Changjiang tombus-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG78|A0A1L3KG78_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 2 OX=1922813 PE=4 SV=1
MM1 pKa = 7.06GQHH4 pKa = 6.54IVDD7 pKa = 4.95DD8 pKa = 4.15NLNTHH13 pKa = 6.12NNSVANLLRR22 pKa = 11.84GVGEE26 pKa = 3.95RR27 pKa = 11.84VLYY30 pKa = 9.82TDD32 pKa = 5.22RR33 pKa = 11.84NLTKK37 pKa = 10.4CAQPLSGVFEE47 pKa = 4.47GRR49 pKa = 11.84LASYY53 pKa = 9.86KK54 pKa = 10.07RR55 pKa = 11.84RR56 pKa = 11.84IVRR59 pKa = 11.84SVGRR63 pKa = 11.84QSPVTRR69 pKa = 11.84DD70 pKa = 3.31QFVEE74 pKa = 5.2FYY76 pKa = 10.17KK77 pKa = 10.69GPRR80 pKa = 11.84RR81 pKa = 11.84ATYY84 pKa = 9.07EE85 pKa = 3.78RR86 pKa = 11.84AVASLVLKK94 pKa = 7.98PICSRR99 pKa = 11.84DD100 pKa = 3.29ARR102 pKa = 11.84LKK104 pKa = 9.98TFVKK108 pKa = 10.45AEE110 pKa = 4.41KK111 pKa = 10.46INFTLKK117 pKa = 10.21EE118 pKa = 4.11DD119 pKa = 3.76PAPRR123 pKa = 11.84VIQPRR128 pKa = 11.84EE129 pKa = 3.41PRR131 pKa = 11.84FNVEE135 pKa = 3.33VGKK138 pKa = 9.05YY139 pKa = 9.43LRR141 pKa = 11.84PIEE144 pKa = 4.45HH145 pKa = 6.8KK146 pKa = 10.92VYY148 pKa = 10.89DD149 pKa = 5.2AIDD152 pKa = 4.07DD153 pKa = 4.32LFGSPTVMSKK163 pKa = 10.99YY164 pKa = 10.66NSVQTANIIHH174 pKa = 7.2DD175 pKa = 4.04KK176 pKa = 10.14FSSIFGCAVVGLDD189 pKa = 4.03ASRR192 pKa = 11.84FDD194 pKa = 3.47QHH196 pKa = 8.35VSEE199 pKa = 4.21QALKK203 pKa = 10.24FEE205 pKa = 4.33HH206 pKa = 6.77SIYY209 pKa = 10.91DD210 pKa = 3.85GIFNSGEE217 pKa = 3.9LRR219 pKa = 11.84WLLKK223 pKa = 10.46HH224 pKa = 5.83QLHH227 pKa = 5.88NHH229 pKa = 5.8GFAKK233 pKa = 10.86GNDD236 pKa = 2.99GWFKK240 pKa = 9.53YY241 pKa = 8.82QKK243 pKa = 9.88KK244 pKa = 8.61GSRR247 pKa = 11.84MSGDD251 pKa = 3.39MNTSLGNKK259 pKa = 9.73LLMCMMCKK267 pKa = 10.2SYY269 pKa = 11.11LDD271 pKa = 3.72SLKK274 pKa = 10.4IPYY277 pKa = 10.02EE278 pKa = 4.01FVNNGDD284 pKa = 3.6DD285 pKa = 3.64CLVFISRR292 pKa = 11.84KK293 pKa = 9.32HH294 pKa = 5.18LPKK297 pKa = 10.71LEE299 pKa = 4.15TLDD302 pKa = 4.61QYY304 pKa = 11.7FKK306 pKa = 11.52DD307 pKa = 4.17FGFKK311 pKa = 9.22MKK313 pKa = 10.59CEE315 pKa = 3.95PPVFEE320 pKa = 4.24VEE322 pKa = 4.18QIEE325 pKa = 4.59FCQCKK330 pKa = 9.23PVLCNGIWRR339 pKa = 11.84MTRR342 pKa = 11.84NIRR345 pKa = 11.84TALAKK350 pKa = 10.61DD351 pKa = 3.74CTSVNLGHH359 pKa = 7.46DD360 pKa = 3.27IEE362 pKa = 4.93LFRR365 pKa = 11.84RR366 pKa = 11.84WLHH369 pKa = 6.66DD370 pKa = 3.37VSACGAAFSADD381 pKa = 3.75LPVLGSFYY389 pKa = 11.18RR390 pKa = 11.84MLGRR394 pKa = 11.84FGIAGEE400 pKa = 4.32YY401 pKa = 9.6EE402 pKa = 4.11GHH404 pKa = 6.35KK405 pKa = 10.63SEE407 pKa = 4.28FAAYY411 pKa = 9.59RR412 pKa = 11.84SMSRR416 pKa = 11.84GVHH419 pKa = 5.33IPYY422 pKa = 9.69NAPNAQGRR430 pKa = 11.84YY431 pKa = 9.19SFWLSTGINPEE442 pKa = 3.68QQEE445 pKa = 4.07IIEE448 pKa = 4.7NYY450 pKa = 9.79FDD452 pKa = 3.5TAVWGGDD459 pKa = 2.64KK460 pKa = 10.59RR461 pKa = 11.84QIINSIDD468 pKa = 3.45YY469 pKa = 10.57IITHH473 pKa = 6.01GRR475 pKa = 11.84NN476 pKa = 3.04

Molecular weight: 54.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG78|A0A1L3KG78_9VIRU RNA-directed RNA polymerase OS=Changjiang tombus-like virus 2 OX=1922813 PE=4 SV=1
MM1 pKa = 7.79AGTKK5 pKa = 8.78RR6 pKa = 11.84TQRR9 pKa = 11.84TARR12 pKa = 11.84EE13 pKa = 4.02TVDD16 pKa = 3.09QHH18 pKa = 5.51SQRR21 pKa = 11.84SPRR24 pKa = 11.84VNLRR28 pKa = 11.84ARR30 pKa = 11.84LNEE33 pKa = 3.79NVSYY37 pKa = 10.97ISGNEE42 pKa = 3.9YY43 pKa = 8.79NTGAIVPSGSSLGYY57 pKa = 10.39GSVSLAPGNLAGRR70 pKa = 11.84ANAAINGVGRR80 pKa = 11.84FFQKK84 pKa = 10.72GLYY87 pKa = 10.32LPGTFVRR94 pKa = 11.84YY95 pKa = 9.35IPSVGLNTPGNIIIAWLDD113 pKa = 3.49NPDD116 pKa = 5.03MIRR119 pKa = 11.84AWNLLSAGAHH129 pKa = 5.98LNFIRR134 pKa = 11.84DD135 pKa = 3.37VSNAKK140 pKa = 9.06TGPVWQEE147 pKa = 3.3LTVPLTQPPRR157 pKa = 11.84RR158 pKa = 11.84KK159 pKa = 8.3TFMVDD164 pKa = 3.01PQLNFQSNTEE174 pKa = 3.87VDD176 pKa = 4.62LSCQGLFIFCVFGTDD191 pKa = 4.53ISLADD196 pKa = 4.12DD197 pKa = 3.44KK198 pKa = 11.45TYY200 pKa = 11.01GQLLVHH206 pKa = 6.51CKK208 pKa = 8.87MRR210 pKa = 11.84FEE212 pKa = 4.24EE213 pKa = 4.43VKK215 pKa = 11.01SFVTPQQ221 pKa = 2.73

Molecular weight: 24.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

697

221

476

348.5

39.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.026 ± 0.364

2.152 ± 0.38

5.165 ± 0.522

4.591 ± 0.68

5.595 ± 0.295

7.747 ± 0.406

2.582 ± 0.586

5.595 ± 0.295

5.308 ± 1.023

8.178 ± 0.417

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.164

6.026 ± 0.58

4.591 ± 0.617

4.017 ± 0.459

7.03 ± 0.116

6.887 ± 0.169

4.591 ± 1.05

6.887 ± 0.385

1.291 ± 0.032

3.587 ± 0.417
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski