Agrilactobacillus composti DSM 18527 = JCM 14202
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3260 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1Y2M9|A0A0R1Y2M9_9LACO Glycosyl transferase 2 family protein OS=Agrilactobacillus composti DSM 18527 = JCM 14202 OX=1423734 GN=FC83_GL003004 PE=4 SV=1
MM1 pKa = 7.6 ASVKK5 pKa = 10.23 IIEE8 pKa = 4.35 TSVASGNTGGDD19 pKa = 3.38 MYY21 pKa = 11.1 DD22 pKa = 4.02 SPNGTYY28 pKa = 10.06 IKK30 pKa = 10.73 ALALNTDD37 pKa = 2.96 WEE39 pKa = 4.54 YY40 pKa = 11.73 FDD42 pKa = 3.89 TQTVDD47 pKa = 3.12 GQIWYY52 pKa = 9.58 DD53 pKa = 3.49 LGGDD57 pKa = 3.38 QWISEE62 pKa = 4.37 IYY64 pKa = 10.0 AAPNINDD71 pKa = 3.69 LEE73 pKa = 4.45 VRR75 pKa = 11.84 SGTAKK80 pKa = 10.59 VADD83 pKa = 3.98 FTTATVYY90 pKa = 10.19 YY91 pKa = 9.85 SPNGSEE97 pKa = 4.0 NTQDD101 pKa = 3.67 NPTPLPEE108 pKa = 4.22 NSEE111 pKa = 3.8 WAYY114 pKa = 11.48 NKK116 pKa = 10.4 AATDD120 pKa = 3.67 SEE122 pKa = 4.39 GTIWFDD128 pKa = 3.06 VGRR131 pKa = 11.84 SQWVTTQEE139 pKa = 4.44 VVDD142 pKa = 4.36 EE143 pKa = 4.57 DD144 pKa = 3.83 AGGDD148 pKa = 3.91 IEE150 pKa = 4.48 WSGSGIFQYY159 pKa = 8.49 PTYY162 pKa = 10.89 SDD164 pKa = 3.42 GGINADD170 pKa = 4.68 MIRR173 pKa = 11.84 QAASDD178 pKa = 4.06 MYY180 pKa = 10.84 TSVSDD185 pKa = 3.91 DD186 pKa = 3.67 QINTIMAVAEE196 pKa = 4.3 HH197 pKa = 6.69 EE198 pKa = 4.57 SGNNSNKK205 pKa = 9.26 INNWDD210 pKa = 3.41 KK211 pKa = 10.94 NAQEE215 pKa = 4.12 GHH217 pKa = 6.38 PSKK220 pKa = 11.03 GVLQFVGYY228 pKa = 8.27 TFDD231 pKa = 3.86 HH232 pKa = 5.7 YY233 pKa = 11.17 HH234 pKa = 5.87 VANHH238 pKa = 6.33 DD239 pKa = 4.62 NIWSSMDD246 pKa = 3.15 QLYY249 pKa = 11.26 ALFNDD254 pKa = 4.11 ATWASDD260 pKa = 2.83 ISLGGWAPNGPVRR273 pKa = 11.84 FPAA276 pKa = 4.36
Molecular weight: 30.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.846
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 0.922
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|X0QHW8|X0QHW8_9LACO Uncharacterized protein OS=Agrilactobacillus composti DSM 18527 = JCM 14202 OX=1423734 GN=FC83_GL001830 PE=4 SV=1
MM1 pKa = 7.2 FHH3 pKa = 7.3 LIWVLIIGALIGVIAGAITSRR24 pKa = 11.84 GMPFGWIGNILAGLIGAWLGQRR46 pKa = 11.84 LLGTWGPSLAGMALIPAIIGAIILVLVVSLILGATTKK83 pKa = 10.41 RR84 pKa = 11.84 RR85 pKa = 11.84 AA86 pKa = 3.17
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 10.716
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.954
Rodwell 12.106
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.901
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3260
0
3260
1004349
49
1815
308.1
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.184 ± 0.047
0.534 ± 0.012
5.667 ± 0.043
4.379 ± 0.036
4.356 ± 0.038
6.881 ± 0.035
2.124 ± 0.02
7.004 ± 0.041
5.849 ± 0.037
10.307 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.505 ± 0.021
4.591 ± 0.033
4.046 ± 0.029
5.268 ± 0.046
3.754 ± 0.031
5.379 ± 0.037
6.59 ± 0.048
6.969 ± 0.032
1.062 ± 0.017
3.539 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here