Tessaracoccus flavescens
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3222 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Q2CV15|A0A1Q2CV15_9ACTN 30S ribosomal protein S13 OS=Tessaracoccus flavescens OX=399497 GN=rpsM PE=3 SV=1
MM1 pKa = 7.35 RR2 pKa = 11.84 TLLIATASLATLIAAGACTADD23 pKa = 3.69 QPAPGPQPASTSAAAVDD40 pKa = 3.67 VDD42 pKa = 5.6 DD43 pKa = 6.68 FLAQHH48 pKa = 6.81 DD49 pKa = 3.95 LAGRR53 pKa = 11.84 SVTEE57 pKa = 3.67 IVEE60 pKa = 4.32 ALDD63 pKa = 3.49 GTNEE67 pKa = 3.79 DD68 pKa = 4.73 RR69 pKa = 11.84 EE70 pKa = 4.2 NGPYY74 pKa = 10.68 GSVLPGEE81 pKa = 5.41 LILADD86 pKa = 3.94 EE87 pKa = 4.77 KK88 pKa = 11.18 QQVSLPIEE96 pKa = 4.03 DD97 pKa = 4.3 AFYY100 pKa = 11.17 LSVAPYY106 pKa = 8.7 VTGTHH111 pKa = 5.42 EE112 pKa = 4.6 CYY114 pKa = 10.49 NHH116 pKa = 6.44 NLASCQGEE124 pKa = 4.11 LAQQEE129 pKa = 4.2 FDD131 pKa = 3.57 VTIVGEE137 pKa = 4.26 DD138 pKa = 3.38 GQEE141 pKa = 3.86 IFDD144 pKa = 4.26 GQVTTAPNGFAGFWLPKK161 pKa = 9.92 EE162 pKa = 3.78 ITATVTVEE170 pKa = 3.65 HH171 pKa = 7.49 DD172 pKa = 3.11 GRR174 pKa = 11.84 TATTEE179 pKa = 3.94 VSTGAEE185 pKa = 4.41 DD186 pKa = 3.82 PTCITTMQLAA196 pKa = 3.65
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.643
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.554
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.681
EMBOSS 3.732
Sillero 3.948
Patrickios 1.036
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A1Q2CW34|A0A1Q2CW34_9ACTN Uncharacterized protein OS=Tessaracoccus flavescens OX=399497 GN=BW733_05200 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.72 HH17 pKa = 5.74 RR18 pKa = 11.84 KK19 pKa = 7.87 LLKK22 pKa = 8.37 RR23 pKa = 11.84 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3222
0
3222
1009990
33
2120
313.5
33.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.299 ± 0.052
0.706 ± 0.012
6.284 ± 0.034
6.102 ± 0.045
3.011 ± 0.024
8.734 ± 0.04
2.026 ± 0.023
4.339 ± 0.033
2.483 ± 0.032
10.151 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.018 ± 0.018
2.171 ± 0.025
5.387 ± 0.034
3.091 ± 0.023
7.306 ± 0.055
5.676 ± 0.03
6.039 ± 0.05
8.576 ± 0.037
1.588 ± 0.019
2.013 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here