Silvanigrellales bacterium RF1110005

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Silvanigrellales; Silvanigrellaceae; Fluviispira

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P2VN09|A0A4P2VN09_9PROT Uncharacterized protein OS=Silvanigrellales bacterium RF1110005 OX=2493639 GN=JCM31447_28660 PE=4 SV=1
MM1 pKa = 7.62IDD3 pKa = 4.42DD4 pKa = 4.0YY5 pKa = 11.78FMNYY9 pKa = 10.17SILDD13 pKa = 4.01DD14 pKa = 4.31EE15 pKa = 5.92LEE17 pKa = 4.4SDD19 pKa = 5.46DD20 pKa = 5.5DD21 pKa = 4.33NEE23 pKa = 4.56SLGVRR28 pKa = 11.84TEE30 pKa = 3.74KK31 pKa = 10.74LIRR34 pKa = 11.84DD35 pKa = 3.49IDD37 pKa = 3.97SDD39 pKa = 4.36IEE41 pKa = 4.08NLTEE45 pKa = 3.89NEE47 pKa = 3.73FMDD50 pKa = 3.67IYY52 pKa = 11.17GVSTEE57 pKa = 3.64IAKK60 pKa = 9.63YY61 pKa = 10.07QGMTKK66 pKa = 10.29EE67 pKa = 4.44EE68 pKa = 4.24YY69 pKa = 10.39EE70 pKa = 4.16EE71 pKa = 5.18LYY73 pKa = 11.08SS74 pKa = 4.3

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P2VJP6|A0A4P2VJP6_9PROT PNPLA domain-containing protein OS=Silvanigrellales bacterium RF1110005 OX=2493639 GN=JCM31447_12010 PE=4 SV=1
MM1 pKa = 5.7VTRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.97KK7 pKa = 10.75AGTKK11 pKa = 9.28TKK13 pKa = 9.63TAGKK17 pKa = 9.64KK18 pKa = 9.03RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 7.97TSSKK25 pKa = 9.17KK26 pKa = 6.49TTATKK31 pKa = 10.42RR32 pKa = 11.84KK33 pKa = 8.88RR34 pKa = 11.84KK35 pKa = 8.12KK36 pKa = 8.37TAGKK40 pKa = 8.44KK41 pKa = 5.29TTATKK46 pKa = 10.46RR47 pKa = 11.84KK48 pKa = 8.88RR49 pKa = 11.84KK50 pKa = 8.12KK51 pKa = 8.37TAGKK55 pKa = 8.44KK56 pKa = 5.29TTATKK61 pKa = 10.46RR62 pKa = 11.84KK63 pKa = 8.88RR64 pKa = 11.84KK65 pKa = 8.12KK66 pKa = 8.37TAGKK70 pKa = 8.44KK71 pKa = 5.29TTATKK76 pKa = 10.42RR77 pKa = 11.84KK78 pKa = 9.09RR79 pKa = 11.84KK80 pKa = 8.45KK81 pKa = 7.09TTATKK86 pKa = 10.07TGVKK90 pKa = 8.03KK91 pKa = 9.25TKK93 pKa = 10.22RR94 pKa = 11.84KK95 pKa = 9.83AKK97 pKa = 10.1RR98 pKa = 11.84KK99 pKa = 7.09TKK101 pKa = 10.48LSVVSKK107 pKa = 8.1TRR109 pKa = 11.84KK110 pKa = 9.37RR111 pKa = 11.84KK112 pKa = 7.41TKK114 pKa = 10.43RR115 pKa = 11.84KK116 pKa = 4.83TTARR120 pKa = 11.84KK121 pKa = 8.59VVARR125 pKa = 11.84KK126 pKa = 7.91TSDD129 pKa = 2.85KK130 pKa = 10.6HH131 pKa = 6.0LKK133 pKa = 9.45KK134 pKa = 10.74AGG136 pKa = 3.42

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

1068353

39

4399

337.3

38.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.356 ± 0.045

1.175 ± 0.015

4.73 ± 0.026

6.576 ± 0.046

5.403 ± 0.039

5.494 ± 0.042

1.886 ± 0.016

8.805 ± 0.048

8.254 ± 0.046

9.908 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.017

6.402 ± 0.055

3.472 ± 0.025

3.451 ± 0.025

3.668 ± 0.034

7.607 ± 0.042

4.771 ± 0.032

5.413 ± 0.034

0.971 ± 0.014

3.572 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski