Litoricola lipolytica
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2331 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2Q8T5|A0A5Q2Q8T5_9GAMM Saccharopine dehydrogenase family protein OS=Litoricola lipolytica OX=418701 GN=GH975_11525 PE=4 SV=1
MM1 pKa = 7.15 PTVKK5 pKa = 10.2 FEE7 pKa = 4.39 YY8 pKa = 10.26 GDD10 pKa = 3.35 EE11 pKa = 4.32 TFEE14 pKa = 4.02 VTAEE18 pKa = 3.85 IGEE21 pKa = 4.53 SVMMAAARR29 pKa = 11.84 NRR31 pKa = 11.84 VPGIDD36 pKa = 3.63 ADD38 pKa = 4.08 CGGGCSCATCHH49 pKa = 6.58 VYY51 pKa = 10.73 APDD54 pKa = 3.94 GAALPAPSDD63 pKa = 3.71 MEE65 pKa = 5.53 KK66 pKa = 11.3 DD67 pKa = 3.34 MLDD70 pKa = 3.49 FADD73 pKa = 4.51 ALTDD77 pKa = 3.94 ASRR80 pKa = 11.84 LSCQIVVTPSMDD92 pKa = 2.64 GVTYY96 pKa = 9.05 VIPKK100 pKa = 9.82 LL101 pKa = 3.64
Molecular weight: 10.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 4.024
IPC_protein 3.961
Toseland 3.757
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.783
Grimsley 3.668
Solomon 3.935
Lehninger 3.897
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.897
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.98
Protein with the highest isoelectric point:
>tr|A0A5Q2QAU6|A0A5Q2QAU6_9GAMM Uncharacterized protein OS=Litoricola lipolytica OX=418701 GN=GH975_02045 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 8.26 RR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.39 NGRR28 pKa = 11.84 LVLKK32 pKa = 10.32 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.89 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2331
0
2331
732204
31
4212
314.1
34.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.819 ± 0.058
0.986 ± 0.018
6.188 ± 0.045
5.133 ± 0.051
3.487 ± 0.035
8.288 ± 0.047
2.164 ± 0.024
4.991 ± 0.033
3.024 ± 0.035
10.754 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.023
2.957 ± 0.032
4.655 ± 0.034
4.212 ± 0.033
6.346 ± 0.052
5.794 ± 0.037
5.223 ± 0.045
7.657 ± 0.045
1.496 ± 0.022
2.252 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here