Duck coronavirus
Average proteome isoelectric point is 7.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F6WGG4|A0A0F6WGG4_9GAMC Nucleoprotein OS=Duck coronavirus OX=300188 GN=N PE=4 SV=1
MM1 pKa = 7.57 LNFEE5 pKa = 4.73 QIIEE9 pKa = 4.22 TGDD12 pKa = 3.65 LVLQQISFNLQHH24 pKa = 6.64 ISSVIEE30 pKa = 3.84 TQIFDD35 pKa = 4.07 PFEE38 pKa = 4.1 CCYY41 pKa = 10.3 YY42 pKa = 10.82 SSGSFYY48 pKa = 10.54 EE49 pKa = 4.4 IEE51 pKa = 4.32 SADD54 pKa = 3.78 DD55 pKa = 3.88 CSDD58 pKa = 4.72 DD59 pKa = 4.13 EE60 pKa = 4.41 FTEE63 pKa = 4.24
Molecular weight: 7.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.321
IPC2_protein 3.3
IPC_protein 3.134
Toseland 3.007
ProMoST 3.35
Dawson 3.172
Bjellqvist 3.452
Wikipedia 3.147
Rodwell 3.032
Grimsley 2.931
Solomon 3.058
Lehninger 3.007
Nozaki 3.35
DTASelect 3.414
Thurlkill 3.096
EMBOSS 3.16
Sillero 3.287
Patrickios 0.006
IPC_peptide 3.045
IPC2_peptide 3.236
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A0F6YSL8|A0A0F6YSL8_9GAMC Envelope small membrane protein OS=Duck coronavirus OX=300188 GN=3c PE=3 SV=1
MM1 pKa = 7.49 KK2 pKa = 9.59 WLSSLGRR9 pKa = 11.84 AFISSYY15 pKa = 11.04 KK16 pKa = 10.43 SLLLFEE22 pKa = 5.71 LRR24 pKa = 11.84 VLDD27 pKa = 5.35 KK28 pKa = 11.1 FILNYY33 pKa = 10.74 GPTCFLISCKK43 pKa = 10.12 RR44 pKa = 11.84 FLLFQVNILYY54 pKa = 10.54 RR55 pKa = 11.84 LVFTPTNSLVV65 pKa = 3.19
Molecular weight: 7.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.228
IPC2_protein 9.443
IPC_protein 9.575
Toseland 9.955
ProMoST 9.706
Dawson 10.189
Bjellqvist 9.926
Wikipedia 10.379
Rodwell 10.54
Grimsley 10.277
Solomon 10.248
Lehninger 10.218
Nozaki 10.043
DTASelect 9.897
Thurlkill 10.028
EMBOSS 10.365
Sillero 10.131
Patrickios 10.292
IPC_peptide 10.248
IPC2_peptide 8.946
IPC2.peptide.svr19 8.256
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
13017
57
6672
1084.8
121.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.853 ± 0.444
3.127 ± 0.158
5.508 ± 0.485
4.602 ± 0.289
5.685 ± 0.341
6.184 ± 0.344
1.874 ± 0.226
4.364 ± 0.488
6.323 ± 0.658
8.896 ± 0.686
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.867 ± 0.148
5.101 ± 0.518
4.064 ± 0.255
3.357 ± 0.377
3.396 ± 0.43
6.922 ± 0.627
5.631 ± 0.426
10.041 ± 0.835
1.444 ± 0.158
4.763 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here