Labrenzia alexandrii
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4993 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M7AGQ4|A0A0M7AGQ4_9RHOB Uncharacterized protein OS=Labrenzia alexandrii OX=388408 GN=LAX5112_03825 PE=4 SV=1
MM1 pKa = 7.89 ADD3 pKa = 3.53 NSDD6 pKa = 3.69 FHH8 pKa = 5.82 QTGDD12 pKa = 4.45 EE13 pKa = 4.93 DD14 pKa = 6.41 DD15 pKa = 3.85 PTQNGNGEE23 pKa = 4.17 AEE25 pKa = 4.03 EE26 pKa = 5.42 AIAQLFDD33 pKa = 4.93 DD34 pKa = 4.46 YY35 pKa = 11.8 QSGFNDD41 pKa = 2.99 YY42 pKa = 11.51 DD43 pKa = 3.32 MEE45 pKa = 5.04 RR46 pKa = 11.84 ICDD49 pKa = 3.94 CFALPTIIWQHH60 pKa = 4.99 EE61 pKa = 4.24 KK62 pKa = 10.88 GHH64 pKa = 5.72 VFNDD68 pKa = 3.56 DD69 pKa = 3.36 EE70 pKa = 4.25 EE71 pKa = 6.21 LIEE74 pKa = 4.69 NIEE77 pKa = 4.24 VLLKK81 pKa = 10.68 ALEE84 pKa = 4.29 KK85 pKa = 10.77 EE86 pKa = 4.8 GVSHH90 pKa = 7.61 SDD92 pKa = 3.42 FQVVSSHH99 pKa = 5.33 VSGPTALVTLDD110 pKa = 3.75 WSQEE114 pKa = 4.24 SADD117 pKa = 3.74 GEE119 pKa = 4.51 AVFEE123 pKa = 4.4 FTCHH127 pKa = 5.13 YY128 pKa = 10.88 QLIQDD133 pKa = 4.04 GQDD136 pKa = 2.53 WMIAGIVNEE145 pKa = 4.14
Molecular weight: 16.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.541
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.859
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A0M7APZ5|A0A0M7APZ5_9RHOB (S)-sulfolactate dehydrogenase OS=Labrenzia alexandrii OX=388408 GN=slcC PE=3 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 FAFIAEE8 pKa = 4.23 NQGVLPASRR17 pKa = 11.84 LCQIMNVSPRR27 pKa = 11.84 GYY29 pKa = 9.65 RR30 pKa = 11.84 SWRR33 pKa = 11.84 DD34 pKa = 3.07 RR35 pKa = 11.84 PVSDD39 pKa = 3.91 RR40 pKa = 11.84 QRR42 pKa = 11.84 KK43 pKa = 8.95 DD44 pKa = 3.45 LVVLAHH50 pKa = 6.47 IRR52 pKa = 11.84 EE53 pKa = 4.12 QFRR56 pKa = 11.84 LSLGSYY62 pKa = 8.66 GRR64 pKa = 11.84 PRR66 pKa = 11.84 MTEE69 pKa = 3.72 EE70 pKa = 4.02 LKK72 pKa = 11.09 EE73 pKa = 4.02 LGLDD77 pKa = 2.92 IGHH80 pKa = 6.48 RR81 pKa = 11.84 RR82 pKa = 11.84 VGRR85 pKa = 11.84 LMRR88 pKa = 11.84 EE89 pKa = 3.46 NAITVKK95 pKa = 10.59 GNKK98 pKa = 8.97 KK99 pKa = 10.19 FKK101 pKa = 10.19 ATTDD105 pKa = 3.19 SNHH108 pKa = 6.68 RR109 pKa = 11.84 FNIAPNLLKK118 pKa = 10.24 RR119 pKa = 11.84 DD120 pKa = 4.65 FSADD124 pKa = 3.25 RR125 pKa = 11.84 PNRR128 pKa = 11.84 KK129 pKa = 7.97 WAGDD133 pKa = 3.22 ISYY136 pKa = 10.33 VWTRR140 pKa = 11.84 EE141 pKa = 3.36 GWLYY145 pKa = 10.97 LAVILDD151 pKa = 3.56 LHH153 pKa = 5.84 SRR155 pKa = 11.84 RR156 pKa = 11.84 VIGWAVSNRR165 pKa = 11.84 LKK167 pKa = 10.6 RR168 pKa = 11.84 DD169 pKa = 3.26 LAIRR173 pKa = 11.84 ALNMAIALRR182 pKa = 11.84 RR183 pKa = 11.84 PPKK186 pKa = 10.5 GCIHH190 pKa = 5.84 HH191 pKa = 6.77 TDD193 pKa = 3.63 RR194 pKa = 11.84 GSQYY198 pKa = 10.54 CSHH201 pKa = 8.02 DD202 pKa = 3.63 YY203 pKa = 10.89 QKK205 pKa = 11.49 LLRR208 pKa = 11.84 QHH210 pKa = 6.85 GFQISMSGTGNCFDD224 pKa = 3.78 NAAAEE229 pKa = 4.54 TFFKK233 pKa = 10.31 TIKK236 pKa = 10.85 AEE238 pKa = 4.19 LLWQKK243 pKa = 10.29 SWACRR248 pKa = 11.84 RR249 pKa = 11.84 EE250 pKa = 3.72 AEE252 pKa = 4.19 MAIFEE257 pKa = 4.85 YY258 pKa = 10.61 INGFYY263 pKa = 10.56 NPRR266 pKa = 11.84 RR267 pKa = 11.84 RR268 pKa = 11.84 HH269 pKa = 4.97 SALAA273 pKa = 3.76
Molecular weight: 31.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.677
IPC_protein 10.555
Toseland 10.701
ProMoST 10.438
Dawson 10.789
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 10.965
Grimsley 10.847
Solomon 10.921
Lehninger 10.891
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.111
Sillero 10.73
Patrickios 10.657
IPC_peptide 10.935
IPC2_peptide 9.633
IPC2.peptide.svr19 8.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4993
0
4993
1582945
29
3368
317.0
34.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.171 ± 0.043
0.867 ± 0.011
6.068 ± 0.033
6.11 ± 0.037
4.03 ± 0.022
8.272 ± 0.03
1.979 ± 0.018
5.517 ± 0.028
3.947 ± 0.028
10.013 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.549 ± 0.019
3.028 ± 0.023
4.824 ± 0.028
3.358 ± 0.021
5.911 ± 0.035
5.863 ± 0.023
5.62 ± 0.027
7.26 ± 0.027
1.27 ± 0.012
2.341 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here