Amino acid dipepetide frequency for Aeromonas phage phiAS4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.481AlaAla: 5.481 ± 0.431
0.65AlaCys: 0.65 ± 0.116
4.182AlaAsp: 4.182 ± 0.264
5.095AlaGlu: 5.095 ± 0.35
2.578AlaPhe: 2.578 ± 0.262
4.689AlaGly: 4.689 ± 0.496
1.502AlaHis: 1.502 ± 0.184
5.278AlaIle: 5.278 ± 0.27
5.603AlaLys: 5.603 ± 0.348
5.42AlaLeu: 5.42 ± 0.346
1.908AlaMet: 1.908 ± 0.2
4.182AlaAsn: 4.182 ± 0.322
1.847AlaPro: 1.847 ± 0.175
2.883AlaGln: 2.883 ± 0.254
3.512AlaArg: 3.512 ± 0.291
4.101AlaSer: 4.101 ± 0.342
3.776AlaThr: 3.776 ± 0.465
4.689AlaVal: 4.689 ± 0.319
1.218AlaTrp: 1.218 ± 0.16
2.639AlaTyr: 2.639 ± 0.206
0.0AlaXaa: 0.0 ± 0.0
Cys
0.893CysAla: 0.893 ± 0.136
0.203CysCys: 0.203 ± 0.071
0.751CysAsp: 0.751 ± 0.138
0.812CysGlu: 0.812 ± 0.127
0.487CysPhe: 0.487 ± 0.093
0.65CysGly: 0.65 ± 0.118
0.284CysHis: 0.284 ± 0.088
0.507CysIle: 0.507 ± 0.1
0.934CysLys: 0.934 ± 0.138
0.69CysLeu: 0.69 ± 0.117
0.365CysMet: 0.365 ± 0.077
0.65CysAsn: 0.65 ± 0.116
0.65CysPro: 0.65 ± 0.111
0.447CysGln: 0.447 ± 0.112
0.731CysArg: 0.731 ± 0.122
0.873CysSer: 0.873 ± 0.134
0.609CysThr: 0.609 ± 0.112
0.67CysVal: 0.67 ± 0.115
0.203CysTrp: 0.203 ± 0.072
0.548CysTyr: 0.548 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
4.019AspAla: 4.019 ± 0.233
0.65AspCys: 0.65 ± 0.099
4.385AspAsp: 4.385 ± 0.358
4.892AspGlu: 4.892 ± 0.394
3.634AspPhe: 3.634 ± 0.248
4.953AspGly: 4.953 ± 0.283
0.913AspHis: 0.913 ± 0.16
5.217AspIle: 5.217 ± 0.269
3.41AspLys: 3.41 ± 0.251
4.161AspLeu: 4.161 ± 0.325
2.213AspMet: 2.213 ± 0.194
2.537AspAsn: 2.537 ± 0.224
2.071AspPro: 2.071 ± 0.214
2.01AspGln: 2.01 ± 0.192
2.659AspArg: 2.659 ± 0.202
3.857AspSer: 3.857 ± 0.289
3.207AspThr: 3.207 ± 0.25
4.06AspVal: 4.06 ± 0.257
1.218AspTrp: 1.218 ± 0.188
2.497AspTyr: 2.497 ± 0.236
0.0AspXaa: 0.0 ± 0.0
Glu
5.055GluAla: 5.055 ± 0.351
0.771GluCys: 0.771 ± 0.129
3.268GluAsp: 3.268 ± 0.284
4.608GluGlu: 4.608 ± 0.359
3.329GluPhe: 3.329 ± 0.249
3.349GluGly: 3.349 ± 0.282
1.462GluHis: 1.462 ± 0.142
6.049GluIle: 6.049 ± 0.344
4.913GluLys: 4.913 ± 0.342
6.415GluLeu: 6.415 ± 0.424
2.7GluMet: 2.7 ± 0.26
3.938GluAsn: 3.938 ± 0.273
1.624GluPro: 1.624 ± 0.19
2.537GluGln: 2.537 ± 0.23
3.126GluArg: 3.126 ± 0.253
3.898GluSer: 3.898 ± 0.298
3.471GluThr: 3.471 ± 0.294
4.486GluVal: 4.486 ± 0.352
1.319GluTrp: 1.319 ± 0.207
3.045GluTyr: 3.045 ± 0.243
0.0GluXaa: 0.0 ± 0.0
Phe
3.41PheAla: 3.41 ± 0.273
0.67PheCys: 0.67 ± 0.125
3.837PheAsp: 3.837 ± 0.268
3.898PheGlu: 3.898 ± 0.317
1.665PhePhe: 1.665 ± 0.189
3.025PheGly: 3.025 ± 0.282
0.71PheHis: 0.71 ± 0.122
3.086PheIle: 3.086 ± 0.254
3.329PheLys: 3.329 ± 0.212
2.537PheLeu: 2.537 ± 0.262
1.157PheMet: 1.157 ± 0.172
2.578PheAsn: 2.578 ± 0.247
1.279PhePro: 1.279 ± 0.147
1.218PheGln: 1.218 ± 0.145
1.807PheArg: 1.807 ± 0.157
3.065PheSer: 3.065 ± 0.243
2.477PheThr: 2.477 ± 0.214
3.41PheVal: 3.41 ± 0.263
0.548PheTrp: 0.548 ± 0.087
1.421PheTyr: 1.421 ± 0.177
0.0PheXaa: 0.0 ± 0.0
Gly
4.019GlyAla: 4.019 ± 0.416
0.812GlyCys: 0.812 ± 0.122
4.101GlyAsp: 4.101 ± 0.311
3.857GlyGlu: 3.857 ± 0.267
2.598GlyPhe: 2.598 ± 0.241
3.796GlyGly: 3.796 ± 0.333
1.238GlyHis: 1.238 ± 0.155
4.567GlyIle: 4.567 ± 0.235
4.689GlyLys: 4.689 ± 0.355
4.385GlyLeu: 4.385 ± 0.297
1.685GlyMet: 1.685 ± 0.194
3.492GlyAsn: 3.492 ± 0.402
1.665GlyPro: 1.665 ± 0.17
2.456GlyGln: 2.456 ± 0.234
2.74GlyArg: 2.74 ± 0.224
4.385GlySer: 4.385 ± 0.345
3.898GlyThr: 3.898 ± 0.388
4.263GlyVal: 4.263 ± 0.299
1.035GlyTrp: 1.035 ± 0.132
3.126GlyTyr: 3.126 ± 0.25
0.0GlyXaa: 0.0 ± 0.0
His
1.015HisAla: 1.015 ± 0.136
0.284HisCys: 0.284 ± 0.07
1.644HisAsp: 1.644 ± 0.207
1.482HisGlu: 1.482 ± 0.173
0.995HisPhe: 0.995 ± 0.165
1.36HisGly: 1.36 ± 0.151
0.467HisHis: 0.467 ± 0.096
1.786HisIle: 1.786 ± 0.199
1.218HisLys: 1.218 ± 0.154
1.847HisLeu: 1.847 ± 0.218
0.426HisMet: 0.426 ± 0.084
0.913HisAsn: 0.913 ± 0.136
0.792HisPro: 0.792 ± 0.134
0.487HisGln: 0.487 ± 0.109
0.934HisArg: 0.934 ± 0.143
1.056HisSer: 1.056 ± 0.146
0.954HisThr: 0.954 ± 0.137
1.218HisVal: 1.218 ± 0.149
0.365HisTrp: 0.365 ± 0.073
0.67HisTyr: 0.67 ± 0.106
0.0HisXaa: 0.0 ± 0.0
Ile
5.704IleAla: 5.704 ± 0.379
1.015IleCys: 1.015 ± 0.136
5.176IleAsp: 5.176 ± 0.305
4.71IleGlu: 4.71 ± 0.318
2.7IlePhe: 2.7 ± 0.204
4.161IleGly: 4.161 ± 0.326
1.198IleHis: 1.198 ± 0.151
4.527IleIle: 4.527 ± 0.294
4.689IleLys: 4.689 ± 0.312
4.304IleLeu: 4.304 ± 0.33
1.888IleMet: 1.888 ± 0.178
4.304IleAsn: 4.304 ± 0.249
2.984IlePro: 2.984 ± 0.237
2.334IleGln: 2.334 ± 0.201
3.613IleArg: 3.613 ± 0.306
4.791IleSer: 4.791 ± 0.345
4.953IleThr: 4.953 ± 0.321
4.689IleVal: 4.689 ± 0.308
0.67IleTrp: 0.67 ± 0.099
2.639IleTyr: 2.639 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
5.4LysAla: 5.4 ± 0.336
0.65LysCys: 0.65 ± 0.101
3.573LysAsp: 3.573 ± 0.274
4.425LysGlu: 4.425 ± 0.32
3.39LysPhe: 3.39 ± 0.317
3.451LysGly: 3.451 ± 0.298
1.665LysHis: 1.665 ± 0.168
4.628LysIle: 4.628 ± 0.279
4.08LysLys: 4.08 ± 0.371
5.116LysLeu: 5.116 ± 0.323
2.578LysMet: 2.578 ± 0.213
4.243LysAsn: 4.243 ± 0.351
2.314LysPro: 2.314 ± 0.248
2.517LysGln: 2.517 ± 0.252
3.289LysArg: 3.289 ± 0.25
4.649LysSer: 4.649 ± 0.322
4.121LysThr: 4.121 ± 0.279
3.735LysVal: 3.735 ± 0.268
0.974LysTrp: 0.974 ± 0.121
2.984LysTyr: 2.984 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
5.359LeuAla: 5.359 ± 0.414
0.934LeuCys: 0.934 ± 0.167
4.446LeuAsp: 4.446 ± 0.312
4.831LeuGlu: 4.831 ± 0.336
3.146LeuPhe: 3.146 ± 0.289
4.588LeuGly: 4.588 ± 0.278
1.218LeuHis: 1.218 ± 0.136
4.344LeuIle: 4.344 ± 0.324
5.197LeuLys: 5.197 ± 0.323
4.669LeuLeu: 4.669 ± 0.308
2.558LeuMet: 2.558 ± 0.206
4.019LeuAsn: 4.019 ± 0.299
3.228LeuPro: 3.228 ± 0.251
2.334LeuGln: 2.334 ± 0.201
3.816LeuArg: 3.816 ± 0.248
4.892LeuSer: 4.892 ± 0.271
3.329LeuThr: 3.329 ± 0.291
4.892LeuVal: 4.892 ± 0.317
0.69LeuTrp: 0.69 ± 0.108
2.68LeuTyr: 2.68 ± 0.252
0.0LeuXaa: 0.0 ± 0.0
Met
2.314MetAla: 2.314 ± 0.207
0.406MetCys: 0.406 ± 0.099
1.319MetAsp: 1.319 ± 0.181
1.786MetGlu: 1.786 ± 0.176
1.746MetPhe: 1.746 ± 0.167
1.644MetGly: 1.644 ± 0.182
0.69MetHis: 0.69 ± 0.119
2.192MetIle: 2.192 ± 0.218
2.801MetLys: 2.801 ± 0.244
2.497MetLeu: 2.497 ± 0.238
0.853MetMet: 0.853 ± 0.13
1.644MetAsn: 1.644 ± 0.166
1.238MetPro: 1.238 ± 0.156
1.198MetGln: 1.198 ± 0.17
1.462MetArg: 1.462 ± 0.175
2.578MetSer: 2.578 ± 0.251
1.644MetThr: 1.644 ± 0.18
1.604MetVal: 1.604 ± 0.136
0.345MetTrp: 0.345 ± 0.094
0.974MetTyr: 0.974 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.796AsnAla: 3.796 ± 0.328
0.467AsnCys: 0.467 ± 0.109
3.167AsnAsp: 3.167 ± 0.261
3.613AsnGlu: 3.613 ± 0.303
2.598AsnPhe: 2.598 ± 0.225
4.364AsnGly: 4.364 ± 0.354
1.056AsnHis: 1.056 ± 0.166
3.837AsnIle: 3.837 ± 0.291
3.39AsnLys: 3.39 ± 0.234
3.593AsnLeu: 3.593 ± 0.272
1.725AsnMet: 1.725 ± 0.194
3.004AsnAsn: 3.004 ± 0.28
2.781AsnPro: 2.781 ± 0.248
1.827AsnGln: 1.827 ± 0.184
2.801AsnArg: 2.801 ± 0.224
3.877AsnSer: 3.877 ± 0.265
3.349AsnThr: 3.349 ± 0.324
3.289AsnVal: 3.289 ± 0.274
0.792AsnTrp: 0.792 ± 0.153
1.989AsnTyr: 1.989 ± 0.217
0.0AsnXaa: 0.0 ± 0.0
Pro
2.558ProAla: 2.558 ± 0.238
0.386ProCys: 0.386 ± 0.095
2.334ProAsp: 2.334 ± 0.258
2.943ProGlu: 2.943 ± 0.251
1.583ProPhe: 1.583 ± 0.169
2.497ProGly: 2.497 ± 0.237
0.771ProHis: 0.771 ± 0.107
2.213ProIle: 2.213 ± 0.196
2.111ProLys: 2.111 ± 0.192
2.091ProLeu: 2.091 ± 0.19
1.157ProMet: 1.157 ± 0.158
2.355ProAsn: 2.355 ± 0.21
0.873ProPro: 0.873 ± 0.135
0.893ProGln: 0.893 ± 0.13
1.502ProArg: 1.502 ± 0.179
2.578ProSer: 2.578 ± 0.266
2.03ProThr: 2.03 ± 0.231
2.416ProVal: 2.416 ± 0.229
0.548ProTrp: 0.548 ± 0.109
1.908ProTyr: 1.908 ± 0.197
0.0ProXaa: 0.0 ± 0.0
Gln
2.598GlnAla: 2.598 ± 0.228
0.325GlnCys: 0.325 ± 0.082
1.441GlnAsp: 1.441 ± 0.197
1.705GlnGlu: 1.705 ± 0.199
1.583GlnPhe: 1.583 ± 0.156
1.482GlnGly: 1.482 ± 0.197
0.832GlnHis: 0.832 ± 0.106
2.68GlnIle: 2.68 ± 0.25
2.395GlnLys: 2.395 ± 0.204
3.045GlnLeu: 3.045 ± 0.218
1.096GlnMet: 1.096 ± 0.163
1.807GlnAsn: 1.807 ± 0.164
0.974GlnPro: 0.974 ± 0.162
1.259GlnGln: 1.259 ± 0.181
1.198GlnArg: 1.198 ± 0.179
2.071GlnSer: 2.071 ± 0.209
2.416GlnThr: 2.416 ± 0.249
2.598GlnVal: 2.598 ± 0.206
0.386GlnTrp: 0.386 ± 0.096
1.583GlnTyr: 1.583 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
3.126ArgAla: 3.126 ± 0.245
0.873ArgCys: 0.873 ± 0.143
3.065ArgAsp: 3.065 ± 0.236
3.248ArgGlu: 3.248 ± 0.255
2.375ArgPhe: 2.375 ± 0.192
2.801ArgGly: 2.801 ± 0.208
1.157ArgHis: 1.157 ± 0.141
3.634ArgIle: 3.634 ± 0.235
3.228ArgLys: 3.228 ± 0.284
3.593ArgLeu: 3.593 ± 0.281
1.543ArgMet: 1.543 ± 0.211
2.517ArgAsn: 2.517 ± 0.225
1.319ArgPro: 1.319 ± 0.151
2.05ArgGln: 2.05 ± 0.264
2.294ArgArg: 2.294 ± 0.217
2.517ArgSer: 2.517 ± 0.218
2.497ArgThr: 2.497 ± 0.233
3.248ArgVal: 3.248 ± 0.237
0.893ArgTrp: 0.893 ± 0.137
2.213ArgTyr: 2.213 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
4.425SerAla: 4.425 ± 0.308
0.812SerCys: 0.812 ± 0.123
4.019SerAsp: 4.019 ± 0.269
4.567SerGlu: 4.567 ± 0.291
2.923SerPhe: 2.923 ± 0.236
4.669SerGly: 4.669 ± 0.407
1.38SerHis: 1.38 ± 0.18
4.913SerIle: 4.913 ± 0.262
3.979SerLys: 3.979 ± 0.255
4.304SerLeu: 4.304 ± 0.285
1.482SerMet: 1.482 ± 0.179
3.248SerAsn: 3.248 ± 0.273
2.395SerPro: 2.395 ± 0.231
1.868SerGln: 1.868 ± 0.161
3.512SerArg: 3.512 ± 0.243
4.547SerSer: 4.547 ± 0.344
3.613SerThr: 3.613 ± 0.347
4.567SerVal: 4.567 ± 0.311
0.913SerTrp: 0.913 ± 0.149
2.781SerTyr: 2.781 ± 0.22
0.0SerXaa: 0.0 ± 0.0
Thr
3.958ThrAla: 3.958 ± 0.453
0.507ThrCys: 0.507 ± 0.106
3.39ThrAsp: 3.39 ± 0.254
3.654ThrGlu: 3.654 ± 0.293
2.558ThrPhe: 2.558 ± 0.219
4.04ThrGly: 4.04 ± 0.378
0.974ThrHis: 0.974 ± 0.124
4.08ThrIle: 4.08 ± 0.28
3.289ThrLys: 3.289 ± 0.213
4.304ThrLeu: 4.304 ± 0.315
1.685ThrMet: 1.685 ± 0.168
3.065ThrAsn: 3.065 ± 0.269
3.086ThrPro: 3.086 ± 0.285
1.807ThrGln: 1.807 ± 0.205
2.801ThrArg: 2.801 ± 0.297
3.877ThrSer: 3.877 ± 0.332
3.086ThrThr: 3.086 ± 0.27
4.161ThrVal: 4.161 ± 0.312
0.873ThrTrp: 0.873 ± 0.145
1.807ThrTyr: 1.807 ± 0.196
0.0ThrXaa: 0.0 ± 0.0
Val
3.958ValAla: 3.958 ± 0.281
0.853ValCys: 0.853 ± 0.167
4.466ValAsp: 4.466 ± 0.284
5.237ValGlu: 5.237 ± 0.338
2.72ValPhe: 2.72 ± 0.275
4.182ValGly: 4.182 ± 0.355
1.177ValHis: 1.177 ± 0.176
4.405ValIle: 4.405 ± 0.341
5.034ValLys: 5.034 ± 0.298
4.466ValLeu: 4.466 ± 0.294
1.989ValMet: 1.989 ± 0.199
3.857ValAsn: 3.857 ± 0.329
2.416ValPro: 2.416 ± 0.257
1.502ValGln: 1.502 ± 0.199
3.37ValArg: 3.37 ± 0.235
4.283ValSer: 4.283 ± 0.32
3.999ValThr: 3.999 ± 0.373
4.608ValVal: 4.608 ± 0.299
0.893ValTrp: 0.893 ± 0.126
2.923ValTyr: 2.923 ± 0.266
0.0ValXaa: 0.0 ± 0.0
Trp
1.076TrpAla: 1.076 ± 0.137
0.284TrpCys: 0.284 ± 0.082
0.954TrpAsp: 0.954 ± 0.114
1.076TrpGlu: 1.076 ± 0.124
0.771TrpPhe: 0.771 ± 0.119
0.629TrpGly: 0.629 ± 0.117
0.264TrpHis: 0.264 ± 0.094
0.873TrpIle: 0.873 ± 0.123
1.137TrpLys: 1.137 ± 0.139
1.177TrpLeu: 1.177 ± 0.166
0.65TrpMet: 0.65 ± 0.1
0.609TrpAsn: 0.609 ± 0.119
0.467TrpPro: 0.467 ± 0.097
0.69TrpGln: 0.69 ± 0.121
0.832TrpArg: 0.832 ± 0.129
0.792TrpSer: 0.792 ± 0.137
0.751TrpThr: 0.751 ± 0.13
0.913TrpVal: 0.913 ± 0.148
0.183TrpTrp: 0.183 ± 0.058
0.65TrpTyr: 0.65 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.065TyrAla: 3.065 ± 0.252
0.406TyrCys: 0.406 ± 0.086
3.045TyrAsp: 3.045 ± 0.215
2.923TyrGlu: 2.923 ± 0.235
1.746TyrPhe: 1.746 ± 0.169
2.477TyrGly: 2.477 ± 0.232
0.954TyrHis: 0.954 ± 0.159
2.233TyrIle: 2.233 ± 0.243
2.355TyrLys: 2.355 ± 0.211
2.558TyrLeu: 2.558 ± 0.206
1.259TyrMet: 1.259 ± 0.127
2.355TyrAsn: 2.355 ± 0.242
1.746TyrPro: 1.746 ± 0.214
1.157TyrGln: 1.157 ± 0.125
2.192TyrArg: 2.192 ± 0.224
2.233TyrSer: 2.233 ± 0.192
2.842TyrThr: 2.842 ± 0.216
2.822TyrVal: 2.822 ± 0.267
0.71TyrTrp: 0.71 ± 0.114
1.259TyrTyr: 1.259 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 271 proteins (49263 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski