Hubei narna-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIC4|A0A1L3KIC4_9VIRU RNA-directed RNA polymerase OS=Hubei narna-like virus 18 OX=1922948 PE=4 SV=1
MM1 pKa = 6.32QWNWLRR7 pKa = 11.84MICGIISGIVEE18 pKa = 4.39KK19 pKa = 9.65HH20 pKa = 4.97TLPTITGKK28 pKa = 9.64IFRR31 pKa = 11.84GIRR34 pKa = 11.84VKK36 pKa = 10.81HH37 pKa = 5.46VDD39 pKa = 4.5RR40 pKa = 11.84FSLWKK45 pKa = 8.74ICNMLPIVLKK55 pKa = 10.72KK56 pKa = 9.5IARR59 pKa = 11.84ISNVEE64 pKa = 4.01LPHH67 pKa = 6.12SQLEE71 pKa = 4.27VMRR74 pKa = 11.84LVRR77 pKa = 11.84GCITNTRR84 pKa = 11.84SMLEE88 pKa = 3.51IRR90 pKa = 11.84VQLHH94 pKa = 4.98RR95 pKa = 11.84TPFIRR100 pKa = 11.84DD101 pKa = 3.2SAPAQFHH108 pKa = 6.1RR109 pKa = 11.84ACYY112 pKa = 9.07WDD114 pKa = 4.54AEE116 pKa = 4.28ATGYY120 pKa = 9.45PKK122 pKa = 10.62YY123 pKa = 10.74VSNRR127 pKa = 11.84TTDD130 pKa = 2.86RR131 pKa = 11.84TVRR134 pKa = 11.84HH135 pKa = 5.93LALPRR140 pKa = 11.84WVIRR144 pKa = 11.84RR145 pKa = 11.84PLGVPSAHH153 pKa = 6.45LPEE156 pKa = 4.84SATLIVQMDD165 pKa = 3.89EE166 pKa = 4.18TLKK169 pKa = 11.21GDD171 pKa = 4.43IIQIGYY177 pKa = 9.28RR178 pKa = 11.84RR179 pKa = 11.84NDD181 pKa = 4.02LLICDD186 pKa = 4.08YY187 pKa = 11.09KK188 pKa = 11.15VIEE191 pKa = 4.68SGSCQGVSFLGLPGNLHH208 pKa = 5.39GLFRR212 pKa = 11.84EE213 pKa = 4.77GTTVFGDD220 pKa = 3.2NRR222 pKa = 11.84FITVYY227 pKa = 10.76LKK229 pKa = 10.23TNLFHH234 pKa = 7.81DD235 pKa = 4.52SKK237 pKa = 10.58VLPWRR242 pKa = 11.84AEE244 pKa = 3.8HH245 pKa = 5.77TEE247 pKa = 4.08EE248 pKa = 5.19VISSDD253 pKa = 4.22DD254 pKa = 3.13IRR256 pKa = 11.84ATFVSRR262 pKa = 11.84LMDD265 pKa = 3.37TEE267 pKa = 4.89LVQKK271 pKa = 10.36RR272 pKa = 11.84EE273 pKa = 4.13CEE275 pKa = 3.83EE276 pKa = 4.09TQRR279 pKa = 11.84GSHH282 pKa = 6.55LNTATRR288 pKa = 11.84GHH290 pKa = 6.32GNSVRR295 pKa = 11.84IRR297 pKa = 11.84HH298 pKa = 5.88PNGCKK303 pKa = 9.98HH304 pKa = 5.85PFKK307 pKa = 11.14VVFNPFRR314 pKa = 11.84EE315 pKa = 4.41VKK317 pKa = 10.22RR318 pKa = 11.84IEE320 pKa = 4.21NSSPEE325 pKa = 4.29CLCVLVGYY333 pKa = 7.08VVRR336 pKa = 11.84RR337 pKa = 11.84VGQIRR342 pKa = 11.84RR343 pKa = 11.84YY344 pKa = 9.1DD345 pKa = 3.62CTVSVQRR352 pKa = 11.84TADD355 pKa = 3.93HH356 pKa = 6.28ILEE359 pKa = 4.3LLRR362 pKa = 11.84AYY364 pKa = 9.75ILIEE368 pKa = 3.27VHH370 pKa = 5.29IRR372 pKa = 11.84VRR374 pKa = 11.84LEE376 pKa = 3.53YY377 pKa = 10.64GPQLSADD384 pKa = 4.2DD385 pKa = 3.69ITRR388 pKa = 11.84KK389 pKa = 9.42EE390 pKa = 3.97SCGWEE395 pKa = 3.75LAEE398 pKa = 4.13YY399 pKa = 9.43TGLKK403 pKa = 9.72PGFRR407 pKa = 11.84NGEE410 pKa = 4.12NVHH413 pKa = 6.39VISGDD418 pKa = 3.6LFVSIDD424 pKa = 3.39SSFDD428 pKa = 3.43DD429 pKa = 4.87SIVKK433 pKa = 9.72VLLMTIKK440 pKa = 10.64VLAQYY445 pKa = 11.01DD446 pKa = 3.51GTLRR450 pKa = 11.84HH451 pKa = 5.79NSVGIVHH458 pKa = 6.92KK459 pKa = 10.6VSQSTTRR466 pKa = 11.84ASRR469 pKa = 11.84NISASRR475 pKa = 11.84SFRR478 pKa = 11.84WMEE481 pKa = 3.67GHH483 pKa = 7.6PLTQAVFTLVNIITSVAINLVCQTNIQLWSAGHH516 pKa = 6.02NAPVFPEE523 pKa = 3.48RR524 pKa = 11.84LVYY527 pKa = 10.93NKK529 pKa = 9.31FVPNRR534 pKa = 11.84EE535 pKa = 4.06HH536 pKa = 6.67SCYY539 pKa = 10.53ACKK542 pKa = 10.55LCTRR546 pKa = 11.84LCMTAEE552 pKa = 3.85AAKK555 pKa = 9.92PVSKK559 pKa = 10.49CSWYY563 pKa = 8.69IPRR566 pKa = 11.84VIRR569 pKa = 11.84LLSSYY574 pKa = 10.8RR575 pKa = 11.84PDD577 pKa = 3.16TVVEE581 pKa = 4.2GQRR584 pKa = 11.84FNLLADD590 pKa = 3.55ILLTTGTTTGSNKK603 pKa = 9.59PPRR606 pKa = 11.84KK607 pKa = 9.38HH608 pKa = 6.72LSGDD612 pKa = 3.83QIRR615 pKa = 11.84EE616 pKa = 3.74WSPVYY621 pKa = 10.63GFYY624 pKa = 10.7NDD626 pKa = 3.41SHH628 pKa = 6.23TLPDD632 pKa = 3.64VGDD635 pKa = 3.85KK636 pKa = 11.14LSLSFIRR643 pKa = 11.84QGRR646 pKa = 11.84HH647 pKa = 3.31WQLVPVTCPDD657 pKa = 3.0NTGDD661 pKa = 4.14DD662 pKa = 3.92RR663 pKa = 11.84PRR665 pKa = 11.84QDD667 pKa = 2.96ILRR670 pKa = 4.27

Molecular weight: 76.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIC4|A0A1L3KIC4_9VIRU RNA-directed RNA polymerase OS=Hubei narna-like virus 18 OX=1922948 PE=4 SV=1
MM1 pKa = 7.41AVVIEE6 pKa = 4.83AIHH9 pKa = 6.24RR10 pKa = 11.84RR11 pKa = 11.84PFTNLVPAKK20 pKa = 9.63MFAGWLIRR28 pKa = 11.84ASRR31 pKa = 11.84GPGGQKK37 pKa = 9.71YY38 pKa = 8.93VCQQIKK44 pKa = 10.52SLAFDD49 pKa = 3.5YY50 pKa = 10.63RR51 pKa = 11.84VWSITAKK58 pKa = 10.1KK59 pKa = 9.31PNYY62 pKa = 10.22SGDD65 pKa = 3.13IPRR68 pKa = 11.84AFRR71 pKa = 11.84NWLSSLCCHH80 pKa = 6.91AEE82 pKa = 3.74TRR84 pKa = 11.84AQFARR89 pKa = 11.84VARR92 pKa = 11.84MLPVGNKK99 pKa = 9.74LVIDD103 pKa = 3.62QALRR107 pKa = 11.84KK108 pKa = 8.88HH109 pKa = 6.2RR110 pKa = 11.84SVVTRR115 pKa = 11.84RR116 pKa = 11.84PKK118 pKa = 10.67LDD120 pKa = 3.1IGLAHH125 pKa = 6.76QIYY128 pKa = 10.07GYY130 pKa = 10.94ARR132 pKa = 11.84DD133 pKa = 3.93YY134 pKa = 10.31IHH136 pKa = 6.78EE137 pKa = 4.61RR138 pKa = 11.84KK139 pKa = 9.66HH140 pKa = 5.74SLSEE144 pKa = 3.82RR145 pKa = 11.84VSFHH149 pKa = 5.65PTEE152 pKa = 4.49GTASRR157 pKa = 11.84DD158 pKa = 3.55VPASAGGRR166 pKa = 11.84LRR168 pKa = 11.84DD169 pKa = 3.97LVDD172 pKa = 3.27NAHH175 pKa = 6.6RR176 pKa = 11.84VMSQRR181 pKa = 11.84PIVLSEE187 pKa = 3.49YY188 pKa = 10.44LYY190 pKa = 10.35SHH192 pKa = 7.15KK193 pKa = 10.7KK194 pKa = 10.01YY195 pKa = 10.58FDD197 pKa = 3.8DD198 pKa = 6.17AIVEE202 pKa = 4.08AAIYY206 pKa = 9.7TDD208 pKa = 3.96EE209 pKa = 4.79KK210 pKa = 10.69VSTYY214 pKa = 10.92NVDD217 pKa = 3.08VLAIPEE223 pKa = 4.49PGFKK227 pKa = 10.65ARR229 pKa = 11.84VLCKK233 pKa = 10.42FPATALLAGDD243 pKa = 4.33IIRR246 pKa = 11.84RR247 pKa = 11.84QLWPIFEE254 pKa = 5.21SDD256 pKa = 3.22PNMDD260 pKa = 3.65FDD262 pKa = 4.43QDD264 pKa = 3.13IRR266 pKa = 11.84SEE268 pKa = 3.99KK269 pKa = 8.33FQNVIRR275 pKa = 11.84RR276 pKa = 11.84SLNRR280 pKa = 11.84DD281 pKa = 3.06GTIVSSDD288 pKa = 3.23LSNATDD294 pKa = 4.32YY295 pKa = 11.13IPHH298 pKa = 7.19EE299 pKa = 4.17YY300 pKa = 10.76AKK302 pKa = 10.74ALWAGILDD310 pKa = 3.99AFDD313 pKa = 3.81FPEE316 pKa = 3.9WVEE319 pKa = 4.11NYY321 pKa = 9.73LEE323 pKa = 4.93RR324 pKa = 11.84MFAPIRR330 pKa = 11.84MSYY333 pKa = 9.88PDD335 pKa = 3.82GVTVTSCRR343 pKa = 11.84GIQMGTPLSFLTLSLLHH360 pKa = 6.38KK361 pKa = 10.41FCVHH365 pKa = 6.4KK366 pKa = 10.61SGHH369 pKa = 5.4EE370 pKa = 3.66RR371 pKa = 11.84SPYY374 pKa = 9.85IIRR377 pKa = 11.84GDD379 pKa = 3.66DD380 pKa = 3.47LLGVFSSPRR389 pKa = 11.84QYY391 pKa = 9.97LTVMEE396 pKa = 4.84EE397 pKa = 3.47IGFKK401 pKa = 10.28INRR404 pKa = 11.84DD405 pKa = 3.03KK406 pKa = 11.31TVISKK411 pKa = 10.82DD412 pKa = 2.81GGTFAEE418 pKa = 4.4QTVKK422 pKa = 9.39VTWKK426 pKa = 10.53AKK428 pKa = 10.13EE429 pKa = 3.92RR430 pKa = 11.84DD431 pKa = 3.45PLARR435 pKa = 11.84PTLYY439 pKa = 10.96DD440 pKa = 5.51FIVTDD445 pKa = 3.57QKK447 pKa = 11.34VVSSITNLDD456 pKa = 4.41DD457 pKa = 4.2IPFKK461 pKa = 11.22GLIHH465 pKa = 7.18LDD467 pKa = 3.45NKK469 pKa = 10.39GGRR472 pKa = 11.84LRR474 pKa = 11.84QVGRR478 pKa = 11.84WYY480 pKa = 10.51AQWSPYY486 pKa = 9.43YY487 pKa = 9.36PPRR490 pKa = 11.84KK491 pKa = 9.83GKK493 pKa = 9.16VAYY496 pKa = 9.76RR497 pKa = 11.84AIRR500 pKa = 11.84RR501 pKa = 11.84TIGNVLRR508 pKa = 11.84IARR511 pKa = 11.84SLRR514 pKa = 11.84IPITCPMEE522 pKa = 4.79LGGCGIPNKK531 pKa = 10.05RR532 pKa = 11.84GTMQLDD538 pKa = 3.3ANFQHH543 pKa = 7.26RR544 pKa = 11.84SRR546 pKa = 11.84VGYY549 pKa = 9.28AASHH553 pKa = 6.28EE554 pKa = 4.42SHH556 pKa = 6.59NFQLAVRR563 pKa = 11.84KK564 pKa = 9.8LDD566 pKa = 3.46IGNASDD572 pKa = 4.23LLEE575 pKa = 5.36DD576 pKa = 4.03YY577 pKa = 9.75RR578 pKa = 11.84QHH580 pKa = 6.83VADD583 pKa = 4.37LPKK586 pKa = 10.72GKK588 pKa = 9.53SVYY591 pKa = 10.15MFDD594 pKa = 4.45PYY596 pKa = 11.07SSEE599 pKa = 4.32DD600 pKa = 3.36FSGYY604 pKa = 8.49RR605 pKa = 11.84RR606 pKa = 11.84KK607 pKa = 10.47RR608 pKa = 11.84MLLNNTGNYY617 pKa = 8.69SAYY620 pKa = 8.24HH621 pKa = 5.55TKK623 pKa = 10.04PVPLHH628 pKa = 5.84RR629 pKa = 11.84WLAVFSRR636 pKa = 11.84CKK638 pKa = 9.8EE639 pKa = 3.7NRR641 pKa = 11.84ARR643 pKa = 11.84WVPSRR648 pKa = 11.84RR649 pKa = 11.84CSGSDD654 pKa = 3.22LRR656 pKa = 11.84RR657 pKa = 11.84LIDD660 pKa = 4.41RR661 pKa = 11.84IKK663 pKa = 9.24MLSPIYY669 pKa = 10.6VPYY672 pKa = 7.48TTPNAQILSIRR683 pKa = 11.84II684 pKa = 3.65

Molecular weight: 78.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1354

670

684

677.0

77.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.761 ± 1.13

2.142 ± 0.496

5.465 ± 0.492

4.21 ± 0.405

3.693 ± 0.186

5.908 ± 0.044

3.323 ± 0.291

6.942 ± 0.158

4.727 ± 0.498

8.715 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.2

3.84 ± 0.242

5.244 ± 0.334

3.176 ± 0.077

9.38 ± 0.197

7.238 ± 0.053

5.539 ± 0.841

7.607 ± 0.75

1.699 ± 0.066

3.619 ± 0.666
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski