Cohaesibacter marisflavi
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4794 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5CN02|A0A1I5CN02_9RHIZ 2-dehydro-3-deoxygluconokinase OS=Cohaesibacter marisflavi OX=655353 GN=SAMN04488056_102284 PE=4 SV=1
MM1 pKa = 8.03 SLMGATNAAITGLAAQSQALSNVSNNLANSDD32 pKa = 3.42 TTAYY36 pKa = 9.47 KK37 pKa = 10.47 ASKK40 pKa = 9.36 TSFSSLVAGSGNNKK54 pKa = 9.96 SGGVHH59 pKa = 7.49 ASNTTNNTAQGLVTDD74 pKa = 4.67 SAISTHH80 pKa = 6.83 LAIQGNGYY88 pKa = 9.63 FVVSEE93 pKa = 4.33 EE94 pKa = 4.58 GDD96 pKa = 3.5 TSSRR100 pKa = 11.84 YY101 pKa = 5.33 YY102 pKa = 9.63 TRR104 pKa = 11.84 NGEE107 pKa = 4.07 FAVDD111 pKa = 3.22 NDD113 pKa = 4.33 GYY115 pKa = 11.53 LMNGDD120 pKa = 4.15 YY121 pKa = 11.13 YY122 pKa = 11.54 LLGWATDD129 pKa = 3.25 ADD131 pKa = 4.31 GNVAGGASSVNLDD144 pKa = 4.27 KK145 pKa = 10.82 IDD147 pKa = 4.05 LSSIQSSAQATSEE160 pKa = 3.95 ASIQATLPGDD170 pKa = 3.42 AVLNSTFEE178 pKa = 4.31 SSFEE182 pKa = 4.34 VYY184 pKa = 10.48 DD185 pKa = 3.88 SLGTASTITATYY197 pKa = 9.7 EE198 pKa = 3.65 KK199 pKa = 9.43 TAQNTWTITYY209 pKa = 9.32 SDD211 pKa = 4.5 PVSADD216 pKa = 2.95 TSTTTGSVTSSPITVQFNNDD236 pKa = 2.45 GSLASVSGTDD246 pKa = 6.22 LDD248 pKa = 4.1 ITWNTGAAASSIKK261 pKa = 10.45 LDD263 pKa = 3.63 LGTVGGSDD271 pKa = 3.87 GLTQYY276 pKa = 11.18 SSNDD280 pKa = 3.3 VEE282 pKa = 4.58 NADD285 pKa = 3.95 IDD287 pKa = 4.17 VKK289 pKa = 11.16 SIIVDD294 pKa = 3.35 GRR296 pKa = 11.84 AYY298 pKa = 9.87 GTVDD302 pKa = 4.43 DD303 pKa = 5.79 IEE305 pKa = 5.79 IEE307 pKa = 4.14 TDD309 pKa = 3.33 GSVVATFSNGEE320 pKa = 3.66 TRR322 pKa = 11.84 NIYY325 pKa = 9.7 KK326 pKa = 10.2 IPVATFINSDD336 pKa = 3.55 GLTEE340 pKa = 4.78 DD341 pKa = 3.34 SDD343 pKa = 4.46 GIYY346 pKa = 10.53 AISTYY351 pKa = 10.76 SGNATLHH358 pKa = 5.4 TAGTGSAGSLKK369 pKa = 10.58 SSSLEE374 pKa = 3.84 SSTVDD379 pKa = 2.9 TSTEE383 pKa = 3.95 FASMLSAQQAYY394 pKa = 10.4 SSASQIISTAGDD406 pKa = 3.45 MFDD409 pKa = 5.09 SLLQAVRR416 pKa = 3.68
Molecular weight: 42.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.91
Patrickios 1.227
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A1I5F2R4|A0A1I5F2R4_9RHIZ Transcriptional regulator AraC family OS=Cohaesibacter marisflavi OX=655353 GN=SAMN04488056_103462 PE=4 SV=1
MM1 pKa = 8.21 DD2 pKa = 4.9 MFPEE6 pKa = 3.92 KK7 pKa = 10.38 AARR10 pKa = 11.84 FKK12 pKa = 10.99 GIRR15 pKa = 11.84 AGASTSFFTAALGSIVSRR33 pKa = 11.84 TSARR37 pKa = 11.84 GAVHH41 pKa = 6.52 PRR43 pKa = 11.84 LSGHH47 pKa = 5.7 SLYY50 pKa = 10.57 LRR52 pKa = 11.84 HH53 pKa = 6.16 PVKK56 pKa = 10.77 GDD58 pKa = 3.44 FAQWVRR64 pKa = 11.84 LRR66 pKa = 11.84 TASAGFLKK74 pKa = 10.08 PWEE77 pKa = 4.3 PLWPRR82 pKa = 11.84 DD83 pKa = 3.68 DD84 pKa = 3.5 LTMRR88 pKa = 11.84 GYY90 pKa = 10.65 RR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 LDD95 pKa = 3.05 QYY97 pKa = 11.09 RR98 pKa = 11.84 RR99 pKa = 11.84 DD100 pKa = 3.84 RR101 pKa = 11.84 KK102 pKa = 8.44 TGRR105 pKa = 11.84 ALPYY109 pKa = 10.27 LIFDD113 pKa = 3.84 ARR115 pKa = 11.84 DD116 pKa = 3.12 NRR118 pKa = 11.84 LLGGVNVSNIRR129 pKa = 11.84 RR130 pKa = 11.84 GICQTATIGYY140 pKa = 8.96 WMGEE144 pKa = 3.78 NHH146 pKa = 7.23 AGQGNMSRR154 pKa = 11.84 ALALLLPYY162 pKa = 10.85 LFDD165 pKa = 3.85 CQGLHH170 pKa = 6.98 RR171 pKa = 11.84 IEE173 pKa = 4.6 AACLPTNSASISVLKK188 pKa = 10.8 KK189 pKa = 10.66 SGFQLEE195 pKa = 4.54 GKK197 pKa = 9.61 ARR199 pKa = 11.84 SYY201 pKa = 11.56 LCINGQWEE209 pKa = 4.1 DD210 pKa = 3.6 HH211 pKa = 6.55 LLFSALRR218 pKa = 11.84 SDD220 pKa = 4.37 VIMSVGFF227 pKa = 3.59
Molecular weight: 25.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.648
IPC_protein 10.511
Toseland 10.482
ProMoST 10.248
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.774
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.438
IPC_peptide 10.73
IPC2_peptide 9.531
IPC2.peptide.svr19 8.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4794
0
4794
1536085
10
2835
320.4
35.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.547 ± 0.038
0.942 ± 0.012
5.92 ± 0.033
6.174 ± 0.038
3.999 ± 0.022
7.912 ± 0.039
2.053 ± 0.016
6.024 ± 0.027
4.529 ± 0.027
10.1 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.86 ± 0.017
3.198 ± 0.02
4.556 ± 0.028
3.476 ± 0.02
5.561 ± 0.03
6.346 ± 0.031
5.215 ± 0.021
6.906 ± 0.027
1.218 ± 0.016
2.465 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here