Aeromonas phage phiAS4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tulanevirus; Aeromonas virus AS4

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1A1S2|E1A1S2_9CAUD E.6 OS=Aeromonas phage phiAS4 OX=879628 GN=phiAS4_ORF0224 PE=4 SV=1
MM1 pKa = 7.38ARR3 pKa = 11.84SNEE6 pKa = 4.06LVEE9 pKa = 5.58KK10 pKa = 10.49AVQLQKK16 pKa = 11.4LLVEE20 pKa = 4.49VEE22 pKa = 4.33ALATEE27 pKa = 4.41HH28 pKa = 7.39DD29 pKa = 4.25YY30 pKa = 11.94GMEE33 pKa = 3.99INGNEE38 pKa = 3.9VDD40 pKa = 4.17FDD42 pKa = 3.99DD43 pKa = 4.78WLSSSCYY50 pKa = 10.46GEE52 pKa = 4.28GSEE55 pKa = 5.24SFGINADD62 pKa = 3.19GSVWMEE68 pKa = 3.87SSCC71 pKa = 5.21

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1A1W6|E1A1W6_9CAUD Uncharacterized protein OS=Aeromonas phage phiAS4 OX=879628 GN=phiAS4_ORF0268 PE=4 SV=1
MM1 pKa = 6.7KK2 pKa = 10.42QNYY5 pKa = 8.83AWLAYY10 pKa = 10.24HH11 pKa = 6.85FGLVPANGCKK21 pKa = 10.18YY22 pKa = 8.02GTRR25 pKa = 11.84GFTFANNNPHH35 pKa = 5.88FAKK38 pKa = 10.6FISTEE43 pKa = 4.03DD44 pKa = 3.04FCAILRR50 pKa = 11.84VARR53 pKa = 11.84HH54 pKa = 6.05CFLKK58 pKa = 10.22GRR60 pKa = 11.84EE61 pKa = 4.27VAVHH65 pKa = 6.03DD66 pKa = 4.72HH67 pKa = 7.04DD68 pKa = 4.44RR69 pKa = 11.84LIYY72 pKa = 10.36GCLSGADD79 pKa = 3.47EE80 pKa = 4.38LFIRR84 pKa = 11.84SRR86 pKa = 11.84DD87 pKa = 3.4TRR89 pKa = 11.84KK90 pKa = 10.46LKK92 pKa = 10.34FQFWLSKK99 pKa = 10.53SATHH103 pKa = 6.37HH104 pKa = 5.09VLNRR108 pKa = 11.84VINTDD113 pKa = 3.85FRR115 pKa = 11.84WIQRR119 pKa = 11.84TKK121 pKa = 10.72RR122 pKa = 11.84GPKK125 pKa = 8.99WISKK129 pKa = 9.96

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

271

0

271

49263

37

1221

181.8

20.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.123 ± 0.21

1.196 ± 0.059

6.256 ± 0.127

6.936 ± 0.193

4.513 ± 0.117

6.281 ± 0.175

1.991 ± 0.079

7.105 ± 0.147

6.778 ± 0.185

7.117 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.083

5.532 ± 0.121

3.575 ± 0.1

3.347 ± 0.106

4.819 ± 0.106

6.492 ± 0.132

5.702 ± 0.23

6.634 ± 0.121

1.478 ± 0.061

4.033 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski