Dorcoceras hygrometricum
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45898 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z7D8A3|A0A2Z7D8A3_9LAMI 3-hydroxy-3-methylglutaryl coenzyme A synthase OS=Dorcoceras hygrometricum OX=472368 GN=F511_26294 PE=3 SV=1
MM1 pKa = 7.47 VDD3 pKa = 3.11 SSTIAATGFFDD14 pKa = 5.83 DD15 pKa = 5.53 WILCAWLQLFDD26 pKa = 3.75 TSAFLASGFPATGYY40 pKa = 10.77 AKK42 pKa = 10.08 HH43 pKa = 6.28 CRR45 pKa = 11.84 SLLIVDD51 pKa = 3.83 VTADD55 pKa = 4.79 FIITDD60 pKa = 3.87 PALALLFTTADD71 pKa = 4.15 CDD73 pKa = 5.66 DD74 pKa = 3.43 ITADD78 pKa = 3.8 VNIADD83 pKa = 3.47 FRR85 pKa = 11.84 YY86 pKa = 8.35 RR87 pKa = 11.84 TSCC90 pKa = 3.64
Molecular weight: 9.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.503
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.808
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.266
Thurlkill 3.605
EMBOSS 3.808
Sillero 3.884
Patrickios 0.782
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A2Z7CHT6|A0A2Z7CHT6_9LAMI Uncharacterized protein OS=Dorcoceras hygrometricum OX=472368 GN=F511_18876 PE=4 SV=1
MM1 pKa = 7.28 IVAFFARR8 pKa = 11.84 NQQVPFFARR17 pKa = 11.84 NQQVPFFARR26 pKa = 11.84 NQQVPFFARR35 pKa = 11.84 NQQVPFFARR44 pKa = 11.84 NQQVPFFARR53 pKa = 11.84 NQQVPFFARR62 pKa = 11.84 NQQVPFFARR71 pKa = 11.84 NQQVPFFARR80 pKa = 11.84 NQQVPFFARR89 pKa = 11.84 NQQVPFFARR98 pKa = 11.84 NQQVPFFARR107 pKa = 11.84 NQQVPFFARR116 pKa = 11.84 NQQVPFFARR125 pKa = 11.84 NQQVPFFARR134 pKa = 11.84 NQQVPFFARR143 pKa = 11.84 NQQVPFFARR152 pKa = 11.84 NQQVPFFARR161 pKa = 11.84 NQQVPFFARR170 pKa = 11.84 NQQVPFFARR179 pKa = 11.84 NQQVPFFARR188 pKa = 11.84 NQQVPFFARR197 pKa = 11.84 NQQVPFFARR206 pKa = 11.84 NQQVLLSEE214 pKa = 4.57 KK215 pKa = 10.17 PAGASS220 pKa = 3.18
Molecular weight: 26.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.242
IPC_protein 12.866
Toseland 13.027
ProMoST 13.525
Dawson 13.027
Bjellqvist 13.027
Wikipedia 13.495
Rodwell 12.544
Grimsley 13.056
Solomon 13.525
Lehninger 13.422
Nozaki 13.027
DTASelect 13.027
Thurlkill 13.027
EMBOSS 13.525
Sillero 13.027
Patrickios 12.266
IPC_peptide 13.525
IPC2_peptide 12.515
IPC2.peptide.svr19 9.222
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
45898
0
45898
14407823
50
4973
313.9
35.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.416 ± 0.016
1.793 ± 0.006
5.452 ± 0.009
6.422 ± 0.014
3.962 ± 0.008
6.077 ± 0.012
2.359 ± 0.005
5.295 ± 0.009
5.693 ± 0.011
9.152 ± 0.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.004
3.957 ± 0.008
4.914 ± 0.01
4.065 ± 0.009
6.243 ± 0.012
9.216 ± 0.016
5.248 ± 0.01
6.686 ± 0.011
1.236 ± 0.004
2.416 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here