.434
6.745AspLys: 6.745 ± 0.488
6.059AspLeu: 6.059 ± 0.521
1.91AspMet: 1.91 ± 0.251
4.686AspAsn: 4.686 ± 0.298
0.925AspPro: 0.925 ± 0.173
0.955AspGln: 0.955 ± 0.236
2.268AspArg: 2.268 ± 0.301
4.984AspSer: 4.984 ± 0.437
3.552AspThr: 3.552 ± 0.353
3.701AspVal: 3.701 ± 0.332
0.806AspTrp: 0.806 ± 0.154
3.074AspTyr: 3.074 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
4.208GluAla: 4.208 ± 0.516
0.388GluCys: 0.388 ± 0.122
5.88GluAsp: 5.88 ± 0.631
6.835GluGlu: 6.835 ± 0.717
3.074GluPhe: 3.074 ± 0.369
3.432GluGly: 3.432 ± 0.324
1.313GluHis: 1.313 ± 0.297
6.686GluIle: 6.686 ± 0.442
7.312GluLys: 7.312 ± 0.714
7.521GluLeu: 7.521 ± 0.562
2.03GluMet: 2.03 ± 0.26
5.104GluAsn: 5.104 ± 0.439
0.955GluPro: 0.955 ± 0.196
2.418GluGln: 2.418 ± 0.258
2.567GluArg: 2.567 ± 0.31
5.342GluSer: 5.342 ± 0.383
4.029GluThr: 4.029 ± 0.341
5.044GluVal: 5.044 ± 0.371
0.836GluTrp: 0.836 ± 0.131
4.178GluTyr: 4.178 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
2.209PheAla: 2.209 ± 0.267
0.388PheCys: 0.388 ± 0.134
3.761PheAsp: 3.761 ± 0.378
3.82PheGlu: 3.82 ± 0.408
1.283PhePhe: 1.283 ± 0.25
2.865PheGly: 2.865 ± 0.296
0.537PheHis: 0.537 ± 0.137
3.223PheIle: 3.223 ± 0.374
4.178PheLys: 4.178 ± 0.308
3.283PheLeu: 3.283 ± 0.425
1.522PheMet: 1.522 ± 0.256
3.432PheAsn: 3.432 ± 0.29
0.746PhePro: 0.746 ± 0.164
1.373PheGln: 1.373 ± 0.179
1.612PheArg: 1.612 ± 0.255
3.104PheSer: 3.104 ± 0.375
2.865PheThr: 2.865 ± 0.283
2.388PheVal: 2.388 ± 0.288
0.358PheTrp: 0.358 ± 0.1
2.119PheTyr: 2.119 ± 0.329
0.0PheXaa: 0.0 ± 0.0
Gly
2.776GlyAla: 2.776 ± 0.555
0.448GlyCys: 0.448 ± 0.108
3.313GlyAsp: 3.313 ± 0.361
4.417GlyGlu: 4.417 ± 0.353
3.402GlyPhe: 3.402 ± 0.323
3.373GlyGly: 3.373 ± 0.592
0.985GlyHis: 0.985 ± 0.192
4.775GlyIle: 4.775 ± 0.414
4.925GlyLys: 4.925 ± 0.439
4.596GlyLeu: 4.596 ± 0.385
1.522GlyMet: 1.522 ± 0.227
3.999GlyAsn: 3.999 ± 0.457
0.0GlyPro: 0.0 ± 0.0
1.462GlyGln: 1.462 ± 0.219
1.97GlyArg: 1.97 ± 0.231
3.492GlySer: 3.492 ± 0.383
3.91GlyThr: 3.91 ± 0.357
3.641GlyVal: 3.641 ± 0.357
0.776GlyTrp: 0.776 ± 0.211
3.373GlyTyr: 3.373 ± 0.347
0.0GlyXaa: 0.0 ± 0.0
His
0.716HisAla: 0.716 ± 0.143
0.09HisCys: 0.09 ± 0.05
1.254HisAsp: 1.254 ± 0.177
0.836HisGlu: 0.836 ± 0.202
0.686HisPhe: 0.686 ± 0.161
0.985HisGly: 0.985 ± 0.198
0.239HisHis: 0.239 ± 0.105
1.552HisIle: 1.552 ± 0.265
1.134HisLys: 1.134 ± 0.176
1.104HisLeu: 1.104 ± 0.213
0.298HisMet: 0.298 ± 0.112
0.895HisAsn: 0.895 ± 0.204
0.388HisPro: 0.388 ± 0.12
0.507HisGln: 0.507 ± 0.126
0.478HisArg: 0.478 ± 0.11
1.074HisSer: 1.074 ± 0.174
0.836HisThr: 0.836 ± 0.148
0.567HisVal: 0.567 ± 0.124
0.149HisTrp: 0.149 ± 0.063
0.776HisTyr: 0.776 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
3.97IleAla: 3.97 ± 0.316
0.478IleCys: 0.478 ± 0.144
5.462IleAsp: 5.462 ± 0.39
6.715IleGlu: 6.715 ± 0.542
3.313IlePhe: 3.313 ± 0.404
3.373IleGly: 3.373 ± 0.344
0.895IleHis: 0.895 ± 0.155
5.522IleIle: 5.522 ± 0.534
7.641IleLys: 7.641 ± 0.451
5.79IleLeu: 5.79 ± 0.513
1.552IleMet: 1.552 ± 0.212
5.79IleAsn: 5.79 ± 0.491
2.716IlePro: 2.716 ± 0.287
2.626IleGln: 2.626 ± 0.41
2.388IleArg: 2.388 ± 0.244
5.462IleSer: 5.462 ± 0.435
4.298IleThr: 4.298 ± 0.43
5.283IleVal: 5.283 ± 0.497
0.746IleTrp: 0.746 ± 0.154
2.806IleTyr: 2.806 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
5.044LysAla: 5.044 ± 0.515
0.567LysCys: 0.567 ± 0.153
6.656LysAsp: 6.656 ± 0.418
9.521LysGlu: 9.521 ± 0.651
3.82LysPhe: 3.82 ± 0.472
4.358LysGly: 4.358 ± 0.422
1.433LysHis: 1.433 ± 0.22
6.924LysIle: 6.924 ± 0.462
8.417LysLys: 8.417 ± 0.614
8.148LysLeu: 8.148 ± 0.484
2.746LysMet: 2.746 ± 0.365
5.611LysAsn: 5.611 ± 0.437
2.209LysPro: 2.209 ± 0.285
3.223LysGln: 3.223 ± 0.406
3.671LysArg: 3.671 ± 0.383
4.716LysSer: 4.716 ± 0.443
5.581LysThr: 5.581 ± 0.399
5.432LysVal: 5.432 ± 0.378
1.104LysTrp: 1.104 ± 0.184
3.82LysTyr: 3.82 ± 0.329
0.0LysXaa: 0.0 ± 0.0
Leu
4.746LeuAla: 4.746 ± 0.422
0.537LeuCys: 0.537 ± 0.144
5.939LeuAsp: 5.939 ± 0.45
5.88LeuGlu: 5.88 ± 0.416
3.641LeuPhe: 3.641 ± 0.438
5.313LeuGly: 5.313 ± 0.503
0.925LeuHis: 0.925 ± 0.168
5.402LeuIle: 5.402 ± 0.452
7.999LeuLys: 7.999 ± 0.519
6.238LeuLeu: 6.238 ± 0.461
1.731LeuMet: 1.731 ± 0.184
5.462LeuAsn: 5.462 ± 0.461
2.268LeuPro: 2.268 ± 0.273
2.686LeuGln: 2.686 ± 0.403
2.716LeuArg: 2.716 ± 0.305
6.686LeuSer: 6.686 ± 0.559
5.163LeuThr: 5.163 ± 0.36
4.268LeuVal: 4.268 ± 0.453
0.657LeuTrp: 0.657 ± 0.143
3.223LeuTyr: 3.223 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
1.94MetAla: 1.94 ± 0.216
0.03MetCys: 0.03 ± 0.031
1.791MetAsp: 1.791 ± 0.268
1.761MetGlu: 1.761 ± 0.236
1.015MetPhe: 1.015 ± 0.193
1.254MetGly: 1.254 ± 0.208
0.179MetHis: 0.179 ± 0.077
1.791MetIle: 1.791 ± 0.247
2.209MetLys: 2.209 ± 0.275
1.821MetLeu: 1.821 ± 0.248
0.627MetMet: 0.627 ± 0.136
2.03MetAsn: 2.03 ± 0.248
0.657MetPro: 0.657 ± 0.126
1.045MetGln: 1.045 ± 0.301
0.806MetArg: 0.806 ± 0.168
1.88MetSer: 1.88 ± 0.289
1.85MetThr: 1.85 ± 0.243
1.642MetVal: 1.642 ± 0.207
0.239MetTrp: 0.239 ± 0.092
1.015MetTyr: 1.015 ± 0.182
0.0MetXaa: 0.0 ± 0.0
Asn
3.373AsnAla: 3.373 ± 0.354
0.478AsnCys: 0.478 ± 0.147
3.313AsnAsp: 3.313 ± 0.363
5.044AsnGlu: 5.044 ± 0.38
2.895AsnPhe: 2.895 ± 0.271
4.387AsnGly: 4.387 ± 0.363
1.015AsnHis: 1.015 ± 0.206
5.044AsnIle: 5.044 ± 0.439
6.327AsnLys: 6.327 ± 0.437
5.223AsnLeu: 5.223 ± 0.393
1.492AsnMet: 1.492 ± 0.193
3.611AsnAsn: 3.611 ± 0.437
2.0AsnPro: 2.0 ± 0.273
2.746AsnGln: 2.746 ± 0.359
2.03AsnArg: 2.03 ± 0.285
4.477AsnSer: 4.477 ± 0.392
3.253AsnThr: 3.253 ± 0.273
3.999AsnVal: 3.999 ± 0.303
0.507AsnTrp: 0.507 ± 0.123
2.865AsnTyr: 2.865 ± 0.298
0.0AsnXaa: 0.0 ± 0.0
Pro
1.134ProAla: 1.134 ± 0.182
0.119ProCys: 0.119 ± 0.072
1.701ProAsp: 1.701 ± 0.226
1.731ProGlu: 1.731 ± 0.209
1.254ProPhe: 1.254 ± 0.205
0.239ProGly: 0.239 ± 0.087
0.448ProHis: 0.448 ± 0.113
1.462ProIle: 1.462 ± 0.233
2.059ProLys: 2.059 ± 0.29
1.88ProLeu: 1.88 ± 0.271
0.627ProMet: 0.627 ± 0.154
1.612ProAsn: 1.612 ± 0.21
0.328ProPro: 0.328 ± 0.09
0.627ProGln: 0.627 ± 0.13
0.686ProArg: 0.686 ± 0.16
1.791ProSer: 1.791 ± 0.252
1.582ProThr: 1.582 ± 0.226
2.119ProVal: 2.119 ± 0.343
0.09ProTrp: 0.09 ± 0.056
1.045ProTyr: 1.045 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
2.149GlnAla: 2.149 ± 0.417
0.179GlnCys: 0.179 ± 0.08
1.403GlnAsp: 1.403 ± 0.187
2.418GlnGlu: 2.418 ± 0.244
1.194GlnPhe: 1.194 ± 0.195
1.701GlnGly: 1.701 ± 0.215
0.746GlnHis: 0.746 ± 0.179
2.447GlnIle: 2.447 ± 0.291
3.014GlnLys: 3.014 ± 0.485
3.402GlnLeu: 3.402 ± 0.497
1.343GlnMet: 1.343 ± 0.242
2.119GlnAsn: 2.119 ± 0.236
0.776GlnPro: 0.776 ± 0.153
1.492GlnGln: 1.492 ± 0.438
1.612GlnArg: 1.612 ± 0.208
2.089GlnSer: 2.089 ± 0.261
2.03GlnThr: 2.03 ± 0.418
1.701GlnVal: 1.701 ± 0.192
0.418GlnTrp: 0.418 ± 0.13
1.552GlnTyr: 1.552 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
1.552ArgAla: 1.552 ± 0.199
0.328ArgCys: 0.328 ± 0.16
2.328ArgAsp: 2.328 ± 0.235
3.253ArgGlu: 3.253 ± 0.386
1.671ArgPhe: 1.671 ± 0.216
2.03ArgGly: 2.03 ± 0.255
0.686ArgHis: 0.686 ± 0.137
2.656ArgIle: 2.656 ± 0.293
3.014ArgLys: 3.014 ± 0.39
2.0ArgLeu: 2.0 ± 0.255
0.806ArgMet: 0.806 ± 0.144
2.328ArgAsn: 2.328 ± 0.286
0.776ArgPro: 0.776 ± 0.161
1.224ArgGln: 1.224 ± 0.163
1.194ArgArg: 1.194 ± 0.197
1.821ArgSer: 1.821 ± 0.271
2.03ArgThr: 2.03 ± 0.198
2.507ArgVal: 2.507 ± 0.304
0.567ArgTrp: 0.567 ± 0.153
2.238ArgTyr: 2.238 ± 0.227
0.0ArgXaa: 0.0 ± 0.0
Ser
3.641SerAla: 3.641 ± 0.498
0.269SerCys: 0.269 ± 0.086
4.775SerAsp: 4.775 ± 0.388
5.253SerGlu: 5.253 ± 0.368
4.029SerPhe: 4.029 ± 0.397
4.208SerGly: 4.208 ± 0.57
0.925SerHis: 0.925 ± 0.197
5.372SerIle: 5.372 ± 0.58
6.148SerLys: 6.148 ± 0.416
4.895SerLeu: 4.895 ± 0.581
1.552SerMet: 1.552 ± 0.191
3.761SerAsn: 3.761 ± 0.384
1.522SerPro: 1.522 ± 0.195
2.209SerGln: 2.209 ± 0.22
2.597SerArg: 2.597 ± 0.234
5.223SerSer: 5.223 ± 0.793
3.94SerThr: 3.94 ± 0.412
4.029SerVal: 4.029 ± 0.408
0.746SerTrp: 0.746 ± 0.169
2.925SerTyr: 2.925 ± 0.38
0.0SerXaa: 0.0 ± 0.0
Thr
3.223ThrAla: 3.223 ± 0.559
0.269ThrCys: 0.269 ± 0.117
4.059ThrAsp: 4.059 ± 0.37
3.641ThrGlu: 3.641 ± 0.464
2.686ThrPhe: 2.686 ± 0.292
3.522ThrGly: 3.522 ± 0.419
1.015ThrHis: 1.015 ± 0.222
4.596ThrIle: 4.596 ± 0.465
5.462ThrLys: 5.462 ± 0.468
5.223ThrLeu: 5.223 ± 0.345
1.552ThrMet: 1.552 ± 0.236
3.432ThrAsn: 3.432 ± 0.336
1.761ThrPro: 1.761 ± 0.219
1.88ThrGln: 1.88 ± 0.258
2.089ThrArg: 2.089 ± 0.216
4.387ThrSer: 4.387 ± 0.446
3.492ThrThr: 3.492 ± 0.34
4.746ThrVal: 4.746 ± 0.38
0.627ThrTrp: 0.627 ± 0.134
2.865ThrTyr: 2.865 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
3.641ValAla: 3.641 ± 0.355
0.537ValCys: 0.537 ± 0.142
4.626ValAsp: 4.626 ± 0.35
4.656ValGlu: 4.656 ± 0.404
2.746ValPhe: 2.746 ± 0.279
3.373ValGly: 3.373 ± 0.347
1.134ValHis: 1.134 ± 0.24
4.686ValIle: 4.686 ± 0.515
5.73ValLys: 5.73 ± 0.39
4.537ValLeu: 4.537 ± 0.379
1.373ValMet: 1.373 ± 0.243
3.343ValAsn: 3.343 ± 0.392
1.582ValPro: 1.582 ± 0.249
2.268ValGln: 2.268 ± 0.256
2.03ValArg: 2.03 ± 0.278
3.97ValSer: 3.97 ± 0.327
4.387ValThr: 4.387 ± 0.378
4.328ValVal: 4.328 ± 0.381
0.657ValTrp: 0.657 ± 0.167
2.985ValTyr: 2.985 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
0.448TrpAla: 0.448 ± 0.115
0.149TrpCys: 0.149 ± 0.068
0.686TrpAsp: 0.686 ± 0.139
0.806TrpGlu: 0.806 ± 0.173
0.418TrpPhe: 0.418 ± 0.122
0.686TrpGly: 0.686 ± 0.17
0.119TrpHis: 0.119 ± 0.053
0.657TrpIle: 0.657 ± 0.186
0.657TrpLys: 0.657 ± 0.15
0.985TrpLeu: 0.985 ± 0.17
0.478TrpMet: 0.478 ± 0.119
1.045TrpAsn: 1.045 ± 0.214
0.03TrpPro: 0.03 ± 0.026
0.358TrpGln: 0.358 ± 0.095
0.358TrpArg: 0.358 ± 0.101
0.507TrpSer: 0.507 ± 0.111
0.836TrpThr: 0.836 ± 0.158
0.597TrpVal: 0.597 ± 0.169
0.09TrpTrp: 0.09 ± 0.057
0.358TrpTyr: 0.358 ± 0.091
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.209TyrAla: 2.209 ± 0.269
0.239TyrCys: 0.239 ± 0.084
3.164TyrAsp: 3.164 ± 0.333
3.552TyrGlu: 3.552 ± 0.404
1.671TyrPhe: 1.671 ± 0.287
3.074TyrGly: 3.074 ± 0.314
0.686TyrHis: 0.686 ± 0.157
3.641TyrIle: 3.641 ± 0.426
3.97TyrLys: 3.97 ± 0.366
3.671TyrLeu: 3.671 ± 0.421
0.895TyrMet: 0.895 ± 0.15
2.388TyrAsn: 2.388 ± 0.297
1.492TyrPro: 1.492 ± 0.266
1.821TyrGln: 1.821 ± 0.217
1.91TyrArg: 1.91 ± 0.235
3.164TyrSer: 3.164 ± 0.292
3.582TyrThr: 3.582 ± 0.395
2.746TyrVal: 2.746 ± 0.334
0.298TyrTrp: 0.298 ± 0.109
1.97TyrTyr: 1.97 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 154 proteins (33506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski