Apiotrichum porosum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A427Y9P2|A0A427Y9P2_9TREE TAFII55_N domain-containing protein OS=Apiotrichum porosum OX=105984 GN=EHS24_000251 PE=3 SV=1
MM1 pKa = 7.46AMCGAGLDD9 pKa = 3.88GVVCMFAEE17 pKa = 4.08LHH19 pKa = 6.99DD20 pKa = 5.11PDD22 pKa = 4.9EE23 pKa = 4.84STGSATASADD33 pKa = 3.62DD34 pKa = 3.95GDD36 pKa = 4.26EE37 pKa = 4.38EE38 pKa = 4.54EE39 pKa = 4.8EE40 pKa = 3.92QDD42 pKa = 4.74LVYY45 pKa = 10.98SPSDD49 pKa = 3.51PSMVAADD56 pKa = 3.77QFPATAHH63 pKa = 6.17ASAFITPEE71 pKa = 3.89RR72 pKa = 11.84PASRR76 pKa = 11.84HH77 pKa = 5.8HH78 pKa = 6.64DD79 pKa = 3.56TPATAEE85 pKa = 4.03TDD87 pKa = 3.71STGSADD93 pKa = 3.52TTFFALLDD101 pKa = 3.75WAMGAASAGLDD112 pKa = 3.18GLVYY116 pKa = 10.43RR117 pKa = 11.84FAPLDD122 pKa = 4.13DD123 pKa = 5.45LDD125 pKa = 4.77APSPSAYY132 pKa = 9.98EE133 pKa = 3.61YY134 pKa = 11.08DD135 pKa = 3.98EE136 pKa = 4.44YY137 pKa = 11.62DD138 pKa = 4.94EE139 pKa = 5.87YY140 pKa = 11.58DD141 pKa = 3.05QCDD144 pKa = 4.9DD145 pKa = 4.25YY146 pKa = 11.93DD147 pKa = 3.58QCDD150 pKa = 4.49DD151 pKa = 3.72YY152 pKa = 12.05AQADD156 pKa = 3.42QHH158 pKa = 6.41DD159 pKa = 4.27QNDD162 pKa = 3.5QNDD165 pKa = 3.31QCGFEE170 pKa = 4.22RR171 pKa = 11.84AYY173 pKa = 9.77TDD175 pKa = 4.27CDD177 pKa = 3.96DD178 pKa = 3.92EE179 pKa = 4.5VQEE182 pKa = 4.13EE183 pKa = 4.49EE184 pKa = 4.26EE185 pKa = 4.49RR186 pKa = 11.84DD187 pKa = 3.63LEE189 pKa = 4.78FISPPRR195 pKa = 11.84TPGHH199 pKa = 6.35TIVIDD204 pKa = 3.82WSCLRR209 pKa = 11.84DD210 pKa = 3.56WVARR214 pKa = 11.84TIQNGGEE221 pKa = 4.03PLYY224 pKa = 9.53PLQDD228 pKa = 2.91IAIRR232 pKa = 11.84RR233 pKa = 11.84TRR235 pKa = 11.84VDD237 pKa = 2.81PAAAVRR243 pKa = 11.84SDD245 pKa = 3.5VPEE248 pKa = 4.32EE249 pKa = 4.22ADD251 pKa = 4.2SPSQPSPTDD260 pKa = 2.96AGAEE264 pKa = 3.88IGSAFTTPARR274 pKa = 11.84LGEE277 pKa = 4.39GDD279 pKa = 3.46QGTPAMTNTASSSSSASDD297 pKa = 3.19AFF299 pKa = 3.84

Molecular weight: 32.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A427Y925|A0A427Y925_9TREE 60S ribosomal protein L35 OS=Apiotrichum porosum OX=105984 GN=RPL35 PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84QRR4 pKa = 11.84KK5 pKa = 8.6LNKK8 pKa = 8.91RR9 pKa = 11.84AARR12 pKa = 11.84HH13 pKa = 5.5GGQPGVGGAGMAAGGMHH30 pKa = 5.23QHH32 pKa = 6.14HH33 pKa = 7.03MGGGGGVGGQQHH45 pKa = 5.55MAGGGLAGQGGQHH58 pKa = 5.97MAGQGMGMQGGVQPGMGQPGMGQPGMGGQPGMGGQPGMGGQPGMGHH104 pKa = 5.17QQGGMGGPGMGRR116 pKa = 3.16

Molecular weight: 10.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9145

0

9145

4526824

50

4980

495.0

53.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.623 ± 0.03

1.05 ± 0.009

5.838 ± 0.019

5.659 ± 0.022

3.304 ± 0.016

7.286 ± 0.028

2.457 ± 0.014

4.073 ± 0.016

4.096 ± 0.021

8.665 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.01

3.09 ± 0.012

7.109 ± 0.035

3.554 ± 0.024

6.212 ± 0.025

7.984 ± 0.034

6.234 ± 0.02

6.736 ± 0.018

1.429 ± 0.01

2.45 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski