Amino acid dipepetide frequency for Haemophilus pittmaniae HK 85

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.06AlaAla: 9.06 ± 0.33
1.0AlaCys: 1.0 ± 0.043
5.03AlaAsp: 5.03 ± 0.131
6.586AlaGlu: 6.586 ± 0.127
3.748AlaPhe: 3.748 ± 0.087
6.054AlaGly: 6.054 ± 0.109
1.554AlaHis: 1.554 ± 0.055
6.257AlaIle: 6.257 ± 0.107
5.85AlaLys: 5.85 ± 0.12
10.773AlaLeu: 10.773 ± 0.15
2.677AlaMet: 2.677 ± 0.065
4.043AlaAsn: 4.043 ± 0.155
2.973AlaPro: 2.973 ± 0.074
4.733AlaGln: 4.733 ± 0.12
3.897AlaArg: 3.897 ± 0.077
4.589AlaSer: 4.589 ± 0.387
4.533AlaThr: 4.533 ± 0.163
6.254AlaVal: 6.254 ± 0.092
0.929AlaTrp: 0.929 ± 0.035
2.559AlaTyr: 2.559 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.904CysAla: 0.904 ± 0.038
0.177CysCys: 0.177 ± 0.019
0.619CysAsp: 0.619 ± 0.034
0.573CysGlu: 0.573 ± 0.033
0.473CysPhe: 0.473 ± 0.024
1.097CysGly: 1.097 ± 0.044
0.355CysHis: 0.355 ± 0.028
0.614CysIle: 0.614 ± 0.034
0.407CysLys: 0.407 ± 0.026
1.053CysLeu: 1.053 ± 0.039
0.218CysMet: 0.218 ± 0.018
0.353CysAsn: 0.353 ± 0.024
0.483CysPro: 0.483 ± 0.036
0.486CysGln: 0.486 ± 0.028
0.538CysArg: 0.538 ± 0.029
0.639CysSer: 0.639 ± 0.031
0.434CysThr: 0.434 ± 0.027
0.647CysVal: 0.647 ± 0.034
0.146CysTrp: 0.146 ± 0.016
0.381CysTyr: 0.381 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.188AspAla: 4.188 ± 0.091
0.617AspCys: 0.617 ± 0.035
2.506AspAsp: 2.506 ± 0.07
3.856AspGlu: 3.856 ± 0.086
2.647AspPhe: 2.647 ± 0.07
3.432AspGly: 3.432 ± 0.078
0.836AspHis: 0.836 ± 0.038
3.59AspIle: 3.59 ± 0.075
3.419AspLys: 3.419 ± 0.087
5.007AspLeu: 5.007 ± 0.092
1.151AspMet: 1.151 ± 0.037
2.498AspAsn: 2.498 ± 0.066
2.045AspPro: 2.045 ± 0.062
1.564AspGln: 1.564 ± 0.054
2.026AspArg: 2.026 ± 0.071
2.711AspSer: 2.711 ± 0.127
2.017AspThr: 2.017 ± 0.058
3.495AspVal: 3.495 ± 0.077
0.855AspTrp: 0.855 ± 0.042
2.176AspTyr: 2.176 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
4.75GluAla: 4.75 ± 0.115
0.489GluCys: 0.489 ± 0.027
2.566GluAsp: 2.566 ± 0.059
3.647GluGlu: 3.647 ± 0.107
2.501GluPhe: 2.501 ± 0.067
3.444GluGly: 3.444 ± 0.08
1.394GluHis: 1.394 ± 0.047
4.388GluIle: 4.388 ± 0.086
4.361GluLys: 4.361 ± 0.093
6.506GluLeu: 6.506 ± 0.125
1.63GluMet: 1.63 ± 0.053
3.406GluAsn: 3.406 ± 0.079
1.974GluPro: 1.974 ± 0.059
4.044GluGln: 4.044 ± 0.101
3.352GluArg: 3.352 ± 0.092
2.898GluSer: 2.898 ± 0.066
2.9GluThr: 2.9 ± 0.063
3.94GluVal: 3.94 ± 0.08
0.668GluTrp: 0.668 ± 0.032
1.679GluTyr: 1.679 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
4.114PheAla: 4.114 ± 0.086
0.625PheCys: 0.625 ± 0.035
2.716PheAsp: 2.716 ± 0.072
2.265PheGlu: 2.265 ± 0.063
1.899PhePhe: 1.899 ± 0.065
3.446PheGly: 3.446 ± 0.092
0.818PheHis: 0.818 ± 0.038
2.954PheIle: 2.954 ± 0.07
2.089PheLys: 2.089 ± 0.062
3.864PheLeu: 3.864 ± 0.097
1.1PheMet: 1.1 ± 0.035
2.267PheAsn: 2.267 ± 0.064
1.51PhePro: 1.51 ± 0.055
1.336PheGln: 1.336 ± 0.044
1.556PheArg: 1.556 ± 0.049
3.264PheSer: 3.264 ± 0.081
2.292PheThr: 2.292 ± 0.071
2.938PheVal: 2.938 ± 0.08
0.541PheTrp: 0.541 ± 0.032
1.508PheTyr: 1.508 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
5.736GlyAla: 5.736 ± 0.132
0.858GlyCys: 0.858 ± 0.044
3.354GlyAsp: 3.354 ± 0.078
4.342GlyGlu: 4.342 ± 0.087
3.265GlyPhe: 3.265 ± 0.08
4.961GlyGly: 4.961 ± 0.114
1.464GlyHis: 1.464 ± 0.049
5.048GlyIle: 5.048 ± 0.102
4.821GlyLys: 4.821 ± 0.099
7.395GlyLeu: 7.395 ± 0.126
1.887GlyMet: 1.887 ± 0.065
2.882GlyAsn: 2.882 ± 0.082
1.287GlyPro: 1.287 ± 0.053
2.735GlyGln: 2.735 ± 0.067
3.237GlyArg: 3.237 ± 0.07
3.813GlySer: 3.813 ± 0.088
3.275GlyThr: 3.275 ± 0.08
5.23GlyVal: 5.23 ± 0.099
0.878GlyTrp: 0.878 ± 0.039
2.575GlyTyr: 2.575 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.402HisAla: 1.402 ± 0.048
0.378HisCys: 0.378 ± 0.023
0.844HisAsp: 0.844 ± 0.042
0.896HisGlu: 0.896 ± 0.036
1.222HisPhe: 1.222 ± 0.043
1.415HisGly: 1.415 ± 0.054
0.665HisHis: 0.665 ± 0.036
1.477HisIle: 1.477 ± 0.049
1.019HisLys: 1.019 ± 0.037
2.286HisLeu: 2.286 ± 0.062
0.4HisMet: 0.4 ± 0.027
0.991HisAsn: 0.991 ± 0.039
1.054HisPro: 1.054 ± 0.042
1.181HisGln: 1.181 ± 0.044
1.051HisArg: 1.051 ± 0.043
1.325HisSer: 1.325 ± 0.047
0.907HisThr: 0.907 ± 0.038
0.999HisVal: 0.999 ± 0.035
0.402HisTrp: 0.402 ± 0.028
0.866HisTyr: 0.866 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.89IleAla: 6.89 ± 0.109
0.791IleCys: 0.791 ± 0.033
3.818IleAsp: 3.818 ± 0.087
4.316IleGlu: 4.316 ± 0.089
2.692IlePhe: 2.692 ± 0.08
5.04IleGly: 5.04 ± 0.115
1.216IleHis: 1.216 ± 0.047
3.967IleIle: 3.967 ± 0.082
3.351IleLys: 3.351 ± 0.079
6.077IleLeu: 6.077 ± 0.123
1.35IleMet: 1.35 ± 0.047
3.254IleAsn: 3.254 ± 0.062
2.716IlePro: 2.716 ± 0.079
2.377IleGln: 2.377 ± 0.069
2.952IleArg: 2.952 ± 0.083
4.161IleSer: 4.161 ± 0.09
3.33IleThr: 3.33 ± 0.075
4.256IleVal: 4.256 ± 0.089
0.654IleTrp: 0.654 ± 0.035
2.01IleTyr: 2.01 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
5.448LysAla: 5.448 ± 0.12
0.362LysCys: 0.362 ± 0.025
2.848LysAsp: 2.848 ± 0.069
3.455LysGlu: 3.455 ± 0.089
1.839LysPhe: 1.839 ± 0.051
3.672LysGly: 3.672 ± 0.079
1.136LysHis: 1.136 ± 0.045
3.656LysIle: 3.656 ± 0.079
3.068LysLys: 3.068 ± 0.085
5.629LysLeu: 5.629 ± 0.095
1.746LysMet: 1.746 ± 0.063
2.833LysAsn: 2.833 ± 0.075
2.371LysPro: 2.371 ± 0.065
3.031LysGln: 3.031 ± 0.072
2.746LysArg: 2.746 ± 0.071
3.158LysSer: 3.158 ± 0.079
3.042LysThr: 3.042 ± 0.074
3.875LysVal: 3.875 ± 0.073
0.608LysTrp: 0.608 ± 0.031
1.518LysTyr: 1.518 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
10.874LeuAla: 10.874 ± 0.154
1.241LeuCys: 1.241 ± 0.047
5.616LeuAsp: 5.616 ± 0.093
5.57LeuGlu: 5.57 ± 0.115
4.597LeuPhe: 4.597 ± 0.127
7.495LeuGly: 7.495 ± 0.14
2.066LeuHis: 2.066 ± 0.061
6.589LeuIle: 6.589 ± 0.123
5.529LeuLys: 5.529 ± 0.092
11.783LeuLeu: 11.783 ± 0.193
2.521LeuMet: 2.521 ± 0.071
5.206LeuAsn: 5.206 ± 0.117
4.937LeuPro: 4.937 ± 0.095
4.899LeuGln: 4.899 ± 0.114
5.002LeuArg: 5.002 ± 0.103
7.409LeuSer: 7.409 ± 0.125
5.88LeuThr: 5.88 ± 0.09
6.589LeuVal: 6.589 ± 0.116
1.23LeuTrp: 1.23 ± 0.053
2.708LeuTyr: 2.708 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.547MetAla: 2.547 ± 0.065
0.169MetCys: 0.169 ± 0.014
1.086MetAsp: 1.086 ± 0.041
1.189MetGlu: 1.189 ± 0.046
0.893MetPhe: 0.893 ± 0.036
1.575MetGly: 1.575 ± 0.062
0.438MetHis: 0.438 ± 0.027
1.458MetIle: 1.458 ± 0.053
1.578MetLys: 1.578 ± 0.053
2.806MetLeu: 2.806 ± 0.074
0.717MetMet: 0.717 ± 0.035
1.136MetAsn: 1.136 ± 0.041
1.193MetPro: 1.193 ± 0.046
1.402MetGln: 1.402 ± 0.05
1.212MetArg: 1.212 ± 0.047
1.531MetSer: 1.531 ± 0.049
1.431MetThr: 1.431 ± 0.05
1.499MetVal: 1.499 ± 0.045
0.228MetTrp: 0.228 ± 0.018
0.489MetTyr: 0.489 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
4.312AsnAla: 4.312 ± 0.136
0.423AsnCys: 0.423 ± 0.025
2.17AsnAsp: 2.17 ± 0.056
2.699AsnGlu: 2.699 ± 0.077
1.792AsnPhe: 1.792 ± 0.052
3.416AsnGly: 3.416 ± 0.102
0.977AsnHis: 0.977 ± 0.037
2.947AsnIle: 2.947 ± 0.072
2.507AsnLys: 2.507 ± 0.075
4.829AsnLeu: 4.829 ± 0.099
1.034AsnMet: 1.034 ± 0.04
2.167AsnAsn: 2.167 ± 0.085
2.444AsnPro: 2.444 ± 0.05
2.406AsnGln: 2.406 ± 0.073
2.222AsnArg: 2.222 ± 0.057
2.523AsnSer: 2.523 ± 0.087
2.176AsnThr: 2.176 ± 0.072
2.977AsnVal: 2.977 ± 0.083
0.668AsnTrp: 0.668 ± 0.036
1.565AsnTyr: 1.565 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
3.175ProAla: 3.175 ± 0.084
0.362ProCys: 0.362 ± 0.024
1.841ProAsp: 1.841 ± 0.058
2.901ProGlu: 2.901 ± 0.071
1.871ProPhe: 1.871 ± 0.059
1.749ProGly: 1.749 ± 0.056
0.959ProHis: 0.959 ± 0.037
2.591ProIle: 2.591 ± 0.075
2.021ProLys: 2.021 ± 0.063
4.354ProLeu: 4.354 ± 0.091
1.043ProMet: 1.043 ± 0.044
1.993ProAsn: 1.993 ± 0.057
1.227ProPro: 1.227 ± 0.049
2.412ProGln: 2.412 ± 0.063
1.491ProArg: 1.491 ± 0.06
2.129ProSer: 2.129 ± 0.059
2.251ProThr: 2.251 ± 0.065
2.563ProVal: 2.563 ± 0.075
0.446ProTrp: 0.446 ± 0.025
1.415ProTyr: 1.415 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
5.018GlnAla: 5.018 ± 0.13
0.399GlnCys: 0.399 ± 0.027
2.048GlnAsp: 2.048 ± 0.053
2.571GlnGlu: 2.571 ± 0.082
1.982GlnPhe: 1.982 ± 0.058
3.427GlnGly: 3.427 ± 0.08
1.17GlnHis: 1.17 ± 0.041
3.106GlnIle: 3.106 ± 0.083
2.67GlnLys: 2.67 ± 0.066
5.318GlnLeu: 5.318 ± 0.126
1.129GlnMet: 1.129 ± 0.046
2.194GlnAsn: 2.194 ± 0.061
1.929GlnPro: 1.929 ± 0.059
3.658GlnGln: 3.658 ± 0.108
2.54GlnArg: 2.54 ± 0.081
2.745GlnSer: 2.745 ± 0.065
2.534GlnThr: 2.534 ± 0.076
2.949GlnVal: 2.949 ± 0.063
0.7GlnTrp: 0.7 ± 0.033
1.562GlnTyr: 1.562 ± 0.055
0.0GlnXaa: 0.0 ± 0.0
Arg
3.664ArgAla: 3.664 ± 0.082
0.475ArgCys: 0.475 ± 0.032
2.379ArgAsp: 2.379 ± 0.06
3.118ArgGlu: 3.118 ± 0.078
2.316ArgPhe: 2.316 ± 0.061
2.795ArgGly: 2.795 ± 0.074
1.129ArgHis: 1.129 ± 0.044
3.17ArgIle: 3.17 ± 0.073
2.438ArgLys: 2.438 ± 0.062
5.535ArgLeu: 5.535 ± 0.107
1.011ArgMet: 1.011 ± 0.038
2.089ArgAsn: 2.089 ± 0.056
1.724ArgPro: 1.724 ± 0.056
2.612ArgGln: 2.612 ± 0.071
2.347ArgArg: 2.347 ± 0.072
2.301ArgSer: 2.301 ± 0.065
2.047ArgThr: 2.047 ± 0.064
2.977ArgVal: 2.977 ± 0.071
0.633ArgTrp: 0.633 ± 0.031
1.92ArgTyr: 1.92 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
5.855SerAla: 5.855 ± 0.48
0.524SerCys: 0.524 ± 0.031
2.824SerAsp: 2.824 ± 0.063
3.246SerGlu: 3.246 ± 0.074
2.485SerPhe: 2.485 ± 0.068
4.606SerGly: 4.606 ± 0.091
1.295SerHis: 1.295 ± 0.053
3.417SerIle: 3.417 ± 0.073
2.81SerLys: 2.81 ± 0.073
6.323SerLeu: 6.323 ± 0.104
1.344SerMet: 1.344 ± 0.045
2.308SerAsn: 2.308 ± 0.063
2.241SerPro: 2.241 ± 0.069
2.765SerGln: 2.765 ± 0.066
2.827SerArg: 2.827 ± 0.063
3.669SerSer: 3.669 ± 0.417
2.854SerThr: 2.854 ± 0.098
3.851SerVal: 3.851 ± 0.084
0.704SerTrp: 0.704 ± 0.037
1.836SerTyr: 1.836 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
5.277ThrAla: 5.277 ± 0.215
0.416ThrCys: 0.416 ± 0.027
2.596ThrAsp: 2.596 ± 0.069
3.093ThrGlu: 3.093 ± 0.073
2.066ThrPhe: 2.066 ± 0.062
3.671ThrGly: 3.671 ± 0.099
1.054ThrHis: 1.054 ± 0.042
3.174ThrIle: 3.174 ± 0.073
2.222ThrLys: 2.222 ± 0.068
5.728ThrLeu: 5.728 ± 0.111
0.983ThrMet: 0.983 ± 0.042
1.945ThrAsn: 1.945 ± 0.061
2.482ThrPro: 2.482 ± 0.073
2.295ThrGln: 2.295 ± 0.064
2.042ThrArg: 2.042 ± 0.06
2.493ThrSer: 2.493 ± 0.076
2.65ThrThr: 2.65 ± 0.075
3.519ThrVal: 3.519 ± 0.081
0.505ThrTrp: 0.505 ± 0.03
1.504ThrTyr: 1.504 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
6.21ValAla: 6.21 ± 0.117
0.715ValCys: 0.715 ± 0.033
3.587ValAsp: 3.587 ± 0.077
4.176ValGlu: 4.176 ± 0.095
2.575ValPhe: 2.575 ± 0.081
4.799ValGly: 4.799 ± 0.094
1.129ValHis: 1.129 ± 0.044
4.459ValIle: 4.459 ± 0.092
3.94ValLys: 3.94 ± 0.083
7.003ValLeu: 7.003 ± 0.101
1.733ValMet: 1.733 ± 0.058
2.96ValAsn: 2.96 ± 0.083
2.452ValPro: 2.452 ± 0.064
2.577ValGln: 2.577 ± 0.069
3.05ValArg: 3.05 ± 0.071
3.982ValSer: 3.982 ± 0.083
3.373ValThr: 3.373 ± 0.077
4.807ValVal: 4.807 ± 0.099
0.696ValTrp: 0.696 ± 0.033
1.79ValTyr: 1.79 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.964TrpAla: 0.964 ± 0.042
0.147TrpCys: 0.147 ± 0.014
0.584TrpAsp: 0.584 ± 0.03
0.533TrpGlu: 0.533 ± 0.026
0.619TrpPhe: 0.619 ± 0.035
0.757TrpGly: 0.757 ± 0.037
0.334TrpHis: 0.334 ± 0.022
0.7TrpIle: 0.7 ± 0.032
0.571TrpLys: 0.571 ± 0.032
1.809TrpLeu: 1.809 ± 0.059
0.313TrpMet: 0.313 ± 0.023
0.473TrpAsn: 0.473 ± 0.027
0.299TrpPro: 0.299 ± 0.019
1.092TrpGln: 1.092 ± 0.042
0.693TrpArg: 0.693 ± 0.035
0.624TrpSer: 0.624 ± 0.031
0.465TrpThr: 0.465 ± 0.03
0.739TrpVal: 0.739 ± 0.034
0.204TrpTrp: 0.204 ± 0.022
0.331TrpTyr: 0.331 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.711TyrAla: 2.711 ± 0.062
0.426TyrCys: 0.426 ± 0.028
1.603TyrAsp: 1.603 ± 0.052
1.507TyrGlu: 1.507 ± 0.05
1.621TyrPhe: 1.621 ± 0.052
2.194TyrGly: 2.194 ± 0.068
0.791TyrHis: 0.791 ± 0.042
1.659TyrIle: 1.659 ± 0.061
1.371TyrLys: 1.371 ± 0.047
3.664TyrLeu: 3.664 ± 0.083
0.589TyrMet: 0.589 ± 0.032
1.255TyrAsn: 1.255 ± 0.044
1.455TyrPro: 1.455 ± 0.046
2.042TyrGln: 2.042 ± 0.067
1.898TyrArg: 1.898 ± 0.058
1.82TyrSer: 1.82 ± 0.055
1.323TyrThr: 1.323 ± 0.045
1.909TyrVal: 1.909 ± 0.058
0.557TyrTrp: 0.557 ± 0.029
1.086TyrTyr: 1.086 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2390 proteins (631774 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski