Gallionella capsiferriformans (strain ES-2) (Gallionella ferruginea capsiferriformans (strain ES-2))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Gallionellaceae; Gallionella; Gallionella capsiferriformans

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9SJS4|D9SJS4_GALCS Site-specific DNA-methyltransferase (adenine-specific) OS=Gallionella capsiferriformans (strain ES-2) OX=395494 GN=Galf_0379 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 3.91ATTAVTEE9 pKa = 4.31SQDD12 pKa = 3.88LLVFTDD18 pKa = 3.4NAAAKK23 pKa = 9.25VKK25 pKa = 10.37QLIEE29 pKa = 4.06EE30 pKa = 4.36EE31 pKa = 4.67GNSEE35 pKa = 3.75LKK37 pKa = 10.89LRR39 pKa = 11.84VFVSGGGCSGFQYY52 pKa = 10.94GFTFDD57 pKa = 4.81EE58 pKa = 4.64ITNEE62 pKa = 4.19DD63 pKa = 3.91DD64 pKa = 3.99TVLDD68 pKa = 3.86KK69 pKa = 11.81NGVQLLIDD77 pKa = 3.92PMSYY81 pKa = 10.31QYY83 pKa = 11.58LVGAEE88 pKa = 3.64IDD90 pKa = 3.75YY91 pKa = 10.77TEE93 pKa = 4.43GLEE96 pKa = 4.39GSQFVIKK103 pKa = 10.58NPNATTTCGCGSSFSVV119 pKa = 3.54

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9SHH4|D9SHH4_GALCS Probable chemoreceptor glutamine deamidase CheD OS=Gallionella capsiferriformans (strain ES-2) OX=395494 GN=cheD PE=3 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84LSIIIPILNEE12 pKa = 3.8AVQLPDD18 pKa = 4.63LFAHH22 pKa = 7.1LLPFHH27 pKa = 7.09RR28 pKa = 11.84AGCEE32 pKa = 3.6IIFADD37 pKa = 4.68GGSTDD42 pKa = 3.72GSSKK46 pKa = 10.29LAAVMGFKK54 pKa = 10.22VVQTEE59 pKa = 4.13RR60 pKa = 11.84GRR62 pKa = 11.84ARR64 pKa = 11.84QMNAGAAVAQGNVLLFLHH82 pKa = 7.28ADD84 pKa = 3.09TRR86 pKa = 11.84LPVGAMQQIEE96 pKa = 4.24HH97 pKa = 7.18AFTGPKK103 pKa = 8.86SWGRR107 pKa = 11.84FDD109 pKa = 3.72VCITGRR115 pKa = 11.84PFMLRR120 pKa = 11.84VIGQLMNWRR129 pKa = 11.84SRR131 pKa = 11.84LTGIATGDD139 pKa = 3.1QAIFMRR145 pKa = 11.84RR146 pKa = 11.84IVFEE150 pKa = 4.13RR151 pKa = 11.84LRR153 pKa = 11.84GYY155 pKa = 9.79PDD157 pKa = 3.29QPLMEE162 pKa = 4.94DD163 pKa = 3.25VEE165 pKa = 4.41MCKK168 pKa = 10.16RR169 pKa = 11.84LKK171 pKa = 9.94TFSRR175 pKa = 11.84PACVAHH181 pKa = 6.7CVTTSGRR188 pKa = 11.84RR189 pKa = 11.84WEE191 pKa = 4.09MRR193 pKa = 11.84GVWSTILLMWRR204 pKa = 11.84LRR206 pKa = 11.84WAYY209 pKa = 9.11WRR211 pKa = 11.84GTDD214 pKa = 3.3ATEE217 pKa = 4.09LARR220 pKa = 11.84LYY222 pKa = 10.69RR223 pKa = 4.66

Molecular weight: 25.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2853

0

2853

938798

30

2854

329.1

36.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.368 ± 0.064

1.07 ± 0.016

5.346 ± 0.036

5.954 ± 0.048

3.772 ± 0.028

7.153 ± 0.041

2.398 ± 0.023

5.83 ± 0.034

4.454 ± 0.038

10.902 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.027

3.592 ± 0.032

4.142 ± 0.032

4.267 ± 0.044

5.877 ± 0.038

6.093 ± 0.035

5.07 ± 0.032

7.096 ± 0.041

1.228 ± 0.02

2.658 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski