Haemophilus pittmaniae HK 85

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Haemophilus; Haemophilus pittmaniae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2390 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9Q7E2|F9Q7E2_9PAST Penicillin-binding protein transpeptidase domain protein OS=Haemophilus pittmaniae HK 85 OX=1035188 GN=HMPREF9952_0186 PE=4 SV=1
MM1 pKa = 7.41TIAYY5 pKa = 8.68SQLNQQLKK13 pKa = 10.03SAGIGINATEE23 pKa = 3.85LHH25 pKa = 6.35GFLSGLLCGGLRR37 pKa = 11.84DD38 pKa = 4.39QSWLPLLYY46 pKa = 10.45QFSNDD51 pKa = 2.68NHH53 pKa = 6.96AYY55 pKa = 9.78PMALTAAVSEE65 pKa = 5.33FYY67 pKa = 10.71QQIADD72 pKa = 3.92EE73 pKa = 4.59LADD76 pKa = 3.38VDD78 pKa = 4.32GFEE81 pKa = 4.72FTPQLVGEE89 pKa = 4.35EE90 pKa = 4.07AGGFARR96 pKa = 11.84ADD98 pKa = 3.79SLSEE102 pKa = 3.78WANHH106 pKa = 5.6FFLGLGLARR115 pKa = 11.84SDD117 pKa = 3.68LAQEE121 pKa = 3.93TGEE124 pKa = 4.04IGEE127 pKa = 5.39AIDD130 pKa = 5.33DD131 pKa = 4.31LQDD134 pKa = 3.25ICQLGYY140 pKa = 10.79DD141 pKa = 4.0ADD143 pKa = 4.3DD144 pKa = 4.52NEE146 pKa = 4.99DD147 pKa = 3.9EE148 pKa = 4.38IEE150 pKa = 4.08EE151 pKa = 4.0ALEE154 pKa = 4.11EE155 pKa = 4.38IIEE158 pKa = 4.08YY159 pKa = 10.67LRR161 pKa = 11.84TIAMLFYY168 pKa = 11.25NHH170 pKa = 6.59FNPPKK175 pKa = 8.91TAEE178 pKa = 4.24KK179 pKa = 10.16PLLHH183 pKa = 6.8

Molecular weight: 20.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9Q724|F9Q724_9PAST Ribonuclease P protein component OS=Haemophilus pittmaniae HK 85 OX=1035188 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.68RR12 pKa = 11.84NRR14 pKa = 11.84THH16 pKa = 7.28GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2390

0

2390

631774

37

2810

264.3

29.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.31 ± 0.12

1.06 ± 0.022

5.063 ± 0.041

6.054 ± 0.067

4.31 ± 0.043

6.904 ± 0.056

2.078 ± 0.025

6.522 ± 0.05

5.604 ± 0.046

10.774 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.458 ± 0.024

4.625 ± 0.05

3.88 ± 0.036

4.965 ± 0.045

4.668 ± 0.045

5.778 ± 0.098

4.971 ± 0.041

6.601 ± 0.041

1.225 ± 0.022

3.148 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski